ARASENS is an international phase III trial demonstrating the beneficial role of darolutamide,a novel antiandrogen,when added to docetaxel and androgen deprivation therapy(ADT),with improvement in overall survival in ...ARASENS is an international phase III trial demonstrating the beneficial role of darolutamide,a novel antiandrogen,when added to docetaxel and androgen deprivation therapy(ADT),with improvement in overall survival in men with metastatic castration-sensitive or hormone-sensitive prostate cancer.Darolutamide is a nonsteroidal androgen receptor antagonist that has been previously shown to improve metastasis-free survival in nonmetastatic castration-resistant prostate cancer.ARASENS showed that the risk of death was 32.5%lower in those who received additional darolutamide compared to ADT and docetaxel alone.Darolutamide also improved other secondary endpoints including time to castrate-resistant disease,time to pain progression,time to first skeletal-related event,and subsequent initiation of therapy.ARASENS,therefore,shows that darolutamide,in addition to ADT and docetaxel,as a form of upfront systemic intensification of“triplet”therapy for patients deemed chemotherapy-fit,represents a new standard of care for men with metastatic hormone-sensitive or castrate-sensitive prostate cancer and is anticipated to further change the landscape of treatment for this population of patients.展开更多
Aras (situated on the frontier river) is one of Iran’s important rivers which is situated on the west North of the country. The concentration of heavy metals in this river was studied around Ardabil province during t...Aras (situated on the frontier river) is one of Iran’s important rivers which is situated on the west North of the country. The concentration of heavy metals in this river was studied around Ardabil province during the 1389 in all four seasons (spring, summer, autumn, winter). The cluster analysis technique with the help of the results gained from density of metals like (Zn, Cu, Fe, Hg, Ni, Pb, Cd) was used in the water of Aras river for classifying quality of the river. Accord- ing to the gained results from the cluster analysis, the stations were divided into three groups with high pollution (HP), medium pollution (MP) and low pollution (LP). In general, S3, S5 stations with high pollution, S2, S4 stations with medium pollution and S1 station with low pollutions are classified in the water of Aras River.展开更多
We use the High-energy Electron Experiments(HEP)instrument onboard Arase(ERG)to conduct an energy-dependent cross-satellite calibration of electron fluxes measured by the High Energy Particle Detector(HEPD)onboard Fen...We use the High-energy Electron Experiments(HEP)instrument onboard Arase(ERG)to conduct an energy-dependent cross-satellite calibration of electron fluxes measured by the High Energy Particle Detector(HEPD)onboard FengYun-4A(FY-4A)spanning from April 1,2017,to September 30,2019.By tracing the two-dimensional magnetic positions(L,magnetic local time[MLT])of FY-4A at each time,we compare the datasets of the conjugate electron fluxes over the range of 245–894 keV in 6 energy channels for the satellite pair within different sets of L×MLT.The variations in the electron fluxes observed by FY-4A generally agree with the Arase measurements,and the percentages of the ratios of electron flux conjunctions within a factor of 2 are larger than 50%.Compared with Arase,FY-4A systematically overestimates electron fluxes at all 6 energy channels,with the corresponding calibration factors ranging from 0.67 to 0.81.After the cross-satellite calibration,the electron flux conjunctions between FY-4A and Arase show better agreement,with much smaller normalized root mean square errors.Our results provide a valuable reference for the application of FY-4A high-energy electron datasets to in-depth investigations of the Earth’s radiation belt electron dynamics.展开更多
Analysis of solar-cell array panel (SAP) data from the Arase satellite orbiting in the inner magnetosphere showed a clear degradation of solar cells that could be attributed to trapped protons with energies greater th...Analysis of solar-cell array panel (SAP) data from the Arase satellite orbiting in the inner magnetosphere showed a clear degradation of solar cells that could be attributed to trapped protons with energies greater than 6 MeV. Proton fluence was determined based on variations in the open-circuit voltage (Voc) of the solar cells, which we compared with that expected based on various distribution models (AP8MAX, AP9 mean and CRRESPRO quiet) of trapped protons. We found a general agreement, confirming the major contribution of trapped protons to the degradation, as well as a slight difference in the fluence expected based on the model calculations. To minimize this difference, we slightly modified the models, and found that concentrating the energetic protons on the magnetic equator provided a better agreement. Our results indicate that >6 MeV protons also has the equatorial concentration as reported for >18 MeV protons from the Van Allen Probes observation, and are interpreted as two components of the trapped protons, i.e., those of solar energetic particle (SEP) origin have an anisotropic pitch-angle distribution and are confined near the magnetic equator.展开更多
为建立花生致敏蛋白Ara h 2双抗夹心酶联免疫吸附试验(ELISA)检测方法,本研究基于所制备的抗体,采用双抗体夹心检测模式,以Ara h 2鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,并通过棋盘法优化抗体工作浓度,对该方法的灵...为建立花生致敏蛋白Ara h 2双抗夹心酶联免疫吸附试验(ELISA)检测方法,本研究基于所制备的抗体,采用双抗体夹心检测模式,以Ara h 2鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,并通过棋盘法优化抗体工作浓度,对该方法的灵敏度、准确度、精密度和特异性进行鉴定。结果表明,建立的双抗夹心ELISA检测方法对Ara h 2的检出限为5.04 ng·mL^(-1),线性范围为15.63~1 000 ng·mL^(-1),添加回收率为80.12%~96.03%,批内和批间变异系数均小于10%,且特异性良好、与其他常见食物过敏原无交叉反应。本研究可为致敏蛋白Ara h 2检测提供一种快速高效的方法。展开更多
花生过敏蛋白Ara h 3广泛存在于各类花生制品中,少量摄入即可引发过敏反应,严重威胁花生过敏患者的健康。精准地识别Ara h 3便于开展后续免疫学检测,因此,制备高纯度、高特异性的抗体显得尤为重要。以花生脱脂粉为原料,通过HiTrap Q HP...花生过敏蛋白Ara h 3广泛存在于各类花生制品中,少量摄入即可引发过敏反应,严重威胁花生过敏患者的健康。精准地识别Ara h 3便于开展后续免疫学检测,因此,制备高纯度、高特异性的抗体显得尤为重要。以花生脱脂粉为原料,通过HiTrap Q HP阴离子交换层析柱和HiLoad Superdex 200 pg制备级分子筛层析柱,制备高纯度的花生致敏蛋白Ara h 3,将其与CNBr-activated SepharoseTM 4B进行偶联,利用不同洗脱液对血清样品中的非特异性及特异性物质进行分离,制备高纯度、高特异性的抗Ara h 3多克隆抗体。结果表明:Gly-HCl洗脱的蛋白质量浓度为0.6 mg/mL;SDS-PAGE分析显示,多克隆抗体中的非特异性物质已被有效去除;免疫印迹和ELISA试验证明,纯化后的抗体仍具有较高的特异性,其效价达到1∶51200。上述结果表明抗体成功纯化,为花生过敏原Ara h 3的检测研究奠定了基础。展开更多
旨在为预防花生过敏提供新的思路和方法,考察了不同烘烤温度、烘烤时间和样品量(平铺于4 cm×4 cm锡箔纸)对Ara h 3抗原性的影响,利用正交试验得到Ara h 3抗原性增强程度最高的烘烤处理条件,并采用双缩脲法、紫外光谱、傅里叶变换...旨在为预防花生过敏提供新的思路和方法,考察了不同烘烤温度、烘烤时间和样品量(平铺于4 cm×4 cm锡箔纸)对Ara h 3抗原性的影响,利用正交试验得到Ara h 3抗原性增强程度最高的烘烤处理条件,并采用双缩脲法、紫外光谱、傅里叶变换红外光谱和荧光光谱探究烘烤对Ara h 3溶解度、游离巯基含量、二级结构和疏水性的影响。结果表明:烘烤后Ara h 3抗原性增强,且随烘烤温度升高和烘烤时间延长Ara h 3的抗原性呈先增强后减弱的趋势,样品量在70~90 mg时抗原性相差不大;在烘烤温度140℃、烘烤时间20 min,样品量80 mg时,抗原性增强程度最高,达到34.12%;烘烤后Ara h 3溶解度和游离巯基含量显著降低,蛋白质发生解折叠,无规卷曲含量增加,蛋白质分子趋于无序状态,同时,蛋白质部分肽链舒展,疏水基团暴露,外源荧光强度增强。综上,烘烤处理可以改变Ara h 3的结构,使其抗原表位暴露或者产生新的抗原表位,显著增强Ara h 3的抗原性。花生加工过程中应规避致使花生抗原性增强的烘烤条件,以降低花生致敏性。展开更多
文摘ARASENS is an international phase III trial demonstrating the beneficial role of darolutamide,a novel antiandrogen,when added to docetaxel and androgen deprivation therapy(ADT),with improvement in overall survival in men with metastatic castration-sensitive or hormone-sensitive prostate cancer.Darolutamide is a nonsteroidal androgen receptor antagonist that has been previously shown to improve metastasis-free survival in nonmetastatic castration-resistant prostate cancer.ARASENS showed that the risk of death was 32.5%lower in those who received additional darolutamide compared to ADT and docetaxel alone.Darolutamide also improved other secondary endpoints including time to castrate-resistant disease,time to pain progression,time to first skeletal-related event,and subsequent initiation of therapy.ARASENS,therefore,shows that darolutamide,in addition to ADT and docetaxel,as a form of upfront systemic intensification of“triplet”therapy for patients deemed chemotherapy-fit,represents a new standard of care for men with metastatic hormone-sensitive or castrate-sensitive prostate cancer and is anticipated to further change the landscape of treatment for this population of patients.
文摘Aras (situated on the frontier river) is one of Iran’s important rivers which is situated on the west North of the country. The concentration of heavy metals in this river was studied around Ardabil province during the 1389 in all four seasons (spring, summer, autumn, winter). The cluster analysis technique with the help of the results gained from density of metals like (Zn, Cu, Fe, Hg, Ni, Pb, Cd) was used in the water of Aras river for classifying quality of the river. Accord- ing to the gained results from the cluster analysis, the stations were divided into three groups with high pollution (HP), medium pollution (MP) and low pollution (LP). In general, S3, S5 stations with high pollution, S2, S4 stations with medium pollution and S1 station with low pollutions are classified in the water of Aras River.
基金supported by the National Natural Science Foundation of China(Grant Nos.42025404,42188101,42241143,41931073,and 42204160)the National Key R&D Program of China(Grant Nos.2022YFF0503700,2022YFF0503900,and 2021YFA0718600)+1 种基金the B-type Strategic Priority Program of the Chinese Academy of Sciences(Grant No.XDB41000000)the Fundamental Research Funds for the Central Universities(Grant Nos.2042022kf1012 and 2042022kf1016).
文摘We use the High-energy Electron Experiments(HEP)instrument onboard Arase(ERG)to conduct an energy-dependent cross-satellite calibration of electron fluxes measured by the High Energy Particle Detector(HEPD)onboard FengYun-4A(FY-4A)spanning from April 1,2017,to September 30,2019.By tracing the two-dimensional magnetic positions(L,magnetic local time[MLT])of FY-4A at each time,we compare the datasets of the conjugate electron fluxes over the range of 245–894 keV in 6 energy channels for the satellite pair within different sets of L×MLT.The variations in the electron fluxes observed by FY-4A generally agree with the Arase measurements,and the percentages of the ratios of electron flux conjunctions within a factor of 2 are larger than 50%.Compared with Arase,FY-4A systematically overestimates electron fluxes at all 6 energy channels,with the corresponding calibration factors ranging from 0.67 to 0.81.After the cross-satellite calibration,the electron flux conjunctions between FY-4A and Arase show better agreement,with much smaller normalized root mean square errors.Our results provide a valuable reference for the application of FY-4A high-energy electron datasets to in-depth investigations of the Earth’s radiation belt electron dynamics.
文摘Analysis of solar-cell array panel (SAP) data from the Arase satellite orbiting in the inner magnetosphere showed a clear degradation of solar cells that could be attributed to trapped protons with energies greater than 6 MeV. Proton fluence was determined based on variations in the open-circuit voltage (Voc) of the solar cells, which we compared with that expected based on various distribution models (AP8MAX, AP9 mean and CRRESPRO quiet) of trapped protons. We found a general agreement, confirming the major contribution of trapped protons to the degradation, as well as a slight difference in the fluence expected based on the model calculations. To minimize this difference, we slightly modified the models, and found that concentrating the energetic protons on the magnetic equator provided a better agreement. Our results indicate that >6 MeV protons also has the equatorial concentration as reported for >18 MeV protons from the Van Allen Probes observation, and are interpreted as two components of the trapped protons, i.e., those of solar energetic particle (SEP) origin have an anisotropic pitch-angle distribution and are confined near the magnetic equator.
文摘为建立花生致敏蛋白Ara h 2双抗夹心酶联免疫吸附试验(ELISA)检测方法,本研究基于所制备的抗体,采用双抗体夹心检测模式,以Ara h 2鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,并通过棋盘法优化抗体工作浓度,对该方法的灵敏度、准确度、精密度和特异性进行鉴定。结果表明,建立的双抗夹心ELISA检测方法对Ara h 2的检出限为5.04 ng·mL^(-1),线性范围为15.63~1 000 ng·mL^(-1),添加回收率为80.12%~96.03%,批内和批间变异系数均小于10%,且特异性良好、与其他常见食物过敏原无交叉反应。本研究可为致敏蛋白Ara h 2检测提供一种快速高效的方法。
文摘花生过敏蛋白Ara h 3广泛存在于各类花生制品中,少量摄入即可引发过敏反应,严重威胁花生过敏患者的健康。精准地识别Ara h 3便于开展后续免疫学检测,因此,制备高纯度、高特异性的抗体显得尤为重要。以花生脱脂粉为原料,通过HiTrap Q HP阴离子交换层析柱和HiLoad Superdex 200 pg制备级分子筛层析柱,制备高纯度的花生致敏蛋白Ara h 3,将其与CNBr-activated SepharoseTM 4B进行偶联,利用不同洗脱液对血清样品中的非特异性及特异性物质进行分离,制备高纯度、高特异性的抗Ara h 3多克隆抗体。结果表明:Gly-HCl洗脱的蛋白质量浓度为0.6 mg/mL;SDS-PAGE分析显示,多克隆抗体中的非特异性物质已被有效去除;免疫印迹和ELISA试验证明,纯化后的抗体仍具有较高的特异性,其效价达到1∶51200。上述结果表明抗体成功纯化,为花生过敏原Ara h 3的检测研究奠定了基础。
文摘旨在为预防花生过敏提供新的思路和方法,考察了不同烘烤温度、烘烤时间和样品量(平铺于4 cm×4 cm锡箔纸)对Ara h 3抗原性的影响,利用正交试验得到Ara h 3抗原性增强程度最高的烘烤处理条件,并采用双缩脲法、紫外光谱、傅里叶变换红外光谱和荧光光谱探究烘烤对Ara h 3溶解度、游离巯基含量、二级结构和疏水性的影响。结果表明:烘烤后Ara h 3抗原性增强,且随烘烤温度升高和烘烤时间延长Ara h 3的抗原性呈先增强后减弱的趋势,样品量在70~90 mg时抗原性相差不大;在烘烤温度140℃、烘烤时间20 min,样品量80 mg时,抗原性增强程度最高,达到34.12%;烘烤后Ara h 3溶解度和游离巯基含量显著降低,蛋白质发生解折叠,无规卷曲含量增加,蛋白质分子趋于无序状态,同时,蛋白质部分肽链舒展,疏水基团暴露,外源荧光强度增强。综上,烘烤处理可以改变Ara h 3的结构,使其抗原表位暴露或者产生新的抗原表位,显著增强Ara h 3的抗原性。花生加工过程中应规避致使花生抗原性增强的烘烤条件,以降低花生致敏性。
