A pot culture trial was conducted to investigate the changes of the biomass and acid phosphatase （APase） activity in 10 maize lines under low-P stress. P-deficiency significantly decreased the biomass, but induced t...A pot culture trial was conducted to investigate the changes of the biomass and acid phosphatase （APase） activity in 10 maize lines under low-P stress. P-deficiency significantly decreased the biomass, but induced the significant enhancement of the APase activity. Since P-deficiency had smaller effects on the low-P tolerant maize lines compared with P-sensitive lines, it was demonstrated that differences of tolerance to P-deficiency existed among 10 different maize lines. In addition, the relative biomass and APase activity changed during the vegetative stage of development, and there existed a significant correlation between the biomass and APase activity under low-P stress. These results suggest that the biomass and APase activity can be regarded as indicative traits of maize lines for tolerance to low-P stress at seedling stage.展开更多
APase activity is involved in regulating many physiological and developmental events by affecting the resorption process. In this study, we investigate the role of APase activity in tuber development in potato. APase ...APase activity is involved in regulating many physiological and developmental events by affecting the resorption process. In this study, we investigate the role of APase activity in tuber development in potato. APase activities were mainly localized in cytoplasm, gaps among cells and stroma of amyloplasts of parenchyma cells at the stage of tuber swelling. AP1, encoding a putative APase, was also highly expressed in swelling tubers and a low level of expression was observed in elongated stolons and matured tubers. Inhibition of APase activity by applying Brefeldin A, an inhibitor of APase production and secretion, significantly suppressed the tuber swelling and moderately affected the stolon elongation and the tuberization frequency. During tuber development, sucrose serves as the main soluble sugar for long-distance transportation and resorption. Moreover, inhibition of APase activity by Brefeldin A markedly reduced the sucrose content in tubers and further decreased the starch accumulation, suggesting that the function of APase in regulating the tuber swelling might be at least partially mediated by the sugar resorption. Exogenous sucrose treatments further indicate the important role of sucrose-mediated sugar resorption in tuber swelling. These results suggest that the APase activity might affect the tuber swelling by partially regulating the sucrose-mediated sugar resorption.展开更多
Increasing nitrogen(N)deposition exacerbates phosphorus(P)limitations in subtropical Chinese fir plantations,yet clonal mechanisms mediating root adaptation to heterogeneous P environments remain unclear.This study in...Increasing nitrogen(N)deposition exacerbates phosphorus(P)limitations in subtropical Chinese fir plantations,yet clonal mechanisms mediating root adaptation to heterogeneous P environments remain unclear.This study investigates the growth and metabolic responses of three clones(Y061/Y020:P-efficient;Y2C:P-sensitive)under N deposition and contrasting P distributions.Elevated N deposition enhanced aboveground and belowground biomass under heterogeneous P conditions,particularly enhancing Y061’s root length and surface area.Elevated N deposition significantly increased APase activity while decreasing organic acid secretion,particularly under homogeneous P-deficient conditions.Heterogeneous P supply amplified clonal divergence:P-efficient clones exhibited higher phosphorus absorption efficiency(PAE)than Y2C through root morphological plasticity,while N deposition upregulated APase activity but reduced total organic acids secretion.Metabolomic revealed N-driven shifts in exudate profiles,with lactic,malonic,succinic and oxalic acid increasing while shikimic,quinic and malic acids decreased.Notably,nitrogen absorption efficiency synergistically enhanced PAE under high N conditions.Clones Y061 and Y020 demonstrated superior N and P absorption capabilities,while clone Y2C prioritized enzymatic P mobilization in homogeneous deficiency but showed compromised growth.We demonstrate that N deposition restructures root foraging strategies along a“morphological-enzymatic”axis,where P-efficient clones exploit spatial nutrient heterogeneity through root proliferation rather than organic acid investment.These findings provide actionable solutions:(i)deploying Y061 and Y020 clones in high-N regions improves productivity and(ii)Mixed plantations mimicking heterogeneous P distribution enhance nutrient resilience.Our findings contribute to a deeper understanding of nutrient dynamics and providing targeted strategies for sustainable forestry in acidified subtropical soils.展开更多
To understand whether genotypic variation in acid phosphatase(APase)activity in rapeseed(Brassica napus L.)induced by phosphorus(P)deficiency has impact on P efficiency,soil APase activity in the rhizosphere for rapes...To understand whether genotypic variation in acid phosphatase(APase)activity in rapeseed(Brassica napus L.)induced by phosphorus(P)deficiency has impact on P efficiency,soil APase activity in the rhizosphere for rapeseed P-efficient genotype 102 and P-inefficient genotype 105 was measured against organic and inorganic P sources in the pot experiment,and the activities of root-secreted APase and leaf intracellular APase were investigated in different P-starvation periods in the nutrient solution.Higher activity of root-secreted APase in B.napus was induced under low P conditions.However,P nutrition and P uptake efficiency of the plants supplied with organic P were not directly related to the activity of root-secreted APase due to several confounding factors affecting APase availability.The higher activity of leaf APase improved P remobilization in plants and played important roles in enhancing P use efficiency,shown by the significant correlation between leaf APase activity and P use efficiency in a rapeseed recombinant inbred population of 135 lines.展开更多
基金Scientific Research Project of Chongqing Educational Commission (KJ051301)
文摘A pot culture trial was conducted to investigate the changes of the biomass and acid phosphatase （APase） activity in 10 maize lines under low-P stress. P-deficiency significantly decreased the biomass, but induced the significant enhancement of the APase activity. Since P-deficiency had smaller effects on the low-P tolerant maize lines compared with P-sensitive lines, it was demonstrated that differences of tolerance to P-deficiency existed among 10 different maize lines. In addition, the relative biomass and APase activity changed during the vegetative stage of development, and there existed a significant correlation between the biomass and APase activity under low-P stress. These results suggest that the biomass and APase activity can be regarded as indicative traits of maize lines for tolerance to low-P stress at seedling stage.
基金the Southeast University Foundation for Excellent Young Scholars (4023001013).
文摘APase activity is involved in regulating many physiological and developmental events by affecting the resorption process. In this study, we investigate the role of APase activity in tuber development in potato. APase activities were mainly localized in cytoplasm, gaps among cells and stroma of amyloplasts of parenchyma cells at the stage of tuber swelling. AP1, encoding a putative APase, was also highly expressed in swelling tubers and a low level of expression was observed in elongated stolons and matured tubers. Inhibition of APase activity by applying Brefeldin A, an inhibitor of APase production and secretion, significantly suppressed the tuber swelling and moderately affected the stolon elongation and the tuberization frequency. During tuber development, sucrose serves as the main soluble sugar for long-distance transportation and resorption. Moreover, inhibition of APase activity by Brefeldin A markedly reduced the sucrose content in tubers and further decreased the starch accumulation, suggesting that the function of APase in regulating the tuber swelling might be at least partially mediated by the sugar resorption. Exogenous sucrose treatments further indicate the important role of sucrose-mediated sugar resorption in tuber swelling. These results suggest that the APase activity might affect the tuber swelling by partially regulating the sucrose-mediated sugar resorption.
基金the Central Government Guided Local Science and Technology Development Fund(2024L3003)the National Natural Science Foundation of Fujian Province,China(2022J01122).
文摘Increasing nitrogen(N)deposition exacerbates phosphorus(P)limitations in subtropical Chinese fir plantations,yet clonal mechanisms mediating root adaptation to heterogeneous P environments remain unclear.This study investigates the growth and metabolic responses of three clones(Y061/Y020:P-efficient;Y2C:P-sensitive)under N deposition and contrasting P distributions.Elevated N deposition enhanced aboveground and belowground biomass under heterogeneous P conditions,particularly enhancing Y061’s root length and surface area.Elevated N deposition significantly increased APase activity while decreasing organic acid secretion,particularly under homogeneous P-deficient conditions.Heterogeneous P supply amplified clonal divergence:P-efficient clones exhibited higher phosphorus absorption efficiency(PAE)than Y2C through root morphological plasticity,while N deposition upregulated APase activity but reduced total organic acids secretion.Metabolomic revealed N-driven shifts in exudate profiles,with lactic,malonic,succinic and oxalic acid increasing while shikimic,quinic and malic acids decreased.Notably,nitrogen absorption efficiency synergistically enhanced PAE under high N conditions.Clones Y061 and Y020 demonstrated superior N and P absorption capabilities,while clone Y2C prioritized enzymatic P mobilization in homogeneous deficiency but showed compromised growth.We demonstrate that N deposition restructures root foraging strategies along a“morphological-enzymatic”axis,where P-efficient clones exploit spatial nutrient heterogeneity through root proliferation rather than organic acid investment.These findings provide actionable solutions:(i)deploying Y061 and Y020 clones in high-N regions improves productivity and(ii)Mixed plantations mimicking heterogeneous P distribution enhance nutrient resilience.Our findings contribute to a deeper understanding of nutrient dynamics and providing targeted strategies for sustainable forestry in acidified subtropical soils.
基金supported by the National Basic Research and Development Program of China(Grant No.2005CB120905)the National High Technology Program of China(Grant No.2006AA10A112)
文摘To understand whether genotypic variation in acid phosphatase(APase)activity in rapeseed(Brassica napus L.)induced by phosphorus(P)deficiency has impact on P efficiency,soil APase activity in the rhizosphere for rapeseed P-efficient genotype 102 and P-inefficient genotype 105 was measured against organic and inorganic P sources in the pot experiment,and the activities of root-secreted APase and leaf intracellular APase were investigated in different P-starvation periods in the nutrient solution.Higher activity of root-secreted APase in B.napus was induced under low P conditions.However,P nutrition and P uptake efficiency of the plants supplied with organic P were not directly related to the activity of root-secreted APase due to several confounding factors affecting APase availability.The higher activity of leaf APase improved P remobilization in plants and played important roles in enhancing P use efficiency,shown by the significant correlation between leaf APase activity and P use efficiency in a rapeseed recombinant inbred population of 135 lines.
