目的观察中青年高血压合并不稳定心绞痛患者动态脉压、脉压指数水平的变化,并探讨其与血清基质金属蛋白酶-9（MMP-9）、超敏C反应蛋白（Hs-CRP）水平的关系。方法选择中青年高血压合并不稳定心绞痛患者102例作为观察组,采用酶联免疫吸...目的观察中青年高血压合并不稳定心绞痛患者动态脉压、脉压指数水平的变化,并探讨其与血清基质金属蛋白酶-9（MMP-9）、超敏C反应蛋白（Hs-CRP）水平的关系。方法选择中青年高血压合并不稳定心绞痛患者102例作为观察组,采用酶联免疫吸附法（ELISA）测定MMP-9与Hs-CRP水平,选择同期健康体检者94例为对照组。将观察组按动态脉压进行分层,分为41~60 mm Hg、61~80mmHg≥81mmHg 3个水平;脉压指数进行分层,分为APPI≤0.400、0.401~0.500、≥0.501 3个水平;比较不同动态脉压和脉压指数水平时MMP-9、Hs-CRP水平的变化,并进行MMP-9与Hs-CRP之间的直线相关和回归分析。结果观察组MMP-9、Hs-CRP水平明显高于对照组;动态脉压与脉压指数水平越大,观察组血清MMP-9、Hs-CRP水平越高;直线相关和回归分析表明,MMP-9与Hs-CRP呈显著正相关。结论血清MMP-9、Hs-CRP水平升高与中青年高血压合并不稳定心绞痛密切相关;动态脉压、脉压指数与MMP-9、Hs-CRP密切相关。展开更多
In this study, the Alaska pollock protein isolate(APPI) was hydrolyzed by Neutrase for 20, 40, 80, 120, 160, 200, and 240 min. Hydrolysates with different molecular weights were produced and they were named as H1–H7....In this study, the Alaska pollock protein isolate(APPI) was hydrolyzed by Neutrase for 20, 40, 80, 120, 160, 200, and 240 min. Hydrolysates with different molecular weights were produced and they were named as H1–H7. Furthermore, the effects of hydrolysis on the average molecular weights, functional properties(solubility, oil-holding capacities, foaming activities, and emulsifying properties), and antioxidant activities(1, 1-diphenyl-2-picrylhydrazyl, superoxide, and hydroxyl free radical-scavenging activities) were determined. It was found that when the degree of hydrolysis(DH) increased, the average molecular weights of the hydrolysates decreased significantly. The functional properties of APPI were also significantly improved. The hydrolysates of APPI exhibited better solubility, emulsifying activities, and foaming activities. Hydrolysates with low molecular weights(<1 kDa) had better solubility, oil-holding capacities, and emulsifying activities, while hydrolysates with higher molecular weights(>1 kDa) had better foaming activities. In addition, the hydrolysates exhibited excellent antioxidant properties, while the inhibition values of 1, 1-diphenyl-2-picryl hydroxyl(DPPH), superoxide, and hydroxyl free radical-scavenging activities, were 85.22%, 53.56%, and 75.00% respectively, when the concentration of the hydrolysates was 5.0 mg mL^(-1). The lower the average molecular weight was, the higher was the antioxidant activity. These results indicated that hydrolysis with Neutrase is an effective method for improving the functional and antioxidant properties of APPI. The hydrolysates of APPI displayed great potentials to be used as natural antioxidants in protein-rich aqueous foods such as nutrient supplements and sports beverages.展开更多
Dissolved organic matter(DOM)in refinery wastewater is an extremely complex mixture of various organic compounds.Using mass spectrometry,it is impossible to characterize all of the DOM molecules with only one ionizati...Dissolved organic matter(DOM)in refinery wastewater is an extremely complex mixture of various organic compounds.Using mass spectrometry,it is impossible to characterize all of the DOM molecules with only one ionization source.In this study,negative-ion,electrospray ionization(ESI),positive-ion ESI,and positive-ion atmospheric pressure photoionization(APPI)were coupled with Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR MS)to analyze the molecular composition of DOM in a refinery wastewater stream during the treatment process.There were obvious differences in the heteroatom composition,number of DOM constituents,and chemical properties in refinery wastewater under the three ionization modes.Acidic CHO and CHOS compounds detected by(+)ESI,basic CHN and CHON compounds detected by(þ)ESI,and hydrocarbons detected by(+)APPI were analyzed to determine the molecular transformations that occurred during treatment.In an anaerobic biological treatment process,acidic CHO and CHOS compounds with a high oxygen content were preferentially removed,and acidic CHO and CHOS compounds with a low oxygen content were produced.In an aerobic biological process,acidic CHO and CHOS compounds with a low oxygen content were preferentially removed,and acidic CHO and CHOS compounds with a high oxygen content were produced.The whole biological treatment process has a poor removal efficiency for CHN and CHON compounds,and hydrocarbons.An activated carbon(AC)adsorption process removed different heteroatom compounds mainly with a low oxygen content for acidic and basic compounds.The transformation mechanism of CHO and CHOS compounds in the biological treatment process was analyzed by the Kendrick mass defect(KMD)theory and a mass difference network analysis.In the anaerobic process,large amounts of oxygenated CHO and CHOS compounds were degraded by decarboxylation,deoxydation,demethoxylation,and dehydration reactions,and converted to lower oxygen content compounds.In the aerobic processes,these low oxygen CHO and CHOS compounds mainly underwent carboxylation and oxidation reactions.This study determined the transformation characteristics and mechanisms of different types of organic compounds in refinery wastewater during the treatment process.The results provide guidance for the design and optimization of technologies for refinery wastewater treatment.展开更多
In this paper orthogonal matrix polynomials with respect to a right matrix moment functional an introduced. Basic results, important examples and applications to the approximation of matrix integrals are studied. Erro...In this paper orthogonal matrix polynomials with respect to a right matrix moment functional an introduced. Basic results, important examples and applications to the approximation of matrix integrals are studied. Error bounds for the proposed matrix quadrature rules are given.展开更多
文摘目的观察中青年高血压合并不稳定心绞痛患者动态脉压、脉压指数水平的变化,并探讨其与血清基质金属蛋白酶-9（MMP-9）、超敏C反应蛋白（Hs-CRP）水平的关系。方法选择中青年高血压合并不稳定心绞痛患者102例作为观察组,采用酶联免疫吸附法（ELISA）测定MMP-9与Hs-CRP水平,选择同期健康体检者94例为对照组。将观察组按动态脉压进行分层,分为41~60 mm Hg、61~80mmHg≥81mmHg 3个水平;脉压指数进行分层,分为APPI≤0.400、0.401~0.500、≥0.501 3个水平;比较不同动态脉压和脉压指数水平时MMP-9、Hs-CRP水平的变化,并进行MMP-9与Hs-CRP之间的直线相关和回归分析。结果观察组MMP-9、Hs-CRP水平明显高于对照组;动态脉压与脉压指数水平越大,观察组血清MMP-9、Hs-CRP水平越高;直线相关和回归分析表明,MMP-9与Hs-CRP呈显著正相关。结论血清MMP-9、Hs-CRP水平升高与中青年高血压合并不稳定心绞痛密切相关;动态脉压、脉压指数与MMP-9、Hs-CRP密切相关。
基金supported by grants from the China Postdoctoral Science Foundation to Dr.Chuyi Liu(No.2016M592251)
文摘In this study, the Alaska pollock protein isolate(APPI) was hydrolyzed by Neutrase for 20, 40, 80, 120, 160, 200, and 240 min. Hydrolysates with different molecular weights were produced and they were named as H1–H7. Furthermore, the effects of hydrolysis on the average molecular weights, functional properties(solubility, oil-holding capacities, foaming activities, and emulsifying properties), and antioxidant activities(1, 1-diphenyl-2-picrylhydrazyl, superoxide, and hydroxyl free radical-scavenging activities) were determined. It was found that when the degree of hydrolysis(DH) increased, the average molecular weights of the hydrolysates decreased significantly. The functional properties of APPI were also significantly improved. The hydrolysates of APPI exhibited better solubility, emulsifying activities, and foaming activities. Hydrolysates with low molecular weights(<1 kDa) had better solubility, oil-holding capacities, and emulsifying activities, while hydrolysates with higher molecular weights(>1 kDa) had better foaming activities. In addition, the hydrolysates exhibited excellent antioxidant properties, while the inhibition values of 1, 1-diphenyl-2-picryl hydroxyl(DPPH), superoxide, and hydroxyl free radical-scavenging activities, were 85.22%, 53.56%, and 75.00% respectively, when the concentration of the hydrolysates was 5.0 mg mL^(-1). The lower the average molecular weight was, the higher was the antioxidant activity. These results indicated that hydrolysis with Neutrase is an effective method for improving the functional and antioxidant properties of APPI. The hydrolysates of APPI displayed great potentials to be used as natural antioxidants in protein-rich aqueous foods such as nutrient supplements and sports beverages.
基金This work was supported by the National Key Research and Development Program of China(2018YFA0605800 and 2020YFA0607600)the National Natural Science Foundation of China(42003059)Science Foundation of China University of Petroleum,Beijing(No.2462021XKBH005).
文摘Dissolved organic matter(DOM)in refinery wastewater is an extremely complex mixture of various organic compounds.Using mass spectrometry,it is impossible to characterize all of the DOM molecules with only one ionization source.In this study,negative-ion,electrospray ionization(ESI),positive-ion ESI,and positive-ion atmospheric pressure photoionization(APPI)were coupled with Fourier transform ion cyclotron resonance mass spectrometry(FT-ICR MS)to analyze the molecular composition of DOM in a refinery wastewater stream during the treatment process.There were obvious differences in the heteroatom composition,number of DOM constituents,and chemical properties in refinery wastewater under the three ionization modes.Acidic CHO and CHOS compounds detected by(+)ESI,basic CHN and CHON compounds detected by(þ)ESI,and hydrocarbons detected by(+)APPI were analyzed to determine the molecular transformations that occurred during treatment.In an anaerobic biological treatment process,acidic CHO and CHOS compounds with a high oxygen content were preferentially removed,and acidic CHO and CHOS compounds with a low oxygen content were produced.In an aerobic biological process,acidic CHO and CHOS compounds with a low oxygen content were preferentially removed,and acidic CHO and CHOS compounds with a high oxygen content were produced.The whole biological treatment process has a poor removal efficiency for CHN and CHON compounds,and hydrocarbons.An activated carbon(AC)adsorption process removed different heteroatom compounds mainly with a low oxygen content for acidic and basic compounds.The transformation mechanism of CHO and CHOS compounds in the biological treatment process was analyzed by the Kendrick mass defect(KMD)theory and a mass difference network analysis.In the anaerobic process,large amounts of oxygenated CHO and CHOS compounds were degraded by decarboxylation,deoxydation,demethoxylation,and dehydration reactions,and converted to lower oxygen content compounds.In the aerobic processes,these low oxygen CHO and CHOS compounds mainly underwent carboxylation and oxidation reactions.This study determined the transformation characteristics and mechanisms of different types of organic compounds in refinery wastewater during the treatment process.The results provide guidance for the design and optimization of technologies for refinery wastewater treatment.
文摘In this paper orthogonal matrix polynomials with respect to a right matrix moment functional an introduced. Basic results, important examples and applications to the approximation of matrix integrals are studied. Error bounds for the proposed matrix quadrature rules are given.
