OBJECTIVE To investigate the therapeutic effect of scutellarin on colitis-associated cancer(CAC)and its underlying mechanism based on Wnt/β-catenin signaling pathway.METHODS The mouse model of CAC was established by ...OBJECTIVE To investigate the therapeutic effect of scutellarin on colitis-associated cancer(CAC)and its underlying mechanism based on Wnt/β-catenin signaling pathway.METHODS The mouse model of CAC was established by azomethane oxide(AOM)and sodium dextran sulfate(DSS),followed by scutellarin treatment,with recording the body weight,diarrhea and hematochezia.After sacrificing the mice,the colorectal length and colorectal tumor were assessed.The levels of pro-inflammatory factors TNF-αand IL-6 in mice′s sera were measured by the enzyme-linked immunosorbent assay(ELISA).The colorectal lesions were appraised by hematoxylin and eosin(H&E)staining.Theβ-catenin level in CAC tissues was probed by immunofluorescent analysis.The apoptosis-related genes Bax and Bcl-2,and Wnt signaling pathway-related genesβ-catenin,GSK-3β,TCF4,c-Myc and cyclin D1 were detected by real-time quantitative RT-PCR(RT-qPCR).Finally,Western blotting analysis(WB)was employed to examine the expressions of the apoptosis and Wnt signaling pathway-related proteins.RESULTS Scutellarin significantly improved AOM/DSS-caused weight loss,colorectal length shortening,and tumor growth in mice(P<0.01).Meanwhile,colorectal lesions could be substantially alleviated by scutellarin.ELISA results showed that the levels of pro-inflammatory factors TNF-αand IL-6 were drastically lessened(P<0.01).Scutellarin also sharply inhibited the nuclear translocation ofβ-catenin,as evidenced by the reduction in the nuclear level ofβ-catenin protein.In addition,scutellarin attenuated the mRNA expression of Wnt signaling pathway-relatedβ-catenin,TCF4,c-Myc and cyclin D1,whereas it heightened GSK-3βmRNA level.These results were consolidated by WB analysis,which indicated that scutellarin could mitigate the protein levels of phospho-GSK-3β,β-catenin,TCF4,c-Myc and cyclin D1,with the increase in GSK-3βprotein in CAC tissue.Moreover,scutellarin could induce the apoptosis of CAC,demonstrated by enhanced expression of Bax and diminished expression of Bcl-2 in both mRNA and protein levels.CONCLUSION Scutellarin may ameliorate colitis-associated colorectal cancer by weakening Wnt/β-catenin signaling cascade.展开更多
How to manage and use models in DSS is a most important subject. Generally, it costs a lot of money and time to develop the model base management system in the development of DSS and most are simple in function or can...How to manage and use models in DSS is a most important subject. Generally, it costs a lot of money and time to develop the model base management system in the development of DSS and most are simple in function or cannot be used efficiently in practice. It is a very effective, applicable, and economical choice to make use of the interfaces of professional computer software to develop a model base management system. This paper presents the method of using MATLAB, a well-known statistics software, as the development platform of a model base management system. The main functional framework of a MATLAB-based model base management system is discussed. Finally, in this paper, its feasible application is illustrated in the field of construction projects.展开更多
This paper introduces the Advanced Observer Model (AOM), a novel framework that integrates classical mechanics, quantum mechanics, and relativity through the observer’s role in constructing reality. Central to the AO...This paper introduces the Advanced Observer Model (AOM), a novel framework that integrates classical mechanics, quantum mechanics, and relativity through the observer’s role in constructing reality. Central to the AOM is the Static Configuration/Dynamic Configuration (SC/DC) conjugate, which examines physical systems through the interaction between static spatial configurations and dynamic quantum states. The model introduces a Constant Frame Rate (CFR) to quantize time perception, providing a discrete model for time evolution in quantum systems. By modifying the Schrödinger equation with CFR, the AOM bridges quantum and classical physics, offering a unified interpretation where classical determinism and quantum uncertainty coexist. A key feature of the AOM is its energy scaling model, where energy grows exponentially with spatial dimensionality, following the relationshipE∝(π)n. This dimensional scaling connects the discrete time perception of the observer with both quantum and classical energy distributions, providing insights into the nature of higher-dimensional spaces. Additionally, the AOM posits that spacetime curvature arises from quantum interactions, shaped by the observer’s discrete time perception. The model emphasizes the observer’s consciousness as a co-creator of reality, offering new approaches to understanding the quantum-classical transition. While speculative, the AOM opens new avenues for addressing foundational questions in quantum mechanics, relativity, dimensionality, and the nature of reality.展开更多
决策支持系统(Decision Support System,DSS)是当今管理决策领域中的一类重要的计算机应用系统,其宗旨是辅助决策者解决半结构化和非结构化问题,从而提高其决策的质量。实践证明,单纯依靠结构化程序设计方法或原型法进行系统的分析与设...决策支持系统(Decision Support System,DSS)是当今管理决策领域中的一类重要的计算机应用系统,其宗旨是辅助决策者解决半结构化和非结构化问题,从而提高其决策的质量。实践证明,单纯依靠结构化程序设计方法或原型法进行系统的分析与设计均难以适应DSS开发的需要。本文在实际工作的基础上将上述两种方法思想结合起来,且充分考虑了DSS的特点,提出了一种“需求——供给——生产”模型。它是一种多级嵌套模型,由外向里逐层深入则体现了系统分析与设计所应遵循的步骤序列。本文详细讨论了该模型各层的技术思想。展开更多
Based on the target analysis of the operation optimization for power plants, a novel system scheme called operation optimization decision support system (OODSS) is brought forward. According to the structure and desig...Based on the target analysis of the operation optimization for power plants, a novel system scheme called operation optimization decision support system (OODSS) is brought forward. According to the structure and design thinking of decision support system (DSS), the overall structure of the OODSS is studied, and the scheme of the sub systems in the OODSS such as the user interface system, the problem processing system, the database system, the model base system, the expert system (ES) and the data mining sy...展开更多
基金National Natural Science Foundation of China(81573813,81173598)Sichuan Provincial Admin⁃istration of Traditional Chinese Medicine of China(2021MS447)+1 种基金the Excellent Talent Program of Chengdu University of Traditional Chinese Medicine of China(YXRC2019002,ZRYY1917)Open Research Fund of the State Key Labora⁃tory of Southwestern Chinese Medicine Resources of China(2020XSGG006)。
文摘OBJECTIVE To investigate the therapeutic effect of scutellarin on colitis-associated cancer(CAC)and its underlying mechanism based on Wnt/β-catenin signaling pathway.METHODS The mouse model of CAC was established by azomethane oxide(AOM)and sodium dextran sulfate(DSS),followed by scutellarin treatment,with recording the body weight,diarrhea and hematochezia.After sacrificing the mice,the colorectal length and colorectal tumor were assessed.The levels of pro-inflammatory factors TNF-αand IL-6 in mice′s sera were measured by the enzyme-linked immunosorbent assay(ELISA).The colorectal lesions were appraised by hematoxylin and eosin(H&E)staining.Theβ-catenin level in CAC tissues was probed by immunofluorescent analysis.The apoptosis-related genes Bax and Bcl-2,and Wnt signaling pathway-related genesβ-catenin,GSK-3β,TCF4,c-Myc and cyclin D1 were detected by real-time quantitative RT-PCR(RT-qPCR).Finally,Western blotting analysis(WB)was employed to examine the expressions of the apoptosis and Wnt signaling pathway-related proteins.RESULTS Scutellarin significantly improved AOM/DSS-caused weight loss,colorectal length shortening,and tumor growth in mice(P<0.01).Meanwhile,colorectal lesions could be substantially alleviated by scutellarin.ELISA results showed that the levels of pro-inflammatory factors TNF-αand IL-6 were drastically lessened(P<0.01).Scutellarin also sharply inhibited the nuclear translocation ofβ-catenin,as evidenced by the reduction in the nuclear level ofβ-catenin protein.In addition,scutellarin attenuated the mRNA expression of Wnt signaling pathway-relatedβ-catenin,TCF4,c-Myc and cyclin D1,whereas it heightened GSK-3βmRNA level.These results were consolidated by WB analysis,which indicated that scutellarin could mitigate the protein levels of phospho-GSK-3β,β-catenin,TCF4,c-Myc and cyclin D1,with the increase in GSK-3βprotein in CAC tissue.Moreover,scutellarin could induce the apoptosis of CAC,demonstrated by enhanced expression of Bax and diminished expression of Bcl-2 in both mRNA and protein levels.CONCLUSION Scutellarin may ameliorate colitis-associated colorectal cancer by weakening Wnt/β-catenin signaling cascade.
文摘How to manage and use models in DSS is a most important subject. Generally, it costs a lot of money and time to develop the model base management system in the development of DSS and most are simple in function or cannot be used efficiently in practice. It is a very effective, applicable, and economical choice to make use of the interfaces of professional computer software to develop a model base management system. This paper presents the method of using MATLAB, a well-known statistics software, as the development platform of a model base management system. The main functional framework of a MATLAB-based model base management system is discussed. Finally, in this paper, its feasible application is illustrated in the field of construction projects.
文摘This paper introduces the Advanced Observer Model (AOM), a novel framework that integrates classical mechanics, quantum mechanics, and relativity through the observer’s role in constructing reality. Central to the AOM is the Static Configuration/Dynamic Configuration (SC/DC) conjugate, which examines physical systems through the interaction between static spatial configurations and dynamic quantum states. The model introduces a Constant Frame Rate (CFR) to quantize time perception, providing a discrete model for time evolution in quantum systems. By modifying the Schrödinger equation with CFR, the AOM bridges quantum and classical physics, offering a unified interpretation where classical determinism and quantum uncertainty coexist. A key feature of the AOM is its energy scaling model, where energy grows exponentially with spatial dimensionality, following the relationshipE∝(π)n. This dimensional scaling connects the discrete time perception of the observer with both quantum and classical energy distributions, providing insights into the nature of higher-dimensional spaces. Additionally, the AOM posits that spacetime curvature arises from quantum interactions, shaped by the observer’s discrete time perception. The model emphasizes the observer’s consciousness as a co-creator of reality, offering new approaches to understanding the quantum-classical transition. While speculative, the AOM opens new avenues for addressing foundational questions in quantum mechanics, relativity, dimensionality, and the nature of reality.
文摘决策支持系统(Decision Support System,DSS)是当今管理决策领域中的一类重要的计算机应用系统,其宗旨是辅助决策者解决半结构化和非结构化问题,从而提高其决策的质量。实践证明,单纯依靠结构化程序设计方法或原型法进行系统的分析与设计均难以适应DSS开发的需要。本文在实际工作的基础上将上述两种方法思想结合起来,且充分考虑了DSS的特点,提出了一种“需求——供给——生产”模型。它是一种多级嵌套模型,由外向里逐层深入则体现了系统分析与设计所应遵循的步骤序列。本文详细讨论了该模型各层的技术思想。
文摘Based on the target analysis of the operation optimization for power plants, a novel system scheme called operation optimization decision support system (OODSS) is brought forward. According to the structure and design thinking of decision support system (DSS), the overall structure of the OODSS is studied, and the scheme of the sub systems in the OODSS such as the user interface system, the problem processing system, the database system, the model base system, the expert system (ES) and the data mining sy...
