该文针对Redis数据库中两个问题,RDB(Snapshot)恢复数据不完整和AOF(Append Only File)恢复速度慢,提出了RDB+AOF的数据恢复方案。该方案借鉴检查点思想,依赖RDB和AOF两种方法,不但具有AOF恢复数据全面的特点,又兼具RDB恢复速度快的优...该文针对Redis数据库中两个问题,RDB(Snapshot)恢复数据不完整和AOF(Append Only File)恢复速度慢,提出了RDB+AOF的数据恢复方案。该方案借鉴检查点思想,依赖RDB和AOF两种方法,不但具有AOF恢复数据全面的特点,又兼具RDB恢复速度快的优势。按照此方案修改Redis源码并作对照实验,结果证明该方案可行且有效。展开更多
LSD1 （KDM1 under the new nomenclature） was the first identified lysine-specific histone demethylase belonging to the flavin-dependent amine oxidase family. Here, we report that AOF1 （KDM1B under the new nomenclatur...LSD1 （KDM1 under the new nomenclature） was the first identified lysine-specific histone demethylase belonging to the flavin-dependent amine oxidase family. Here, we report that AOF1 （KDM1B under the new nomenclature）, a mammalian protein related to LSD1, also possesses histone demethylase activity with specificity for H3K4mel and H3K4me2. Like LSD1, the highly conserved SWIRM domain is required for its enzymatic activity. However, AOF1 differs from LSD1 in several aspects. First, AOF1 does not appear to form stable protein complexes containing histone deacetylases. Second, AOF1 is found to localize to chromosomes during the mitotic phase of the cell cycle, whereas LSD1 does not. Third, AOF1 represses transcription when tethered to DNA and this repression activity is independent of its demethylase activity. Structural and functional analyses identified its unique N-terminal Zf-CW domain as essential for the demethylase activity-independent repression function. Collectively, our study identifies AOF1 as the second histone demethylase in the family of flavin-dependent amine oxidases and reveals a demethylase-independent repression function of AOF1.展开更多
Reliability is a persistent challenge in power electronics, with component failures significantly compromising system performance. Capacitors, widely used in power converters for filtering, contribute to approximately...Reliability is a persistent challenge in power electronics, with component failures significantly compromising system performance. Capacitors, widely used in power converters for filtering, contribute to approximately 30% of failures, predominantly due to electrochemical corrosion leading to capacitance degradation and catastrophic breakdowns. This paper presents a novel capacitor-free solid-state power filter(SSPF) for three-phase inverters, offering a transformative approach to mitigate reliability issues associated with conventional inductor-capacitor(LC) and active output filters(AOFs). Unlike AOFs, which depend on compact LC structures, the SSPF eliminates capacitors entirely, circumventing their inherent failure modes. Leveraging advanced solid-state devices and transformer technology, the SSPF achieves superior filtering performance, enhances system reliability, and significantly reduces component count, utilizing half the metal-oxidesemiconductor field effect transistor(MOSFET) switches required by AOFs. This design not only lowers costs but also improves efficiency. Simulation and experimental results demonstrate the SSPF's capability to deliver a sinusoidal output voltage at the fundamental frequency. These attributes position the SSPF as a robust, cost-effective, and innovative solution for modern power electronics applications.展开更多
AIM: To explore the value and significance of detecting the chlamydia trachomatis(CT) from urogenital tract samples by the acridine orange fluorescence(AOF).METHODS: 110 samples from urogenital tract were detected by ...AIM: To explore the value and significance of detecting the chlamydia trachomatis(CT) from urogenital tract samples by the acridine orange fluorescence(AOF).METHODS: 110 samples from urogenital tract were detected by AOF.RESULTS: The total positive rate is 49.0%,in which the male are 53.0%(16/30) the female are 45.0%(38/80).CONCLUSION:AOF is combination of fluorescence method and morphologic. It showes the characteristics of easiness and fastness,and can be used in screening the infection of the Chlamydia trachomatis.展开更多
文摘该文针对Redis数据库中两个问题,RDB(Snapshot)恢复数据不完整和AOF(Append Only File)恢复速度慢,提出了RDB+AOF的数据恢复方案。该方案借鉴检查点思想,依赖RDB和AOF两种方法,不但具有AOF恢复数据全面的特点,又兼具RDB恢复速度快的优势。按照此方案修改Redis源码并作对照实验,结果证明该方案可行且有效。
基金We thank Dr Ramin Shiekhattar （Wistar Institute, USA） for the baculoviruses expressing Flag-LSD1 and Drs Jianguo Song and Degui Chen （Shanghai Institute of Biochemistry and Cell Biol- ogy, China） for anti-HDAC1 antibody and H3K36me2 antibody, respectively. This study was partially supported by grants from the National Natural Science Foundation of China （90919025, 30871381）, the Ministry of Science and Technology of China （2009CB918402, 2009CB825601） and the Research Platform for Cell Signaling Networks from the Science and Technology Com- mission of Shanghai Municipality （06DZ22923）.
文摘LSD1 （KDM1 under the new nomenclature） was the first identified lysine-specific histone demethylase belonging to the flavin-dependent amine oxidase family. Here, we report that AOF1 （KDM1B under the new nomenclature）, a mammalian protein related to LSD1, also possesses histone demethylase activity with specificity for H3K4mel and H3K4me2. Like LSD1, the highly conserved SWIRM domain is required for its enzymatic activity. However, AOF1 differs from LSD1 in several aspects. First, AOF1 does not appear to form stable protein complexes containing histone deacetylases. Second, AOF1 is found to localize to chromosomes during the mitotic phase of the cell cycle, whereas LSD1 does not. Third, AOF1 represses transcription when tethered to DNA and this repression activity is independent of its demethylase activity. Structural and functional analyses identified its unique N-terminal Zf-CW domain as essential for the demethylase activity-independent repression function. Collectively, our study identifies AOF1 as the second histone demethylase in the family of flavin-dependent amine oxidases and reveals a demethylase-independent repression function of AOF1.
基金curruntly supported by the Purdue University Office of Technology Commercialization under Track Code (PRF 71167-01)。
文摘Reliability is a persistent challenge in power electronics, with component failures significantly compromising system performance. Capacitors, widely used in power converters for filtering, contribute to approximately 30% of failures, predominantly due to electrochemical corrosion leading to capacitance degradation and catastrophic breakdowns. This paper presents a novel capacitor-free solid-state power filter(SSPF) for three-phase inverters, offering a transformative approach to mitigate reliability issues associated with conventional inductor-capacitor(LC) and active output filters(AOFs). Unlike AOFs, which depend on compact LC structures, the SSPF eliminates capacitors entirely, circumventing their inherent failure modes. Leveraging advanced solid-state devices and transformer technology, the SSPF achieves superior filtering performance, enhances system reliability, and significantly reduces component count, utilizing half the metal-oxidesemiconductor field effect transistor(MOSFET) switches required by AOFs. This design not only lowers costs but also improves efficiency. Simulation and experimental results demonstrate the SSPF's capability to deliver a sinusoidal output voltage at the fundamental frequency. These attributes position the SSPF as a robust, cost-effective, and innovative solution for modern power electronics applications.
文摘AIM: To explore the value and significance of detecting the chlamydia trachomatis(CT) from urogenital tract samples by the acridine orange fluorescence(AOF).METHODS: 110 samples from urogenital tract were detected by AOF.RESULTS: The total positive rate is 49.0%,in which the male are 53.0%(16/30) the female are 45.0%(38/80).CONCLUSION:AOF is combination of fluorescence method and morphologic. It showes the characteristics of easiness and fastness,and can be used in screening the infection of the Chlamydia trachomatis.
