OBJECTIVE： To investigate the anticancer, antiin flammatory, and antiobesity activity of methanol extracts of eight distinct species： Artemisia Stolon ifera （AST）, Artemisia Selengensis （ASE）, Artemisia .la poni...OBJECTIVE： To investigate the anticancer, antiin flammatory, and antiobesity activity of methanol extracts of eight distinct species： Artemisia Stolon ifera （AST）, Artemisia Selengensis （ASE）, Artemisia .la ponica, Artemisia Montana, Artemisia Capillaris （ACA）, Artemisia Sylvatica （ASY）, Artemisio Keiskeana （AKE）, and Artemisia Scoparia （ASC） in vitro. METHODS： Antiproliferative activity was investigat ed in human breast cancer estrogen receptora pos itive T47D and negative HS578T cell lines exposed to the extracts at various concentrations （5200 mgl mL）for24, 48, and 72 h. For evaluating the antiin flammatory activity of the extracts, inhibition of ni trite synthesis was investigated in lipopolysaccha ride （LPS）stimulated cultures of macrophages cells exposed to 10, 50, 100, and 200 mglmL for 24 h. The antiobesity activity of the extracts was deter mined as triglyceride content and by a lipolysis as say in differentiated 3T3LI cells exposed to the extracts for 72 h at the same concentrations de scribed above. RESULTS： All extracts showed similar antiprolifera tive activity in a dose and timedependent man ner in HS578T cells. Although extracts at lower con centrations and shorter times stimulated growth of T47D cells, the antiproliferative effects of the extracts on T47D cells at higher concentrations （〉100 mg/ mL） for 72 h were significantly greater than those of HS578T cells. In case of antiinflammatory activi ty, some extracts （AST, ASE, ACA, and AKE） signifi cantly reduced nitric oxide production at higher concentrations in the presence of LPS compared with that in control cells. Antiobesity activity was showed with reducing lipid accumulation signifi cantly （〉50%） at concentrations above 100 mg/mL in most extracts （except AST and ACA）. Additional ly, AKE and ASC increased lipolysis by 11%24% compared with that in the control. CONCLUSION： Artemisia spp. demonstrates poten tial as bioactive food supplements.展开更多
The unusual fused b-lactone vibralactone was isolated from cultures of the basidiomycete Boreostereum vibrans and has been shown to significantly inhibit pancreatic lipase.In this study,a structure-based lead optimiza...The unusual fused b-lactone vibralactone was isolated from cultures of the basidiomycete Boreostereum vibrans and has been shown to significantly inhibit pancreatic lipase.In this study,a structure-based lead optimization of vibralactone resulted in three series of 104 analogs,among which compound C1 exhibited the most potent inhibition of pancreatic lipase,with an IC50 value of 14 nM.This activity is more than 3000-fold higher than that of vibralactone.The effect of compound C1 on obesity was investigated using high-fat diet(HFD)-induced C57BL/6 J obese mice.Treatment with compound C1 at a dose of 100 mg/kg significantly decreased HFD-induced obesity,primarily through the improvement of metabolic parameters,such as triglyceride levels.展开更多
BACKGROUND Atrial fibrillation(AF)stands as the most prevalent type of arrhythmia,affecting approximately 60 million individuals world-wide.Although antiarrhythmic drugs(AADs)remain the gold standard for AF treatment,...BACKGROUND Atrial fibrillation(AF)stands as the most prevalent type of arrhythmia,affecting approximately 60 million individuals world-wide.Although antiarrhythmic drugs(AADs)remain the gold standard for AF treatment,glucagon-like peptide-1 receptor agonists(GLP-1 RAs)are arising as potential therapeutic alternatives.AIM To evaluate the impact of GLP-1 RAs on the incidence of AF.METHODS Inclusion criteria included systematic reviews(SRs)that based their analyses on clinical trials,observational studies,controlled trials and network meta-analyses.A total of 8 SRs were selected for data extraction,focusing on semaglutide,liraglutide and dulaglutide.Additionally,the effects of GLP-1 RAs on AF incidence were compared with those of sodium-glucose co-transporter 2(SGLT2)inhibitors.RESULTS Findings indicate that semaglutide,evaluated in the largest patient cohort across the 8 SRs,consistently reduced AF incidence.However,dulaglutide and liraglutide exhibited inconsistent effects.Notably,as opposed to variable outcomes associated with GLP-1 RAs,SGLT2 inhibitors a class of antidiabetic agents with weight-reducing properties exhibit significant cardiovascular benefits,including reductions in both AF and atrial flutter.CONCLUSION GLP-1 RAs emerge as a promising and potential alternative for AADs in reduction of incidence of AF.However,further research is required to fully determine their therapeutic potential and long-term cardiovascular effects.展开更多
Objective:To evaluate the antioxidant potential and pancreatic lipase inhibitory action of optimized hydroethanolic extracts of Solanum nigrum.Methods:Optimized extraction for maximum recovery of metabolites was perfo...Objective:To evaluate the antioxidant potential and pancreatic lipase inhibitory action of optimized hydroethanolic extracts of Solanum nigrum.Methods:Optimized extraction for maximum recovery of metabolites was performed using a combination of freeze-drying and ultrasonication followed by determination of antioxidant and antiobesity properties.The ultra-high performance liquid chromatography equipped with mass spectrometry was used to analyze metabolite profiling of Solanum nigrum.Computational studies were performed using molecular docking and electrostatic potential analysis for individual compounds.The hypolipidemic potential of the most potent extract was assessed in the obese mice fed on fat rich diet.Results:The 80%hydroethanolic extract exhibited the highest extract yield,total phenolic contents,total flavonoid contents along with the strongest 2,2-diphenyl-1-picrylhydrazyl scavenging activity,total antioxidant power,and pancreatic lipase inhibitory properties.The 80%hydroethanolic extract not only regulated the lipid profile of obese mice but also restricted the weight gain in the liver,kidney,and heart.The 80%hydroethanolic extract also reduced alanine transaminase and aspartate transaminase concentrations in serum.The effects of plant extract at 300 mg/kg body weight were quite comparable with the standard drug orlistat.Conclusions:Solanum nigrum is proved as an excellent and potent source of secondary metabolites that might be responsible for obesity mitigation.展开更多
Background:Over the years,there has been research on the anti-obesity effect of the Allium cepa bulb,but a dearth of research was carried out on the leaves,which is consumed as vegetable salad and soup,hence this stud...Background:Over the years,there has been research on the anti-obesity effect of the Allium cepa bulb,but a dearth of research was carried out on the leaves,which is consumed as vegetable salad and soup,hence this study was proposed.Objective:This study aims at investigating the effect of A.cepa leaves on high fat diet(HFD)induced obesity in male Wistar rats.Methods:Twenty-eight male Wistar rats were divided into four groups with seven rats each.Apart from Group 1(normal control which received normal pelleted diet),obesity was induced in 21 rats of Group 2 to 4 with HFD.Group 2,the obese control was administered with 100%HFD,while the diet for group 3 and 4 was supplemented with 10%and 20%A.cepa powdered leaves,respectively,for 28 days.Results:In the rats treated with 10%and 20%A.cepa,body weight,fat mass,blood glucose,total cholesterol,triglycerides,aspartate amino transferase,alanine amino transferase,creatinine and urea levels were reduced significantly(P<0.05)in comparison with the obese control group.The liver of the rats treated with 10%and 20%A.cepa leaves revealed small and few amounts of fat deposits in comparison with the obese control group,which revealed numerous and large deposits of fat.The kidney of the rats treated with 10%and 20%A.cepa leaves showed moderate and mild inflammation,respectively,in comparison with the obese control group which showed acute inflammation.The leaves of A.cepa had antioxidant properties and the presence of volatile compounds with anti-obesity properties in A.cepa were identified using gas chromatography-mass spectrometry(GC-MS).Conclusion:A.cepa leaves had weight-loss effect in that it decreased body weight,fat mass,glucose and lipid levels including fat deposits in the liver.展开更多
Trehalulose(1-O-α-D-glucopyranosyl-D-fructose)is a naturally occurring disaccharide composed of fructose and glucose.It is a sucrose isomer with a uniqueα-1,1 glycosidic bond that is more stable than the 1,2 glycosi...Trehalulose(1-O-α-D-glucopyranosyl-D-fructose)is a naturally occurring disaccharide composed of fructose and glucose.It is a sucrose isomer with a uniqueα-1,1 glycosidic bond that is more stable than the 1,2 glycosidic bond found in sucrose,giving it a lower glycemic index(GI).Trehalulose sugar production is often complicated,and the literature on the production is scarce.However,trehalulose is gaining popularity after a recent study revealed the abundance of this sugar in stingless bee honey(13 to 44 g per 100 g).The current short review discusses the chemical and physiological properties of trehalulose and its potential health benefits based on a bibliometric approach.Furthermore,it evaluates the antidiabetic potential of trehalulose as an emerging alternative sweetener.展开更多
基金Supported by Priority Research Centers Program through the National Research Foundation of Korea (NRF)funded by the Ministry of Education, Science and Technology(2012-0006811)
文摘OBJECTIVE： To investigate the anticancer, antiin flammatory, and antiobesity activity of methanol extracts of eight distinct species： Artemisia Stolon ifera （AST）, Artemisia Selengensis （ASE）, Artemisia .la ponica, Artemisia Montana, Artemisia Capillaris （ACA）, Artemisia Sylvatica （ASY）, Artemisio Keiskeana （AKE）, and Artemisia Scoparia （ASC） in vitro. METHODS： Antiproliferative activity was investigat ed in human breast cancer estrogen receptora pos itive T47D and negative HS578T cell lines exposed to the extracts at various concentrations （5200 mgl mL）for24, 48, and 72 h. For evaluating the antiin flammatory activity of the extracts, inhibition of ni trite synthesis was investigated in lipopolysaccha ride （LPS）stimulated cultures of macrophages cells exposed to 10, 50, 100, and 200 mglmL for 24 h. The antiobesity activity of the extracts was deter mined as triglyceride content and by a lipolysis as say in differentiated 3T3LI cells exposed to the extracts for 72 h at the same concentrations de scribed above. RESULTS： All extracts showed similar antiprolifera tive activity in a dose and timedependent man ner in HS578T cells. Although extracts at lower con centrations and shorter times stimulated growth of T47D cells, the antiproliferative effects of the extracts on T47D cells at higher concentrations （〉100 mg/ mL） for 72 h were significantly greater than those of HS578T cells. In case of antiinflammatory activi ty, some extracts （AST, ASE, ACA, and AKE） signifi cantly reduced nitric oxide production at higher concentrations in the presence of LPS compared with that in control cells. Antiobesity activity was showed with reducing lipid accumulation signifi cantly （〉50%） at concentrations above 100 mg/mL in most extracts （except AST and ACA）. Additional ly, AKE and ASC increased lipolysis by 11%24% compared with that in the control. CONCLUSION： Artemisia spp. demonstrates poten tial as bioactive food supplements.
基金The authors are grateful to the National Natural Science Foundation of China(81102348,U1132607,81373289).
文摘The unusual fused b-lactone vibralactone was isolated from cultures of the basidiomycete Boreostereum vibrans and has been shown to significantly inhibit pancreatic lipase.In this study,a structure-based lead optimization of vibralactone resulted in three series of 104 analogs,among which compound C1 exhibited the most potent inhibition of pancreatic lipase,with an IC50 value of 14 nM.This activity is more than 3000-fold higher than that of vibralactone.The effect of compound C1 on obesity was investigated using high-fat diet(HFD)-induced C57BL/6 J obese mice.Treatment with compound C1 at a dose of 100 mg/kg significantly decreased HFD-induced obesity,primarily through the improvement of metabolic parameters,such as triglyceride levels.
文摘BACKGROUND Atrial fibrillation(AF)stands as the most prevalent type of arrhythmia,affecting approximately 60 million individuals world-wide.Although antiarrhythmic drugs(AADs)remain the gold standard for AF treatment,glucagon-like peptide-1 receptor agonists(GLP-1 RAs)are arising as potential therapeutic alternatives.AIM To evaluate the impact of GLP-1 RAs on the incidence of AF.METHODS Inclusion criteria included systematic reviews(SRs)that based their analyses on clinical trials,observational studies,controlled trials and network meta-analyses.A total of 8 SRs were selected for data extraction,focusing on semaglutide,liraglutide and dulaglutide.Additionally,the effects of GLP-1 RAs on AF incidence were compared with those of sodium-glucose co-transporter 2(SGLT2)inhibitors.RESULTS Findings indicate that semaglutide,evaluated in the largest patient cohort across the 8 SRs,consistently reduced AF incidence.However,dulaglutide and liraglutide exhibited inconsistent effects.Notably,as opposed to variable outcomes associated with GLP-1 RAs,SGLT2 inhibitors a class of antidiabetic agents with weight-reducing properties exhibit significant cardiovascular benefits,including reductions in both AF and atrial flutter.CONCLUSION GLP-1 RAs emerge as a promising and potential alternative for AADs in reduction of incidence of AF.However,further research is required to fully determine their therapeutic potential and long-term cardiovascular effects.
文摘Objective:To evaluate the antioxidant potential and pancreatic lipase inhibitory action of optimized hydroethanolic extracts of Solanum nigrum.Methods:Optimized extraction for maximum recovery of metabolites was performed using a combination of freeze-drying and ultrasonication followed by determination of antioxidant and antiobesity properties.The ultra-high performance liquid chromatography equipped with mass spectrometry was used to analyze metabolite profiling of Solanum nigrum.Computational studies were performed using molecular docking and electrostatic potential analysis for individual compounds.The hypolipidemic potential of the most potent extract was assessed in the obese mice fed on fat rich diet.Results:The 80%hydroethanolic extract exhibited the highest extract yield,total phenolic contents,total flavonoid contents along with the strongest 2,2-diphenyl-1-picrylhydrazyl scavenging activity,total antioxidant power,and pancreatic lipase inhibitory properties.The 80%hydroethanolic extract not only regulated the lipid profile of obese mice but also restricted the weight gain in the liver,kidney,and heart.The 80%hydroethanolic extract also reduced alanine transaminase and aspartate transaminase concentrations in serum.The effects of plant extract at 300 mg/kg body weight were quite comparable with the standard drug orlistat.Conclusions:Solanum nigrum is proved as an excellent and potent source of secondary metabolites that might be responsible for obesity mitigation.
文摘Background:Over the years,there has been research on the anti-obesity effect of the Allium cepa bulb,but a dearth of research was carried out on the leaves,which is consumed as vegetable salad and soup,hence this study was proposed.Objective:This study aims at investigating the effect of A.cepa leaves on high fat diet(HFD)induced obesity in male Wistar rats.Methods:Twenty-eight male Wistar rats were divided into four groups with seven rats each.Apart from Group 1(normal control which received normal pelleted diet),obesity was induced in 21 rats of Group 2 to 4 with HFD.Group 2,the obese control was administered with 100%HFD,while the diet for group 3 and 4 was supplemented with 10%and 20%A.cepa powdered leaves,respectively,for 28 days.Results:In the rats treated with 10%and 20%A.cepa,body weight,fat mass,blood glucose,total cholesterol,triglycerides,aspartate amino transferase,alanine amino transferase,creatinine and urea levels were reduced significantly(P<0.05)in comparison with the obese control group.The liver of the rats treated with 10%and 20%A.cepa leaves revealed small and few amounts of fat deposits in comparison with the obese control group,which revealed numerous and large deposits of fat.The kidney of the rats treated with 10%and 20%A.cepa leaves showed moderate and mild inflammation,respectively,in comparison with the obese control group which showed acute inflammation.The leaves of A.cepa had antioxidant properties and the presence of volatile compounds with anti-obesity properties in A.cepa were identified using gas chromatography-mass spectrometry(GC-MS).Conclusion:A.cepa leaves had weight-loss effect in that it decreased body weight,fat mass,glucose and lipid levels including fat deposits in the liver.
文摘Trehalulose(1-O-α-D-glucopyranosyl-D-fructose)is a naturally occurring disaccharide composed of fructose and glucose.It is a sucrose isomer with a uniqueα-1,1 glycosidic bond that is more stable than the 1,2 glycosidic bond found in sucrose,giving it a lower glycemic index(GI).Trehalulose sugar production is often complicated,and the literature on the production is scarce.However,trehalulose is gaining popularity after a recent study revealed the abundance of this sugar in stingless bee honey(13 to 44 g per 100 g).The current short review discusses the chemical and physiological properties of trehalulose and its potential health benefits based on a bibliometric approach.Furthermore,it evaluates the antidiabetic potential of trehalulose as an emerging alternative sweetener.
