Pu-erh tea,a traditional Chinese beverage,performs an anti-obesity function,but the correlation between its components and efficacy remains unknown.Here,we screened two Pu-erh teas with significant anti-obesity effica...Pu-erh tea,a traditional Chinese beverage,performs an anti-obesity function,but the correlation between its components and efficacy remains unknown.Here,we screened two Pu-erh teas with significant anti-obesity efficacies from 11 teas.In vitro experiments revealed that lipid accumulation in L02 cells and lipid synthesis in 3T3-L1 cells were significantly better inhibited by Tea-B than Tea-A.Further in vivo experiments using model mice revealed that the differences in chemical components generated two pathways in the anti-obesity efficacy and mechanism of Pu-erh teas.Tea-A changes the histomorphology of brown adipose tissue(BAT)and increases the abundance of Coriobacteriaceae_UCG_002 and cyclic AMP in guts through high chemical contents of cyclopentasiloxane,decamethyl,tridecane and 1,2,3-trimethoxybenzene,eventually increasing BAT activation and fat browning gene expression;the high content of hexadecane and 1,2-dimethoxybenzene in Tea-B reduces white adipose tissue(WAT)accumulation and the process of fatty liver,increases the abundance of Odoribacter and sphinganine 1-phosphate,inhibits the expression of lipid synthesis and transport genes.These mechanistic findings on the association of the representative bioactive components in Pu-erh teas with the anti-obesity phenotypes,gut microbes,gut metabolite structure and anti-obesity pathways,which were obtained for the first time,provide foundations for developing functional Pu-erh tea.展开更多
Obesity is a major driver of metabolic dysfunction-associated steatotic liver disease(MASLD)and its progressive form,metabolic dysfunction-associated steatohepatitis(MASH).As the global prevalence of obesity continues...Obesity is a major driver of metabolic dysfunction-associated steatotic liver disease(MASLD)and its progressive form,metabolic dysfunction-associated steatohepatitis(MASH).As the global prevalence of obesity continues to rise,the burden of MASLD/MASH is increasing,posing significant challenges to healthcare systems.The use of anti-obesity medications(AOMs)in this population is complex due to altered hepatic metabolism,safety concerns,and potential hepatotoxicity.Recent advances in pharmacologic agents,such as glucagon-like peptide1(GLP-1)receptor agonists(GLP-1 RAs),dual GLP-1/glucose-gastric inhibitory polypeptide(GIP)agonists,and triple GLP-1/GIP/glucagon agonists,have shown promising metabolic effects in the general population.Among these,GLP-1 RAs(e.g.,liraglutide and semaglutide)consistently demonstrate hepatic benefits,including reductions in hepatic steatosis,improvements in liver enzyme profiles,and attenuation of fibrosis progression.Tirzepatide,a dual GLP-1/GIP agonist,has shown superior weight loss effects compared to GLP-1 receptor agonist monotherapy,with emerging but still limited data on hepatic outcomes in MASLD/MASH.Retatrutide,a triple agonist,has produced the most pronounced metabolic effects to date,although its impact on liver histology remains underexplored.Other AOMs,such as bupropion-naltrexone and phentermine-topiramate,require cautious use due to potential hepatotoxicity.Importantly,advanced MASLD may alter drug pharmacokinetics,underscoring the need for individualized therapy and close monitoring.This review provides an updated synthesis of the efficacy and safety of current and emerging AOMs in patients with MASLD/MASH and highlights the urgent need for further research to define optimal pharmacological strategies in this high-risk population.展开更多
Swimming crab(Portunus trituberculatus)eggs contain abundant phospholipids and polyunsaturated fatty acids with specific bioactivities.However,more studies on the functions of other nutrients are needed.Here,a novel s...Swimming crab(Portunus trituberculatus)eggs contain abundant phospholipids and polyunsaturated fatty acids with specific bioactivities.However,more studies on the functions of other nutrients are needed.Here,a novel sialoglycopeptide from P.trituberculatus eggs,which was termed as Pt-SGP,was isolated with enzymolysis.Pt-SGP was a 6480-Da pure sialoglycopeptide.O-glycan units existed between peptides and sugars.Pt-SGP was composed of 10.69%protein,71.43%hexose,and 11.27%Neu5Gc acid.Analyzed with HPLC,GC-MS,and NMR spectrometry,the carbohydrate chain of Pt-SGP was{(4GlcNAc1-2Man1-3)/(4GlcNAc1-2Man1-6)Man1-6}/{4Glc1-4}Glc1-4Glc1-3GlcNAc1.The MS spectrometry analysis result showed that the peptide chain was Val-Ala-Thr*-Val-Ser*-Leu-Pro-Arg.The results on mice showed Pt-SGP could reduce body weight gain by 18.13%,white adipose weight by 28.57%,serum TC by 22.32,serum TG by 9.46%,serum LDL-C by 27.69%,hepatic TC by 73.21%,and hepatic TG by 25.20%.Furthernore,it could increase serum HDL-C by 75.96%,mitigate adipocyte size and hepatic lipid accumulation in high fat diet-fed mice.Pt-SGP significantly down-regulated C/EBPαsignaling and up-regulated AMPKαsignaling in the epididymal fat tissues.The findings indicate that Pt-SGP exhibits notable anti-obesity properties,and can potentially become a promising dietary supplement for anti-obesity.展开更多
Although it is not generally a life-threatening disease,obesity is becoming a major health problem worldwide.It can be controlled by means of drugs,and,consequently,these are required to be safe as well as effective.I...Although it is not generally a life-threatening disease,obesity is becoming a major health problem worldwide.It can be controlled by means of drugs,and,consequently,these are required to be safe as well as effective.In this paper,we summarize the fate of various drugs that have been introduced for clinical use in the treatment of obesity.Fenfluramine and dexfenfluramine were withdrawn because of heart valve damage.Sibutramine suppresses appetite and increases heart rate and blood pressure.In the Sibutramine Cardiovascular OUTcomes trial,an increase in major adverse cardiovascular events prompted its withdrawal in Europe and the United States.Rimonabant is an endocannabinoid receptor antagonist that reduces body weight and ameliorates some cardiovascular risk factors.However,adverse psychiatric side effects led to its withdrawal as well.Orlistat is approved in Europe and the United States for the treatment of obesity,but its use is limited by gastrointestinal side-effects.Ephedrine and caffeine are natural ingredients in foods and supplements that may help the person to lose weight.In the light of several failed attempts,there is a clear need to develop drugs that are effective and safe in the long term in order to successfully combat the phenomenon of obesity.展开更多
Pu-erh tea, a traditional Chinese beverage, has been believed to have many benefits to human health and without side effects. In this study, we systematically analyzed the main active components of Pu-erh tea and inve...Pu-erh tea, a traditional Chinese beverage, has been believed to have many benefits to human health and without side effects. In this study, we systematically analyzed the main active components of Pu-erh tea and investigated its anti-obesity, anti-atherosclerotic and anti-oxidant effects using an obese rat model. Obesity was induced by feeding a high-fat diet and subsequently the experimental obese mice were fed with high-fat diet supplemented with low (2.5%), medium (5%) or high (7.5%) doses of Pu-erh tea powder for 6 weeks respectively. As result, the body weight gain of the rats was decreased by medium and high doses of Pu-erh tea treatments. Furthermore, the levels of serum total cholesterol (TC), triglyceride (TG) and atherosclerosis index (AI) were significantly lowered by Pu-erh tea compared to the control group. Conversely, high density lipoproteincholesterol (HDL-C) level of the rats was significantly elevated by Pu-erh tea treatments. In addition, Pu-erh tea treatments increased the activities of anti-oxidative enzymes such as superoxide dismutase (SOD) and glutathione peroxides (GSH-Px), whereas reduced the level of lipid peroxidation product malondialdehyde (MDA) in obese rats. Collectively, our find-ings revealed that Pu-erh tea exerts comprehensive benefits in anti-obesity, anti-atherosclerotic and anti-oxidant effects, therefore can be used as a promising functional food in obesity management.展开更多
Mesotherapy and anti-obesity medications are gradually gaining worldwide popularity for purposes of body contouring and weight loss.Their adverse effects are various,but there is a tendency to disregard them.Ischemic ...Mesotherapy and anti-obesity medications are gradually gaining worldwide popularity for purposes of body contouring and weight loss.Their adverse effects are various,but there is a tendency to disregard them.Ischemic colitis is one of the most common diseases associated with non-obstructive blood vessel disorders.However,there have been no case reports about the adverse effects resulting from mesotherapy only or in combination with anti-obesity medications.We report on an interesting case of ischemic colitis after mesotherapy combined with anti-obesity medications in a 39-year-old female who had no risk factors.展开更多
Objective To further explore associated effects of Lactobacillus plantarum dy-1(LFBE) on obesity and lipid metabolism at the gene expression level, the expression of micro RNAs(mi RNAs) was investigated in the liver o...Objective To further explore associated effects of Lactobacillus plantarum dy-1(LFBE) on obesity and lipid metabolism at the gene expression level, the expression of micro RNAs(mi RNAs) was investigated in the liver of high-fat diet(HFD) induced obese rats.Methods Three groups of animal models were established. Changes in mi RNA expression in the liver of each group were analyzed by microarray and RT-q PCR, complemented by bioinformatics. Palmitateinduced hepatocellular carcinoma(Hep G2) cells were used as a model to validate the test.Results LFBE treatment groups and HFD groups were observed to be distinctly different with respect to rates of increase in body weight and body fat percentage and triglyceride(TG) and total cholesterol(TC) levels in serum and liver. In addition, the LFBE group showed upregulation of ten mi RNAs and downregulation of five mi RNAs in the liver. Downregulation of mi R-34 a and mi R-212 was observed in the livers of the LFBE group. Gene ontology and kyoto encyelopedia of geues and genomes(KEGG)pathway analysis showed that possible target genes of the deregulated mi RNAs were significantly enriched in the adrenergic and HIF-1 signaling pathways.Conclusion These results demonstrate that LFBE might regulate the expression of mi RNAs in order to inhibit obesity and fatty liver.展开更多
Semicarbazide-sensitive the metabolism of glucose and fat, amine oxidase （SSAO） has been considered to be associated with and elevated SSAO activity was observed in obese patients. In the present study, an in vitro ...Semicarbazide-sensitive the metabolism of glucose and fat, amine oxidase （SSAO） has been considered to be associated with and elevated SSAO activity was observed in obese patients. In the present study, an in vitro SSAO activity-based method was developed to screen inhibitors from 15 an- ti-obesity drugs. Among the fifteen anti-obesity drugs, four drugs including caffeine, fenfluramine, bumetanide and amfebutamone inhibited SSAO activity, and caffeine was the most effective one. When the concentration of caffeine was 1.4 mmol/L, the inhibition ratio was 31.9% and 18.8% in rabbit serum and rat adipose tissue, respectively. Inhibition of SSAO activity by caffeine was also confirmed in the in vivo study, showing the inhibition ratio of 15.6% on serum SSAO. Caffeine pro- vides a natural source of inhibition of SSAO activity and may be a promising inhibitor for the study of SSAO.展开更多
As a dark tea,Pu-erh tea(PET)is produced from sun-dried leaves of Camellia sinensis var.assamica mainly in Yunnan Province of China.Many microorganisms are involved in the fermentation of PET.Among them,Aspergillus ni...As a dark tea,Pu-erh tea(PET)is produced from sun-dried leaves of Camellia sinensis var.assamica mainly in Yunnan Province of China.Many microorganisms are involved in the fermentation of PET.Among them,Aspergillus niger is most important.It is believed that the longer the preservation period,the better is the quality and taste of PET,which is commercially available as loose,compressed or instant tea leaves.Chemical components of PET include flavones,flavanols,flavonols,phenolic acids,alkaloids and methylxanthines.In this overview,the lipid-lowering and anti-obesity effects of PET were discussed based on animal models and human trials,and our study provided some insights into possible mechanisms of bioactive compounds,such as theabrownin,catechins,lovastatin and gallic acid.Other bioactivities of PET and some information on Fuzhuan brick tea were also included.Sources of information cited were from Google Scholar,Pub Med,Pub Med Central,Science Direct,J-Stage,Pub Chem,Directory of Open Access Journals(DOAJ),and China National Knowledge Infrastructure(CNKI).展开更多
This study investigated hypolipidemic,weight-reducing,and hepatoprotective effects of Antrodia cinnamomea mycelial extract obtained from solid-state culture(ACME)in an HFr D-induced obese/hyperlipidemic mouse model.Fo...This study investigated hypolipidemic,weight-reducing,and hepatoprotective effects of Antrodia cinnamomea mycelial extract obtained from solid-state culture(ACME)in an HFr D-induced obese/hyperlipidemic mouse model.Following 4-week ACME treatment,body weight,epididymal fat index,and some serum biochemical indices were measured.Expression levels of some related genes involved in cholesterol and lipid metabolism were analyzed by q RT-PCR.Moreover,histological studies of hepatic tissues were also conducted.After ACME treatment,body weight and epididymal fat index were significantly lower than that in model control group.ACME and simvastatin significantly reduced serum total cholesterol(T-CHO)and low-density lipoprotein cholesterol(LDL-C)levels,and increased high-density lipoprotein cholesterol(HDL-C)level.Subsequent experiments showed that:(i)ACME regulated transcriptional expression of 3-hydroxy-3-methylglutaryl-Co A reductase(HMGR),low-density lipoprotein receptor(LDLR),adipose triglyceride lipase(ATGL),and fatty acid synthase(FAS),with consequent reduction of blood lipid levels and body weight;(ii)ACME enhanced total antioxidant capacity(T-AOC)and superoxide dismutase(SOD)activity in hepatic tissues;(iii)ACME reduced malondialdehyde(MDA)level and ameliorated lipid oxidative damage in liver.Our findings indicate that ACME is a strong candidate for development as a novel anti-hyperlipidemia and anti-obesity health product.展开更多
The prevalence of obesity has increased and is a health concern worldwide.Due to the concerns regarding synthetic anti-obesity treatments,nowadays natural products become a trend.Previous studies proved that there is ...The prevalence of obesity has increased and is a health concern worldwide.Due to the concerns regarding synthetic anti-obesity treatments,nowadays natural products become a trend.Previous studies proved that there is a potential to use marine algae as anti-obesity agents.Therefore,in this study,the lipid inhibitory effect of crude polysaccharide of amyloglucosidase-assisted hydrolysate from Sargassum thunbergii(STAC)and its fucoidan fractions(STAFs)on 3T3-L1 cells and high-fat diet(HFD)-induced obese mice were investigated.According to the results,the STAF3,showed the highest xylose content and exhibited significant inhibitory effects on lipid accumulation by downregulating adipogenic and lipogenic proteins in 3T3-L1 cells.Furthermore,oral supplementation with STAC significantly declined gain in body weight and fat weight,and serum lipid contents in an HFD-induced obesity mouse model.Structural and chemical characterizations demonstrated that puritied STAF3 has consistent surface morphology and small particle size,with similar structural characteristics as commercial fucoidan.Together,these results indicate that STAC and purified STAF3 from Sargassum thunbergia is a potent source to develop as ananti-obesity agents or functional food products to counter obesity.展开更多
The worldwide obesity epidemic has imposed human being a huge burden and attention had been drawn to manipulating the lactic acid bacteria (LAB) to improve obesity symptoms.In this study,a more comprehensive and relia...The worldwide obesity epidemic has imposed human being a huge burden and attention had been drawn to manipulating the lactic acid bacteria (LAB) to improve obesity symptoms.In this study,a more comprehensive and reliable in vitro method was established to screen LAB with greater potential against obesity.Firstly,twenty-eight human feces-derived LAB strains were selected by assessing bile salt-acid tolerance and cholesterol lowering capacity.Subsequently,the triglyceride lowering capacity,bile salt hydrolase (BSH) activity,α-glucosidase activity inhibition capacity and Enterobacter cloacae (E.cloacae) growth inhibition capacity were tested for further evaluating the anti-obesity properties of LAB strains.Principal component analysis (PCA) of these results indicated that Limosilactobacillus reuteri (L.reuteri) NCUH003,Lactiplantibacillus plantarum (L.plantarum) NCUH046 and Limosilactobacillus fermentum (L.fermentum) NCUH068 exhibited favorable anti-obesity properties.Besides,the LAB displayed good gastrointestinal adaptability and antioxidant activity but no hemolytic activity,and they were sensitive to various antibiotics.These results indicated that L.reuteri NCUH003,L.plantarum NCUH046 and L.fermentum NCUH068 are potential for application in obesity treatment.展开更多
This study aimed to elucidate the inhibition mechanism of apigenin against porcine pancreatic lipase(PPL),and,moreover,to comprehensively reveal the molecular basis of its anti-obesity via network pharmacology approac...This study aimed to elucidate the inhibition mechanism of apigenin against porcine pancreatic lipase(PPL),and,moreover,to comprehensively reveal the molecular basis of its anti-obesity via network pharmacology approach.The results showed that apigenin inhibited PPL with an IC50 value of 0.377±0.041 mM.Spectroscopic techniques combined molecular docking suggested that apigenin could bind into the PPL active pocket,affecting its normal spatial conformation.Moreover,molecular dynamic(MD)simulations revealed that the open conformation of PPL tended to transit to the closed in the presence of apigenin,which might one important reason for the inhibition of PPL catalytic ability.Network pharmacology analysis revealed that a total of 49 proteins could be identified as potential targets for the anti-obesity effects of apigenin.According to the protein-protein interaction(PPI)network analysis,six hub targets were extracted including IGF1,ESR1,MMP9,PPARA,MAPK14 and NR3C1.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment indicated that the 49 potential targets could be mapped to 30 pathways(p<0.05).Among them,PI3K-Akt signaling pathway and insulin resistance can be considered as two major pathways regulated by apigenin.Further docking studies indicated that apigenin can bind into the binding pocket of the six hub target proteins identified according to the PPI network.The results indicated that in addition to inhibiting PPL,apigenin could exhibit anti-obesity benefit through the molecular mechanisms uncovered by network pharmacology.This study proposes a new strategy to reveal the mechanisms of dietary polyphenols at the level of network pharmacology.展开更多
Objective:To investigate antioxidant and anti-obesity activity of flavonoids extracted by solvent cold percolation and preparative thin liquid chromatography from Dioscorea steriscus tubers.Methods:1-diphenyl-2-picryl...Objective:To investigate antioxidant and anti-obesity activity of flavonoids extracted by solvent cold percolation and preparative thin liquid chromatography from Dioscorea steriscus tubers.Methods:1-diphenyl-2-picrylhydrazyl(DPPH)antiradical activity was employed to investigate antioxidant activity while chromogenic method was used to determine alpha amylase inhibition activity and spectrophotometric methods using triolein as a substrate was used to investigate lipase activity.Results:Thin liquid chromatography profiling revealed eight different flavonoid types.Ethyl acetate extract yielded two types,R_(f) values 0.38 and 0.40;chloroform extract also yielded two types R_(f) values 0.06 and 0.51,while ethanol extract yielded four types with R_(f) values 0.16,0.33,0.65 and 0.96.All the extracted flavonoids exhibited antioxidant activity with ethanol extracts exhibiting the greatest antiradical activity.The order of enzyme inhibition capacity was ethyl acetate<chloroform<ethanol.Ethanol extract exhibited significantly greater anti-obesity activity as compared to herbex,a commercially anti-obesity medication sold in drug stores.Anti-α amylase activity and anti-lipase activity for herbex was(78.38±0.02)%and(76.07±0.09)%respectively,while that for ethanolic extract(R_(f)=0.96)was(93.66±0.00)%and(95.88±0.13)%.Conclusions:Results of the present study show that Dioscorea steriscus consists of bioactive compounds that can act as lipase andα-amylase inhibitors and therefore can be useful for the development of functional foods against obesity.It can also be used as a source of lead compounds for designing new ant-obesity therapeuticals.展开更多
In response to the limited effectiveness of existing weight loss food products,we sought to apply machine learning-based virtual screening methods to identify potential anti-obesity functional compounds from medicinal...In response to the limited effectiveness of existing weight loss food products,we sought to apply machine learning-based virtual screening methods to identify potential anti-obesity functional compounds from medicinal and edible plants and validate their in vitro activities.Firstly,we construct and evaluate the machine learning(ML)screening models using Multilayer Perceptron(MLP)and Random Forest(RF)algorithms.The receiver operating characteristic(ROC)curve demonstrates the high accuracy of MLP and RF models in screening for obese-related targets PL(pancreatic lipase)and AMPK(Adenosine 5′-monophosphate activated protein kinase).Subsequently,the tested ML models are employed to screen the constructed database,and Gypenoside LXVI(GYP)and alisol-b-23-acetate(ALI)are identified as compounds exhibiting favorable activity against both targets.The hit compounds are tested for their impact on lipase activity and lipid accumulation.The test results show that GYP and ALI have favorable inhibitory effects on pancreatic lipase(PL),with IC50 of 359.7 and 433.8μg/mL.Furthermore,both GYP and ALI significantly reduced cellular lipid accumulation by 72.89%and 79.01%with the concentration increase to 40μg/mL.The molecular docking results indicate that GYP and ALI can interact with several amino acid residues on the two target proteins,thereby affecting the activity of the target proteins.In conclusion,GYP and ALI can prevent and alleviate obesity by inhibiting PL activity and regulating AMPK signaling factors.We innovatively applied virtual screening based on ML to discover functional factors in food for anti-obesity purposes.This novel computational screening technique holds significant potential in the development of dietary supplements to combat obesity.展开更多
Monascus pigments(MPs),the secondary metabolites of Monascus-fermented foods,have been widely studied for their anti-obesity effects.However,the specific targets and potential mechanism of MPs for anti-obesity remain ...Monascus pigments(MPs),the secondary metabolites of Monascus-fermented foods,have been widely studied for their anti-obesity effects.However,the specific targets and potential mechanism of MPs for anti-obesity remain unclear.In this study,we applied network pharmacology and computer simulation to investigate the anti-obesity potential and action mechanism of MPs.Based on public databases,117 potential therapeutic obesity targets of MPs were predicted.Five key targets(STAT3,ESR1,BCL2,PPARG,and MMP9)were obtained by protein-protein interaction(PPI)analysis and microarray data validation.The enrichment results showed that MPs exerted anti-obesity effects through multiple targets and multiple pathways,especially insulin resistance.Molecular docking demonstrated the binding of five key targets to MPs ligands through hydrophobic interactions and hydrogen bonding.Molecular dynamics simulation and binding free energy analysis revealed a higher stability in the binding of ESR1,BCL2,and PPARG targets with MPs ligands.In addition,free energy landscape(FEL)and independent gradient model(IGM)analysis showed that van der Waals forces were also the main driving force in stabilizing the binding of key targets and MPs ligands.This study systematically illustrated the potential anti-obesity mechanism of MPs and established the foundation for the development and application of MPs as anti-obesity functional additives.展开更多
In recent years,obesity has emerged as a significant global public health issue,leading to serious physical health risks and warranting the attention of everyone.Dietary intervention is one of the most important strat...In recent years,obesity has emerged as a significant global public health issue,leading to serious physical health risks and warranting the attention of everyone.Dietary intervention is one of the most important strategies for treating obesity,and fermented Laminaria Japonica(LJ)has shown promising potential as an anti-obesity agent.However,there is currently a lack of comprehensive studies on this topic,necessitating further validation and research to fully understand its efficacy and mechanisms.In the study,we explored the fermentation process,active ingredients,and anti-obesity effects of fermented LJ.Optimal fermentation conditions were determined using response surface methodology:a temperature of 34.54℃,pH of 9.52,and inoculum volume of 9%.HPLCMS/MS analysis identified active compounds,with anti-obesity potential,including monosaccharides,amino acids,and fatty acids.In a high-fat diet induced obese mouse model,treatment with high concentrations of fermented Laminaria Japonica significantly improved obesity symptoms.Additionally,the gut microbiota structure was altered,showing increased abundance of beneficial bacteria(Bacteroidetes,Akkermansia,and Muribaculaceae)and decreased abundance of obesity-associated bacteria(Colidextribacter,Acetatifactor,and Blautia).Functional predictions suggested that the anti-obesity effects may be mediated through pathways related to secondary metabolite synthesis,carbon metabolism,and fatty acid metabolism,thus informing subsequent experiments.In conclusion fermented Laminaria Japonica shows promise as a potential treatment for obesity.展开更多
In this study,an in vitro screening method for lactic acid bacteria(LAB)with anti-obesity and antioxidant effects was developed.We first screened high cholesterol-lowering LAB from the traditional fermented food water...In this study,an in vitro screening method for lactic acid bacteria(LAB)with anti-obesity and antioxidant effects was developed.We first screened high cholesterol-lowering LAB from the traditional fermented food water kefir.Among the screened LAB,there were 12 strains with a cholesterol degradation rate over of 60%.The 12 strains were ranked from highest to lowest cholesterol degradation ability,and the top five strains were selected for subsequent tests to determine their ability to lower triglycerides,inhibitα-amylase andα-glucosidase,and LAB with great anti-obesity and antioxidant effects were screened in vitro by determining their ability to inhibit Enterobacter cloacae and their antioxidant activity.A comprehensive evaluation model was then combined with principal component analysis:Y=0.513Y_(1)+0.229Y_(2)+0.137Y_(3)+0.121Y_(4).Lactobacillus rhamnosus S51,Lactobacillus plantarum S184,and Lactobacillus fermentum S7 had better anti-obesity and antioxidant potential.The cholesterol-lowering ability of S51 was 69.90%,the triglyceride-lowering ability was 53.72%,α-amylase,andα-glucosidase inhibiting ability was 69.97%and 31.49%,respectively,and Enterobacter cloacae inhibiting ability was 3.43 cm.Its IC scavenging ability of DPPH free radical was 90.47%,which was equivalent to 27.98μg/mL Trolox standard solution,the CFE scavenging capacity for hydroxyl radicals was 60.99%,and the total reducing power of CFE was equivalent to 513.37μmol/L L-cysteine hydrochloride.S51,S184,and S7 were eligible for probiotic conditions.The results of the study provide a theoretical basis for the screening of LAB with anti-obesity and antioxidant effects in vitro.展开更多
Germination,elicitation,and fermentation are biotechnological methods that could be applied to improve phytochemical profile and functional quality of edible seeds.This research investigated the combined effects of el...Germination,elicitation,and fermentation are biotechnological methods that could be applied to improve phytochemical profile and functional quality of edible seeds.This research investigated the combined effects of elicitation,germination,and fermentation on the anti-obesity activities of alfalfa seeds.Alfalfa seeds were germinated at 25-27℃ for 3 days while spraying with tap water(control)or 1%of different plant elicitors(sodium chloride,citric acid,or ascorbic acid)solution.Ascorbic acid was the most suitable elicitor to improve phytochemical quality,support growth,and maximize the health-promoting properties of alfalfa sprouts.Treatment with 1%ascorbic acid solution improved lipase(74.57%vs.41.32%),DPPH(76.58%vs.57.21%),and ABTS(71.63%vs.46.5%)inhibitory activities of alfalfa sprouts compared to control.Fermentation of the ascorbic acid-elicited sprouts further enhanced the anti-obesity activities in vitro by improving their inhibitory potential against digestive enzymes,alpha-glucosidase(67.84%vs.81.39%)and pancreatic lipase(74.57%vs.82.07%).The fermented sprout extracts prolonged the lifespan and decreased fat accumulation in the glucose-induced obese Caenorhabditis elegans via mechanisms including suppression of genes involved in fat synthesis and changing the worms’metabolite profiles.Specifically,the extract down-regulated genes involved in fat synthesis(fat-4,fat-6 and fat-7),up-regulated genes for fat lipolysis(hosl-1)and lifespan gene(daf-16),while enhancing amino acids profiles in C.elegans.These findings demonstrate that alfalfa sprouts produced through a combination of ascorbic acid treatment and fermentations could be an effective functional ingredient targeting obesity.展开更多
Crude Cordyceps militaris polysaccharides(CMPs)have been proven to be effective in alleviating harmful effects of a long-term high-fat diet(HFD),but there are fewer related studies on identifying the health-regulating...Crude Cordyceps militaris polysaccharides(CMPs)have been proven to be effective in alleviating harmful effects of a long-term high-fat diet(HFD),but there are fewer related studies on identifying the health-regulating abilities of certain subcomponents of CMPs.This study aimed to figure out the main component of CMPs in anti-obesity and to further explore its regulatory effects in vivo.Results showed among three main subcomponents(CMP40,CMP60 and CMP80)of CMPs,the large molecular weight CMP40(with average molecular weight of more than 100 kDa)has shown superior in vitro hypoglycemic and hypolipidemic capacities.The simultaneous intervention of CMP40 indicated that it could reduce the body weight and white adipose tissue mass/area in the HFD mice.Serum parameters also suggested a decrease in the cholesterol and triglyceride level in the CMP40 group.These results might be due to the down-regulation of lipid synthesis and the up-regulation of lipolysis pathways.Besides,CMP40 could alleviate the inflammatory condition,such as the lower LPS and inflammatory cytokines levels,in the HFD mice in a dose-dependent manner.It could also restore gut health by increasing the SCFAs level,regulating gut microbiota structure,and repairing intestinal tight junction.Results also confirmed safe in CMP40 consumption since there were no side-effects on a high concentration addition of CMP40(8%)to a regular diet.These findings suggested the promising anti-obesity effect of CMP40 and provided a theoretical basis of its further application in food industry.展开更多
基金The financial support received from the Shenzhen Science and Technology Innovation Commission(KCXFZ20201221173207022,WDZC20200821141349001)Shenzhen Bay Laboratory Startup Fund(21310041,S234602003)。
文摘Pu-erh tea,a traditional Chinese beverage,performs an anti-obesity function,but the correlation between its components and efficacy remains unknown.Here,we screened two Pu-erh teas with significant anti-obesity efficacies from 11 teas.In vitro experiments revealed that lipid accumulation in L02 cells and lipid synthesis in 3T3-L1 cells were significantly better inhibited by Tea-B than Tea-A.Further in vivo experiments using model mice revealed that the differences in chemical components generated two pathways in the anti-obesity efficacy and mechanism of Pu-erh teas.Tea-A changes the histomorphology of brown adipose tissue(BAT)and increases the abundance of Coriobacteriaceae_UCG_002 and cyclic AMP in guts through high chemical contents of cyclopentasiloxane,decamethyl,tridecane and 1,2,3-trimethoxybenzene,eventually increasing BAT activation and fat browning gene expression;the high content of hexadecane and 1,2-dimethoxybenzene in Tea-B reduces white adipose tissue(WAT)accumulation and the process of fatty liver,increases the abundance of Odoribacter and sphinganine 1-phosphate,inhibits the expression of lipid synthesis and transport genes.These mechanistic findings on the association of the representative bioactive components in Pu-erh teas with the anti-obesity phenotypes,gut microbes,gut metabolite structure and anti-obesity pathways,which were obtained for the first time,provide foundations for developing functional Pu-erh tea.
文摘Obesity is a major driver of metabolic dysfunction-associated steatotic liver disease(MASLD)and its progressive form,metabolic dysfunction-associated steatohepatitis(MASH).As the global prevalence of obesity continues to rise,the burden of MASLD/MASH is increasing,posing significant challenges to healthcare systems.The use of anti-obesity medications(AOMs)in this population is complex due to altered hepatic metabolism,safety concerns,and potential hepatotoxicity.Recent advances in pharmacologic agents,such as glucagon-like peptide1(GLP-1)receptor agonists(GLP-1 RAs),dual GLP-1/glucose-gastric inhibitory polypeptide(GIP)agonists,and triple GLP-1/GIP/glucagon agonists,have shown promising metabolic effects in the general population.Among these,GLP-1 RAs(e.g.,liraglutide and semaglutide)consistently demonstrate hepatic benefits,including reductions in hepatic steatosis,improvements in liver enzyme profiles,and attenuation of fibrosis progression.Tirzepatide,a dual GLP-1/GIP agonist,has shown superior weight loss effects compared to GLP-1 receptor agonist monotherapy,with emerging but still limited data on hepatic outcomes in MASLD/MASH.Retatrutide,a triple agonist,has produced the most pronounced metabolic effects to date,although its impact on liver histology remains underexplored.Other AOMs,such as bupropion-naltrexone and phentermine-topiramate,require cautious use due to potential hepatotoxicity.Importantly,advanced MASLD may alter drug pharmacokinetics,underscoring the need for individualized therapy and close monitoring.This review provides an updated synthesis of the efficacy and safety of current and emerging AOMs in patients with MASLD/MASH and highlights the urgent need for further research to define optimal pharmacological strategies in this high-risk population.
基金supported by the National Natural Science Foundation of China(No.41806182)the National Key R&D Program of China(No.2020YFD0900900)+1 种基金the Provincial Universities Basic Scientific Research Foundation in Zhejiang Province of China(No.2021ZJ004)the Science and Technology Project of Zhoushan(No.2020C21020).
文摘Swimming crab(Portunus trituberculatus)eggs contain abundant phospholipids and polyunsaturated fatty acids with specific bioactivities.However,more studies on the functions of other nutrients are needed.Here,a novel sialoglycopeptide from P.trituberculatus eggs,which was termed as Pt-SGP,was isolated with enzymolysis.Pt-SGP was a 6480-Da pure sialoglycopeptide.O-glycan units existed between peptides and sugars.Pt-SGP was composed of 10.69%protein,71.43%hexose,and 11.27%Neu5Gc acid.Analyzed with HPLC,GC-MS,and NMR spectrometry,the carbohydrate chain of Pt-SGP was{(4GlcNAc1-2Man1-3)/(4GlcNAc1-2Man1-6)Man1-6}/{4Glc1-4}Glc1-4Glc1-3GlcNAc1.The MS spectrometry analysis result showed that the peptide chain was Val-Ala-Thr*-Val-Ser*-Leu-Pro-Arg.The results on mice showed Pt-SGP could reduce body weight gain by 18.13%,white adipose weight by 28.57%,serum TC by 22.32,serum TG by 9.46%,serum LDL-C by 27.69%,hepatic TC by 73.21%,and hepatic TG by 25.20%.Furthernore,it could increase serum HDL-C by 75.96%,mitigate adipocyte size and hepatic lipid accumulation in high fat diet-fed mice.Pt-SGP significantly down-regulated C/EBPαsignaling and up-regulated AMPKαsignaling in the epididymal fat tissues.The findings indicate that Pt-SGP exhibits notable anti-obesity properties,and can potentially become a promising dietary supplement for anti-obesity.
文摘Although it is not generally a life-threatening disease,obesity is becoming a major health problem worldwide.It can be controlled by means of drugs,and,consequently,these are required to be safe as well as effective.In this paper,we summarize the fate of various drugs that have been introduced for clinical use in the treatment of obesity.Fenfluramine and dexfenfluramine were withdrawn because of heart valve damage.Sibutramine suppresses appetite and increases heart rate and blood pressure.In the Sibutramine Cardiovascular OUTcomes trial,an increase in major adverse cardiovascular events prompted its withdrawal in Europe and the United States.Rimonabant is an endocannabinoid receptor antagonist that reduces body weight and ameliorates some cardiovascular risk factors.However,adverse psychiatric side effects led to its withdrawal as well.Orlistat is approved in Europe and the United States for the treatment of obesity,but its use is limited by gastrointestinal side-effects.Ephedrine and caffeine are natural ingredients in foods and supplements that may help the person to lose weight.In the light of several failed attempts,there is a clear need to develop drugs that are effective and safe in the long term in order to successfully combat the phenomenon of obesity.
文摘Pu-erh tea, a traditional Chinese beverage, has been believed to have many benefits to human health and without side effects. In this study, we systematically analyzed the main active components of Pu-erh tea and investigated its anti-obesity, anti-atherosclerotic and anti-oxidant effects using an obese rat model. Obesity was induced by feeding a high-fat diet and subsequently the experimental obese mice were fed with high-fat diet supplemented with low (2.5%), medium (5%) or high (7.5%) doses of Pu-erh tea powder for 6 weeks respectively. As result, the body weight gain of the rats was decreased by medium and high doses of Pu-erh tea treatments. Furthermore, the levels of serum total cholesterol (TC), triglyceride (TG) and atherosclerosis index (AI) were significantly lowered by Pu-erh tea compared to the control group. Conversely, high density lipoproteincholesterol (HDL-C) level of the rats was significantly elevated by Pu-erh tea treatments. In addition, Pu-erh tea treatments increased the activities of anti-oxidative enzymes such as superoxide dismutase (SOD) and glutathione peroxides (GSH-Px), whereas reduced the level of lipid peroxidation product malondialdehyde (MDA) in obese rats. Collectively, our find-ings revealed that Pu-erh tea exerts comprehensive benefits in anti-obesity, anti-atherosclerotic and anti-oxidant effects, therefore can be used as a promising functional food in obesity management.
文摘Mesotherapy and anti-obesity medications are gradually gaining worldwide popularity for purposes of body contouring and weight loss.Their adverse effects are various,but there is a tendency to disregard them.Ischemic colitis is one of the most common diseases associated with non-obstructive blood vessel disorders.However,there have been no case reports about the adverse effects resulting from mesotherapy only or in combination with anti-obesity medications.We report on an interesting case of ischemic colitis after mesotherapy combined with anti-obesity medications in a 39-year-old female who had no risk factors.
基金supported by the priority academic program development of Jiangsu Higher Education institutionsthe National Natural Science Foundation of China [31801538]
文摘Objective To further explore associated effects of Lactobacillus plantarum dy-1(LFBE) on obesity and lipid metabolism at the gene expression level, the expression of micro RNAs(mi RNAs) was investigated in the liver of high-fat diet(HFD) induced obese rats.Methods Three groups of animal models were established. Changes in mi RNA expression in the liver of each group were analyzed by microarray and RT-q PCR, complemented by bioinformatics. Palmitateinduced hepatocellular carcinoma(Hep G2) cells were used as a model to validate the test.Results LFBE treatment groups and HFD groups were observed to be distinctly different with respect to rates of increase in body weight and body fat percentage and triglyceride(TG) and total cholesterol(TC) levels in serum and liver. In addition, the LFBE group showed upregulation of ten mi RNAs and downregulation of five mi RNAs in the liver. Downregulation of mi R-34 a and mi R-212 was observed in the livers of the LFBE group. Gene ontology and kyoto encyelopedia of geues and genomes(KEGG)pathway analysis showed that possible target genes of the deregulated mi RNAs were significantly enriched in the adrenergic and HIF-1 signaling pathways.Conclusion These results demonstrate that LFBE might regulate the expression of mi RNAs in order to inhibit obesity and fatty liver.
基金Supported by the Ministry of Science and Technology of China(2008AA12A218 2009BAK59B01)
文摘Semicarbazide-sensitive the metabolism of glucose and fat, amine oxidase （SSAO） has been considered to be associated with and elevated SSAO activity was observed in obese patients. In the present study, an in vitro SSAO activity-based method was developed to screen inhibitors from 15 an- ti-obesity drugs. Among the fifteen anti-obesity drugs, four drugs including caffeine, fenfluramine, bumetanide and amfebutamone inhibited SSAO activity, and caffeine was the most effective one. When the concentration of caffeine was 1.4 mmol/L, the inhibition ratio was 31.9% and 18.8% in rabbit serum and rat adipose tissue, respectively. Inhibition of SSAO activity by caffeine was also confirmed in the in vivo study, showing the inhibition ratio of 15.6% on serum SSAO. Caffeine pro- vides a natural source of inhibition of SSAO activity and may be a promising inhibitor for the study of SSAO.
文摘As a dark tea,Pu-erh tea(PET)is produced from sun-dried leaves of Camellia sinensis var.assamica mainly in Yunnan Province of China.Many microorganisms are involved in the fermentation of PET.Among them,Aspergillus niger is most important.It is believed that the longer the preservation period,the better is the quality and taste of PET,which is commercially available as loose,compressed or instant tea leaves.Chemical components of PET include flavones,flavanols,flavonols,phenolic acids,alkaloids and methylxanthines.In this overview,the lipid-lowering and anti-obesity effects of PET were discussed based on animal models and human trials,and our study provided some insights into possible mechanisms of bioactive compounds,such as theabrownin,catechins,lovastatin and gallic acid.Other bioactivities of PET and some information on Fuzhuan brick tea were also included.Sources of information cited were from Google Scholar,Pub Med,Pub Med Central,Science Direct,J-Stage,Pub Chem,Directory of Open Access Journals(DOAJ),and China National Knowledge Infrastructure(CNKI).
基金Fundamental Research Funds for the Central Universities of China(2662019PY066,2662016PY132)。
文摘This study investigated hypolipidemic,weight-reducing,and hepatoprotective effects of Antrodia cinnamomea mycelial extract obtained from solid-state culture(ACME)in an HFr D-induced obese/hyperlipidemic mouse model.Following 4-week ACME treatment,body weight,epididymal fat index,and some serum biochemical indices were measured.Expression levels of some related genes involved in cholesterol and lipid metabolism were analyzed by q RT-PCR.Moreover,histological studies of hepatic tissues were also conducted.After ACME treatment,body weight and epididymal fat index were significantly lower than that in model control group.ACME and simvastatin significantly reduced serum total cholesterol(T-CHO)and low-density lipoprotein cholesterol(LDL-C)levels,and increased high-density lipoprotein cholesterol(HDL-C)level.Subsequent experiments showed that:(i)ACME regulated transcriptional expression of 3-hydroxy-3-methylglutaryl-Co A reductase(HMGR),low-density lipoprotein receptor(LDLR),adipose triglyceride lipase(ATGL),and fatty acid synthase(FAS),with consequent reduction of blood lipid levels and body weight;(ii)ACME enhanced total antioxidant capacity(T-AOC)and superoxide dismutase(SOD)activity in hepatic tissues;(iii)ACME reduced malondialdehyde(MDA)level and ameliorated lipid oxidative damage in liver.Our findings indicate that ACME is a strong candidate for development as a novel anti-hyperlipidemia and anti-obesity health product.
基金The“Basic Science Research Program”extended its support via the National Research Foundation of Korea (NRF),which is sponsored through the Ministry of Education (2018R1C1B6004780)supported by Main Research Program (E0211200-03)of the Korea Food Research Institute (KFRI)funded by the Ministry of Science and ICT。
文摘The prevalence of obesity has increased and is a health concern worldwide.Due to the concerns regarding synthetic anti-obesity treatments,nowadays natural products become a trend.Previous studies proved that there is a potential to use marine algae as anti-obesity agents.Therefore,in this study,the lipid inhibitory effect of crude polysaccharide of amyloglucosidase-assisted hydrolysate from Sargassum thunbergii(STAC)and its fucoidan fractions(STAFs)on 3T3-L1 cells and high-fat diet(HFD)-induced obese mice were investigated.According to the results,the STAF3,showed the highest xylose content and exhibited significant inhibitory effects on lipid accumulation by downregulating adipogenic and lipogenic proteins in 3T3-L1 cells.Furthermore,oral supplementation with STAC significantly declined gain in body weight and fat weight,and serum lipid contents in an HFD-induced obesity mouse model.Structural and chemical characterizations demonstrated that puritied STAF3 has consistent surface morphology and small particle size,with similar structural characteristics as commercial fucoidan.Together,these results indicate that STAC and purified STAF3 from Sargassum thunbergia is a potent source to develop as ananti-obesity agents or functional food products to counter obesity.
基金supported by National Natural Science Foundation of China(Project No.32160547)Central Government Guide Local Special Fund Project(Project No.20212ZDD02008)+2 种基金Vegetable Industry Technology System Post Expert Project of Jiangxi Province(Project No.JXARS-06)State Key Laboratory of Food Science and Technology Nanchang University(Project No.SKLF-ZZA-201910)State Key Laboratory of Food Science and Technology Nanchang University(Project No.SKLF-ZZB-202132).
文摘The worldwide obesity epidemic has imposed human being a huge burden and attention had been drawn to manipulating the lactic acid bacteria (LAB) to improve obesity symptoms.In this study,a more comprehensive and reliable in vitro method was established to screen LAB with greater potential against obesity.Firstly,twenty-eight human feces-derived LAB strains were selected by assessing bile salt-acid tolerance and cholesterol lowering capacity.Subsequently,the triglyceride lowering capacity,bile salt hydrolase (BSH) activity,α-glucosidase activity inhibition capacity and Enterobacter cloacae (E.cloacae) growth inhibition capacity were tested for further evaluating the anti-obesity properties of LAB strains.Principal component analysis (PCA) of these results indicated that Limosilactobacillus reuteri (L.reuteri) NCUH003,Lactiplantibacillus plantarum (L.plantarum) NCUH046 and Limosilactobacillus fermentum (L.fermentum) NCUH068 exhibited favorable anti-obesity properties.Besides,the LAB displayed good gastrointestinal adaptability and antioxidant activity but no hemolytic activity,and they were sensitive to various antibiotics.These results indicated that L.reuteri NCUH003,L.plantarum NCUH046 and L.fermentum NCUH068 are potential for application in obesity treatment.
基金supported by[National Natural Science Foundation of China](Grant number 32001702).
文摘This study aimed to elucidate the inhibition mechanism of apigenin against porcine pancreatic lipase(PPL),and,moreover,to comprehensively reveal the molecular basis of its anti-obesity via network pharmacology approach.The results showed that apigenin inhibited PPL with an IC50 value of 0.377±0.041 mM.Spectroscopic techniques combined molecular docking suggested that apigenin could bind into the PPL active pocket,affecting its normal spatial conformation.Moreover,molecular dynamic(MD)simulations revealed that the open conformation of PPL tended to transit to the closed in the presence of apigenin,which might one important reason for the inhibition of PPL catalytic ability.Network pharmacology analysis revealed that a total of 49 proteins could be identified as potential targets for the anti-obesity effects of apigenin.According to the protein-protein interaction(PPI)network analysis,six hub targets were extracted including IGF1,ESR1,MMP9,PPARA,MAPK14 and NR3C1.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment indicated that the 49 potential targets could be mapped to 30 pathways(p<0.05).Among them,PI3K-Akt signaling pathway and insulin resistance can be considered as two major pathways regulated by apigenin.Further docking studies indicated that apigenin can bind into the binding pocket of the six hub target proteins identified according to the PPI network.The results indicated that in addition to inhibiting PPL,apigenin could exhibit anti-obesity benefit through the molecular mechanisms uncovered by network pharmacology.This study proposes a new strategy to reveal the mechanisms of dietary polyphenols at the level of network pharmacology.
基金Supported by Bindura University of Science Education(Grant No.2013/6).
文摘Objective:To investigate antioxidant and anti-obesity activity of flavonoids extracted by solvent cold percolation and preparative thin liquid chromatography from Dioscorea steriscus tubers.Methods:1-diphenyl-2-picrylhydrazyl(DPPH)antiradical activity was employed to investigate antioxidant activity while chromogenic method was used to determine alpha amylase inhibition activity and spectrophotometric methods using triolein as a substrate was used to investigate lipase activity.Results:Thin liquid chromatography profiling revealed eight different flavonoid types.Ethyl acetate extract yielded two types,R_(f) values 0.38 and 0.40;chloroform extract also yielded two types R_(f) values 0.06 and 0.51,while ethanol extract yielded four types with R_(f) values 0.16,0.33,0.65 and 0.96.All the extracted flavonoids exhibited antioxidant activity with ethanol extracts exhibiting the greatest antiradical activity.The order of enzyme inhibition capacity was ethyl acetate<chloroform<ethanol.Ethanol extract exhibited significantly greater anti-obesity activity as compared to herbex,a commercially anti-obesity medication sold in drug stores.Anti-α amylase activity and anti-lipase activity for herbex was(78.38±0.02)%and(76.07±0.09)%respectively,while that for ethanolic extract(R_(f)=0.96)was(93.66±0.00)%and(95.88±0.13)%.Conclusions:Results of the present study show that Dioscorea steriscus consists of bioactive compounds that can act as lipase andα-amylase inhibitors and therefore can be useful for the development of functional foods against obesity.It can also be used as a source of lead compounds for designing new ant-obesity therapeuticals.
基金supported by National Natural Science Foundation of China(NSFC)(Contract No.32172234).
文摘In response to the limited effectiveness of existing weight loss food products,we sought to apply machine learning-based virtual screening methods to identify potential anti-obesity functional compounds from medicinal and edible plants and validate their in vitro activities.Firstly,we construct and evaluate the machine learning(ML)screening models using Multilayer Perceptron(MLP)and Random Forest(RF)algorithms.The receiver operating characteristic(ROC)curve demonstrates the high accuracy of MLP and RF models in screening for obese-related targets PL(pancreatic lipase)and AMPK(Adenosine 5′-monophosphate activated protein kinase).Subsequently,the tested ML models are employed to screen the constructed database,and Gypenoside LXVI(GYP)and alisol-b-23-acetate(ALI)are identified as compounds exhibiting favorable activity against both targets.The hit compounds are tested for their impact on lipase activity and lipid accumulation.The test results show that GYP and ALI have favorable inhibitory effects on pancreatic lipase(PL),with IC50 of 359.7 and 433.8μg/mL.Furthermore,both GYP and ALI significantly reduced cellular lipid accumulation by 72.89%and 79.01%with the concentration increase to 40μg/mL.The molecular docking results indicate that GYP and ALI can interact with several amino acid residues on the two target proteins,thereby affecting the activity of the target proteins.In conclusion,GYP and ALI can prevent and alleviate obesity by inhibiting PL activity and regulating AMPK signaling factors.We innovatively applied virtual screening based on ML to discover functional factors in food for anti-obesity purposes.This novel computational screening technique holds significant potential in the development of dietary supplements to combat obesity.
基金supported by the National Natural Science Foundation of China(No.32372298)the Xinjiang‘Two Zones’Science and Technology Development Plan Project(No.2022LQ02003)+3 种基金the Beijing Municipal Natural Science Foundation-Beijing Municipal Education Commission Science and Technology Plan Key Joint Project(No.KZ202010011016)the Construction of High-precision Disciplines in Beijing-Food Science&Engineering(No.19008021085)the fund of Cultivation Project of Double First-Class Disciplines of Food Science and Engineering(No.BTBUYXTD202208)the Construction of China Food Flavor and Nutrition Health Innovation Center(No.19008023032).
文摘Monascus pigments(MPs),the secondary metabolites of Monascus-fermented foods,have been widely studied for their anti-obesity effects.However,the specific targets and potential mechanism of MPs for anti-obesity remain unclear.In this study,we applied network pharmacology and computer simulation to investigate the anti-obesity potential and action mechanism of MPs.Based on public databases,117 potential therapeutic obesity targets of MPs were predicted.Five key targets(STAT3,ESR1,BCL2,PPARG,and MMP9)were obtained by protein-protein interaction(PPI)analysis and microarray data validation.The enrichment results showed that MPs exerted anti-obesity effects through multiple targets and multiple pathways,especially insulin resistance.Molecular docking demonstrated the binding of five key targets to MPs ligands through hydrophobic interactions and hydrogen bonding.Molecular dynamics simulation and binding free energy analysis revealed a higher stability in the binding of ESR1,BCL2,and PPARG targets with MPs ligands.In addition,free energy landscape(FEL)and independent gradient model(IGM)analysis showed that van der Waals forces were also the main driving force in stabilizing the binding of key targets and MPs ligands.This study systematically illustrated the potential anti-obesity mechanism of MPs and established the foundation for the development and application of MPs as anti-obesity functional additives.
基金supported by Zhengying Foods Co.,Ltd.(Project Code:92220259).
文摘In recent years,obesity has emerged as a significant global public health issue,leading to serious physical health risks and warranting the attention of everyone.Dietary intervention is one of the most important strategies for treating obesity,and fermented Laminaria Japonica(LJ)has shown promising potential as an anti-obesity agent.However,there is currently a lack of comprehensive studies on this topic,necessitating further validation and research to fully understand its efficacy and mechanisms.In the study,we explored the fermentation process,active ingredients,and anti-obesity effects of fermented LJ.Optimal fermentation conditions were determined using response surface methodology:a temperature of 34.54℃,pH of 9.52,and inoculum volume of 9%.HPLCMS/MS analysis identified active compounds,with anti-obesity potential,including monosaccharides,amino acids,and fatty acids.In a high-fat diet induced obese mouse model,treatment with high concentrations of fermented Laminaria Japonica significantly improved obesity symptoms.Additionally,the gut microbiota structure was altered,showing increased abundance of beneficial bacteria(Bacteroidetes,Akkermansia,and Muribaculaceae)and decreased abundance of obesity-associated bacteria(Colidextribacter,Acetatifactor,and Blautia).Functional predictions suggested that the anti-obesity effects may be mediated through pathways related to secondary metabolite synthesis,carbon metabolism,and fatty acid metabolism,thus informing subsequent experiments.In conclusion fermented Laminaria Japonica shows promise as a potential treatment for obesity.
基金the national first-class discipline program of Light Industry Technology and Engineering(LITE2018-11)the Key Laboratory of Industrial Biotechnology(Jiangnan University,China)+1 种基金Ministry of Education,and the Key Laboratory of Carbohydrate Chemistry&Biotechnology(Jiangnan University,China)Ministry of Education,for financial support.
文摘In this study,an in vitro screening method for lactic acid bacteria(LAB)with anti-obesity and antioxidant effects was developed.We first screened high cholesterol-lowering LAB from the traditional fermented food water kefir.Among the screened LAB,there were 12 strains with a cholesterol degradation rate over of 60%.The 12 strains were ranked from highest to lowest cholesterol degradation ability,and the top five strains were selected for subsequent tests to determine their ability to lower triglycerides,inhibitα-amylase andα-glucosidase,and LAB with great anti-obesity and antioxidant effects were screened in vitro by determining their ability to inhibit Enterobacter cloacae and their antioxidant activity.A comprehensive evaluation model was then combined with principal component analysis:Y=0.513Y_(1)+0.229Y_(2)+0.137Y_(3)+0.121Y_(4).Lactobacillus rhamnosus S51,Lactobacillus plantarum S184,and Lactobacillus fermentum S7 had better anti-obesity and antioxidant potential.The cholesterol-lowering ability of S51 was 69.90%,the triglyceride-lowering ability was 53.72%,α-amylase,andα-glucosidase inhibiting ability was 69.97%and 31.49%,respectively,and Enterobacter cloacae inhibiting ability was 3.43 cm.Its IC scavenging ability of DPPH free radical was 90.47%,which was equivalent to 27.98μg/mL Trolox standard solution,the CFE scavenging capacity for hydroxyl radicals was 60.99%,and the total reducing power of CFE was equivalent to 513.37μmol/L L-cysteine hydrochloride.S51,S184,and S7 were eligible for probiotic conditions.The results of the study provide a theoretical basis for the screening of LAB with anti-obesity and antioxidant effects in vitro.
基金funded by the Cooperative Research Program for Agriculture Science and Technology Development(Project No.PJ0145652021)Rural Development Administration,Korea.
文摘Germination,elicitation,and fermentation are biotechnological methods that could be applied to improve phytochemical profile and functional quality of edible seeds.This research investigated the combined effects of elicitation,germination,and fermentation on the anti-obesity activities of alfalfa seeds.Alfalfa seeds were germinated at 25-27℃ for 3 days while spraying with tap water(control)or 1%of different plant elicitors(sodium chloride,citric acid,or ascorbic acid)solution.Ascorbic acid was the most suitable elicitor to improve phytochemical quality,support growth,and maximize the health-promoting properties of alfalfa sprouts.Treatment with 1%ascorbic acid solution improved lipase(74.57%vs.41.32%),DPPH(76.58%vs.57.21%),and ABTS(71.63%vs.46.5%)inhibitory activities of alfalfa sprouts compared to control.Fermentation of the ascorbic acid-elicited sprouts further enhanced the anti-obesity activities in vitro by improving their inhibitory potential against digestive enzymes,alpha-glucosidase(67.84%vs.81.39%)and pancreatic lipase(74.57%vs.82.07%).The fermented sprout extracts prolonged the lifespan and decreased fat accumulation in the glucose-induced obese Caenorhabditis elegans via mechanisms including suppression of genes involved in fat synthesis and changing the worms’metabolite profiles.Specifically,the extract down-regulated genes involved in fat synthesis(fat-4,fat-6 and fat-7),up-regulated genes for fat lipolysis(hosl-1)and lifespan gene(daf-16),while enhancing amino acids profiles in C.elegans.These findings demonstrate that alfalfa sprouts produced through a combination of ascorbic acid treatment and fermentations could be an effective functional ingredient targeting obesity.
基金support for this work provided by seed industry revitalization project of rural revitalization strategy of Guangdong Province(2022-WPY-00-006)the project of Natural Science Foundation of Guangdong Province(Grant No.2022A1515010057)Guangdong Province Agriculture Research Project&Agricultural Technique Promotion Project(2019KJ103,2024KJ103).
文摘Crude Cordyceps militaris polysaccharides(CMPs)have been proven to be effective in alleviating harmful effects of a long-term high-fat diet(HFD),but there are fewer related studies on identifying the health-regulating abilities of certain subcomponents of CMPs.This study aimed to figure out the main component of CMPs in anti-obesity and to further explore its regulatory effects in vivo.Results showed among three main subcomponents(CMP40,CMP60 and CMP80)of CMPs,the large molecular weight CMP40(with average molecular weight of more than 100 kDa)has shown superior in vitro hypoglycemic and hypolipidemic capacities.The simultaneous intervention of CMP40 indicated that it could reduce the body weight and white adipose tissue mass/area in the HFD mice.Serum parameters also suggested a decrease in the cholesterol and triglyceride level in the CMP40 group.These results might be due to the down-regulation of lipid synthesis and the up-regulation of lipolysis pathways.Besides,CMP40 could alleviate the inflammatory condition,such as the lower LPS and inflammatory cytokines levels,in the HFD mice in a dose-dependent manner.It could also restore gut health by increasing the SCFAs level,regulating gut microbiota structure,and repairing intestinal tight junction.Results also confirmed safe in CMP40 consumption since there were no side-effects on a high concentration addition of CMP40(8%)to a regular diet.These findings suggested the promising anti-obesity effect of CMP40 and provided a theoretical basis of its further application in food industry.
