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藜麦ANT基因家族的鉴定及其在愈伤组织中的表达分析 被引量:1
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作者 高爱红 张侠 +5 位作者 曹萌 安珂欣 尹海波 郭善利 张平 赵波 《山东农业科学》 北大核心 2025年第4期22-31,共10页
ANT/euANT(AINTEGUMENTA)是一类仅存在于植物中的APETALA2(AP2)型转录因子,隶属于AP2/EREBP家族。本研究利用藜麦基因组数据库,对藜麦ANT基因家族进行鉴定和生物信息学分析,并通过qRT-PCR分析藜麦ANT基因家族成员在不同愈伤组织与芽中... ANT/euANT(AINTEGUMENTA)是一类仅存在于植物中的APETALA2(AP2)型转录因子,隶属于AP2/EREBP家族。本研究利用藜麦基因组数据库,对藜麦ANT基因家族进行鉴定和生物信息学分析,并通过qRT-PCR分析藜麦ANT基因家族成员在不同愈伤组织与芽中的表达。结果表明,在藜麦中共鉴定出13个含有两个AP2保守结构域的ANT基因家族成员,不均匀地分布在藜麦9条染色体上,根据在染色体上的位置将其分别命名为CqANT1—CqANT13。它们编码蛋白的氨基酸长度为244~710 aa,分子量为27.20~77.22 kDa,等电点为5.57~9.21,为不稳定的亲水蛋白,均定位于细胞核。基因共线性分析发现12个CqANT基因组成6对片段复制,未鉴定到串联复制事件。启动子顺式作用元件分析发现,藜麦ANT家族成员的启动子区含有多种激素和环境响应元件,暗示其参与调控藜麦的非生物胁迫及激素信号级联体调控应答等过程。其与双子叶植物拟南芥的ANT同源性较高;表达模式分析结果表明,藜麦ANT基因的表达具有组织特异性,并对非生物胁迫具有一定响应;藜麦ANT基因在发生芽和不同愈伤组织中均有一定的表达,说明其在芽与愈伤组织诱导和维持中发挥重要作用。本研究结果可为进一步揭示藜麦ANT基因的功能和调控机制提供依据。 展开更多
关键词 藜麦 ant基因家族 生物信息学分析 愈伤组织表达
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转译、重组与维系:ANT视角下的纸质相册与家庭关系
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作者 刘艺璇 胡翼青 《山东科技大学学报(社会科学版)》 2025年第1期1-10,共10页
以行动者网络理论为分析视角,通过对20个家庭做深度访谈,探讨数字时代纸质家庭相册的存在意义。研究发现,纸质相册作为行动者实现了对家庭关系的转译:通过家庭成员的共同许诺增设对于“家”的责任关系,通过对记忆的具象显影强化不同成... 以行动者网络理论为分析视角,通过对20个家庭做深度访谈,探讨数字时代纸质家庭相册的存在意义。研究发现,纸质相册作为行动者实现了对家庭关系的转译:通过家庭成员的共同许诺增设对于“家”的责任关系,通过对记忆的具象显影强化不同成员间的情感联结,通过描摹理想家庭图景为成员暗示行为规范;在行动过程中,纸质相册征召众多异质行动者并成为“必经之点”,而后将行动关系铭刻于物质形态,最终构成了纸质相册的行动性来源,使得家庭关系的重组与维系成为可能。 展开更多
关键词 纸质家庭相册 ant 行动者 转译 家庭关系
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Extension of covariant derivative(Ⅰ): From component form to objective form 被引量:4
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作者 Ya-Jun Yin 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2015年第1期79-87,共9页
This paper extends the covariant derivative un der curved coordinate systems in 3D Euclid space. Based on the axiom of the covariant form invariability, the classical covariant derivative that can only act on componen... This paper extends the covariant derivative un der curved coordinate systems in 3D Euclid space. Based on the axiom of the covariant form invariability, the classical covariant derivative that can only act on components is ex tended to the generalized covariant derivative that can act on any geometric quantity including base vectors, vectors and tensors. Under the axiom, the algebra structure of the gen eralized covariant derivative is proved to be covariant dif ferential ring. Based on the powerful operation capabilities and simple analytical properties of the generalized covariant derivative, the tensor analysis in curved coordinate systems is simplified to a large extent. 展开更多
关键词 Tensor analysis Classical covariant derivatives Generalized covariant derivatives The axiom of the covari-ant form invariability Covariant differential ring
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行动者-网络是成功的理论吗?——ANT在国内新闻传播学的应用图景、理论限度与突破路径
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作者 张杰 聂茜 《新闻记者》 北大核心 2025年第8期25-40,共16页
本文探讨了行动者-网络理论(ANT)在新闻传播研究中的应用图景与研究进路,发现因其“非人行动者”的概念及行动偏向为观察数字媒介技术对新闻传播的影响提供了独特视角,故而ANT被广泛应用于新闻创新及媒介研究中。但由于无法进行相对静... 本文探讨了行动者-网络理论(ANT)在新闻传播研究中的应用图景与研究进路,发现因其“非人行动者”的概念及行动偏向为观察数字媒介技术对新闻传播的影响提供了独特视角,故而ANT被广泛应用于新闻创新及媒介研究中。但由于无法进行相对静止面向的结构性和长时段的历史性分析,在开展具体经验研究时,ANT往往无法被独立应用而需进行概念拼装。ANT的理论限度可能导致新闻传播研究的表面化、同质化和功利化。在后续应用中,重视和恢复ANT的动态“追随”视角,在此基础上建构稳定态的组合型经验理论,才是ANT得以延续其理论生命力的关键所在。 展开更多
关键词 行动者-网络理论 ant 非人行动者 转译 型构
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Genomic architecture of ant social evolution
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作者 Weiwei Liu Guo Ding +1 位作者 Zijun Xiong Guojie Zhang 《Zoological Research》 2025年第4期789-791,共3页
Ants rank among the most ecologically dominant and evolutionarily remarkable insects on the planet,capturing the imagination of both curious children and thoughtful scholars alike.Aristotle,impressed by their division... Ants rank among the most ecologically dominant and evolutionarily remarkable insects on the planet,capturing the imagination of both curious children and thoughtful scholars alike.Aristotle,impressed by their division of labor and cooperative behavior,described them as“political animals”.In Aesop’s Fables,they are celebrated for their foresight and diligence in preparing for hardship.Traditional Chinese narratives similarly portray ants as modest creatures that,through collective effort,achieve extraordinary power and influence. 展开更多
关键词 chinese narratives collectiveeffort ants cooperativebehavior divisionoflabor evolutionaryremarkability socialevolution ecologicaldominance
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An adhesive drone trap to study the flight altitude preferences of winged ants
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作者 Daniele Giannetti Enrico Schifani Donato A.Grasso 《Current Zoology》 2025年第5期674-677,共4页
The ability of queens and males of most ant species to disperse by flight has fundamentally contributed to the group’s evolutionary and ecological success and is a determining factor to take into account for biogeogr... The ability of queens and males of most ant species to disperse by flight has fundamentally contributed to the group’s evolutionary and ecological success and is a determining factor to take into account for biogeographic studies(Wagner and Liebherr 1992;Peeters and Ito 2001;Helms 2018). 展开更多
关键词 FLIGHT ALTITUDE winged ants PREFERENCES biogeographic studies wagner ADHESIVE TRAP
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Enhancing Hierarchical Task Network Planning through Ant Colony Optimization in Refinement Process
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作者 Mohamed Elkawkagy Ibrahim A.Elgendy +2 位作者 Ammar Muthanna Reem Ibrahim Alkanhel Heba Elbeh 《Computers, Materials & Continua》 2025年第7期393-415,共23页
Hierarchical Task Network(HTN)planning is a powerful technique in artificial intelligence for handling complex problems by decomposing them into hierarchical task structures.However,achieving optimal solutions in HTN ... Hierarchical Task Network(HTN)planning is a powerful technique in artificial intelligence for handling complex problems by decomposing them into hierarchical task structures.However,achieving optimal solutions in HTN planning remains a challenge,especially in scenarios where traditional search algorithms struggle to navigate the vast solution space efficiently.This research proposes a novel technique to enhance HTN planning by integrating the Ant Colony Optimization(ACO)algorithm into the refinement process.The Ant System algorithm,inspired by the foraging behavior of ants,is well-suited for addressing optimization problems by efficiently exploring solution spaces.By incorporating ACO into the refinement phase of HTN planning,the authors aim to leverage its adaptive nature and decentralized decision-making to improve plan generation.This paper involves the development of a hybrid strategy called ACO-HTN,which combines HTN planning with ACO-based plan selection.This technique enables the system to adaptively refine plans by guiding the search towards optimal solutions.To evaluate the effectiveness of the proposed technique,this paper conducts empirical experiments on various domains and benchmark datasets.Our results demonstrate that the ACO-HTN strategy enhances the efficiency and effectiveness of HTN planning,outperforming traditional methods in terms of solution quality and computational performance. 展开更多
关键词 Hierarchical planning ant system optimization automated planning PANDA planner plan selection strategy
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Bayesian-based ant colony optimization algorithm for edge detection
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作者 YU Yongbin ZHONG Yuanjingyang +6 位作者 FENG Xiao WANG Xiangxiang FAVOUR Ekong ZHOU Chen CHENG Man WANG Hao WANG Jingya 《Journal of Systems Engineering and Electronics》 2025年第4期892-902,共11页
Ant colony optimization(ACO)is a random search algorithm based on probability calculation.However,the uninformed search strategy has a slow convergence speed.The Bayesian algorithm uses the historical information of t... Ant colony optimization(ACO)is a random search algorithm based on probability calculation.However,the uninformed search strategy has a slow convergence speed.The Bayesian algorithm uses the historical information of the searched point to determine the next search point during the search process,reducing the uncertainty in the random search process.Due to the ability of the Bayesian algorithm to reduce uncertainty,a Bayesian ACO algorithm is proposed in this paper to increase the convergence speed of the conventional ACO algorithm for image edge detection.In addition,this paper has the following two innovations on the basis of the classical algorithm,one of which is to add random perturbations after completing the pheromone update.The second is the use of adaptive pheromone heuristics.Experimental results illustrate that the proposed Bayesian ACO algorithm has faster convergence and higher precision and recall than the traditional ant colony algorithm,due to the improvement of the pheromone utilization rate.Moreover,Bayesian ACO algorithm outperforms the other comparative methods in edge detection task. 展开更多
关键词 ant colony optimization(ACO) Bayesian algorithm edge detection transfer function.
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Optimization of broadband omnidirectional antireflection coatings for solar cells 被引量:4
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作者 Xia Guo Qiaoli Liu +5 位作者 Huijun Tian Ben Li Hongyi Zhou Chong Li Anqi Hu Xiaoying He 《Journal of Semiconductors》 EI CAS CSCD 2019年第3期33-38,共6页
Broadband and omnidirectional antireflection coating is generally an effective way to improve solar cell efficiency, because the destructive interference between the reflected and incident light can maximize the light... Broadband and omnidirectional antireflection coating is generally an effective way to improve solar cell efficiency, because the destructive interference between the reflected and incident light can maximize the light transmission into the absorption layer. In this paper, we report the incident quantum efficiency ηin, not incident energy or power, as the evaluation function by the ant colony algorithm optimization method, which is a swarm-based optimization method. Also, SPCTRL2 is proposed to be incorporated for accurate optimization because the solar irradiance on a receiver plane is dependent on position, season, and time. Cities of Quito, Beijing and Moscow are selected for two-and three-layer antireflective coating optimization over λ = [300,1100] nm and θ = [0°, 90°]. The ηin increases by 0.26%, 1.37% and 4.24% for the above 3 cities, respectively, compared with that calculated by other rigorous optimization algorithms methods, which is further verified by the effect of position and time dependent solar spectrum on the antireflective coating design. 展开更多
关键词 antIREFLECTION coating ant COLONY algorithm INCIDENT quantum efficiency SPCTRL2
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生成式人工智能虚假信息的产生机制及协同治理研究——基于行动者网络理论(ANT) 被引量:1
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作者 万宏静 崔琦 《合肥工业大学学报(社会科学版)》 2025年第4期75-83,共9页
生成式人工智能技术的迅速发展加剧了虚假信息的生成与传播,对信息秩序产生了巨大冲击,正逐渐成为严重的社会问题。基于行动者网络理论,从问题呈现、利益赋予、征召与动员四个关键环节,梳理了技术开发者、社交平台、普通用户等多元行动... 生成式人工智能技术的迅速发展加剧了虚假信息的生成与传播,对信息秩序产生了巨大冲击,正逐渐成为严重的社会问题。基于行动者网络理论,从问题呈现、利益赋予、征召与动员四个关键环节,梳理了技术开发者、社交平台、普通用户等多元行动者在虚假信息网络中的角色及其互动关系,揭示了“转译”过程中生成式人工智能虚假信息的内容生成逻辑和驱动因素。研究发现,问题呈现阶段构成了虚假信息生成的网络化起点;利益赋予阶段驱动了虚假信息的具体内容生成;征召阶段扩展并动态调整了虚假信息网络;动员阶段则保障了虚假信息生成网络的长期稳定性。基于上述分析,文章提出了四个方向的协同治理路径:机器目标与人类需求的价值协同;多元行动者的奖惩协同;技术与数据的创新协同;公众参与的监督协同。研究旨在深化对于生成式人工智能虚假信息内容生成过程的复杂性与动态性的理解,以期为有效预防、检测与治理生成式人工智能虚假信息提供策略性参考。 展开更多
关键词 生成式人工智能 虚假信息 行动者网络理论
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添加Na^+对ANT系统介电性能的影响 被引量:2
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作者 李玲霞 郭炜 +3 位作者 吴霞宛 王洪儒 张志萍 余昊明 《无机材料学报》 SCIE EI CAS CSCD 北大核心 2004年第4期823-826,共4页
Ag2O-Nb2O5-Ta2O5陶瓷系统(简称ANT)是一种新型的高频高介陶瓷材料,可以在1150℃以下进行中温烧结。在本系统中,添加Na+离子置换ANT系统中的A位A+离子形成ANNT系统,通过XRD,SEM以及能谱分析发现,少量Na+添加,导致晶格参数下降,即八面体... Ag2O-Nb2O5-Ta2O5陶瓷系统(简称ANT)是一种新型的高频高介陶瓷材料,可以在1150℃以下进行中温烧结。在本系统中,添加Na+离子置换ANT系统中的A位A+离子形成ANNT系统,通过XRD,SEM以及能谱分析发现,少量Na+添加,导致晶格参数下降,即八面体空隙的体积将减小,从而抑制了Nb5+的松弛极化,有效地降低了ANT系统的损耗因子,使系统的介电性能达到:ε>500,tg δ≤5×10-4,容量温度系数αc=+12ppm/℃,ρv>1012Ωcm。 展开更多
关键词 高频高介 ant 松弛极化 介电损耗
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Improved ant colony optimization for multi-depot heterogeneous vehicle routing problem with soft time windows 被引量:10
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作者 汤雅连 蔡延光 杨期江 《Journal of Southeast University(English Edition)》 EI CAS 2015年第1期94-99,共6页
Considering that the vehicle routing problem (VRP) with many extended features is widely used in actual life, such as multi-depot, heterogeneous types of vehicles, customer service priority and time windows etc., a ... Considering that the vehicle routing problem (VRP) with many extended features is widely used in actual life, such as multi-depot, heterogeneous types of vehicles, customer service priority and time windows etc., a mathematical model for multi-depot heterogeneous vehicle routing problem with soft time windows (MDHVRPSTW) is established. An improved ant colony optimization (IACO) is proposed for solving this model. First, MDHVRPSTW is transferred into different groups according to the nearest principle, and then the initial route is constructed by the scanning algorithm (SA). Secondly, genetic operators are introduced, and crossover probability and mutation probability are adaptively adjusted in order to improve the global search ability of the algorithm. Moreover, the smooth mechanism is used to improve the performance of the ant colony optimization (ACO). Finally, the 3-opt strategy is used to improve the local search ability. The proposed IACO was tested on three new instances that were generated randomly. The experimental results show that IACO is superior to the other three existing algorithms in terms of convergence speed and solution quality. Thus, the proposed method is effective and feasible, and the proposed model is meaningful. 展开更多
关键词 vehicle routing problem soft time window improved ant colony optimization customer service priority genetic algorithm
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用酶联免疫-化学发光法测定ANT的实验研究 被引量:3
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作者 张丽民 贺广彩 +2 位作者 陈杞 董长安 龚贻芬 《发光学报》 EI CAS CSCD 北大核心 1994年第3期253-256,共4页
本文报道了酶联免疫-化学发光法测定ANT的实验研究.兔抗ANT抗血清滴度1/500~1/104,ANT检测范围0~10μg/ml.
关键词 酶联免疫 ant 梯恩梯 发光免疫分析
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南极超微细菌ANT52(Alteromonas stellipolaris)外膜蛋白和脂多糖的提取物对黑鲷的免疫活性研究 被引量:10
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作者 王海丽 杨季芳 《海洋与湖沼》 CAS CSCD 北大核心 2009年第2期242-248,共7页
从南极超微细菌ANT52(Alteromonas stellipolaris)提取粗外膜蛋白(Outer membrane protein,OMP)和粗脂多糖(Lipopolysaccharide,LPS),分不同处理(OMP、LPS、OMP+LPS)免疫注射黑鲷,在免疫后的第1、7、14、21、28、35天时检测试验鱼的白... 从南极超微细菌ANT52(Alteromonas stellipolaris)提取粗外膜蛋白(Outer membrane protein,OMP)和粗脂多糖(Lipopolysaccharide,LPS),分不同处理(OMP、LPS、OMP+LPS)免疫注射黑鲷,在免疫后的第1、7、14、21、28、35天时检测试验鱼的白细胞吞噬活性、溶菌酶活力、抗菌活力以及酚氧化酶活力等非特异性免疫指标的变化。结果表明,自南极超微细菌ANT52提取的LPS及OMP均能够显著提高黑鲷的白细胞吞噬活性(P<0.05),增强黑鲷血清中的抗菌活力、溶菌酶的活力和酚氧化酶活力(P<0.05)。各免疫处理对黑鲷免疫活性的影响效果不同,LPS、OMP单独注射的免疫促进效果优于组合注射(LPS+OMP);且LPS、OMP单独注射的黑鲷各免疫指标基本上在接种后的第28天时最高,而组合注射LPS+OMP在接种后的第21天最高。上述物质表现的免疫作用机理有待于进一步研究。 展开更多
关键词 ant52(Alteromonas stellipolaris) 外膜蛋白 脂多糖 黑鲷 免疫活性
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NT4p53(N15)Ant重组腺病毒对肝癌细胞腹腔种植瘤的治疗作用 被引量:2
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作者 吴学元 白斌 +1 位作者 张党锋 马巍 《西安交通大学学报(医学版)》 CAS CSCD 北大核心 2014年第1期73-76,107,共5页
目的研究NT4p53(N15)Ant融合基因重组腺病毒预先感染ICR小鼠腹腔后,对肝癌细胞株H22腹腔种植瘤的治疗作用。方法将16只ICR小鼠随机平均分成2组,分别于腹腔内预先感染Ad-NT4p53(N15)Ant(治疗组)及等量空病毒(对照组)。将H22细胞悬液接种... 目的研究NT4p53(N15)Ant融合基因重组腺病毒预先感染ICR小鼠腹腔后,对肝癌细胞株H22腹腔种植瘤的治疗作用。方法将16只ICR小鼠随机平均分成2组,分别于腹腔内预先感染Ad-NT4p53(N15)Ant(治疗组)及等量空病毒(对照组)。将H22细胞悬液接种于小鼠腹腔,建立小鼠腹腔泛发性种植瘤模型。根据小鼠生长情况、生存期以及腹水瘤细胞形态、细胞凋亡率和小鼠腹腔内瘤体重量等结果,评价Ad-NT4p53(N15)Ant对H22细胞种植瘤及癌性腹水的治疗效果。结果成功构建了小鼠腹腔泛发性种植瘤动物模型。与对照组相比,Ad-NT4p53(N15)Ant组小鼠生存期延长、腹水的产生时间延迟,腹水中多核巨细胞减少,细胞凋亡及坏死的比率升高,腹腔内瘤体的质量减少。结论 Ad-NT4p53(15)Ant可有效抑制H22肝癌细胞腹腔移植瘤生长,延迟腹水形成,诱导体内腹水瘤细胞凋亡及坏死,延长小鼠生存期。 展开更多
关键词 NT4p53(N15)ant 重组腺相关病毒 肝癌 凋亡 癌转移 腹水
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A novel speech emotion recognition algorithm based on combination of emotion data field and ant colony search strategy 被引量:3
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作者 查诚 陶华伟 +3 位作者 张昕然 周琳 赵力 杨平 《Journal of Southeast University(English Edition)》 EI CAS 2016年第2期158-163,共6页
In order to effectively conduct emotion recognition from spontaneous, non-prototypical and unsegmented speech so as to create a more natural human-machine interaction; a novel speech emotion recognition algorithm base... In order to effectively conduct emotion recognition from spontaneous, non-prototypical and unsegmented speech so as to create a more natural human-machine interaction; a novel speech emotion recognition algorithm based on the combination of the emotional data field (EDF) and the ant colony search (ACS) strategy, called the EDF-ACS algorithm, is proposed. More specifically, the inter- relationship among the turn-based acoustic feature vectors of different labels are established by using the potential function in the EDF. To perform the spontaneous speech emotion recognition, the artificial colony is used to mimic the turn- based acoustic feature vectors. Then, the canonical ACS strategy is used to investigate the movement direction of each artificial ant in the EDF, which is regarded as the emotional label of the corresponding turn-based acoustic feature vector. The proposed EDF-ACS algorithm is evaluated on the continueous audio)'visual emotion challenge (AVEC) 2012 dataset, which contains the spontaneous, non-prototypical and unsegmented speech emotion data. The experimental results show that the proposed EDF-ACS algorithm outperforms the existing state-of-the-art algorithm in turn-based speech emotion recognition. 展开更多
关键词 speech emotion recognition emotional data field ant colony search human-machine interaction
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Antibacterial Effect of Waste Liquor of Essence Oil Extraction from Sabina vulgaris Ant in Foods 被引量:2
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作者 张海芳 石晓红 +2 位作者 王林和 张国盛 赵丽芹 《Agricultural Science & Technology》 CAS 2016年第2期414-416,共3页
The antibacterial effects of waste liquor of essence oil extraction from Sabina vulgaris Ant on Escherichia coil, Staphylococcus aureus, Salmonella, Bacillus subtilis, Bacillus magaterium and Bacillus cereus in foods ... The antibacterial effects of waste liquor of essence oil extraction from Sabina vulgaris Ant on Escherichia coil, Staphylococcus aureus, Salmonella, Bacillus subtilis, Bacillus magaterium and Bacillus cereus in foods were evaluated by paper disk method. The results showed that the waste liquor of essence oil extraction from S. vulgaris had inhibitory effects on E. coil, S. aureus and Salmonella with bet- ter inhibitory effects on E. coil and Salmonella; the bacteriostatic effect of water steam distillation waste liquor was better than that of water extraction waste liquor. The more times the waste liquor was distilled, the higher the active ingredients content was, and the better the bacteriostatic effect was. 展开更多
关键词 Sabina vulgaris ant Contamination bacteria Bacteriostatic effect
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Research on global path planning based on ant colony optimization for AUV 被引量:7
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作者 王宏健 熊伟 《Journal of Marine Science and Application》 2009年第1期58-64,共7页
Path planning is an important issue for autonomous underwater vehicles (AUVs) traversing an unknown environment such as a sea floor, a jungle, or the outer celestial planets. For this paper, global path planning usi... Path planning is an important issue for autonomous underwater vehicles (AUVs) traversing an unknown environment such as a sea floor, a jungle, or the outer celestial planets. For this paper, global path planning using large-scale chart data was studied, and the principles of ant colony optimization (ACO) were applied. This paper introduced the idea of a visibility graph based on the grid workspace model. It also brought a series of pheromone updating rules for the ACO planning algorithm. The operational steps of the ACO algorithm are proposed as a model for a global path planning method for AUV. To mimic the process of smoothing a planned path, a cutting operator and an insertion-point operator were designed. Simulation results demonstrated that the ACO algorithm is suitable for global path planning. The system has many advantages, including that the operating path of the AUV can be quickly optimized, and it is shorter, safer, and smoother. The prototype system successfully demonstrated the feasibility of the concept, proving it can be applied to surveys of unstructured unmanned environments. 展开更多
关键词 autonomous underwater vehicle (AUV) path planning ant colony optimization pathsmoothing
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NT4p53(N15)Ant融合基因重组腺病毒的构建及其体外抑瘤作用 被引量:1
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作者 宋丽萍 李跃萍 +3 位作者 邱曙东 黄辰 王爱英 李围围 《癌症》 SCIE CAS CSCD 北大核心 2008年第2期144-148,共5页
背景与目的:有研究发现P53氨基端15肽与穿膜肽(antennapedia,Ant)的融合肽能迅速穿透细胞膜,直接、快速引起乳腺癌、胰腺癌细胞坏死而非凋亡,但对正常细胞几乎没有毒性。本研究构建缺陷型腺病毒载体表达NT4p53(N15)Ant融合基因,研究其... 背景与目的:有研究发现P53氨基端15肽与穿膜肽(antennapedia,Ant)的融合肽能迅速穿透细胞膜,直接、快速引起乳腺癌、胰腺癌细胞坏死而非凋亡,但对正常细胞几乎没有毒性。本研究构建缺陷型腺病毒载体表达NT4p53(N15)Ant融合基因,研究其体外抑瘤作用。方法:应用Ad-EasyTM系统,在大肠杆菌同源重组,构建NT4p53(N15)Ant腺病毒表达载体,在HEK-293细胞内成功包装并鉴定后,感染肝癌细胞株HepG2,用倒置相差显微镜、透射电镜观察细胞形态学变化、四甲基偶氮唑蓝(MTT)比色实验及培养基中的乳酸脱氢酶(lactatedehydrogenase,LDH)测定研究其体外抑瘤作用。结果:Ad-NT4p53(N15)Ant对肝癌细胞株HepG2有明显抑制作用,48、72、96h细胞存活率分别为36.67%、20.47%、17.82%,与空病毒处理组比较差异有统计学意义(P<0.05);电镜观察到感染的HepG2细胞胞膜、核膜破坏,胞浆内出现囊泡状物质,染色质聚集。Ad-NT4p53(N15)Ant处理HepG2细胞24、48、72、96h时培养液中LDH释放分别为94、236、267、313U/L,较空病毒处理组明显增加,其差异有统计学意义(P<0.05)。结论:构建的腺病毒载体Ad-NT4p53(N15)Ant感染HepG2细胞后能抑制细胞增殖。 展开更多
关键词 NT4p53(N15)ant 腺病毒载体 肿瘤 基因治疗
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雷公藤内酯醇对佐剂性关节组织MIP-1α RANTES和VEGF表达影响的实验研究 被引量:2
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作者 韦登明 王一凡 +1 位作者 王琳 龙正海 《中华中医药学刊》 CAS 2009年第11期2413-2416,共4页
目的:探讨雷公藤内酯醇对佐剂性关节炎大鼠病变关节组织MIP-1α、RANTES和VEGF表达的影响。方法:在建立大鼠佐剂性关节炎模型基础上,给予不同剂量雷公藤内酯醇后,观察病变关节组织病理损害程度和MIP-1α、RANTES和VEGF免疫组织化学染色... 目的:探讨雷公藤内酯醇对佐剂性关节炎大鼠病变关节组织MIP-1α、RANTES和VEGF表达的影响。方法:在建立大鼠佐剂性关节炎模型基础上,给予不同剂量雷公藤内酯醇后,观察病变关节组织病理损害程度和MIP-1α、RANTES和VEGF免疫组织化学染色结果。结果:与模型组比较,3个雷公藤内酯醇治疗组关节肿胀度和病变关节组织MIP-1α、RANTES和VEGF表达明显减少,病变关节组织的病理损害明显改善。结论:雷公藤内酯醇可抑制佐剂性关节炎大鼠病变关节组织MIP-1α、RANTES和VEGF表达。 展开更多
关键词 雷公藤内酯醇 佐剂性关节炎 MIP-1α:[{antES VEGF
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