目的探究纤维蛋白原样蛋白1(fibrinogen-like protein 1,FGL1)和膜联蛋白A11(annexin A11,ANXA11)对肺癌手术患者效用评估价值及与预后的相关性。方法将本院2020年1月至2023年12月接诊的98例肺癌患者纳入肺癌组,另选取98例健康人作为对...目的探究纤维蛋白原样蛋白1(fibrinogen-like protein 1,FGL1)和膜联蛋白A11(annexin A11,ANXA11)对肺癌手术患者效用评估价值及与预后的相关性。方法将本院2020年1月至2023年12月接诊的98例肺癌患者纳入肺癌组,另选取98例健康人作为对照组。受试者入院后分别于空腹状态下采取6 mL肘静脉血,采用ELISA法检测血清ANXA11水平,采用实时荧光定量PCR技术检测ANXA11表达水平。根据术后1个月肿瘤标志物变化、影像学表现和免疫功能指标变化情况分为治疗有效组(n=68)和无效组(n=30)。患者出院后均行12~36个月的随访,根据患者预后情况分为预后良好组(n=63)和预后不良组(n=35)。对比对照组和肺癌组/有效组和无效组/预后FGL1、ANXA11表达差异;ROC分析FGL1、ANXA11单一及联合检测对肺癌手术患者临床效用评估价值;Spearman分析FGL1、ANXA11表达与预后的关系。结果肺癌组FGL1和ANXA11表达水平明显高于对照组(均P<0.05);无效组FGL1和ANXA11表达水平明显高于有效组(均P<0.05);ROC结果显示FGL1、ANXA11单独及联合检测对肺癌手术患者效用评估的曲线线下面积分别为0.928,并且联合检测具有较高的特异性(95.59%)以及敏感度(90.00%),诊断价值显著高于单独检测ROC曲线线下面积(均P<0.05);预后不良组FGL1和ANXA11表达水平明显高于预后良好组(均P<0.05);Spearman相关性结果显示FGL1、ANXA11与肺癌手术患者预后呈显著正相关(r=0.771、0.793,均P<0.05)。结论肺癌患者的FGL1与ANXA11表达水平高于健康人,治疗无效者高于有效者,预后不良者高于良好者,二者单独及联合检测对患者手术治疗效用评估均有价值,且其与预后相关性显著。展开更多
Background Annexin-1 was identified as an endometriosis-related protein by comparative proteomics in previous study. As an endogeneous anti-inflammatory mediator, Annexin-1 has been shown to regulate the immune respon...Background Annexin-1 was identified as an endometriosis-related protein by comparative proteomics in previous study. As an endogeneous anti-inflammatory mediator, Annexin-1 has been shown to regulate the immune response, cell proliferation and apoptosis. To investigate whether Annexin-1 is involved in the pathogenesis of endometriosis, we examined the expression of Annexin-1 in eutopic endometrium of women with or without endometriosis, and detected its expression in peritoneal fluids of those with endometriosis. Methods Eutopic endometrium samples from twenty-five women with endometriosis and those from sixteen age-matched women without endometriosis were collected. Peritoneal fluids were obtained from ten patients with endometriosis. The expression of Annexin-1 protein in eutopic endometrium was detected by immunohistochemistry and Western blotting, and mRNA detected by real-time PCR. Annexin-1 protein in the peritoneal fluids was detected by Western blotting.Results Annexin-1 mRNA and protein were overexpressed in eutopic endometrium of endometriosis without significant differences between the proliferative and secretory phase. Immunohistochemistry showed that Annexin-1 protein was expressed mainly in endometrial glandular celts throughout the menstrual cycle. Annexin-1 protein was detected in the peritoneal fluids of all the ten patients with endometriosis. Conclusions Annexin-1 is overexpressed in eutopic endometrium and presents in the peritoneal fluids of patients with endometriosis, and may play a role in the pathogenesis of endometriosis.展开更多
目的探究妊娠高血压(HDP)患者血清膜联蛋白A2(ANXA2)、血管生成抑制素-1(VASH1)和陷窝蛋白-1(CAV-1)在妊娠结局评估中的价值。方法选取2020年6月~2023年4月青岛妇女儿童医院收治的125例HDP患者为研究组,根据妊娠结局分为妊娠结局良好组(...目的探究妊娠高血压(HDP)患者血清膜联蛋白A2(ANXA2)、血管生成抑制素-1(VASH1)和陷窝蛋白-1(CAV-1)在妊娠结局评估中的价值。方法选取2020年6月~2023年4月青岛妇女儿童医院收治的125例HDP患者为研究组,根据妊娠结局分为妊娠结局良好组(n=77)和妊娠结局不良组(n=48),另选同期进行孕检的正常孕妇100例为对照组。采用酶联免疫吸附法(ELISA)检测血清ANXA2,VASH1和CAV-1水平;Logistic回归分析HDP孕妇妊娠结局的影响因素;绘制受试者工作特征(ROC)曲线分析血清ANXA2,VASH1和CAV-1对HDP患者妊娠结局的诊断价值;Pearson法分析血清ANXA2,VASH1,CAV-1与24h尿蛋白含量及血压指标相关性。结果与对照组相比,研究组血清ANXA2(353.19±37.31μg/L vs 415.32±42.65μg/L),CAV-1(6.05±0.64μg/L vs 6.89±0.73μg/L)水平显著降低(t=11.644,9.188),VASH1(615.48±62.46ng/L vs 528.63±54.39ng/L)水平显著升高(t=10.969),差异具有统计学意义(均P<0.05)。妊娠结局不良组较妊娠结局良好组血清ANXA2(322.54±34.24μg/L vs 372.29±39.23μg/L),CAV-1(5.56±0.59μg/L vs 6.38±0.67μg/L)水平显著降低(t=7.323,6.706),VASH1水平(660.29±67.34ng/L vs 587.56±59.42ng/L)显著升高(t=6.321),差异具有统计学意义(均P<0.05)。24h尿蛋白含量、VASH1为影响HDP患者不良妊娠结局的危险因素,血清ANXA2,CAV-1为影响HDP患者不良妊娠结局的保护因素,差异具有统计学意义(均P<0.05);ANXA2,VASH1,CAV-1以及三者联合预测HDP患者妊娠结局的曲线下面积(AUC)分别为0.809,0.747,0.775和0.909,三者联合预测的AUC显著优于ANXA2,VASH1和CAV-1单独预测(Z=2.720,3.334,3.320,均P<0.05);ANXA2,CAV-1与24h尿蛋白含量、收缩压、舒张压呈负相关(r=-0.435~-0.406,均P<0.05);VASH1与24h尿蛋白含量、收缩压、舒张压呈正相关(r=0.428,0.409,0.421,均P<0.05)。结论HDP患者的血清ANXA2,CAV-1水平降低,VASH1水平升高,对HDP诊断及妊娠结局评估具有一定辅助预测价值。展开更多
文摘目的探究纤维蛋白原样蛋白1(fibrinogen-like protein 1,FGL1)和膜联蛋白A11(annexin A11,ANXA11)对肺癌手术患者效用评估价值及与预后的相关性。方法将本院2020年1月至2023年12月接诊的98例肺癌患者纳入肺癌组,另选取98例健康人作为对照组。受试者入院后分别于空腹状态下采取6 mL肘静脉血,采用ELISA法检测血清ANXA11水平,采用实时荧光定量PCR技术检测ANXA11表达水平。根据术后1个月肿瘤标志物变化、影像学表现和免疫功能指标变化情况分为治疗有效组(n=68)和无效组(n=30)。患者出院后均行12~36个月的随访,根据患者预后情况分为预后良好组(n=63)和预后不良组(n=35)。对比对照组和肺癌组/有效组和无效组/预后FGL1、ANXA11表达差异;ROC分析FGL1、ANXA11单一及联合检测对肺癌手术患者临床效用评估价值;Spearman分析FGL1、ANXA11表达与预后的关系。结果肺癌组FGL1和ANXA11表达水平明显高于对照组(均P<0.05);无效组FGL1和ANXA11表达水平明显高于有效组(均P<0.05);ROC结果显示FGL1、ANXA11单独及联合检测对肺癌手术患者效用评估的曲线线下面积分别为0.928,并且联合检测具有较高的特异性(95.59%)以及敏感度(90.00%),诊断价值显著高于单独检测ROC曲线线下面积(均P<0.05);预后不良组FGL1和ANXA11表达水平明显高于预后良好组(均P<0.05);Spearman相关性结果显示FGL1、ANXA11与肺癌手术患者预后呈显著正相关(r=0.771、0.793,均P<0.05)。结论肺癌患者的FGL1与ANXA11表达水平高于健康人,治疗无效者高于有效者,预后不良者高于良好者,二者单独及联合检测对患者手术治疗效用评估均有价值,且其与预后相关性显著。
基金This work was supported by a grant from the National Natural Science Foundation of China （No. 30600669）.
文摘Background Annexin-1 was identified as an endometriosis-related protein by comparative proteomics in previous study. As an endogeneous anti-inflammatory mediator, Annexin-1 has been shown to regulate the immune response, cell proliferation and apoptosis. To investigate whether Annexin-1 is involved in the pathogenesis of endometriosis, we examined the expression of Annexin-1 in eutopic endometrium of women with or without endometriosis, and detected its expression in peritoneal fluids of those with endometriosis. Methods Eutopic endometrium samples from twenty-five women with endometriosis and those from sixteen age-matched women without endometriosis were collected. Peritoneal fluids were obtained from ten patients with endometriosis. The expression of Annexin-1 protein in eutopic endometrium was detected by immunohistochemistry and Western blotting, and mRNA detected by real-time PCR. Annexin-1 protein in the peritoneal fluids was detected by Western blotting.Results Annexin-1 mRNA and protein were overexpressed in eutopic endometrium of endometriosis without significant differences between the proliferative and secretory phase. Immunohistochemistry showed that Annexin-1 protein was expressed mainly in endometrial glandular celts throughout the menstrual cycle. Annexin-1 protein was detected in the peritoneal fluids of all the ten patients with endometriosis. Conclusions Annexin-1 is overexpressed in eutopic endometrium and presents in the peritoneal fluids of patients with endometriosis, and may play a role in the pathogenesis of endometriosis.
文摘目的探究妊娠高血压(HDP)患者血清膜联蛋白A2(ANXA2)、血管生成抑制素-1(VASH1)和陷窝蛋白-1(CAV-1)在妊娠结局评估中的价值。方法选取2020年6月~2023年4月青岛妇女儿童医院收治的125例HDP患者为研究组,根据妊娠结局分为妊娠结局良好组(n=77)和妊娠结局不良组(n=48),另选同期进行孕检的正常孕妇100例为对照组。采用酶联免疫吸附法(ELISA)检测血清ANXA2,VASH1和CAV-1水平;Logistic回归分析HDP孕妇妊娠结局的影响因素;绘制受试者工作特征(ROC)曲线分析血清ANXA2,VASH1和CAV-1对HDP患者妊娠结局的诊断价值;Pearson法分析血清ANXA2,VASH1,CAV-1与24h尿蛋白含量及血压指标相关性。结果与对照组相比,研究组血清ANXA2(353.19±37.31μg/L vs 415.32±42.65μg/L),CAV-1(6.05±0.64μg/L vs 6.89±0.73μg/L)水平显著降低(t=11.644,9.188),VASH1(615.48±62.46ng/L vs 528.63±54.39ng/L)水平显著升高(t=10.969),差异具有统计学意义(均P<0.05)。妊娠结局不良组较妊娠结局良好组血清ANXA2(322.54±34.24μg/L vs 372.29±39.23μg/L),CAV-1(5.56±0.59μg/L vs 6.38±0.67μg/L)水平显著降低(t=7.323,6.706),VASH1水平(660.29±67.34ng/L vs 587.56±59.42ng/L)显著升高(t=6.321),差异具有统计学意义(均P<0.05)。24h尿蛋白含量、VASH1为影响HDP患者不良妊娠结局的危险因素,血清ANXA2,CAV-1为影响HDP患者不良妊娠结局的保护因素,差异具有统计学意义(均P<0.05);ANXA2,VASH1,CAV-1以及三者联合预测HDP患者妊娠结局的曲线下面积(AUC)分别为0.809,0.747,0.775和0.909,三者联合预测的AUC显著优于ANXA2,VASH1和CAV-1单独预测(Z=2.720,3.334,3.320,均P<0.05);ANXA2,CAV-1与24h尿蛋白含量、收缩压、舒张压呈负相关(r=-0.435~-0.406,均P<0.05);VASH1与24h尿蛋白含量、收缩压、舒张压呈正相关(r=0.428,0.409,0.421,均P<0.05)。结论HDP患者的血清ANXA2,CAV-1水平降低,VASH1水平升高,对HDP诊断及妊娠结局评估具有一定辅助预测价值。
