AIM： To substantiate the claims of Unani and Arabian traditional medicine practitioners on the gastroprotective potential effect of a popular spice anise, ＂Pimpinella anisum L.＂ on experimentally-induced gastric ul...AIM： To substantiate the claims of Unani and Arabian traditional medicine practitioners on the gastroprotective potential effect of a popular spice anise, ＂Pimpinella anisum L.＂ on experimentally-induced gastric ulceration and secretion in rats. METHODS： Acute gastric ulceration in rats was produced by various noxious chemicals including 80% ethanol, 0.2 mol/L NaOH, 25% NaCI and indomethacin. Anti-secretory studies were undertaken using pylorusligated Shay rat technique. Levels of gastric non-protein sulfhydryls （NP-SH） and wall mucus were estimated and gastric tissue was also examined histologically. Anise aqueous suspension was used in two doses （250 and 500 mg/kg body weight） in all experiments. RESULTS： Anise significantly inhibited gastric mu- cosal damage induced by necrotizing agents and indomethacin. The anti-ulcer effect was further confirmed histologically. In pylorus-ligated Shay rats, anise suspension significantly reduced the basal gastric acid secretion, acidity and completely inhibited the rumenal ulceration. On the other hand, the suspension significantly replenished ethanol-induced depleted levels of gastric mucosal NP-SH and gastric wall mucus concentration. CONCLUSION： Anise aqueous suspension possesses significant cytoprotective and anti-ulcer activities against experimentallynduced gastric lesions. The anti-ulcer effect of anise is possibly prostaglandin-mediated and/orthrough its anti-secretory and antioxidative properties.展开更多
In the present study,we developed a rapid and specific reversed-phase high-performance liquid chromatographic(RP-HPLC)method for the quantification of p-hydroxyphenethyl anisate(HPA),one of the main bioactive constitu...In the present study,we developed a rapid and specific reversed-phase high-performance liquid chromatographic(RP-HPLC)method for the quantification of p-hydroxyphenethyl anisate(HPA),one of the main bioactive constituents of the roots and rhizomes of Notopterygium incisum and N.franchetii,in rat plasma after an intravenous(20 mg/kg,i.v.)and an intragastrical(200 mg/kg,i.g.)administration to rats,respectively.The method involved a plasma clear-up step using liquid-liquid extraction by EtOAc,followed by RP-HPLC separation and detection.Separation of HPA was performed on an analytical DiamonsilTM ODS C18 column with the mobile phase of MeOH-H2 O at ratios of 75:25(v/v)for i.v.and 70:30(v/v)for i.g.administration.The flow-rate was 1.0 mL/min,and UV detection was performed at 256 nm.The calibration curves were linear over the ranges of 0.05-5.0μg/mL(r2=0.9984)for i.v.and 0.5-10.0μg/mL(r2=0.9995)for i.g.administration in rat plasma.The extraction recoveries were in the range of82.01%-87.97%.The intra-and inter-day precisions were between 1.71%and 3.99%,with accuracies ranging from 91.22%to110.5%.The absolute bioavailability of an orally administered HPA in rats was about 48.17%.The developed method was suitable for the determination and pharmacokinetic study of HPA in rat plasma.展开更多
Aniseed distillates, like ouzo in Greece, raki in Turkey, pastis in France, and arak in Syria, are very popular in Mediterranean countries. This flavoured alcoholic beverage is produced by extraction and distillation ...Aniseed distillates, like ouzo in Greece, raki in Turkey, pastis in France, and arak in Syria, are very popular in Mediterranean countries. This flavoured alcoholic beverage is produced by extraction and distillation of fermented grape pomaces with herbal seeds from aromatic plants like Pimpinella anisum L., Foeniculum vulgate, Illicium verum and other plants which are also characterized by the presence of congeners which arise during fermentation process. Trans-anethol and its isomer cis-anethol are the main volatiles and are responsible for aroma properties of these traditional aniseed distillates. The aim of this research work was to find the concentration of special aromatic agent, trans-anethol and cis-anethol and also the major aromatic substances which are responsible for the aromatic quality. The majority of their identified components are isolated by extraction and distillation from different commercial brands and homemade samples. The constituents were identified by GC-MS （gas chromatography-mass spectometry） in this traditional spirit. The major constituents in distilled ouzo extract were also trans-anethol.展开更多
[Objectives] This study was conducted to isolate and screen the bacteria that can convert trans-anethole to anisic acid from star anise and its environmental samples, and identify the bacteria. [Methods] According to ...[Objectives] This study was conducted to isolate and screen the bacteria that can convert trans-anethole to anisic acid from star anise and its environmental samples, and identify the bacteria. [Methods] According to the traditional microbial culture method, with trans-anethole as the sole carbon source, through enrichment culture and separation and purification, preliminary screening by thin layer chromatography and re-screening by high-performance liquid chromatography, strains that degraded trans-anethole to produce anisic acid were obtained, and 16 S rDNA sequencing and phylogenetic tree construction were performed for genetic analysis. [Results] Eleven strains that degraded trans-anethole to produce anisic acid were obtained, among which strain NT2 that produced anisic acid with a relatively high efficiency was initially identified as Pseudomonas sp. The strain’s trans-anethole degradation rate was 45.41%, and the molar production rate and cumulative concentration of anisic acid were 21.80% and 1.96 g/L, respectively. [Conclusions] Strain NT2 has a strong ability to degrade trans-anethole to produce anisic acid, and can enrich strain resources for degradation of trans-anethole to anisic acid through microbial conversion.展开更多
This experiment was conducted at the poultry farm, poultry research station, state board of agriculture research, ministry of agriculture to study the effect of supplemental anise seed on broiler diets. A total of 640...This experiment was conducted at the poultry farm, poultry research station, state board of agriculture research, ministry of agriculture to study the effect of supplemental anise seed on broiler diets. A total of 640 of one-day old unsexed broiler chicks （Cobb） were used in this study. They were randomly distributed to four treatments. Anise seeds were supplemented at the levels of 0.2%, 0.4% and 0.6% compared with the control group for 49 days. The results showed： there was a significant （P 〈 0.05） increase in the rate of body weight for the two treatments 0.6% and 0.4% of the anise seeds compared with 0.2% and control treatments, body weight gain take the same direction, like in body weight, the highest treatment was 0.6% followed by treatment 0.4% as well as the feed consumption has increased in these treatments than in 0.2% and control treatments, so about feed conversion ratio the treatment 0.6% of the anise seeds recorded the best feed conversion ratio compared with other treatments. Significant decreased in mortality and significant increased in production index by increasing the level of anise seeds in feed. Dressing percentage increased significantly for all treatments using anise seeds and treatment 0.6% recorded highest dressing percentage, from the other hand percentage of abdominal fat decreased significantly by increasing anise seed levels and no significant difference in the percentages of liver, gizzard and heart were recorded in this study. About carcass cuts, the weights of primary cuts （thigh, drum stick, breast） relative to body weight recorded significant increase in the treatments using anise seeds at the expense of secondary cuts （neck, wings, back）. There was a significant decline （P 〈 0.05） for total bacterial （E. coli and Staphylococci） in small intestine in all level of anise seeds （0.2%, 0.4%, 0.6%） in ration while the decline of two types of bacteria in colon recorded in 0.4% and 0.6% treatments compared with 0.2~/0 and control treatments. Conclude from this study the possibility of using anise seeds at all levels used in the experiment and the best level that can be used to get the best performance of the positive results of broiler chickens is 0.6%.展开更多
Star anise(Illicium verum Hook.f.,SA)is a well-known culinary seasoning in China and Vietnam.Actually,SA also has been widely used as a traditional Chinese medicine in China with a long history.Phytochemical analysis ...Star anise(Illicium verum Hook.f.,SA)is a well-known culinary seasoning in China and Vietnam.Actually,SA also has been widely used as a traditional Chinese medicine in China with a long history.Phytochemical analysis has revealed that SA contains a high concentration of essential oils,phenols,flavonoids,and other bioactive compounds that contribute to its diverse pharmacological properties.These properties include antibacterial,anti-inflammatory and analgesic,anti-oxidation,antiviral,anti-cancer,anti-diabetes,antidiarrheal,and promoting hair growth.Various preclinical studies have shown that SA extracts and their active constituents may have potential therapeutic applications in preventing and treating various diseases.However,a comprehensive report on the relationship between the active ingredient,biological activity,and food characteristics of SA is rare.The medicinal value of SA has not been well valued and developed.This review provides an overview of the botanical chemistry and pharmacological properties of SA,as well as its potential innovative applications in food and personal care products,aiming to provide theoretical support for its further development and utilization.展开更多
Afghanistan is the battlefield where the U.S.army fought the longest overseas.Since U.S.President Joe Biden took office in January,the war’s future course has come in the spotlight.According to an agreement signed be...Afghanistan is the battlefield where the U.S.army fought the longest overseas.Since U.S.President Joe Biden took office in January,the war’s future course has come in the spotlight.According to an agreement signed between the Donald Trump administration and the Taliban in late February 2020,the U.S.展开更多
八角茴香(Illicium verum Hook.f.)属于木兰科植物,是中国特有的药食同源植物,目前研究集中于其化学成分与药理作用的开发利用。然而,八角茴香化学成分的系统鉴定和药理作用的定量数据不足,加工及提取技术缺乏统一规范。综述系统梳理了...八角茴香(Illicium verum Hook.f.)属于木兰科植物,是中国特有的药食同源植物,目前研究集中于其化学成分与药理作用的开发利用。然而,八角茴香化学成分的系统鉴定和药理作用的定量数据不足,加工及提取技术缺乏统一规范。综述系统梳理了八角茴香的主要成分,包括挥发油、黄酮类、酚酸类、倍半萜内酯类等,分析了其抗菌、镇痛、抗炎、抗氧化等药理作用,并总结了提取、分析及鲜料加工等方法的工艺要点,以期为完善八角茴香研究方法与工艺标准,提升资源利用效率和产业化应用提供有力支撑。展开更多
基金Supported by King AbdulAziz City for Science and Technology, Riyadh, Saudi Arabia, Spices Project No. AR1637
文摘AIM： To substantiate the claims of Unani and Arabian traditional medicine practitioners on the gastroprotective potential effect of a popular spice anise, ＂Pimpinella anisum L.＂ on experimentally-induced gastric ulceration and secretion in rats. METHODS： Acute gastric ulceration in rats was produced by various noxious chemicals including 80% ethanol, 0.2 mol/L NaOH, 25% NaCI and indomethacin. Anti-secretory studies were undertaken using pylorusligated Shay rat technique. Levels of gastric non-protein sulfhydryls （NP-SH） and wall mucus were estimated and gastric tissue was also examined histologically. Anise aqueous suspension was used in two doses （250 and 500 mg/kg body weight） in all experiments. RESULTS： Anise significantly inhibited gastric mu- cosal damage induced by necrotizing agents and indomethacin. The anti-ulcer effect was further confirmed histologically. In pylorus-ligated Shay rats, anise suspension significantly reduced the basal gastric acid secretion, acidity and completely inhibited the rumenal ulceration. On the other hand, the suspension significantly replenished ethanol-induced depleted levels of gastric mucosal NP-SH and gastric wall mucus concentration. CONCLUSION： Anise aqueous suspension possesses significant cytoprotective and anti-ulcer activities against experimentallynduced gastric lesions. The anti-ulcer effect of anise is possibly prostaglandin-mediated and/orthrough its anti-secretory and antioxidative properties.
基金The National Natural Science Foundation of China(Grant No.30672609)National High Technology Research and Development Program of China(Grant No.2002AA2Z343C,2004AA2Z3783)+1 种基金National Sciences and Technology Program of China(Grant No.2006BAI06A01-02)Beijing Municipal Special-Purpose Science Foundation of China(Grant No.Z0004105040311)。
文摘In the present study,we developed a rapid and specific reversed-phase high-performance liquid chromatographic(RP-HPLC)method for the quantification of p-hydroxyphenethyl anisate(HPA),one of the main bioactive constituents of the roots and rhizomes of Notopterygium incisum and N.franchetii,in rat plasma after an intravenous(20 mg/kg,i.v.)and an intragastrical(200 mg/kg,i.g.)administration to rats,respectively.The method involved a plasma clear-up step using liquid-liquid extraction by EtOAc,followed by RP-HPLC separation and detection.Separation of HPA was performed on an analytical DiamonsilTM ODS C18 column with the mobile phase of MeOH-H2 O at ratios of 75:25(v/v)for i.v.and 70:30(v/v)for i.g.administration.The flow-rate was 1.0 mL/min,and UV detection was performed at 256 nm.The calibration curves were linear over the ranges of 0.05-5.0μg/mL(r2=0.9984)for i.v.and 0.5-10.0μg/mL(r2=0.9995)for i.g.administration in rat plasma.The extraction recoveries were in the range of82.01%-87.97%.The intra-and inter-day precisions were between 1.71%and 3.99%,with accuracies ranging from 91.22%to110.5%.The absolute bioavailability of an orally administered HPA in rats was about 48.17%.The developed method was suitable for the determination and pharmacokinetic study of HPA in rat plasma.
文摘Aniseed distillates, like ouzo in Greece, raki in Turkey, pastis in France, and arak in Syria, are very popular in Mediterranean countries. This flavoured alcoholic beverage is produced by extraction and distillation of fermented grape pomaces with herbal seeds from aromatic plants like Pimpinella anisum L., Foeniculum vulgate, Illicium verum and other plants which are also characterized by the presence of congeners which arise during fermentation process. Trans-anethol and its isomer cis-anethol are the main volatiles and are responsible for aroma properties of these traditional aniseed distillates. The aim of this research work was to find the concentration of special aromatic agent, trans-anethol and cis-anethol and also the major aromatic substances which are responsible for the aromatic quality. The majority of their identified components are isolated by extraction and distillation from different commercial brands and homemade samples. The constituents were identified by GC-MS （gas chromatography-mass spectometry） in this traditional spirit. The major constituents in distilled ouzo extract were also trans-anethol.
基金Supported by The Basic Ability Improvement Project for Young and Middle-aged Teachers in Guangxi Universities(2017KY0288)。
文摘[Objectives] This study was conducted to isolate and screen the bacteria that can convert trans-anethole to anisic acid from star anise and its environmental samples, and identify the bacteria. [Methods] According to the traditional microbial culture method, with trans-anethole as the sole carbon source, through enrichment culture and separation and purification, preliminary screening by thin layer chromatography and re-screening by high-performance liquid chromatography, strains that degraded trans-anethole to produce anisic acid were obtained, and 16 S rDNA sequencing and phylogenetic tree construction were performed for genetic analysis. [Results] Eleven strains that degraded trans-anethole to produce anisic acid were obtained, among which strain NT2 that produced anisic acid with a relatively high efficiency was initially identified as Pseudomonas sp. The strain’s trans-anethole degradation rate was 45.41%, and the molar production rate and cumulative concentration of anisic acid were 21.80% and 1.96 g/L, respectively. [Conclusions] Strain NT2 has a strong ability to degrade trans-anethole to produce anisic acid, and can enrich strain resources for degradation of trans-anethole to anisic acid through microbial conversion.
文摘This experiment was conducted at the poultry farm, poultry research station, state board of agriculture research, ministry of agriculture to study the effect of supplemental anise seed on broiler diets. A total of 640 of one-day old unsexed broiler chicks （Cobb） were used in this study. They were randomly distributed to four treatments. Anise seeds were supplemented at the levels of 0.2%, 0.4% and 0.6% compared with the control group for 49 days. The results showed： there was a significant （P 〈 0.05） increase in the rate of body weight for the two treatments 0.6% and 0.4% of the anise seeds compared with 0.2% and control treatments, body weight gain take the same direction, like in body weight, the highest treatment was 0.6% followed by treatment 0.4% as well as the feed consumption has increased in these treatments than in 0.2% and control treatments, so about feed conversion ratio the treatment 0.6% of the anise seeds recorded the best feed conversion ratio compared with other treatments. Significant decreased in mortality and significant increased in production index by increasing the level of anise seeds in feed. Dressing percentage increased significantly for all treatments using anise seeds and treatment 0.6% recorded highest dressing percentage, from the other hand percentage of abdominal fat decreased significantly by increasing anise seed levels and no significant difference in the percentages of liver, gizzard and heart were recorded in this study. About carcass cuts, the weights of primary cuts （thigh, drum stick, breast） relative to body weight recorded significant increase in the treatments using anise seeds at the expense of secondary cuts （neck, wings, back）. There was a significant decline （P 〈 0.05） for total bacterial （E. coli and Staphylococci） in small intestine in all level of anise seeds （0.2%, 0.4%, 0.6%） in ration while the decline of two types of bacteria in colon recorded in 0.4% and 0.6% treatments compared with 0.2~/0 and control treatments. Conclude from this study the possibility of using anise seeds at all levels used in the experiment and the best level that can be used to get the best performance of the positive results of broiler chickens is 0.6%.
基金supported by the Guangxi Science and Technology Major Project(GUIKEAA22096029 and AA23023035)Macao Young Scholars Program(AM2022022).
文摘Star anise(Illicium verum Hook.f.,SA)is a well-known culinary seasoning in China and Vietnam.Actually,SA also has been widely used as a traditional Chinese medicine in China with a long history.Phytochemical analysis has revealed that SA contains a high concentration of essential oils,phenols,flavonoids,and other bioactive compounds that contribute to its diverse pharmacological properties.These properties include antibacterial,anti-inflammatory and analgesic,anti-oxidation,antiviral,anti-cancer,anti-diabetes,antidiarrheal,and promoting hair growth.Various preclinical studies have shown that SA extracts and their active constituents may have potential therapeutic applications in preventing and treating various diseases.However,a comprehensive report on the relationship between the active ingredient,biological activity,and food characteristics of SA is rare.The medicinal value of SA has not been well valued and developed.This review provides an overview of the botanical chemistry and pharmacological properties of SA,as well as its potential innovative applications in food and personal care products,aiming to provide theoretical support for its further development and utilization.
文摘Afghanistan is the battlefield where the U.S.army fought the longest overseas.Since U.S.President Joe Biden took office in January,the war’s future course has come in the spotlight.According to an agreement signed between the Donald Trump administration and the Taliban in late February 2020,the U.S.
文摘八角茴香(Illicium verum Hook.f.)属于木兰科植物,是中国特有的药食同源植物,目前研究集中于其化学成分与药理作用的开发利用。然而,八角茴香化学成分的系统鉴定和药理作用的定量数据不足,加工及提取技术缺乏统一规范。综述系统梳理了八角茴香的主要成分,包括挥发油、黄酮类、酚酸类、倍半萜内酯类等,分析了其抗菌、镇痛、抗炎、抗氧化等药理作用,并总结了提取、分析及鲜料加工等方法的工艺要点,以期为完善八角茴香研究方法与工艺标准,提升资源利用效率和产业化应用提供有力支撑。
