The application of generative artificial intelligence(AI)is bringing about notable changes in anime creation.This paper surveys recent advancements and applications of diffusion and language models in anime generation...The application of generative artificial intelligence(AI)is bringing about notable changes in anime creation.This paper surveys recent advancements and applications of diffusion and language models in anime generation,focusing on their demonstrated potential to enhance production efficiency through automation and personalization.Despite these benefits,it is crucial to acknowledge the substantial initial computational investments required for training and deploying these models.We conduct an in-depth survey of cutting-edge generative AI technologies,encompassing models such as Stable Diffusion and GPT,and appraise pivotal large-scale datasets alongside quantifiable evaluation metrics.Review of the surveyed literature indicates the achievement of considerable maturity in the capacity of AI models to synthesize high-quality,aesthetically compelling anime visual images from textual prompts,alongside discernible progress in the generation of coherent narratives.However,achieving perfect long-form consistency,mitigating artifacts like flickering in video sequences,and enabling fine-grained artistic control remain critical ongoing challenges.Building upon these advancements,research efforts have increasingly pivoted towards the synthesis of higher-dimensional content,such as video and three-dimensional assets,with recent studies demonstrating significant progress in this burgeoning field.Nevertheless,formidable challenges endure amidst these advancements.Foremost among these are the substantial computational exigencies requisite for training and deploying these sophisticated models,particularly pronounced in the realm of high-dimensional generation such as video synthesis.Additional persistent hurdles include maintaining spatial-temporal consistency across complex scenes and mitigating ethical considerations surrounding bias and the preservation of human creative autonomy.This research underscores the transformative potential and inherent complexities of AI-driven synergy within the creative industries.We posit that future research should be dedicated to the synergistic fusion of diffusion and autoregressive models,the integration of multimodal inputs,and the balanced consideration of ethical implications,particularly regarding bias and the preservation of human creative autonomy,thereby establishing a robust foundation for the advancement of anime creation and the broader landscape of AI-driven content generation.展开更多
The link between film/drama-induced tourism and the Internet to promote the destination apart from purchase intention has been studied for a long while. By using cinema hall and television, cost-efficient promotion to...The link between film/drama-induced tourism and the Internet to promote the destination apart from purchase intention has been studied for a long while. By using cinema hall and television, cost-efficient promotion tools were shaped. Anime consisting of digital drawing techniques has been one of the most remarkable examples in terms of promotion of destination and culture as well. In this paper, to evaluate the travel intention of watchers in Turkish anime forums to Japan, open ended questions based on the model of purchase intention were presented to members and followers in two popular anime forums via the Internet. The responses of 31 participants were analyzed. The results demonstrated that participants＇ purchase intention has been enhanced as the consequence of watching anime series.展开更多
The rise of the anirne subculture assembles the huge youth groups in the construction of the socialist core value system, and provides a new carrier and injected into the innovative dynamic. At the same time, their pe...The rise of the anirne subculture assembles the huge youth groups in the construction of the socialist core value system, and provides a new carrier and injected into the innovative dynamic. At the same time, their perverse behavior and exaggerated style of dress and the strange language constitute the alienation and challenges of a core value system. It should strengthen the ideological review and classification management, with the criticism of the mainstream media to enhance the vigilance of the community, as well as through commercial activities to digest rebel style, which is the effective way to promote the core value system.展开更多
Anime movie is a branch of cinematics.Unlike traditional movies,anime movies stand out with special characteristics in terms of how they shape the personality of the characters and how they portray the inner world of ...Anime movie is a branch of cinematics.Unlike traditional movies,anime movies stand out with special characteristics in terms of how they shape the personality of the characters and how they portray the inner world of those characters.“Realism”and“surrealism”are important elements in anime movies to portray the stories.In this article,the special aesthetic of anime movies is analyzed using the works produced by Hayao Miyazaki.The internal conflict between“realism”and“surrealism”is analyzed in order to understand the character image and story theme in an in-depth manner.展开更多
Cartoons serve as significant sources of entertainment for children and adolescents.However,numerous animated videos contain unsuitable content,such as violence,eroticism,abuse,and vehicular accidents.Current content ...Cartoons serve as significant sources of entertainment for children and adolescents.However,numerous animated videos contain unsuitable content,such as violence,eroticism,abuse,and vehicular accidents.Current content detection methods rely on manual inspection,which is resource-intensive,time-consuming,and not always reliable.Therefore,more efficient detection methods are necessary to safeguard young viewers.This paper addresses this significant problem by proposing a novel deep learning-based system,AnimeNet,designed to detect varying degrees of violent and erotic content in videos.AnimeNet utilizes a novel Convolutional Neural Network(CNN)model to extract image features effectively,classifying violent and erotic scenes in videos and images.The novelty of the work lies in the introduction of a novel channel-spatial attention module,enhancing the feature extraction performance of the CNN model,an advancement over previous efforts in the literature.To validate the approach,I compared AnimeNet with state-of-the-art classification methods,including ResNet,RegNet,ConvNext,ViT,and MobileNet.These were used to identify violent and erotic scenes within specific video frames.The results showed that AnimeNet outperformed these models,proving it to be well-suited for real-time applications in videos or images.This work presents a significant leap forward in automatic content detection in animation,offering a high-accuracy solution that is less resource-intensive and more reliable than current methods.The proposed approach enables it possible to better protect young audiences from exposure to unsuitable content,underlining its importance and potential for broad social impact.展开更多
Against the backdrop of internet globalization and digitalization,animations are widely spread worldwide as a means of entertainment.As an important channel to promote culture through digital media in the new era,anim...Against the backdrop of internet globalization and digitalization,animations are widely spread worldwide as a means of entertainment.As an important channel to promote culture through digital media in the new era,animations have become the key carrier to convey core values in cross-cultural communication.This study,based on existing literature,analyzes how anime disseminates culture through visuals and music.To achieve the research aim of this study,specific cases are selected to explore the impact of values conveyed in anime on the audience.Meanwhile,it examines how animations boost the local economy and promote cultural exchange.In the process of anime production,producers should not only maintain the richness and coherence of the storyline but also engage the audience's emotions.In addition,the culture intended to be promoted should be integrated into the details of the final work.This will facilitate the audience to reflect on and resonate with the cultural meaning while watching,thereby achieving a subtle and implicit effect of cultural dissemination.展开更多
This study takes the children’s animation IP“Brave Brother Ah Niu”as a case study,where the animation IP refers not merely to a single work,but rather to a core content asset capable of multi-dimensional developmen...This study takes the children’s animation IP“Brave Brother Ah Niu”as a case study,where the animation IP refers not merely to a single work,but rather to a core content asset capable of multi-dimensional development and possessing long-term commercial value,such as a well-known character,story,or worldview.This case is used to explore the path for children’s animation IP to empower the deep integration of cultural tourism based on Bourdieu’s field theory.In response to the current challenges of fragmentation,superficial symbolization,and capital feedback disruption in development,a three-field transformation framework of“cultural production-media practiceindustrial consumption”is constructed,and an integration mechanism of“empowerment-translation-feedback”is proposed.This study provides a replicable theoretical model and practical path for the Ah Niu IP and similar regional children’s animation IPs to overcome the bottleneck of cultural tourism integration,achieve cultural inheritance,and enhance the industrial value.展开更多
Traumatic brain injury can be categorized into primary and secondary injuries.Secondary injuries are the main cause of disability following traumatic brain injury,which involves a complex multicellular cascade.Microgl...Traumatic brain injury can be categorized into primary and secondary injuries.Secondary injuries are the main cause of disability following traumatic brain injury,which involves a complex multicellular cascade.Microglia play an important role in secondary injury and can be activated in response to traumatic brain injury.In this article,we review the origin and classification of microglia as well as the dynamic changes of microglia in traumatic brain injury.We also clarify the microglial polarization pathways and the therapeutic drugs targeting activated microglia.We found that regulating the signaling pathways involved in pro-inflammatory and anti-inflammatory microglia,such as the Toll-like receptor 4/nuclear factor-kappa B,mitogen-activated protein kinase,Janus kinase/signal transducer and activator of transcription,phosphoinositide 3-kinase/protein kinase B,Notch,and high mobility group box 1 pathways,can alleviate the inflammatory response triggered by microglia in traumatic brain injury,thereby exerting neuroprotective effects.We also reviewed the strategies developed on the basis of these pathways,such as drug and cell replacement therapies.Drugs that modulate inflammatory factors,such as rosuvastatin,have been shown to promote the polarization of antiinflammatory microglia and reduce the inflammatory response caused by traumatic brain injury.Mesenchymal stem cells possess anti-inflammatory properties,and clinical studies have confirmed their significant efficacy and safety in patients with traumatic brain injury.Additionally,advancements in mesenchymal stem cell-delivery methods—such as combinations of novel biomaterials,genetic engineering,and mesenchymal stem cell exosome therapy—have greatly enhanced the efficiency and therapeutic effects of mesenchymal stem cells in animal models.However,numerous challenges in the application of drug and mesenchymal stem cell treatment strategies remain to be addressed.In the future,new technologies,such as single-cell RNA sequencing and transcriptome analysis,can facilitate further experimental studies.Moreover,research involving non-human primates can help translate these treatment strategies to clinical practice.展开更多
Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food mater...Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.展开更多
A Novel Dosimetry Method for Small Animal Irradiators Using 3D-printed Mouse Phantoms and Alanine Dosimeters.Christopher Duncan1,Chad Gunther1(1.C&C Irradiator Service,LLC,Washington,DC,20006.)Abstract:Accurate do...A Novel Dosimetry Method for Small Animal Irradiators Using 3D-printed Mouse Phantoms and Alanine Dosimeters.Christopher Duncan1,Chad Gunther1(1.C&C Irradiator Service,LLC,Washington,DC,20006.)Abstract:Accurate dosimetry is a crucial component of small animal and preclinical irradiation studies.展开更多
The automatic colorization of anime line drawings is a challenging problem in production pipelines.Recent advances in deep neural networks have addressed this problem;however,collectingmany images of colorization targ...The automatic colorization of anime line drawings is a challenging problem in production pipelines.Recent advances in deep neural networks have addressed this problem;however,collectingmany images of colorization targets in novel anime work before the colorization process starts leads to chicken-and-egg problems and has become an obstacle to using them in production pipelines.To overcome this obstacle,we propose a new patch-based learning method for few-shot anime-style colorization.The learning method adopts an efficient patch sampling technique with position embedding according to the characteristics of anime line drawings.We also present a continuous learning strategy that continuously updates our colorization model using new samples colorized by human artists.The advantage of our method is that it can learn our colorization model from scratch or pre-trained weights using only a few pre-and post-colorized line drawings that are created by artists in their usual colorization work.Therefore,our method can be easily incorporated within existing production pipelines.We quantitatively demonstrate that our colorizationmethod outperforms state-of-the-art methods.展开更多
Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food mater...Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.展开更多
Background:The development of relevant and robust large animal models of hepatocellular carcinoma is needed to test new therapeutic strategies for this disease.Transgenic approaches hold promise in addressing this com...Background:The development of relevant and robust large animal models of hepatocellular carcinoma is needed to test new therapeutic strategies for this disease.Transgenic approaches hold promise in addressing this complex problem.One such model,the Oncopig,has been reported to develop tumors of up to 4 cm in diameter within 7-14 days at sites of in situ vector inoculation.However,the resulting lesions reportedly contained an extensive inflammatory component that has not been evaluated in detail.Methods:Herein,we describe our results from multiparametric characterization of the lesions generated using liver biopsy cores incubated in vector solution and re-placed in the tissue.The study consisted of 3 animals in 3 cohorts(total of 9 animals)that were evaluated at 14,21,and 28 days.CT imaging,immunohistochemistry,multiplex immunofluorescence,and comprehensive blood analyses were used to quantify composition of the hepatic masses that developed following AdCre inoculation.Results:The tumors were hypovascular on CT and predominantly composed of CD45+cells with a strong lymphohistiocytic component,with no carcinomas identified.Ki-67 staining showed proliferation of CD45+immune cells but no neoplastic component.To provide further insight,the results are evaluated in the context of tumor growth kinetics.Conclusion:While progress has been made in generating targetable lesions,achieving a robust large animal model of liver cancer that faithfully recapitulates the human disease remains a challenging goal.展开更多
On the occasion of the New Year,I would like to extend my sincere gratitude and New Year greetings to the experts,scholars,author teams,and readers who have long supported the development of Animal Models and Experime...On the occasion of the New Year,I would like to extend my sincere gratitude and New Year greetings to the experts,scholars,author teams,and readers who have long supported the development of Animal Models and Experimental Medicine(AMEM).Over the past year,we have faced challenges together and achieved breakthroughs in academic influence,internationalization,and fulfilling social respon-sibilities.Looking ahead,we are filled with confidence as we strive to build an important bridge connecting laboratory animal science and technology with academic research.展开更多
Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food mater...Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.The research scope includes the quality and processing characteristics of food raw materials,the relationships of nutritional components and bioactive substances with human health,product flavor and sensory characteristics,the control of harmful substances during processing or cooking,product preservation,storage and packaging;microorganisms and fermentation,illegal drug residues and food safety detection;authenticity identification;cell-cultured meat,regulations and standards.展开更多
Artificial light at night(ALAN),as an emerging pollutant,disrupts wild animals'nocturnal behaviours and physiological processes.Recent evidence indicates that ALAN can also impair diurnal cognition,especially in h...Artificial light at night(ALAN),as an emerging pollutant,disrupts wild animals'nocturnal behaviours and physiological processes.Recent evidence indicates that ALAN can also impair diurnal cognition,especially in highly developed vertebrates.However,previous research has rendered mixed results across taxa and task types,with the parameters of the light source also scattered.That limits conclusion generalizability.Here we examined cognitive impacts of ALAN in housed Java Sparrows(Lonchura oryzivora),focusing on two questions:(1)whether ALAN uniformly impairs diverse cognitive traits and(2)how correlated colour temperatures(CCTs)modulate these effects.Sparrows were exposed to amber light(low CCT),neutral-white light(medium CCT),blue light(high CCT),or a no-light control.We then compared individual performance in three cognitive paradigms which were used to assess the animals'capacities of discrimination learning,reversal learning,and inhibitory control.Results showed no significant effects of ALAN on discrimination learning,but ALAN-exposed birds required fewer trials in reversal learning.Lower CCT(amber light)led to more failures in detour-reaching.These findings indicate that cognitive impacts of ALAN are not uniformly negative but depend on cognitive function and CCT.Our study highlights context-dependent effects of ALAN,providing insights for optimizing urban lighting policies to balance ecological and human needs.展开更多
Pathological scarring,manifested in the form of hypertrophic scars(HTS)and keloid scars(KS),represents a major clinical challenge due to its aesthetic and functional implications for patients.Understanding the molecul...Pathological scarring,manifested in the form of hypertrophic scars(HTS)and keloid scars(KS),represents a major clinical challenge due to its aesthetic and functional implications for patients.Understanding the molecular mechanisms involved in these types of scars and developing effective treatments requires the use of controlled ex-perimental models,especially animals,to overcome the limitations of clinical studies.The aim of this sistematic review is to critically analyze the animal models used in the last five years(2020-2025)for the study of pathological scars,highlighting their advantages,limitations and applicability in the development of new therapeutic strat-egies.Murine,rabbit and porcine models,as well as alternative models,offer varied perspectives on the formation and treatment of HTS and KS,with an emphasis on histological and molecular correlations with human pathology.By synthesizing recent data,the paper highlights the essential role of preclinical research in optimizing an-tifibrotic treatments and in advancing the translation of data into the clinical sphere.Overall,animal models remain essential for bridging mechanistic insights with clinical translation,supporting the development of more effective and personalized anti-scar therapies.展开更多
Background:The aim of the study was to develop a non-human primate model of metabolic dysfunction in Macaca fascicularis using chronic high-fat diet(HFD)to mimic clinical disease progression.Methods:Thirty-five male m...Background:The aim of the study was to develop a non-human primate model of metabolic dysfunction in Macaca fascicularis using chronic high-fat diet(HFD)to mimic clinical disease progression.Methods:Thirty-five male macaques aged 10-15 years underwent an 18-month HFD intervention.Physiological parameters(BMI,BP,hematology),liver fat fraction(evaluated by ultrasound/MRI),cardiac function(assessed by echocardiography),and histopathology(using liver biopsy)were measured before and after the intervention.Serum proteomics with KEGG/STRING analyses identified molecular mechanisms.Results:Within 6 months,HFD induced dyslipidemia(elevated TG,TCHO,HDL-C,LDL-C).After 18 months,metabolic dysfunction-associated steatohepatitis(MASH)was confirmed by histopathology in 57.14%(16/28)of macaques,diabetes(elevated FPG/HbA1c)in 17.86%(5/28),and myocardial hypertrophy(elevated LVMass/LAD)in 46.43%(13/28).Proteomics identified Bile acid-CoA:amino acid N-acyltransferase(BAAT)as a MASH hallmark protein,the level of which was inversely correlated with the degree of fibrosis.For diabetes,citrate synthase(CS)and malate dehydrogenase 1(MDH1)impaired glucose oxidation via the TCA cycle,while hexose-6-phosphate de-hydrogenase(H6PD)disrupted gluconeogenesis.Myocardial hypertrophy was associ-ated with the downregulation of SRC proto-oncogene,non-receptor tyrosine kinase(SRC),mitogen-activated protein kinase 14(MAPK14),emerin(EMD),and integrin subunit beta 1(ITGB1).Conclusions:An 18-month HFD successfully established a translational M.fascicula-ris model replicating key metabolic disorders(MASH,diabetes,cardiac hypertrophy).BAAT,CS/MDH1/H6PD,and SRC/MAPK14/EMD/ITGB1 were identified as mecha-nistic biomarkers for these conditions.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.62202210).
文摘The application of generative artificial intelligence(AI)is bringing about notable changes in anime creation.This paper surveys recent advancements and applications of diffusion and language models in anime generation,focusing on their demonstrated potential to enhance production efficiency through automation and personalization.Despite these benefits,it is crucial to acknowledge the substantial initial computational investments required for training and deploying these models.We conduct an in-depth survey of cutting-edge generative AI technologies,encompassing models such as Stable Diffusion and GPT,and appraise pivotal large-scale datasets alongside quantifiable evaluation metrics.Review of the surveyed literature indicates the achievement of considerable maturity in the capacity of AI models to synthesize high-quality,aesthetically compelling anime visual images from textual prompts,alongside discernible progress in the generation of coherent narratives.However,achieving perfect long-form consistency,mitigating artifacts like flickering in video sequences,and enabling fine-grained artistic control remain critical ongoing challenges.Building upon these advancements,research efforts have increasingly pivoted towards the synthesis of higher-dimensional content,such as video and three-dimensional assets,with recent studies demonstrating significant progress in this burgeoning field.Nevertheless,formidable challenges endure amidst these advancements.Foremost among these are the substantial computational exigencies requisite for training and deploying these sophisticated models,particularly pronounced in the realm of high-dimensional generation such as video synthesis.Additional persistent hurdles include maintaining spatial-temporal consistency across complex scenes and mitigating ethical considerations surrounding bias and the preservation of human creative autonomy.This research underscores the transformative potential and inherent complexities of AI-driven synergy within the creative industries.We posit that future research should be dedicated to the synergistic fusion of diffusion and autoregressive models,the integration of multimodal inputs,and the balanced consideration of ethical implications,particularly regarding bias and the preservation of human creative autonomy,thereby establishing a robust foundation for the advancement of anime creation and the broader landscape of AI-driven content generation.
文摘The link between film/drama-induced tourism and the Internet to promote the destination apart from purchase intention has been studied for a long while. By using cinema hall and television, cost-efficient promotion tools were shaped. Anime consisting of digital drawing techniques has been one of the most remarkable examples in terms of promotion of destination and culture as well. In this paper, to evaluate the travel intention of watchers in Turkish anime forums to Japan, open ended questions based on the model of purchase intention were presented to members and followers in two popular anime forums via the Internet. The responses of 31 participants were analyzed. The results demonstrated that participants＇ purchase intention has been enhanced as the consequence of watching anime series.
文摘The rise of the anirne subculture assembles the huge youth groups in the construction of the socialist core value system, and provides a new carrier and injected into the innovative dynamic. At the same time, their perverse behavior and exaggerated style of dress and the strange language constitute the alienation and challenges of a core value system. It should strengthen the ideological review and classification management, with the criticism of the mainstream media to enhance the vigilance of the community, as well as through commercial activities to digest rebel style, which is the effective way to promote the core value system.
文摘Anime movie is a branch of cinematics.Unlike traditional movies,anime movies stand out with special characteristics in terms of how they shape the personality of the characters and how they portray the inner world of those characters.“Realism”and“surrealism”are important elements in anime movies to portray the stories.In this article,the special aesthetic of anime movies is analyzed using the works produced by Hayao Miyazaki.The internal conflict between“realism”and“surrealism”is analyzed in order to understand the character image and story theme in an in-depth manner.
文摘Cartoons serve as significant sources of entertainment for children and adolescents.However,numerous animated videos contain unsuitable content,such as violence,eroticism,abuse,and vehicular accidents.Current content detection methods rely on manual inspection,which is resource-intensive,time-consuming,and not always reliable.Therefore,more efficient detection methods are necessary to safeguard young viewers.This paper addresses this significant problem by proposing a novel deep learning-based system,AnimeNet,designed to detect varying degrees of violent and erotic content in videos.AnimeNet utilizes a novel Convolutional Neural Network(CNN)model to extract image features effectively,classifying violent and erotic scenes in videos and images.The novelty of the work lies in the introduction of a novel channel-spatial attention module,enhancing the feature extraction performance of the CNN model,an advancement over previous efforts in the literature.To validate the approach,I compared AnimeNet with state-of-the-art classification methods,including ResNet,RegNet,ConvNext,ViT,and MobileNet.These were used to identify violent and erotic scenes within specific video frames.The results showed that AnimeNet outperformed these models,proving it to be well-suited for real-time applications in videos or images.This work presents a significant leap forward in automatic content detection in animation,offering a high-accuracy solution that is less resource-intensive and more reliable than current methods.The proposed approach enables it possible to better protect young audiences from exposure to unsuitable content,underlining its importance and potential for broad social impact.
文摘Against the backdrop of internet globalization and digitalization,animations are widely spread worldwide as a means of entertainment.As an important channel to promote culture through digital media in the new era,animations have become the key carrier to convey core values in cross-cultural communication.This study,based on existing literature,analyzes how anime disseminates culture through visuals and music.To achieve the research aim of this study,specific cases are selected to explore the impact of values conveyed in anime on the audience.Meanwhile,it examines how animations boost the local economy and promote cultural exchange.In the process of anime production,producers should not only maintain the richness and coherence of the storyline but also engage the audience's emotions.In addition,the culture intended to be promoted should be integrated into the details of the final work.This will facilitate the audience to reflect on and resonate with the cultural meaning while watching,thereby achieving a subtle and implicit effect of cultural dissemination.
基金The 2023 Basic Scientific Research Ability Improvement Project for Young and Middle-aged Teachers in Guangxi Universities(2023KY1884)。
文摘This study takes the children’s animation IP“Brave Brother Ah Niu”as a case study,where the animation IP refers not merely to a single work,but rather to a core content asset capable of multi-dimensional development and possessing long-term commercial value,such as a well-known character,story,or worldview.This case is used to explore the path for children’s animation IP to empower the deep integration of cultural tourism based on Bourdieu’s field theory.In response to the current challenges of fragmentation,superficial symbolization,and capital feedback disruption in development,a three-field transformation framework of“cultural production-media practiceindustrial consumption”is constructed,and an integration mechanism of“empowerment-translation-feedback”is proposed.This study provides a replicable theoretical model and practical path for the Ah Niu IP and similar regional children’s animation IPs to overcome the bottleneck of cultural tourism integration,achieve cultural inheritance,and enhance the industrial value.
基金supported by the Natural Science Foundation of Yunnan Province,No.202401AS070086(to ZW)the National Key Research and Development Program of China,No.2018YFA0801403(to ZW)+1 种基金Yunnan Science and Technology Talent and Platform Plan,No.202105AC160041(to ZW)the Natural Science Foundation of China,No.31960120(to ZW)。
文摘Traumatic brain injury can be categorized into primary and secondary injuries.Secondary injuries are the main cause of disability following traumatic brain injury,which involves a complex multicellular cascade.Microglia play an important role in secondary injury and can be activated in response to traumatic brain injury.In this article,we review the origin and classification of microglia as well as the dynamic changes of microglia in traumatic brain injury.We also clarify the microglial polarization pathways and the therapeutic drugs targeting activated microglia.We found that regulating the signaling pathways involved in pro-inflammatory and anti-inflammatory microglia,such as the Toll-like receptor 4/nuclear factor-kappa B,mitogen-activated protein kinase,Janus kinase/signal transducer and activator of transcription,phosphoinositide 3-kinase/protein kinase B,Notch,and high mobility group box 1 pathways,can alleviate the inflammatory response triggered by microglia in traumatic brain injury,thereby exerting neuroprotective effects.We also reviewed the strategies developed on the basis of these pathways,such as drug and cell replacement therapies.Drugs that modulate inflammatory factors,such as rosuvastatin,have been shown to promote the polarization of antiinflammatory microglia and reduce the inflammatory response caused by traumatic brain injury.Mesenchymal stem cells possess anti-inflammatory properties,and clinical studies have confirmed their significant efficacy and safety in patients with traumatic brain injury.Additionally,advancements in mesenchymal stem cell-delivery methods—such as combinations of novel biomaterials,genetic engineering,and mesenchymal stem cell exosome therapy—have greatly enhanced the efficiency and therapeutic effects of mesenchymal stem cells in animal models.However,numerous challenges in the application of drug and mesenchymal stem cell treatment strategies remain to be addressed.In the future,new technologies,such as single-cell RNA sequencing and transcriptome analysis,can facilitate further experimental studies.Moreover,research involving non-human primates can help translate these treatment strategies to clinical practice.
文摘Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.
文摘A Novel Dosimetry Method for Small Animal Irradiators Using 3D-printed Mouse Phantoms and Alanine Dosimeters.Christopher Duncan1,Chad Gunther1(1.C&C Irradiator Service,LLC,Washington,DC,20006.)Abstract:Accurate dosimetry is a crucial component of small animal and preclinical irradiation studies.
文摘The automatic colorization of anime line drawings is a challenging problem in production pipelines.Recent advances in deep neural networks have addressed this problem;however,collectingmany images of colorization targets in novel anime work before the colorization process starts leads to chicken-and-egg problems and has become an obstacle to using them in production pipelines.To overcome this obstacle,we propose a new patch-based learning method for few-shot anime-style colorization.The learning method adopts an efficient patch sampling technique with position embedding according to the characteristics of anime line drawings.We also present a continuous learning strategy that continuously updates our colorization model using new samples colorized by human artists.The advantage of our method is that it can learn our colorization model from scratch or pre-trained weights using only a few pre-and post-colorized line drawings that are created by artists in their usual colorization work.Therefore,our method can be easily incorporated within existing production pipelines.We quantitatively demonstrate that our colorizationmethod outperforms state-of-the-art methods.
文摘Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.
基金Institutional Research Grant,MD Anderson Cancer CenterUPWARDS Training Program(Undergraduate Students Working Towards Research in Science),Grant/Award Number:1R25CA240137-01A1the CPRIT Research Training Award CPRIT Training Program,Grant/Award Number:RP210028。
文摘Background:The development of relevant and robust large animal models of hepatocellular carcinoma is needed to test new therapeutic strategies for this disease.Transgenic approaches hold promise in addressing this complex problem.One such model,the Oncopig,has been reported to develop tumors of up to 4 cm in diameter within 7-14 days at sites of in situ vector inoculation.However,the resulting lesions reportedly contained an extensive inflammatory component that has not been evaluated in detail.Methods:Herein,we describe our results from multiparametric characterization of the lesions generated using liver biopsy cores incubated in vector solution and re-placed in the tissue.The study consisted of 3 animals in 3 cohorts(total of 9 animals)that were evaluated at 14,21,and 28 days.CT imaging,immunohistochemistry,multiplex immunofluorescence,and comprehensive blood analyses were used to quantify composition of the hepatic masses that developed following AdCre inoculation.Results:The tumors were hypovascular on CT and predominantly composed of CD45+cells with a strong lymphohistiocytic component,with no carcinomas identified.Ki-67 staining showed proliferation of CD45+immune cells but no neoplastic component.To provide further insight,the results are evaluated in the context of tumor growth kinetics.Conclusion:While progress has been made in generating targetable lesions,achieving a robust large animal model of liver cancer that faithfully recapitulates the human disease remains a challenging goal.
文摘On the occasion of the New Year,I would like to extend my sincere gratitude and New Year greetings to the experts,scholars,author teams,and readers who have long supported the development of Animal Models and Experimental Medicine(AMEM).Over the past year,we have faced challenges together and achieved breakthroughs in academic influence,internationalization,and fulfilling social respon-sibilities.Looking ahead,we are filled with confidence as we strive to build an important bridge connecting laboratory animal science and technology with academic research.
文摘Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.The research scope includes the quality and processing characteristics of food raw materials,the relationships of nutritional components and bioactive substances with human health,product flavor and sensory characteristics,the control of harmful substances during processing or cooking,product preservation,storage and packaging;microorganisms and fermentation,illegal drug residues and food safety detection;authenticity identification;cell-cultured meat,regulations and standards.
基金supported by National Key Research and Development Project(2022YFC3202104)Tencent Foundation’s Biodiversity Conservation Funding Project for Universities。
文摘Artificial light at night(ALAN),as an emerging pollutant,disrupts wild animals'nocturnal behaviours and physiological processes.Recent evidence indicates that ALAN can also impair diurnal cognition,especially in highly developed vertebrates.However,previous research has rendered mixed results across taxa and task types,with the parameters of the light source also scattered.That limits conclusion generalizability.Here we examined cognitive impacts of ALAN in housed Java Sparrows(Lonchura oryzivora),focusing on two questions:(1)whether ALAN uniformly impairs diverse cognitive traits and(2)how correlated colour temperatures(CCTs)modulate these effects.Sparrows were exposed to amber light(low CCT),neutral-white light(medium CCT),blue light(high CCT),or a no-light control.We then compared individual performance in three cognitive paradigms which were used to assess the animals'capacities of discrimination learning,reversal learning,and inhibitory control.Results showed no significant effects of ALAN on discrimination learning,but ALAN-exposed birds required fewer trials in reversal learning.Lower CCT(amber light)led to more failures in detour-reaching.These findings indicate that cognitive impacts of ALAN are not uniformly negative but depend on cognitive function and CCT.Our study highlights context-dependent effects of ALAN,providing insights for optimizing urban lighting policies to balance ecological and human needs.
基金Ministry of Research,Innovation and Digitization,CCCDI-UEFISCDI,Grant/Award Number:PN-IV-P7-7.1-PED-2024-1578,within PNCDI Ⅳ.
文摘Pathological scarring,manifested in the form of hypertrophic scars(HTS)and keloid scars(KS),represents a major clinical challenge due to its aesthetic and functional implications for patients.Understanding the molecular mechanisms involved in these types of scars and developing effective treatments requires the use of controlled ex-perimental models,especially animals,to overcome the limitations of clinical studies.The aim of this sistematic review is to critically analyze the animal models used in the last five years(2020-2025)for the study of pathological scars,highlighting their advantages,limitations and applicability in the development of new therapeutic strat-egies.Murine,rabbit and porcine models,as well as alternative models,offer varied perspectives on the formation and treatment of HTS and KS,with an emphasis on histological and molecular correlations with human pathology.By synthesizing recent data,the paper highlights the essential role of preclinical research in optimizing an-tifibrotic treatments and in advancing the translation of data into the clinical sphere.Overall,animal models remain essential for bridging mechanistic insights with clinical translation,supporting the development of more effective and personalized anti-scar therapies.
基金National Key Research and Development Program of China,Grant/Award Number:2021YFF0702200Science and Technology Projects in Guangzhou,Grant/Award Number:202206010084,202206010197 and 202206060002+1 种基金Guangdong S&T programme,Grant/Award Number:2009A081000002 and 2023B0303040004Technology Planning Project of Linzhi,Grant/Award Number:2023-YZ-01。
文摘Background:The aim of the study was to develop a non-human primate model of metabolic dysfunction in Macaca fascicularis using chronic high-fat diet(HFD)to mimic clinical disease progression.Methods:Thirty-five male macaques aged 10-15 years underwent an 18-month HFD intervention.Physiological parameters(BMI,BP,hematology),liver fat fraction(evaluated by ultrasound/MRI),cardiac function(assessed by echocardiography),and histopathology(using liver biopsy)were measured before and after the intervention.Serum proteomics with KEGG/STRING analyses identified molecular mechanisms.Results:Within 6 months,HFD induced dyslipidemia(elevated TG,TCHO,HDL-C,LDL-C).After 18 months,metabolic dysfunction-associated steatohepatitis(MASH)was confirmed by histopathology in 57.14%(16/28)of macaques,diabetes(elevated FPG/HbA1c)in 17.86%(5/28),and myocardial hypertrophy(elevated LVMass/LAD)in 46.43%(13/28).Proteomics identified Bile acid-CoA:amino acid N-acyltransferase(BAAT)as a MASH hallmark protein,the level of which was inversely correlated with the degree of fibrosis.For diabetes,citrate synthase(CS)and malate dehydrogenase 1(MDH1)impaired glucose oxidation via the TCA cycle,while hexose-6-phosphate de-hydrogenase(H6PD)disrupted gluconeogenesis.Myocardial hypertrophy was associ-ated with the downregulation of SRC proto-oncogene,non-receptor tyrosine kinase(SRC),mitogen-activated protein kinase 14(MAPK14),emerin(EMD),and integrin subunit beta 1(ITGB1).Conclusions:An 18-month HFD successfully established a translational M.fascicula-ris model replicating key metabolic disorders(MASH,diabetes,cardiac hypertrophy).BAAT,CS/MDH1/H6PD,and SRC/MAPK14/EMD/ITGB1 were identified as mecha-nistic biomarkers for these conditions.
