The virtual technique in Chinese drama makes the aesthetic feeling of the audiences enriched in enjoying the art of stage performance to their heart＇s content when the reality of life is reflected by drama. Also, the...The virtual technique in Chinese drama makes the aesthetic feeling of the audiences enriched in enjoying the art of stage performance to their heart＇s content when the reality of life is reflected by drama. Also, the position of the animation art has been promoted by the expression and extension of the drama art spirit and expression technique in the creation of film and television animations to be greatly improved. In this paper, the characteristics of the virtual technique in drama are briefly introduced, and also the influence of the virtual technique on the creation offihn and television animations is analyzed.展开更多
The three-dimensional (3D) interactive animations and video games are so attractive that successful educational programming environments like Alice and Kodu Game Lab deal with 3D characters. Islay 3D is another educat...The three-dimensional (3D) interactive animations and video games are so attractive that successful educational programming environments like Alice and Kodu Game Lab deal with 3D characters. Islay 3D is another educational programming environment of which feature is an intuitively comprehensive user interface in terms of state transition diagrams. Unfortunately its animation definition was too memory-hungry when played by the built-in interpreter. In this paper, we present a translator of the animation definitions of Islay 3D into ActionScript3 (AS3). Compiling the AS3 codes by way of Papervision3D, we obtain the 3D Flash animation file playable on the Flash platform. It will be shown that the memory and CPU usages will be much saved, quartered and halved, respectively, by virtue of the translator.展开更多
Animal behavior researchers often face problems regarding standardization and reproducibility oftheir experiments. This has led to the partial substitution of live animals with artificial virtual stim-uli. In addition...Animal behavior researchers often face problems regarding standardization and reproducibility oftheir experiments. This has led to the partial substitution of live animals with artificial virtual stim-uli. In addition to standardization and reproducibility, virtual stimuli open new options for re-searchers since they are easily changeable in morphology and appearance, and their behavior canbe defined. In this article, a novel toolchain to conduct behavior experiments with fish is presentedby a case study in sailfin mollies Poecilia latipinna. As the toolchain holds many different and novelfeatures, it offers new possibilities for studies in behavioral animal research and promotes thestandardization of experiments. The presented method includes options to design, animate, andpresent virtual stimuli to live fish. The designing tool offers an easy and user-friendly way to definesize, coloration, and morphology of stimuli and moreover it is able to configure virtual stimuli ran-domly without any user influence. Furthermore, the toolchain brings a novel method to animatestimuli in a semiautomatic way with the help of a game controller. These created swimming pathscan be applied to different stimuli in real time. A presentation tool combines models and swim-ming paths regarding formerly defined playlists, and presents the stimuli onto 2 screens.Experiments with live sailfin mollies validated the usage of the created virtual 3D fish models inmate-choice experiments.展开更多
Visual signals, including changes in coloration and color patterns, are frequently used by animalsto convey information. During contests, body coloration and its changes can be used to assess anopponent＇s state or mo...Visual signals, including changes in coloration and color patterns, are frequently used by animalsto convey information. During contests, body coloration and its changes can be used to assess anopponent＇s state or motivation. Communication of aggressive propensity is particularly importantin group-living animals with a stable dominance hierarchy, as the outcome of aggressive inter-actions determines the social rank of group members. Neolamprologus pulcher is a cooperativelybreeding cichlid showing frequent within-group aggression. Both sexes exhibit two vertical blackstripes on the operculum that vary naturally in shape and darkness. During frontal threat displaysthese patterns are actively exposed to the opponent, suggesting a signaling function. To investi-gate the role of operculum stripes during contests we manipulated their darkness in computeranimated pictures of the fish. We recorded the responses in behavior and stripe darkness of testsubjects to which these animated pictures were presented. Individuals with initially darker stripeswere more aggressive against the animations and showed more operculum threat displays.Operculum stripes of test subjects became darker after exposure to an animation exhibiting a paleoperculum than after exposure to a dark operculum animation, highlighting the role of the dark-ness of this color pattern in opponent assessment. We conclude that （i） the black stripes on theoperculum of N. pulcherare a reliable signal of aggression and dominance, （ii） these markings playan important role in opponent assessment, and （iii） 2D computer animations are well suited to elicitbiologically meaningful short-term aggressive responses in this widely used model system ofsocial evolution.展开更多
The use of computer animation in behavioral research is a state-of-the-art method for designing andpresenting animated animals to live test animals. The major advantages of computer animations are：（1） the creation ...The use of computer animation in behavioral research is a state-of-the-art method for designing andpresenting animated animals to live test animals. The major advantages of computer animations are：（1） the creation of animated animal stimuli with high variability of morphology and even behavior; （2）animated stimuli provide highly standardized, controlled and repeatable testing procedures; and （3）they allow a reduction in the number of live test animals regarding the 3Rs principle. But the use of ani-mated animals should be attended by a thorough validation for each test species to verify that behaviormeasured with live animals toward virtual animals can also be expected with natural stimuli. Here wepresent results on the validation of a custom-made simulation for animated 3D sailfin mollies Poecilialatipinna and show that responses of live test females were as strong to an animated fish as to a videoor a live male fish. Movement of an animated stimulus was important but female response was stron-ger toward a swimming 3D fish stimulus than to a ＂swimming＂ box. Moreover, male test fish wereable to discriminate between animated male and female stimuli; hence, rendering the animated 3D fisha useful tool in mate-choice experiments with sailfin mollies.展开更多
The use of artificial stimuli in tests of animal behavior are common,and as technological advances increase,opportunities for the use of 3D computer animations in behavioral tests are becoming increasingly available(W...The use of artificial stimuli in tests of animal behavior are common,and as technological advances increase,opportunities for the use of 3D computer animations in behavioral tests are becoming increasingly available(Witte et al.2017).The importance of easy-tomanipulate,consistent,and reliably performing stimuli is clear,and this is readily achievable with the use of computer animations.Validation of animated stimuli is critical,however,to ensure that behaviors observed in response to artificial,computer-generated stimuli are equivalent to what would be expected if animals were presented with a comparable live stimuli(Woo and Rieucau 2011;Chouinard-Thuly et al.2017;Powell and Rosenthal 2017).展开更多
Cities serve as windows into human civilization,key spatial nodes for national communication,and carriers and repositories of rich and colorful Chinese stories.How to tell cities'stories well,and thus tell China&#...Cities serve as windows into human civilization,key spatial nodes for national communication,and carriers and repositories of rich and colorful Chinese stories.How to tell cities'stories well,and thus tell China's stories well?In Shandong Province,documentaries and animations have been used to break cultural barriers and open new horizons in city-fo cused international communication.展开更多
Traumatic brain injury can be categorized into primary and secondary injuries.Secondary injuries are the main cause of disability following traumatic brain injury,which involves a complex multicellular cascade.Microgl...Traumatic brain injury can be categorized into primary and secondary injuries.Secondary injuries are the main cause of disability following traumatic brain injury,which involves a complex multicellular cascade.Microglia play an important role in secondary injury and can be activated in response to traumatic brain injury.In this article,we review the origin and classification of microglia as well as the dynamic changes of microglia in traumatic brain injury.We also clarify the microglial polarization pathways and the therapeutic drugs targeting activated microglia.We found that regulating the signaling pathways involved in pro-inflammatory and anti-inflammatory microglia,such as the Toll-like receptor 4/nuclear factor-kappa B,mitogen-activated protein kinase,Janus kinase/signal transducer and activator of transcription,phosphoinositide 3-kinase/protein kinase B,Notch,and high mobility group box 1 pathways,can alleviate the inflammatory response triggered by microglia in traumatic brain injury,thereby exerting neuroprotective effects.We also reviewed the strategies developed on the basis of these pathways,such as drug and cell replacement therapies.Drugs that modulate inflammatory factors,such as rosuvastatin,have been shown to promote the polarization of antiinflammatory microglia and reduce the inflammatory response caused by traumatic brain injury.Mesenchymal stem cells possess anti-inflammatory properties,and clinical studies have confirmed their significant efficacy and safety in patients with traumatic brain injury.Additionally,advancements in mesenchymal stem cell-delivery methods—such as combinations of novel biomaterials,genetic engineering,and mesenchymal stem cell exosome therapy—have greatly enhanced the efficiency and therapeutic effects of mesenchymal stem cells in animal models.However,numerous challenges in the application of drug and mesenchymal stem cell treatment strategies remain to be addressed.In the future,new technologies,such as single-cell RNA sequencing and transcriptome analysis,can facilitate further experimental studies.Moreover,research involving non-human primates can help translate these treatment strategies to clinical practice.展开更多
Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food mater...Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.展开更多
BACKGROUND Non-erosive reflux disease(NERD),the main gastroesophageal reflux subtype,features reflux symptoms without mucosal damage.Anxiety links to visceral hypersensitivity in NERD,yet mechanisms and animal models ...BACKGROUND Non-erosive reflux disease(NERD),the main gastroesophageal reflux subtype,features reflux symptoms without mucosal damage.Anxiety links to visceral hypersensitivity in NERD,yet mechanisms and animal models are unclear.AIM To establish a translational NERD rat model with anxiety comorbidity via tail clamping and study corticotropin-releasing hormone(CRH)-mediated neuroimmune pathways in visceral hypersensitivity and esophageal injury.METHODS Sprague-Dawley(SD)and Wistar rats were grouped into sham,model,and modified groups(n=10 each).The treatments for the modified groups were as follows:SD rats received ovalbumin/aluminum hydroxide suspension+acid perfusion±tail clamping(40 minutes/day for 7 days),while Wistar rats received fructose water+tail clamping.Esophageal pathology,visceral sensitivity,and behavior were assessed.Serum CRH,calcitonin gene-related peptide(CGRP),5-hydroxytryptamine(5-HT),and mast cell tryptase(MCT)and central amygdala(CeA)CRH mRNA were measured via ELISA and qRT-PCR.RESULTS Tail clamping induced anxiety,worsening visceral hypersensitivity(lower abdominal withdrawal reflex thresholds,P<0.05)and esophageal injury(dilated intercellular spaces and mitochondrial edema).Both models showed raised serum CRH,CGRP,5-HT,and MCT(P<0.01)and CeA CRH mRNA expression(P<0.01).Behavioral tests confirmed anxiety-like phenotypes.NERD-anxiety rats showed clinical-like symptom severity without erosion.CONCLUSION Tail clamping induces anxiety in NERD models,worsening visceral hypersensitivity via CRH neuroimmune dysregulation,offering a translational model and highlighting CRH as a treatment target.展开更多
Effective treatment methods for stroke,a common cerebrovascular disease with a high mortality rate,are still being sought.Exosome therapy,a form of acellular therapy,has demonstrated promising efficacy in various dise...Effective treatment methods for stroke,a common cerebrovascular disease with a high mortality rate,are still being sought.Exosome therapy,a form of acellular therapy,has demonstrated promising efficacy in various diseases in animal models;however,there is currently insufficient evidence to guide the clinical application of exosome in patients with stroke.This article reviews the progress of exosome applications in stroke treatment.It aims to elucidate the significant potential value of exosomes in stroke therapy and provide a reference for their clinical translation.At present,many studies on exosome-based therapies for stroke are actively underway.Regarding preclinical research,exosomes,as bioactive substances with diverse sources,currently favor stem cells as their origin.Due to their high plasticity,exosomes can be effectively modified through various physical,chemical,and genetic engineering methods to enhance their efficacy.In animal models of stroke,exosome therapy can reduce neuroinflammatory responses,alleviate oxidative stress damage,and inhibit programmed cell death.Additionally,exosomes can promote angiogenesis,repair and regenerate damaged white matter fiber bundles,and facilitate the migration and differentiation of neural stem cells,aiding the repair process.We also summarize new directions for the application of exosomes,specifically the exosome intervention through the ventricular-meningeal lymphatic system.The review findings suggest that the treatment paradigm for stroke is poised for transformation.展开更多
Although previous studies have demonstrated that transcranial focused ultrasound stimulation protects the ischemic brain,clear criteria for the stimulation time window and intensity are lacking.Electrical impedance to...Although previous studies have demonstrated that transcranial focused ultrasound stimulation protects the ischemic brain,clear criteria for the stimulation time window and intensity are lacking.Electrical impedance tomography enables real-time monitoring of changes in cerebral blood perfusion within the ischemic brain,but investigating the feasibility of using this method to assess post-stroke rehabilitation in vivo remains critical.In this study,ischemic stroke was induced in rats through middle cerebral artery occlusion surgery.Transcranial focused ultrasound stimulation was used to treat the rat model of ischemia,and electrical impedance tomography was used to measure impedance during both the acute stage of ischemia and the rehabilitation stage following the stimulation.Electrical impedance tomography results indicated that cerebral impedance increased after the onset of ischemia and decreased following transcranial focused ultrasound stimulation.Furthermore,the stimulation promoted motor function recovery,reduced cerebral infarction volume in the rat model of ischemic stroke,and induced the expression of brain-derived neurotrophic factor in the ischemic brain.Our results also revealed a significant correlation between the impedance of the ischemic brain post-intervention and improvements in behavioral scores and infarct volume.This study shows that daily administration of transcranial focused ultrasound stimulation for 20 minutes to the ischemic hemisphere 24 hours after cerebral ischemia enhanced motor recovery in a rat model of ischemia.Additionally,our findings indicate that electrical impedance tomography can serve as a valuable tool for quantitatively evaluating rehabilitation after ischemic stroke in vivo.These findings suggest the feasibility of using impedance data collected via electrical impedance tomography to clinically assess the effects of rehabilitatory interventions for patients with ischemic stroke.展开更多
Myasthenia gravis is a chronic autoimmune disorder that affects the neuromuscular junction leading to fluctuating skeletal muscle fatigability. The majority of myasthenia gravis patients have detectable antibodies in ...Myasthenia gravis is a chronic autoimmune disorder that affects the neuromuscular junction leading to fluctuating skeletal muscle fatigability. The majority of myasthenia gravis patients have detectable antibodies in their serum, targeting acetylcholine receptor, muscle-specific kinase, or related proteins. Current treatment for myasthenia gravis involves symptomatic therapy, immunosuppressive drugs such as corticosteroids, azathioprine, and mycophenolate mofetil, and thymectomy, which is primarily indicated in patients with thymoma or thymic hyperplasia. However, this condition continues to pose significant challenges including an unpredictable and variable disease progression, differing response to individual therapies, and substantial longterm side effects associated with standard treatments(including an increased risk of infections, osteoporosis, and diabetes), underscoring the necessity for a more personalized approach to treatment. Furthermore, about fifteen percent of patients, called “refractory myasthenia gravis patients”, do not respond adequately to standard therapies. In this context, the introduction of molecular therapies has marked a significant advance in myasthenia gravis management. Advances in understanding myasthenia gravis pathogenesis, especially the role of pathogenic antibodies, have driven the development of these biological drugs, which offer more selective, rapid, and safer alternatives to traditional immunosuppressants. This review aims to provide a comprehensive overview of emerging therapeutic strategies targeting specific immune pathways in myasthenia gravis, with a particular focus on preclinical evidence, therapeutic rationale, and clinical translation of B-cell depletion therapies, neonatal Fc receptor inhibitors, and complement inhibitors.展开更多
Purpose The paper shows the interpretive impact of different constructions of the point of view available to the reader/viewer in book and animated movie versions of a children's picture book,a novel for pre-adole...Purpose The paper shows the interpretive impact of different constructions of the point of view available to the reader/viewer in book and animated movie versions of a children's picture book,a novel for pre-adolescents/early teenagers,and a graphic novel for adolescents and adults.Design/Approach/Methods Excerpts from book and animated movie versions of the same story are compared using multimodal analysis of interpersonal meaning to show how the reader/viewer is positioned in relation to the characters in each version,complemented by analyses of ideational meaning to show the effect of point of view on interpretive possibilities.Findings Focusing mainly on multimodal construction of point of view,the analyses show how interpretive possibilities of ostensibly the same story are significantly reconfigured in animated adaptations compared with book versions even when the verbal narrative remains substantially unchanged.Originality/Value The study shows that it is crucial to students’critical appreciation of,and their creative contribution to,their evolving digital literary culture that in this new era of educational technology,attention in literacy and literary education focuses on developing understandings of digital multimodal narrative art,and that animated movie adaptations are not presented pedagogically as isomorphic with,or simply adjunct to,corresponding book versions.展开更多
Data-driven garment animation is a current topic of interest in the computer graphics industry.Existing approaches generally establish the mapping between a single human pose or a temporal pose sequence,and garment de...Data-driven garment animation is a current topic of interest in the computer graphics industry.Existing approaches generally establish the mapping between a single human pose or a temporal pose sequence,and garment deformation,but it is difficult to quickly generate diverse clothed human animations.We address this problem with a method to automatically synthesize dressed human animations with temporal consistency from a specified human motion label.At the heart of our method is a twostage strategy.Specifically,we first learn a latent space encoding the sequence-level distribution of human motions utilizing a transformer-based conditional variational autoencoder(Transformer-CVAE).Then a garment simulator synthesizes dynamic garment shapes using a transformer encoder-decoder architecture.Since the learned latent space comes from varied human motions,our method can generate a variety of styles of motions given a specific motion label.By means of a novel beginning of sequence(BOS)learning strategy and a self-supervised refinement procedure,our garment simulator is capable of efficiently synthesizing garment deformation sequences corresponding to the generated human motions while maintaining temporal and spatial consistency.We verify our ideas experimentally.This is the first generative model that directly dresses human animation.展开更多
Major depressive disorder(MDD)affects people all over the world,and yet,its etiology is complex and remains incompletely understood.In this review,we aim to assess recent advances in understanding depression and its r...Major depressive disorder(MDD)affects people all over the world,and yet,its etiology is complex and remains incompletely understood.In this review,we aim to assess recent advances in understanding depression and its regulation,as well as its interaction with circadian rhythms.Circadian rhythms are internalized representations of the periodic daily light and dark cycles.Accumulating evidence has shown that MDD and the related mental disorders are associated with disrupted circadian rhythms.In particular,depression has often been linked to abnormalities in circadian rhythms because dysregulation of the circadian system increases susceptibility to MDD.The fact that several rhythms are disrupted in depressed patients suggests that these disruptions are not restricted to any one rhythm but rather involve the molecular circadian clock core machinery.The sleep-wake cycle is one rhythm that is often disrupted in depression,which often leads to disturbances in other rhythms.The circadian disruptions manifested in depressed patients and the effectiveness and fast action of chronobiologically based treatments highlight the circadian system as a key therapeutic target in the treatment of depression.This review assesses the evidence on rising depression rates and examines their contributing factors,including circadian misalignment.We discuss key hypotheses underlying depression pathogenesis,potential etiology,and relevant animal models,and underscore potential mechanisms driving depression's growing burden and how understanding these factors is critical for improving prevention and treatment strategies.展开更多
Depressive disorder is a chronic,recurring,and potentially life-endangering neuropsychiatric disease.According to a report by the World Health Organization,the global population suffering from depression is experienci...Depressive disorder is a chronic,recurring,and potentially life-endangering neuropsychiatric disease.According to a report by the World Health Organization,the global population suffering from depression is experiencing a significant annual increase.Despite its prevalence and considerable impact on people,little is known about its pathogenesis.One major reason is the scarcity of reliable animal models due to the absence of consensus on the pathology and etiology of depression.Furthermore,the neural circuit mechanism of depression induced by various factors is particularly complex.Considering the variability in depressive behavior patterns and neurobiological mechanisms among different animal models of depression,a comparison between the neural circuits of depression induced by various factors is essential for its treatment.In this review,we mainly summarize the most widely used behavioral animal models and neural circuits under different triggers of depression,aiming to provide a theoretical basis for depression prevention.展开更多
Rotavirus infections cause severe gastroenteritis and dehydration in young children and animals worldwide,leading to high rates of morbidity and mortality,predominantly in low-and middleincome countries.In the past de...Rotavirus infections cause severe gastroenteritis and dehydration in young children and animals worldwide,leading to high rates of morbidity and mortality,predominantly in low-and middleincome countries.In the past decade,substantial progress has been made in the development and implementation of rotavirus vaccines,which have been essential in alleviating the global burden of this disease,not only in human being but also in livestock species like calves and piglets,where these infections can cause significant economic losses.By synthesizing the latest research and real-world evidence,this review article is designated to provide deep insights into the current state of rotavirus vaccine technology and its global implementation as well as the application of rotavirus vaccines in veterinary settings and their importance in controlling zoonotic transmission and maintaining food security.展开更多
文摘The virtual technique in Chinese drama makes the aesthetic feeling of the audiences enriched in enjoying the art of stage performance to their heart＇s content when the reality of life is reflected by drama. Also, the position of the animation art has been promoted by the expression and extension of the drama art spirit and expression technique in the creation of film and television animations to be greatly improved. In this paper, the characteristics of the virtual technique in drama are briefly introduced, and also the influence of the virtual technique on the creation offihn and television animations is analyzed.
文摘The three-dimensional (3D) interactive animations and video games are so attractive that successful educational programming environments like Alice and Kodu Game Lab deal with 3D characters. Islay 3D is another educational programming environment of which feature is an intuitively comprehensive user interface in terms of state transition diagrams. Unfortunately its animation definition was too memory-hungry when played by the built-in interpreter. In this paper, we present a translator of the animation definitions of Islay 3D into ActionScript3 (AS3). Compiling the AS3 codes by way of Papervision3D, we obtain the 3D Flash animation file playable on the Flash platform. It will be shown that the memory and CPU usages will be much saved, quartered and halved, respectively, by virtue of the translator.
文摘Animal behavior researchers often face problems regarding standardization and reproducibility oftheir experiments. This has led to the partial substitution of live animals with artificial virtual stim-uli. In addition to standardization and reproducibility, virtual stimuli open new options for re-searchers since they are easily changeable in morphology and appearance, and their behavior canbe defined. In this article, a novel toolchain to conduct behavior experiments with fish is presentedby a case study in sailfin mollies Poecilia latipinna. As the toolchain holds many different and novelfeatures, it offers new possibilities for studies in behavioral animal research and promotes thestandardization of experiments. The presented method includes options to design, animate, andpresent virtual stimuli to live fish. The designing tool offers an easy and user-friendly way to definesize, coloration, and morphology of stimuli and moreover it is able to configure virtual stimuli ran-domly without any user influence. Furthermore, the toolchain brings a novel method to animatestimuli in a semiautomatic way with the help of a game controller. These created swimming pathscan be applied to different stimuli in real time. A presentation tool combines models and swim-ming paths regarding formerly defined playlists, and presents the stimuli onto 2 screens.Experiments with live sailfin mollies validated the usage of the created virtual 3D fish models inmate-choice experiments.
文摘Visual signals, including changes in coloration and color patterns, are frequently used by animalsto convey information. During contests, body coloration and its changes can be used to assess anopponent＇s state or motivation. Communication of aggressive propensity is particularly importantin group-living animals with a stable dominance hierarchy, as the outcome of aggressive inter-actions determines the social rank of group members. Neolamprologus pulcher is a cooperativelybreeding cichlid showing frequent within-group aggression. Both sexes exhibit two vertical blackstripes on the operculum that vary naturally in shape and darkness. During frontal threat displaysthese patterns are actively exposed to the opponent, suggesting a signaling function. To investi-gate the role of operculum stripes during contests we manipulated their darkness in computeranimated pictures of the fish. We recorded the responses in behavior and stripe darkness of testsubjects to which these animated pictures were presented. Individuals with initially darker stripeswere more aggressive against the animations and showed more operculum threat displays.Operculum stripes of test subjects became darker after exposure to an animation exhibiting a paleoperculum than after exposure to a dark operculum animation, highlighting the role of the dark-ness of this color pattern in opponent assessment. We conclude that （i） the black stripes on theoperculum of N. pulcherare a reliable signal of aggression and dominance, （ii） these markings playan important role in opponent assessment, and （iii） 2D computer animations are well suited to elicitbiologically meaningful short-term aggressive responses in this widely used model system ofsocial evolution.
文摘The use of computer animation in behavioral research is a state-of-the-art method for designing andpresenting animated animals to live test animals. The major advantages of computer animations are：（1） the creation of animated animal stimuli with high variability of morphology and even behavior; （2）animated stimuli provide highly standardized, controlled and repeatable testing procedures; and （3）they allow a reduction in the number of live test animals regarding the 3Rs principle. But the use of ani-mated animals should be attended by a thorough validation for each test species to verify that behaviormeasured with live animals toward virtual animals can also be expected with natural stimuli. Here wepresent results on the validation of a custom-made simulation for animated 3D sailfin mollies Poecilialatipinna and show that responses of live test females were as strong to an animated fish as to a videoor a live male fish. Movement of an animated stimulus was important but female response was stron-ger toward a swimming 3D fish stimulus than to a ＂swimming＂ box. Moreover, male test fish wereable to discriminate between animated male and female stimuli; hence, rendering the animated 3D fisha useful tool in mate-choice experiments with sailfin mollies.
文摘The use of artificial stimuli in tests of animal behavior are common,and as technological advances increase,opportunities for the use of 3D computer animations in behavioral tests are becoming increasingly available(Witte et al.2017).The importance of easy-tomanipulate,consistent,and reliably performing stimuli is clear,and this is readily achievable with the use of computer animations.Validation of animated stimuli is critical,however,to ensure that behaviors observed in response to artificial,computer-generated stimuli are equivalent to what would be expected if animals were presented with a comparable live stimuli(Woo and Rieucau 2011;Chouinard-Thuly et al.2017;Powell and Rosenthal 2017).
文摘Cities serve as windows into human civilization,key spatial nodes for national communication,and carriers and repositories of rich and colorful Chinese stories.How to tell cities'stories well,and thus tell China's stories well?In Shandong Province,documentaries and animations have been used to break cultural barriers and open new horizons in city-fo cused international communication.
基金supported by the Natural Science Foundation of Yunnan Province,No.202401AS070086(to ZW)the National Key Research and Development Program of China,No.2018YFA0801403(to ZW)+1 种基金Yunnan Science and Technology Talent and Platform Plan,No.202105AC160041(to ZW)the Natural Science Foundation of China,No.31960120(to ZW)。
文摘Traumatic brain injury can be categorized into primary and secondary injuries.Secondary injuries are the main cause of disability following traumatic brain injury,which involves a complex multicellular cascade.Microglia play an important role in secondary injury and can be activated in response to traumatic brain injury.In this article,we review the origin and classification of microglia as well as the dynamic changes of microglia in traumatic brain injury.We also clarify the microglial polarization pathways and the therapeutic drugs targeting activated microglia.We found that regulating the signaling pathways involved in pro-inflammatory and anti-inflammatory microglia,such as the Toll-like receptor 4/nuclear factor-kappa B,mitogen-activated protein kinase,Janus kinase/signal transducer and activator of transcription,phosphoinositide 3-kinase/protein kinase B,Notch,and high mobility group box 1 pathways,can alleviate the inflammatory response triggered by microglia in traumatic brain injury,thereby exerting neuroprotective effects.We also reviewed the strategies developed on the basis of these pathways,such as drug and cell replacement therapies.Drugs that modulate inflammatory factors,such as rosuvastatin,have been shown to promote the polarization of antiinflammatory microglia and reduce the inflammatory response caused by traumatic brain injury.Mesenchymal stem cells possess anti-inflammatory properties,and clinical studies have confirmed their significant efficacy and safety in patients with traumatic brain injury.Additionally,advancements in mesenchymal stem cell-delivery methods—such as combinations of novel biomaterials,genetic engineering,and mesenchymal stem cell exosome therapy—have greatly enhanced the efficiency and therapeutic effects of mesenchymal stem cells in animal models.However,numerous challenges in the application of drug and mesenchymal stem cell treatment strategies remain to be addressed.In the future,new technologies,such as single-cell RNA sequencing and transcriptome analysis,can facilitate further experimental studies.Moreover,research involving non-human primates can help translate these treatment strategies to clinical practice.
文摘Food Science of Animal Products(ISSN:2958-4124,e-ISSN:2958-3780)is a peer-reviewed,open access international journal that publishes the latest research findings in the field of animal-origin foods,involving food materials such as meat,aquatic products,milk,eggs,animal offals and edible insects.
基金Supported by the National Key Specialty of Traditional Chinese Medicine(Spleen and Stomach Diseases),No.0500004National Natural Science Foundation of China,No.82205104 and No.82104850+1 种基金Hospital Capability Enhancement Project of Xiyuan Hospital,CACMS,No.XYZX0303-07the Fundamental Research Funds for the Central Public Welfare Research Institutes,Excellent Young Scientists Training Program of China Academy of Chinese Medical Sciences,No.ZZ16-YQ-002.
文摘BACKGROUND Non-erosive reflux disease(NERD),the main gastroesophageal reflux subtype,features reflux symptoms without mucosal damage.Anxiety links to visceral hypersensitivity in NERD,yet mechanisms and animal models are unclear.AIM To establish a translational NERD rat model with anxiety comorbidity via tail clamping and study corticotropin-releasing hormone(CRH)-mediated neuroimmune pathways in visceral hypersensitivity and esophageal injury.METHODS Sprague-Dawley(SD)and Wistar rats were grouped into sham,model,and modified groups(n=10 each).The treatments for the modified groups were as follows:SD rats received ovalbumin/aluminum hydroxide suspension+acid perfusion±tail clamping(40 minutes/day for 7 days),while Wistar rats received fructose water+tail clamping.Esophageal pathology,visceral sensitivity,and behavior were assessed.Serum CRH,calcitonin gene-related peptide(CGRP),5-hydroxytryptamine(5-HT),and mast cell tryptase(MCT)and central amygdala(CeA)CRH mRNA were measured via ELISA and qRT-PCR.RESULTS Tail clamping induced anxiety,worsening visceral hypersensitivity(lower abdominal withdrawal reflex thresholds,P<0.05)and esophageal injury(dilated intercellular spaces and mitochondrial edema).Both models showed raised serum CRH,CGRP,5-HT,and MCT(P<0.01)and CeA CRH mRNA expression(P<0.01).Behavioral tests confirmed anxiety-like phenotypes.NERD-anxiety rats showed clinical-like symptom severity without erosion.CONCLUSION Tail clamping induces anxiety in NERD models,worsening visceral hypersensitivity via CRH neuroimmune dysregulation,offering a translational model and highlighting CRH as a treatment target.
基金supported by the Natural Science Foundation of Chongqing,No.CSTB2023NSCQ-mSX0561(to WL).
文摘Effective treatment methods for stroke,a common cerebrovascular disease with a high mortality rate,are still being sought.Exosome therapy,a form of acellular therapy,has demonstrated promising efficacy in various diseases in animal models;however,there is currently insufficient evidence to guide the clinical application of exosome in patients with stroke.This article reviews the progress of exosome applications in stroke treatment.It aims to elucidate the significant potential value of exosomes in stroke therapy and provide a reference for their clinical translation.At present,many studies on exosome-based therapies for stroke are actively underway.Regarding preclinical research,exosomes,as bioactive substances with diverse sources,currently favor stem cells as their origin.Due to their high plasticity,exosomes can be effectively modified through various physical,chemical,and genetic engineering methods to enhance their efficacy.In animal models of stroke,exosome therapy can reduce neuroinflammatory responses,alleviate oxidative stress damage,and inhibit programmed cell death.Additionally,exosomes can promote angiogenesis,repair and regenerate damaged white matter fiber bundles,and facilitate the migration and differentiation of neural stem cells,aiding the repair process.We also summarize new directions for the application of exosomes,specifically the exosome intervention through the ventricular-meningeal lymphatic system.The review findings suggest that the treatment paradigm for stroke is poised for transformation.
基金supported by the Fundamental Research Funds for the Central Universities,Nos.G2021KY05107,G2021KY05101the National Natural Science Foundation of China,Nos.32071316,32211530049+1 种基金the Natural Science Foundation of Shaanxi Province,No.2022-JM482the Education and Teaching Reform Funds for the Central Universities,No.23GZ230102(all to LL and HH).
文摘Although previous studies have demonstrated that transcranial focused ultrasound stimulation protects the ischemic brain,clear criteria for the stimulation time window and intensity are lacking.Electrical impedance tomography enables real-time monitoring of changes in cerebral blood perfusion within the ischemic brain,but investigating the feasibility of using this method to assess post-stroke rehabilitation in vivo remains critical.In this study,ischemic stroke was induced in rats through middle cerebral artery occlusion surgery.Transcranial focused ultrasound stimulation was used to treat the rat model of ischemia,and electrical impedance tomography was used to measure impedance during both the acute stage of ischemia and the rehabilitation stage following the stimulation.Electrical impedance tomography results indicated that cerebral impedance increased after the onset of ischemia and decreased following transcranial focused ultrasound stimulation.Furthermore,the stimulation promoted motor function recovery,reduced cerebral infarction volume in the rat model of ischemic stroke,and induced the expression of brain-derived neurotrophic factor in the ischemic brain.Our results also revealed a significant correlation between the impedance of the ischemic brain post-intervention and improvements in behavioral scores and infarct volume.This study shows that daily administration of transcranial focused ultrasound stimulation for 20 minutes to the ischemic hemisphere 24 hours after cerebral ischemia enhanced motor recovery in a rat model of ischemia.Additionally,our findings indicate that electrical impedance tomography can serve as a valuable tool for quantitatively evaluating rehabilitation after ischemic stroke in vivo.These findings suggest the feasibility of using impedance data collected via electrical impedance tomography to clinically assess the effects of rehabilitatory interventions for patients with ischemic stroke.
文摘Myasthenia gravis is a chronic autoimmune disorder that affects the neuromuscular junction leading to fluctuating skeletal muscle fatigability. The majority of myasthenia gravis patients have detectable antibodies in their serum, targeting acetylcholine receptor, muscle-specific kinase, or related proteins. Current treatment for myasthenia gravis involves symptomatic therapy, immunosuppressive drugs such as corticosteroids, azathioprine, and mycophenolate mofetil, and thymectomy, which is primarily indicated in patients with thymoma or thymic hyperplasia. However, this condition continues to pose significant challenges including an unpredictable and variable disease progression, differing response to individual therapies, and substantial longterm side effects associated with standard treatments(including an increased risk of infections, osteoporosis, and diabetes), underscoring the necessity for a more personalized approach to treatment. Furthermore, about fifteen percent of patients, called “refractory myasthenia gravis patients”, do not respond adequately to standard therapies. In this context, the introduction of molecular therapies has marked a significant advance in myasthenia gravis management. Advances in understanding myasthenia gravis pathogenesis, especially the role of pathogenic antibodies, have driven the development of these biological drugs, which offer more selective, rapid, and safer alternatives to traditional immunosuppressants. This review aims to provide a comprehensive overview of emerging therapeutic strategies targeting specific immune pathways in myasthenia gravis, with a particular focus on preclinical evidence, therapeutic rationale, and clinical translation of B-cell depletion therapies, neonatal Fc receptor inhibitors, and complement inhibitors.
文摘Purpose The paper shows the interpretive impact of different constructions of the point of view available to the reader/viewer in book and animated movie versions of a children's picture book,a novel for pre-adolescents/early teenagers,and a graphic novel for adolescents and adults.Design/Approach/Methods Excerpts from book and animated movie versions of the same story are compared using multimodal analysis of interpersonal meaning to show how the reader/viewer is positioned in relation to the characters in each version,complemented by analyses of ideational meaning to show the effect of point of view on interpretive possibilities.Findings Focusing mainly on multimodal construction of point of view,the analyses show how interpretive possibilities of ostensibly the same story are significantly reconfigured in animated adaptations compared with book versions even when the verbal narrative remains substantially unchanged.Originality/Value The study shows that it is crucial to students’critical appreciation of,and their creative contribution to,their evolving digital literary culture that in this new era of educational technology,attention in literacy and literary education focuses on developing understandings of digital multimodal narrative art,and that animated movie adaptations are not presented pedagogically as isomorphic with,or simply adjunct to,corresponding book versions.
基金supported by the National Natural Science Foundation of China(Grant No.61972379).
文摘Data-driven garment animation is a current topic of interest in the computer graphics industry.Existing approaches generally establish the mapping between a single human pose or a temporal pose sequence,and garment deformation,but it is difficult to quickly generate diverse clothed human animations.We address this problem with a method to automatically synthesize dressed human animations with temporal consistency from a specified human motion label.At the heart of our method is a twostage strategy.Specifically,we first learn a latent space encoding the sequence-level distribution of human motions utilizing a transformer-based conditional variational autoencoder(Transformer-CVAE).Then a garment simulator synthesizes dynamic garment shapes using a transformer encoder-decoder architecture.Since the learned latent space comes from varied human motions,our method can generate a variety of styles of motions given a specific motion label.By means of a novel beginning of sequence(BOS)learning strategy and a self-supervised refinement procedure,our garment simulator is capable of efficiently synthesizing garment deformation sequences corresponding to the generated human motions while maintaining temporal and spatial consistency.We verify our ideas experimentally.This is the first generative model that directly dresses human animation.
基金supported by the National Natural Science Foundation of China(No.81701347,31961133026,81570171,31871187,and 81070455)the National Key R&D Program of China(No.2019YFA0802400)the Priority Academic Program Development(PAPD)of Jiangsu Higher Education Institutions。
文摘Major depressive disorder(MDD)affects people all over the world,and yet,its etiology is complex and remains incompletely understood.In this review,we aim to assess recent advances in understanding depression and its regulation,as well as its interaction with circadian rhythms.Circadian rhythms are internalized representations of the periodic daily light and dark cycles.Accumulating evidence has shown that MDD and the related mental disorders are associated with disrupted circadian rhythms.In particular,depression has often been linked to abnormalities in circadian rhythms because dysregulation of the circadian system increases susceptibility to MDD.The fact that several rhythms are disrupted in depressed patients suggests that these disruptions are not restricted to any one rhythm but rather involve the molecular circadian clock core machinery.The sleep-wake cycle is one rhythm that is often disrupted in depression,which often leads to disturbances in other rhythms.The circadian disruptions manifested in depressed patients and the effectiveness and fast action of chronobiologically based treatments highlight the circadian system as a key therapeutic target in the treatment of depression.This review assesses the evidence on rising depression rates and examines their contributing factors,including circadian misalignment.We discuss key hypotheses underlying depression pathogenesis,potential etiology,and relevant animal models,and underscore potential mechanisms driving depression's growing burden and how understanding these factors is critical for improving prevention and treatment strategies.
基金supported by the Brain&Behavior Research Foundation(30233).
文摘Depressive disorder is a chronic,recurring,and potentially life-endangering neuropsychiatric disease.According to a report by the World Health Organization,the global population suffering from depression is experiencing a significant annual increase.Despite its prevalence and considerable impact on people,little is known about its pathogenesis.One major reason is the scarcity of reliable animal models due to the absence of consensus on the pathology and etiology of depression.Furthermore,the neural circuit mechanism of depression induced by various factors is particularly complex.Considering the variability in depressive behavior patterns and neurobiological mechanisms among different animal models of depression,a comparison between the neural circuits of depression induced by various factors is essential for its treatment.In this review,we mainly summarize the most widely used behavioral animal models and neural circuits under different triggers of depression,aiming to provide a theoretical basis for depression prevention.
基金supported by the National Key Laboratory of Agricultural Microbiology(AML2023A02)the Fundamental Research Funds for the Central Universities(2662023DKPY004).
文摘Rotavirus infections cause severe gastroenteritis and dehydration in young children and animals worldwide,leading to high rates of morbidity and mortality,predominantly in low-and middleincome countries.In the past decade,substantial progress has been made in the development and implementation of rotavirus vaccines,which have been essential in alleviating the global burden of this disease,not only in human being but also in livestock species like calves and piglets,where these infections can cause significant economic losses.By synthesizing the latest research and real-world evidence,this review article is designated to provide deep insights into the current state of rotavirus vaccine technology and its global implementation as well as the application of rotavirus vaccines in veterinary settings and their importance in controlling zoonotic transmission and maintaining food security.
