A new serum-free culture (SFC) system for human AML-CFU was established and the colony-promoting activity of four recombinant human hematopoietic growth factors (rhHGFs) including granulocyte-macrophage colony-stimula...A new serum-free culture (SFC) system for human AML-CFU was established and the colony-promoting activity of four recombinant human hematopoietic growth factors (rhHGFs) including granulocyte-macrophage colony-stimulating factor (rhGM-CSF), interleukin-3 (rhIL-3 ),erythropoietin (rhEPO) and newly developed stem cell factor (rhSCF) were investigated in this .SFC system. Under the orthogonal design condition, it was found that human AML-CFU presented optimal clonal growth in an environment of bovine serum albumin (0. 6 %), saturated human transferrin (2×10-5mol/L),cholesterol (2.8 μg/ml) , bovine insulin (15 μg/ml ) . bovine hemin (0. 05 mmol/L), linoleic acid (2.8 μg/ml) , and IMDM. Spontaneously growing colonies were observed in 11 out of 14 cases studied. The plating efficiencies obtained by culturing with rhGM-CSF, rhIL-3, and rhSCF were 0. 776±0. 621 %, 0. 574±0. 510%, and 0. 647±0. 543 %(x±s), respectively. There was one case (M3b) showing no response to all HGFs in both SFC ad SCC. The clonal growth of AML-CFU obtained from peripheral blood of the patient with M6 was unexpectedly marked. As a whole,the newly designed SFC system has been demonstrated to be useful for culture of human AML-CFU from both bone marrow and peripheral blood.展开更多
文摘A new serum-free culture (SFC) system for human AML-CFU was established and the colony-promoting activity of four recombinant human hematopoietic growth factors (rhHGFs) including granulocyte-macrophage colony-stimulating factor (rhGM-CSF), interleukin-3 (rhIL-3 ),erythropoietin (rhEPO) and newly developed stem cell factor (rhSCF) were investigated in this .SFC system. Under the orthogonal design condition, it was found that human AML-CFU presented optimal clonal growth in an environment of bovine serum albumin (0. 6 %), saturated human transferrin (2×10-5mol/L),cholesterol (2.8 μg/ml) , bovine insulin (15 μg/ml ) . bovine hemin (0. 05 mmol/L), linoleic acid (2.8 μg/ml) , and IMDM. Spontaneously growing colonies were observed in 11 out of 14 cases studied. The plating efficiencies obtained by culturing with rhGM-CSF, rhIL-3, and rhSCF were 0. 776±0. 621 %, 0. 574±0. 510%, and 0. 647±0. 543 %(x±s), respectively. There was one case (M3b) showing no response to all HGFs in both SFC ad SCC. The clonal growth of AML-CFU obtained from peripheral blood of the patient with M6 was unexpectedly marked. As a whole,the newly designed SFC system has been demonstrated to be useful for culture of human AML-CFU from both bone marrow and peripheral blood.
