The use of cooperative vibronic spectroscopy to measure coordination numbers of the ligands surrounding rare earth ions in solutions was proposed and demonstrated. It is shown that the time-resolved cooperative vibron...The use of cooperative vibronic spectroscopy to measure coordination numbers of the ligands surrounding rare earth ions in solutions was proposed and demonstrated. It is shown that the time-resolved cooperative vibronic spectroscopy is a very useful technique for the determination of the ligand type and ligand number for different coordination groups. Coordination configuration of arginine with Tb3+ in solutions with different pH values was studied. It demonstrates that the carboxy1 of arginine can replace H2O to coordinate with Tb3+ and the coordination number of carboxyl increases with the increase of pH value of the solutions.展开更多
D-Aminoacid oxidase (DAO) was isolated from fresh porcine kidney;its cytotoxic potential was studied under in vitro and in vivo conditions. The isolated DAO was complexed with Fe2O3 nanoparticles and its potential as ...D-Aminoacid oxidase (DAO) was isolated from fresh porcine kidney;its cytotoxic potential was studied under in vitro and in vivo conditions. The isolated DAO was complexed with Fe2O3 nanoparticles and its potential as an oxidation therapeutic agent was analysed. The ability of the complex in eliciting H2O2 mediated cytotoxicity was studied on Dalton’s lymphoma ascites cells (DLA). The induction of apoptosis in DLA cells by Fe2O3-DAO complex was studied by morphological examination and alkaline single cell gel electrophoresis (comet assay). The antitumor activity of the complex was investigated by oral administration of the complex and the substrate D-alanine to tumor bearing Swiss albino mice and by targeting the complex to the tumor site, using an externally applied magnetic field. Fe2O3-DAO along with D-alanine showed remarkable cytotoxicity in a substrate concentration-dependent manner. Both morphological examination and comet assay revealed that Fe2O3-DAO/D-alanine induced apoptosis. Oral administration of Fe2O3-DAO and D-alanine along with magnetic targeting significantly suppressed tumor growth in mice. The present report provides the first evidence for the promising application of enzyme bound nanoparticles for targeted oxidation therapy.展开更多
Biological sensing technology plays a crucial role in various key areas such as disease diagnosis,environmental monitoring,and biotechnology.Luminescent metal-organic frameworks(LMOFs),with their remarkable advantages...Biological sensing technology plays a crucial role in various key areas such as disease diagnosis,environmental monitoring,and biotechnology.Luminescent metal-organic frameworks(LMOFs),with their remarkable advantages including large surface area,customizable pore structures,and highly active functional sites,have emerged as a frontier in biosensor research.This review clarifies the potential of LMOFs in biological sensing applications,with particular emphasis on their efficient performance in detecting amino acids,biomarkers,and drugs,explore the possibility of integrating LMOFs with portable analytical techniques,providing an innovative perspective for advancing luminescence detection technology.Some effective characterization methods to encode these sensing mechanisms including F?rster resonance energy transfer(FRET),photoinduced electron transfer(PET)and thermally activated energy back transfer(BENT)highlighted their connection and difference.Finally,the article summarizes the achievements of LMOFs in biological sensing and provides a perspective on future research directions and potential applications,aiming to propel the continuous development of this field.展开更多
Nine C2-symmetric chiral disulfonamldes were designed and synthesized from L-valine, L-phenylalanine and L-leucine respectively via one-pot ring opening of aziridines. The application of these disulfonamides as cataly...Nine C2-symmetric chiral disulfonamldes were designed and synthesized from L-valine, L-phenylalanine and L-leucine respectively via one-pot ring opening of aziridines. The application of these disulfonamides as catalyst in the addition of diethyl zinc to benzaldehyde in three reagent systems: Ligand-Ti(OiPr)4-Et2Zn; Ligand-Ti(OiPr)4-H2O-Et2Zn; Ligand-Et2Zn was also described.展开更多
基金the Natural Science Foundation of Guangdong Province!980278, 960036the National Natural Science Foundation of China!29705004
文摘The use of cooperative vibronic spectroscopy to measure coordination numbers of the ligands surrounding rare earth ions in solutions was proposed and demonstrated. It is shown that the time-resolved cooperative vibronic spectroscopy is a very useful technique for the determination of the ligand type and ligand number for different coordination groups. Coordination configuration of arginine with Tb3+ in solutions with different pH values was studied. It demonstrates that the carboxy1 of arginine can replace H2O to coordinate with Tb3+ and the coordination number of carboxyl increases with the increase of pH value of the solutions.
文摘D-Aminoacid oxidase (DAO) was isolated from fresh porcine kidney;its cytotoxic potential was studied under in vitro and in vivo conditions. The isolated DAO was complexed with Fe2O3 nanoparticles and its potential as an oxidation therapeutic agent was analysed. The ability of the complex in eliciting H2O2 mediated cytotoxicity was studied on Dalton’s lymphoma ascites cells (DLA). The induction of apoptosis in DLA cells by Fe2O3-DAO complex was studied by morphological examination and alkaline single cell gel electrophoresis (comet assay). The antitumor activity of the complex was investigated by oral administration of the complex and the substrate D-alanine to tumor bearing Swiss albino mice and by targeting the complex to the tumor site, using an externally applied magnetic field. Fe2O3-DAO along with D-alanine showed remarkable cytotoxicity in a substrate concentration-dependent manner. Both morphological examination and comet assay revealed that Fe2O3-DAO/D-alanine induced apoptosis. Oral administration of Fe2O3-DAO and D-alanine along with magnetic targeting significantly suppressed tumor growth in mice. The present report provides the first evidence for the promising application of enzyme bound nanoparticles for targeted oxidation therapy.
基金supported financially by Natural Science Foundation of Tianjin(No.18JCYBJC89700)Science&Technology Development Fund of Tianjin Education Commission for Higher Education(No.2019ZD15)。
文摘Biological sensing technology plays a crucial role in various key areas such as disease diagnosis,environmental monitoring,and biotechnology.Luminescent metal-organic frameworks(LMOFs),with their remarkable advantages including large surface area,customizable pore structures,and highly active functional sites,have emerged as a frontier in biosensor research.This review clarifies the potential of LMOFs in biological sensing applications,with particular emphasis on their efficient performance in detecting amino acids,biomarkers,and drugs,explore the possibility of integrating LMOFs with portable analytical techniques,providing an innovative perspective for advancing luminescence detection technology.Some effective characterization methods to encode these sensing mechanisms including F?rster resonance energy transfer(FRET),photoinduced electron transfer(PET)and thermally activated energy back transfer(BENT)highlighted their connection and difference.Finally,the article summarizes the achievements of LMOFs in biological sensing and provides a perspective on future research directions and potential applications,aiming to propel the continuous development of this field.
文摘Nine C2-symmetric chiral disulfonamldes were designed and synthesized from L-valine, L-phenylalanine and L-leucine respectively via one-pot ring opening of aziridines. The application of these disulfonamides as catalyst in the addition of diethyl zinc to benzaldehyde in three reagent systems: Ligand-Ti(OiPr)4-Et2Zn; Ligand-Ti(OiPr)4-H2O-Et2Zn; Ligand-Et2Zn was also described.
