Herbicide resistance in crop plants is valuable for integrated weed management in agriculture. Herbicide resistant rice, in particular, is important to management of weedy rice, a close relative of cultivated rice and...Herbicide resistance in crop plants is valuable for integrated weed management in agriculture. Herbicide resistant rice, in particular, is important to management of weedy rice, a close relative of cultivated rice and a noxious weed prevalent in rice fields that remains challenging to farmers worldwide. Herbicide resistant plants can be obtained through transgenic approach or by mutagenesis of regular plant and screening of mutants with elevated resistance to herbicide. In this study, we conducted ethyl methyl sulfonate mutagenesis(EMS) to elite indica cultivar Huanghuazhan(HHZ) and screened for mutants resistant to imazapic, a herbicide that can inhibit the acetolactate synthase(ALS) in plants. We obtained three mutants of Os ALS gene that have not been reported previously in rice. One of the mutants, with Trp_(548) changed to Met(W_(548)M), was analyzed in more details in this study. This mutation had no negative effect on the plant physiology and morphology as well as rice yield. Compared with the imidazolinone-resistant mutant S_(627)N(Ser_(627) changed to Asn) that has been deployed for Clearfield rice development, W_(548)M mutant showed high levels of resistance to a broad spectrum of five families of ALSinhibiting herbicides, in addition to a higher level of resistance to herbicides of the imidazolinone family.The herbicide-resistance was stably inherited by crossing into other rice lines. Thus, the W_(548)M mutation provides a valuable resource for breeding of herbicide resistant rice and weed management.展开更多
The resistance and cross-resistance of Scirpus juncoides Roxb.to ALS-inhibiting herbicides were tested to provide theoretical basis for effectively control Scirpus juncoides Roxb.and rational use of herbicides.To iden...The resistance and cross-resistance of Scirpus juncoides Roxb.to ALS-inhibiting herbicides were tested to provide theoretical basis for effectively control Scirpus juncoides Roxb.and rational use of herbicides.To identify the resistance and crossresistance of 19 populations from nine areas in Heilongjiang Province to four ALS-inhibiting herbicides by agar method and the whole-plant assay.The results are as the followings,the two bioassay methods had good correlation,the resistance indexes in the same area were different by two means,but resistant level was the same,similarity up to 89.5%.The resistant rates to pyrazosulfuronethyl,bensulfuron-methyl,penoxsulam and pyribenzoxim were 26.3%,36.8%,36.1%and 21.1%,respectively.Shangzhi populations evolved the highest level of resistance to pyrazosulfuron-methyl with a resistance index of 15.56.Shangzhi,Tonghe,Fangzheng and Jixi populations showed cross-resistance to four ALS-inhibiting herbicides,accounting for 26.3%.The resistance of Scirpus juncoides Roxb.to four ALS-inhibiting herbicides in Heilongjiang paddy field was low to moderate,five populations showed cross-resistance to four ALS-inhibiting herbicides.展开更多
The aim of this study was to compare control of three stages biotypes (rosette, bolting, and repro- ductive stage) of Conyza bonariensis glyphosate susceptible biotype (S) and tolerant biotype (T) to glyphosate with g...The aim of this study was to compare control of three stages biotypes (rosette, bolting, and repro- ductive stage) of Conyza bonariensis glyphosate susceptible biotype (S) and tolerant biotype (T) to glyphosate with glyphosate and mixtures of acetolactate synthase (ALS)-inhibiting. For glyphosate, the dose-response curves confirmed that injury of the biotype T relative to biotype S was signifi- cantly lower for both rosette and bolting stages. Resistance index (RI) for this herbicide was approximately 4 for both weed stages. At bolting, for both biotypes doses much higher than the recommended dose were required. For acetolactate synthase (ALS)-inhibiting herbicides, at the rosette stage, control of both biotypes was excellent with doses significantly lower than the recommended rate. All herbicides within this group showed a very low I50 relative to the recommended rate. These results indicate that biotypes difficult to control with glyphosate at the rosette and bolting stage may be controlled using acetolacte synthase (ALS)-inhibiting herbicides. Control for the reproductive stage was poor. A management program based on the combination of glyphosate with pre- and post-emergence acetolactate synthase (ALS) herbicides may be effective to control the weed.展开更多
基金supported by Major Program of Guangdong Basic and Applied Research(2019B030302006)National Natural Science Foundation of China(U1901203 and 31901532)+2 种基金Natural Science Foundation of Guangdong Province(2018B030308008 and 2018A0303130270)Shenzhen Commission on Innovation and Technology Programs(JCYJ20180507181837997)China Postdoctoral Science Foundation(2018M633069 and 2019M652920)。
文摘Herbicide resistance in crop plants is valuable for integrated weed management in agriculture. Herbicide resistant rice, in particular, is important to management of weedy rice, a close relative of cultivated rice and a noxious weed prevalent in rice fields that remains challenging to farmers worldwide. Herbicide resistant plants can be obtained through transgenic approach or by mutagenesis of regular plant and screening of mutants with elevated resistance to herbicide. In this study, we conducted ethyl methyl sulfonate mutagenesis(EMS) to elite indica cultivar Huanghuazhan(HHZ) and screened for mutants resistant to imazapic, a herbicide that can inhibit the acetolactate synthase(ALS) in plants. We obtained three mutants of Os ALS gene that have not been reported previously in rice. One of the mutants, with Trp_(548) changed to Met(W_(548)M), was analyzed in more details in this study. This mutation had no negative effect on the plant physiology and morphology as well as rice yield. Compared with the imidazolinone-resistant mutant S_(627)N(Ser_(627) changed to Asn) that has been deployed for Clearfield rice development, W_(548)M mutant showed high levels of resistance to a broad spectrum of five families of ALSinhibiting herbicides, in addition to a higher level of resistance to herbicides of the imidazolinone family.The herbicide-resistance was stably inherited by crossing into other rice lines. Thus, the W_(548)M mutation provides a valuable resource for breeding of herbicide resistant rice and weed management.
基金Supported by the National Key Research and Development Program of China(2017YFD0200307)
文摘The resistance and cross-resistance of Scirpus juncoides Roxb.to ALS-inhibiting herbicides were tested to provide theoretical basis for effectively control Scirpus juncoides Roxb.and rational use of herbicides.To identify the resistance and crossresistance of 19 populations from nine areas in Heilongjiang Province to four ALS-inhibiting herbicides by agar method and the whole-plant assay.The results are as the followings,the two bioassay methods had good correlation,the resistance indexes in the same area were different by two means,but resistant level was the same,similarity up to 89.5%.The resistant rates to pyrazosulfuronethyl,bensulfuron-methyl,penoxsulam and pyribenzoxim were 26.3%,36.8%,36.1%and 21.1%,respectively.Shangzhi populations evolved the highest level of resistance to pyrazosulfuron-methyl with a resistance index of 15.56.Shangzhi,Tonghe,Fangzheng and Jixi populations showed cross-resistance to four ALS-inhibiting herbicides,accounting for 26.3%.The resistance of Scirpus juncoides Roxb.to four ALS-inhibiting herbicides in Heilongjiang paddy field was low to moderate,five populations showed cross-resistance to four ALS-inhibiting herbicides.
文摘The aim of this study was to compare control of three stages biotypes (rosette, bolting, and repro- ductive stage) of Conyza bonariensis glyphosate susceptible biotype (S) and tolerant biotype (T) to glyphosate with glyphosate and mixtures of acetolactate synthase (ALS)-inhibiting. For glyphosate, the dose-response curves confirmed that injury of the biotype T relative to biotype S was signifi- cantly lower for both rosette and bolting stages. Resistance index (RI) for this herbicide was approximately 4 for both weed stages. At bolting, for both biotypes doses much higher than the recommended dose were required. For acetolactate synthase (ALS)-inhibiting herbicides, at the rosette stage, control of both biotypes was excellent with doses significantly lower than the recommended rate. All herbicides within this group showed a very low I50 relative to the recommended rate. These results indicate that biotypes difficult to control with glyphosate at the rosette and bolting stage may be controlled using acetolacte synthase (ALS)-inhibiting herbicides. Control for the reproductive stage was poor. A management program based on the combination of glyphosate with pre- and post-emergence acetolactate synthase (ALS) herbicides may be effective to control the weed.
