We isolated a novel strain of Alphaproteobacteria from a patient,who had medical history of chronic rhinitis for more than twenty years and recently experienced local skin abscess and ulcer. He eventually died of mult...We isolated a novel strain of Alphaproteobacteria from a patient,who had medical history of chronic rhinitis for more than twenty years and recently experienced local skin abscess and ulcer. He eventually died of multiple organ failure due to multi-antibiotics resistance.We identified the microorganism by 16SrRNA sequencing and found that it belonged to the genus Rhodoplanes. It was named as Rhodoplanes sp.strain ZLJ-0.It is resumed that Rhodoplanes sp.strain ZLJ-0 might be an emerging human pathogen involving in unknown febrile conditions and could cause local infection of any tissues or organs.Differential diagnosis of febrile patients should be conducted in clinical practice and research on emerging pathogens of Alphaproteobacteria should be performed to determine the epidemiology,clinical symptoms and pathogenic features of these pathogens.展开更多
Aerobic anoxygenic phototrophic bacteria(AAPB)repre sent a major group of bacterioplankton assemblages in many water systems and some are assumed to be closely associated with phytoplankton.However,studies on relation...Aerobic anoxygenic phototrophic bacteria(AAPB)repre sent a major group of bacterioplankton assemblages in many water systems and some are assumed to be closely associated with phytoplankton.However,studies on relationships between AAPB and cyanobacterial blooms are in scarcity.The dynamics of the abundance and diversity of AAPB was compared based on pufM gene in Meiliang Bay(featured by cyanobacterial blooms)and East Bay(featured by macrophyte)of Taihu Lake,a shallow subtropical lake in the East China plain.AAPB abundance was not significantly different between the two sites,and they were positively correlated with dissolved organic carbon(DOC)concentration.The ratios of AAPB to total bacteria varied from 3.4%to 11.5%and peaked in winter in both site s.No significant differences of AAPB community compositions were detected between the two sites,but there was a separation between warm seasons(June,August,and October)and cold seasons(December,February,and April).Rhizobiales and Limnohabitans-like pufM sequences were significantly contributors for the difference between two seasons,and specially enriched in cold seasons.Chlorophyll a(Ch1 a)and DOC were the most significant variables influencing the AAPB community structure.Furthermore,Porphyrobacter and Rhodospirillales-like pufM sequences were positively correlated with Ch1 a,indicating potential influence of cyanobacterial blooms on these AAPB taxa.These results suggested that diverse AAPB ecotypes coexisted in Taihu Lake,and their ecological role in carbon cycling in the lake may not be ignored.展开更多
Magnetotactic bacteria are a group of Gram-negative bacteria that synthesize magnetic crystals, enabling them to navigate in relation to magnetic field lines. Morphologies of magnetotactic bacteria include spirillum, ...Magnetotactic bacteria are a group of Gram-negative bacteria that synthesize magnetic crystals, enabling them to navigate in relation to magnetic field lines. Morphologies of magnetotactic bacteria include spirillum, coccoid, rod, vibrio, and multicellular morphotypes. The coccid shape is generally the most abundant morphotype among magnetotactic bacteria. Here we describe a species of giant rod-shaped magnetotactic bacteria(designated QR-1) collected from sediment in the low tide zone of Huiquan Bay(Yellow Sea, China). This morphotype accounted for 90% of the magnetotactic bacteria collected, and the only taxonomic group which was detected in the sampling site. Microscopy analysis revealed that QR-1 cells averaged(6.71±1.03)×(1.54±0.20) m m in size, and contained in each cell 42–146 magnetosomes that are arranged in a bundle formed one to four chains along the long axis of the cell. The QR-1 cells displayed axial magnetotaxis with an average velocity of 70±28 mm/s. Transmission electron microscopy based analysis showed that QR-1 cells had two tufts of fl agella at each end. Phylogenetic analysis of the 16 S r RNA genes revealed that QR-1 together with three other rod-shaped uncultivated magnetotactic bacteria are clustered into a deep branch of A lphaproteobacteria.展开更多
The vast diversity of soil bacteria provides essential ecosystem services that support agricultural production.Variation in the diversity and composition of soil biota may have predictive values for soil nutrient cycl...The vast diversity of soil bacteria provides essential ecosystem services that support agricultural production.Variation in the diversity and composition of soil biota may have predictive values for soil nutrient cycling and resilience of ecosystem services,thus providing valuable insights to improve food production.The North China Plain(NCP)is one of the world’s key agricultural regions,supplying more than 50% of the cereal consumed in Asia.However,it is unknown whether soil microbial diversity is predictable across the NCP.Using the MiSeq Illumina platform,we examined bacterial community variation in relation to spatial and environmental factors from 243 soils in wheat-maize double cropping rotation fields across the NCP,which cover nearly 0.3 million km^(2).Based on observed bacterial communities and their relationships with environmental factors,we generated a map of bacterial communities across the NCP.The highest bacterial diversity was found in the middle part of the NCP,with most of the variation in diversity attributable to differences in the community similarity of Actinobacteria and Alphaproteobacteria.These findings provide important baseline information for analyzing the relationships between microbial community,soil functionality and crop yields.展开更多
基金funded by the National Natural Science Foundation of China(No.30771854)National Basic Research Program of China(973 Program) 2010CB530200 (2010CB530206)
文摘We isolated a novel strain of Alphaproteobacteria from a patient,who had medical history of chronic rhinitis for more than twenty years and recently experienced local skin abscess and ulcer. He eventually died of multiple organ failure due to multi-antibiotics resistance.We identified the microorganism by 16SrRNA sequencing and found that it belonged to the genus Rhodoplanes. It was named as Rhodoplanes sp.strain ZLJ-0.It is resumed that Rhodoplanes sp.strain ZLJ-0 might be an emerging human pathogen involving in unknown febrile conditions and could cause local infection of any tissues or organs.Differential diagnosis of febrile patients should be conducted in clinical practice and research on emerging pathogens of Alphaproteobacteria should be performed to determine the epidemiology,clinical symptoms and pathogenic features of these pathogens.
基金Supported by the National Natural Science Foundation of China(Nos.31971449,31370509,31100363)the CAS-SAFEA International Partnership Program for Creative Research Teams(CN)(No.KZZD-EWTZ-08)the startup funds from Nanjing Institute of Geography and Limnology,Chinese Academy of Sciences(No.NIGLAS2011QD05)。
文摘Aerobic anoxygenic phototrophic bacteria(AAPB)repre sent a major group of bacterioplankton assemblages in many water systems and some are assumed to be closely associated with phytoplankton.However,studies on relationships between AAPB and cyanobacterial blooms are in scarcity.The dynamics of the abundance and diversity of AAPB was compared based on pufM gene in Meiliang Bay(featured by cyanobacterial blooms)and East Bay(featured by macrophyte)of Taihu Lake,a shallow subtropical lake in the East China plain.AAPB abundance was not significantly different between the two sites,and they were positively correlated with dissolved organic carbon(DOC)concentration.The ratios of AAPB to total bacteria varied from 3.4%to 11.5%and peaked in winter in both site s.No significant differences of AAPB community compositions were detected between the two sites,but there was a separation between warm seasons(June,August,and October)and cold seasons(December,February,and April).Rhizobiales and Limnohabitans-like pufM sequences were significantly contributors for the difference between two seasons,and specially enriched in cold seasons.Chlorophyll a(Ch1 a)and DOC were the most significant variables influencing the AAPB community structure.Furthermore,Porphyrobacter and Rhodospirillales-like pufM sequences were positively correlated with Ch1 a,indicating potential influence of cyanobacterial blooms on these AAPB taxa.These results suggested that diverse AAPB ecotypes coexisted in Taihu Lake,and their ecological role in carbon cycling in the lake may not be ignored.
基金Supported by the National Natural Science Foundation of China(Nos.41330962,41276170)the National Natural Science Foundation of China-Shandong Joint Fund for Marine Science Research Centers(No.U1606404)
文摘Magnetotactic bacteria are a group of Gram-negative bacteria that synthesize magnetic crystals, enabling them to navigate in relation to magnetic field lines. Morphologies of magnetotactic bacteria include spirillum, coccoid, rod, vibrio, and multicellular morphotypes. The coccid shape is generally the most abundant morphotype among magnetotactic bacteria. Here we describe a species of giant rod-shaped magnetotactic bacteria(designated QR-1) collected from sediment in the low tide zone of Huiquan Bay(Yellow Sea, China). This morphotype accounted for 90% of the magnetotactic bacteria collected, and the only taxonomic group which was detected in the sampling site. Microscopy analysis revealed that QR-1 cells averaged(6.71±1.03)×(1.54±0.20) m m in size, and contained in each cell 42–146 magnetosomes that are arranged in a bundle formed one to four chains along the long axis of the cell. The QR-1 cells displayed axial magnetotaxis with an average velocity of 70±28 mm/s. Transmission electron microscopy based analysis showed that QR-1 cells had two tufts of fl agella at each end. Phylogenetic analysis of the 16 S r RNA genes revealed that QR-1 together with three other rod-shaped uncultivated magnetotactic bacteria are clustered into a deep branch of A lphaproteobacteria.
基金supported by the Strategic Priority Research Program(XDB15010101)of the Chinese Academy of Sciencesthe National Key Research and Development Program of China(2017YFD0200604,2017YFC0803803)+2 种基金the“135”Plan and Frontiers Projects of Institute of Soil Science(ISSASIP1641)The collaborative innovation project from the Forensic Appraisal Center of The ministry of Public Security of the People’s Republic of China(2016XTCX02)the China Biodiversity Observation Networks(Sino BON).
文摘The vast diversity of soil bacteria provides essential ecosystem services that support agricultural production.Variation in the diversity and composition of soil biota may have predictive values for soil nutrient cycling and resilience of ecosystem services,thus providing valuable insights to improve food production.The North China Plain(NCP)is one of the world’s key agricultural regions,supplying more than 50% of the cereal consumed in Asia.However,it is unknown whether soil microbial diversity is predictable across the NCP.Using the MiSeq Illumina platform,we examined bacterial community variation in relation to spatial and environmental factors from 243 soils in wheat-maize double cropping rotation fields across the NCP,which cover nearly 0.3 million km^(2).Based on observed bacterial communities and their relationships with environmental factors,we generated a map of bacterial communities across the NCP.The highest bacterial diversity was found in the middle part of the NCP,with most of the variation in diversity attributable to differences in the community similarity of Actinobacteria and Alphaproteobacteria.These findings provide important baseline information for analyzing the relationships between microbial community,soil functionality and crop yields.
