Hypoxia-Ischemia Brain Damage(HIBD)results in a widespread neuronal damage and permanent brain tissue injury due to the severe reactive oxygen species(ROS)boost induced neuroinflammation.In this work,a K doped N-C bas...Hypoxia-Ischemia Brain Damage(HIBD)results in a widespread neuronal damage and permanent brain tissue injury due to the severe reactive oxygen species(ROS)boost induced neuroinflammation.In this work,a K doped N-C based nanozyme was fabricated for scavenging ROS.KNC could impair ROS production and M1 polarization in microglia.Beneficial from these contents,the brain damage was mitigated in HIBD rats,which was proved by the increased regional blood flow,decreased pro-inflammatory microglia and astrocyte activation.The learning and memory capabilities were restored after applying with KNC post HIBD,which was ascribed to the diminished HI-induced dendritic spine loss in hippocampal regions.RNA-seq revealed that decreased ALOX12 expression is one of the clues of neuronal protection.KNC could combine with ALOX12 and further inhibit the lipid peroxi-dation.These two clues obtain KNC with superior ability of inhibition of ROS boost induced brain damage post HIBD.This nanozyme provided a potential strategies and new idea of HIBD therapy.展开更多
基金Financial support from the Science and Technology Commission of Shanghai Municipality(24CL2900700)the Fundamental Research Funds for The Central Universities(YG2023ZD29)+5 种基金the National Natural Science Foundation of China(32371397,U21A20100)the Funda-mental Research Funds for The Central Universities(2232023A-10)the Research Fund of Shanghai Tongren Hospital,Shanghai Jiaotong Uni-versity School of Medicine(2023DHYGJC-YBA01)the Laboratory Open Fund of Key Technology and Materials in Minimally Invasive Spine Surgery(2024JZWC-ZDB03)Natural Science Foundation of Shanghai(24ZR1463600)the Talent project of Shanghai Tongren Hospital(TRKYRC-xx02)are acknowledged.
文摘Hypoxia-Ischemia Brain Damage(HIBD)results in a widespread neuronal damage and permanent brain tissue injury due to the severe reactive oxygen species(ROS)boost induced neuroinflammation.In this work,a K doped N-C based nanozyme was fabricated for scavenging ROS.KNC could impair ROS production and M1 polarization in microglia.Beneficial from these contents,the brain damage was mitigated in HIBD rats,which was proved by the increased regional blood flow,decreased pro-inflammatory microglia and astrocyte activation.The learning and memory capabilities were restored after applying with KNC post HIBD,which was ascribed to the diminished HI-induced dendritic spine loss in hippocampal regions.RNA-seq revealed that decreased ALOX12 expression is one of the clues of neuronal protection.KNC could combine with ALOX12 and further inhibit the lipid peroxi-dation.These two clues obtain KNC with superior ability of inhibition of ROS boost induced brain damage post HIBD.This nanozyme provided a potential strategies and new idea of HIBD therapy.
