Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA （RAPD） marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus ou...Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA （RAPD） marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus outcrossing rate estimated based on all 25 RAPD loci was 0.872 ±0.033 and the single-locus outcrossing rate was 0.795 ±0.032. Multilocus esti- mates did not differ significantly from the single-locus estimates. The fixation index, F, in the progeny estimated from RAPD data was -0.142 ±0.000. The estimates of multilocus outcrossing rates （tm） and single-locus outcrossing rates （ts） obtained from MLDT clearly indicate that outcrossing is predominant in the open-pollinated C. grandis population. An empirical analysis suggests that 15 should be the minimum number of dominant marker loci necessary to achieve robust estimates of tm.展开更多
Andromonoecy is a rare sexual system in plants.The function of additional male flowers in andromonoecious species has been widely discussed;however,few studies have taken offspring fitness into account.In addition,lit...Andromonoecy is a rare sexual system in plants.The function of additional male flowers in andromonoecious species has been widely discussed;however,few studies have taken offspring fitness into account.In addition,little is known about the mechanisms that maintain andromonoecy in autogamous species.In this study,we compared morphology,pollinator preference,pollen production and export,siring ability,natural siring success,hundred seed dry weight,and seed germination rates between male and hermaphroditic flowers in an endangered autogamous andromonoecious species,Sagittaria guayanensis.Male flowers,which are larger than hermaphroditic flowers,required fewer resources to produce.Pollinators visited male flowers more frequently than they visited hermaphroditic flowers.In addition,pollen production and export were higher in male flowers.Hand pollination demonstrated that siring ability did not differ between flower type.However,the natural siring success of male flowers was triple that of hermaphroditic flowers.The seeds sired by male flowers performed better than those sired by hermaphroditic flowers,with greater dry weight and higher germination rate.In conclusion,male flowers may be superior pollen donors for outcrossing.The maintenance of andromonoecy in S.guayanensis may result from the better performance of male flowers in male function compared to that of hermaphroditic flowers.展开更多
Anthropogenic introduction of species has resulted in a breakdown of geographical barriers and hybridization in previously allopatric species.Thus,examining hybridization proneness of exotic species contributes to rev...Anthropogenic introduction of species has resulted in a breakdown of geographical barriers and hybridization in previously allopatric species.Thus,examining hybridization proneness of exotic species contributes to revealing its potential threat.Moreover,reproductive barriers may be strengthened or weakened due to long-term geographical isolation for these newly sympatric species.However,few studies have evaluated multiple barriers between alien and native species.In this study,we quantified the importance of four pre-pollination barriers(phenological,floral traits,pollen production,and floral constancy)and four post-pollination barriers(pollen-pistil incompatibility,seed set,seed viability,and seedling survival)between two introduced and five native Sagittaria species.Results showed that introduced S.platyphylla was cross-compatible with two native species,whereas introduced S.montevidensis was incapable of hybridizing with any native species.Different barriers were asymmetric within species pairs and multiple barriers acted in concert to maintain species boundaries.Postpollination barriers contributed more to total reproductive isolation in native species,whereas prepollination barriers played a stronger role in total reproductive isolation for two introduced species.Seed set was the only barrier that was positively correlated with genetic distance.Our results provide a perspective to better understand reproductive barriers for secondary contact species.We highlight the importance of monitoring hybridization events before human introduction and the possible conservation strategies to remove invasive species with hybridization proneness.展开更多
基金Supported by the State Key Basic Research and Develop-ment Plan (G2000046805)the National Natural Science Foundation of China (30370098, 30570111)
文摘Outcrossing rate in a natural population of Caldesia grandis was estimated by the dominant random amplified polymorphism DNA （RAPD） marker using 10 open-pollinated progeny arrays of 24 individuals. The multilocus outcrossing rate estimated based on all 25 RAPD loci was 0.872 ±0.033 and the single-locus outcrossing rate was 0.795 ±0.032. Multilocus esti- mates did not differ significantly from the single-locus estimates. The fixation index, F, in the progeny estimated from RAPD data was -0.142 ±0.000. The estimates of multilocus outcrossing rates （tm） and single-locus outcrossing rates （ts） obtained from MLDT clearly indicate that outcrossing is predominant in the open-pollinated C. grandis population. An empirical analysis suggests that 15 should be the minimum number of dominant marker loci necessary to achieve robust estimates of tm.
基金supported by the National Natural Science Foundation of China(No.31970250).
文摘Andromonoecy is a rare sexual system in plants.The function of additional male flowers in andromonoecious species has been widely discussed;however,few studies have taken offspring fitness into account.In addition,little is known about the mechanisms that maintain andromonoecy in autogamous species.In this study,we compared morphology,pollinator preference,pollen production and export,siring ability,natural siring success,hundred seed dry weight,and seed germination rates between male and hermaphroditic flowers in an endangered autogamous andromonoecious species,Sagittaria guayanensis.Male flowers,which are larger than hermaphroditic flowers,required fewer resources to produce.Pollinators visited male flowers more frequently than they visited hermaphroditic flowers.In addition,pollen production and export were higher in male flowers.Hand pollination demonstrated that siring ability did not differ between flower type.However,the natural siring success of male flowers was triple that of hermaphroditic flowers.The seeds sired by male flowers performed better than those sired by hermaphroditic flowers,with greater dry weight and higher germination rate.In conclusion,male flowers may be superior pollen donors for outcrossing.The maintenance of andromonoecy in S.guayanensis may result from the better performance of male flowers in male function compared to that of hermaphroditic flowers.
基金This study was supported by the National Science Foundation of China(31970250).
文摘Anthropogenic introduction of species has resulted in a breakdown of geographical barriers and hybridization in previously allopatric species.Thus,examining hybridization proneness of exotic species contributes to revealing its potential threat.Moreover,reproductive barriers may be strengthened or weakened due to long-term geographical isolation for these newly sympatric species.However,few studies have evaluated multiple barriers between alien and native species.In this study,we quantified the importance of four pre-pollination barriers(phenological,floral traits,pollen production,and floral constancy)and four post-pollination barriers(pollen-pistil incompatibility,seed set,seed viability,and seedling survival)between two introduced and five native Sagittaria species.Results showed that introduced S.platyphylla was cross-compatible with two native species,whereas introduced S.montevidensis was incapable of hybridizing with any native species.Different barriers were asymmetric within species pairs and multiple barriers acted in concert to maintain species boundaries.Postpollination barriers contributed more to total reproductive isolation in native species,whereas prepollination barriers played a stronger role in total reproductive isolation for two introduced species.Seed set was the only barrier that was positively correlated with genetic distance.Our results provide a perspective to better understand reproductive barriers for secondary contact species.We highlight the importance of monitoring hybridization events before human introduction and the possible conservation strategies to remove invasive species with hybridization proneness.
