Asparagine-linked glycosylation protein 1 homolog(ALG1)participates in the initial stage of protein N-glycosylation and N-glycosylation has been implicated in the process of hepatocellular carcinoma(HCC)progression.Ho...Asparagine-linked glycosylation protein 1 homolog(ALG1)participates in the initial stage of protein N-glycosylation and N-glycosylation has been implicated in the process of hepatocellular carcinoma(HCC)progression.However,whether ALG1 plays a role in human HCC remains unknown.In this study,the expression profile of ALG1 in tumorous and corresponding adjacent non-tumor tissues was analyzed.The relationship of ALG1 expression with clinical features and prognosis of HCC patients was also evaluated using immuno-histochemical method.Here we found ALG1 decreased in HCC tissues compared with adjacent normal liver tissues,which predicted an unfavorable prognosis.Combined with RNA interference,nascent proteome and glycoproteome were determined systematically in Huh7 cell line.Bioinformatics analysis indicated that the differentially expressed proteins participating in the response of ALG1 knockdown were most significantly associated with cell-cell adhesion.Functional studies confirmed that knockdown of ALG1 reduced cell adhesion capacity,and promoted cell migration.Furthermore,down-regulation of H8N2(on N-glycosite N651)and H5N4S2F1(on N-glycosite N692)from N-cadherin was identified as a feature of ALG1 knockdown.Our findings revealed that ALG1 controlled the expression of glycosylated N-cadherin and played a role in HCC migration,with implications for prognosis.展开更多
本文采用缓慢冷却方法制备了磁性拓扑材料Mn_(1-x)Fe_(x)Al Ge系列单晶样品,并系统研究了Fe掺杂对体系的结构、磁性以及反常霍尔效应的影响.随着Fe含量的增加,体系的晶胞收缩,磁性逐渐降低.在Mn Al Ge中观测到高达~800Ω^(-1)cm^(-1)的...本文采用缓慢冷却方法制备了磁性拓扑材料Mn_(1-x)Fe_(x)Al Ge系列单晶样品,并系统研究了Fe掺杂对体系的结构、磁性以及反常霍尔效应的影响.随着Fe含量的增加,体系的晶胞收缩,磁性逐渐降低.在Mn Al Ge中观测到高达~800Ω^(-1)cm^(-1)的反常霍尔电导率(AHC),并且随着Fe含量增加,体系的AHC逐渐降低.采用对数和TYJ模型对体系AHC的内外禀贡献进行定性和定量分离,其结果显示所有样品中内外禀机制对总的AHC贡献相当,并且两者均随着Fe含量的增加而降低.展开更多
基金the financial support of the National Key Research and Development Program of China(2017YFA0505100)NSF of China(Grants 21974025 and 82121004)for this work.
文摘Asparagine-linked glycosylation protein 1 homolog(ALG1)participates in the initial stage of protein N-glycosylation and N-glycosylation has been implicated in the process of hepatocellular carcinoma(HCC)progression.However,whether ALG1 plays a role in human HCC remains unknown.In this study,the expression profile of ALG1 in tumorous and corresponding adjacent non-tumor tissues was analyzed.The relationship of ALG1 expression with clinical features and prognosis of HCC patients was also evaluated using immuno-histochemical method.Here we found ALG1 decreased in HCC tissues compared with adjacent normal liver tissues,which predicted an unfavorable prognosis.Combined with RNA interference,nascent proteome and glycoproteome were determined systematically in Huh7 cell line.Bioinformatics analysis indicated that the differentially expressed proteins participating in the response of ALG1 knockdown were most significantly associated with cell-cell adhesion.Functional studies confirmed that knockdown of ALG1 reduced cell adhesion capacity,and promoted cell migration.Furthermore,down-regulation of H8N2(on N-glycosite N651)and H5N4S2F1(on N-glycosite N692)from N-cadherin was identified as a feature of ALG1 knockdown.Our findings revealed that ALG1 controlled the expression of glycosylated N-cadherin and played a role in HCC migration,with implications for prognosis.
文摘本文采用缓慢冷却方法制备了磁性拓扑材料Mn_(1-x)Fe_(x)Al Ge系列单晶样品,并系统研究了Fe掺杂对体系的结构、磁性以及反常霍尔效应的影响.随着Fe含量的增加,体系的晶胞收缩,磁性逐渐降低.在Mn Al Ge中观测到高达~800Ω^(-1)cm^(-1)的反常霍尔电导率(AHC),并且随着Fe含量增加,体系的AHC逐渐降低.采用对数和TYJ模型对体系AHC的内外禀贡献进行定性和定量分离,其结果显示所有样品中内外禀机制对总的AHC贡献相当,并且两者均随着Fe含量的增加而降低.
