A reverse phase high performance liquid chromatographic method (HPLC) has been developed for the estimation of Alfuzosin hydrochloride (ALH) in the pharmaceutical formulation using RP-C18 column. The mobile phase (Tet...A reverse phase high performance liquid chromatographic method (HPLC) has been developed for the estimation of Alfuzosin hydrochloride (ALH) in the pharmaceutical formulation using RP-C18 column. The mobile phase (Tetrahydrofuran, Acetonitrile and buffer (pH 3.50) was pumped at a flow rate of 1.5 ml/min in the ratio of 1:20:80 and the eluents were monitored at 254.0 nm. Linearity was obtained in the concentration range of 80 - 120 μg/mL for ALH. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method may be used for determination of Alfuzosin hydrochloride in bulk drugs and in pharmaceutical dosage form.展开更多
We present a novel electrochemical sensor for the detection of alfuzosin(AFZ),an alpha1-adrenoceptor(α_(1)-AR)antagonist using metal-organic framework(MOF)-derived zinc oxide/cobalt oxide(ZnO/Co_(3)O_(4))decorated on...We present a novel electrochemical sensor for the detection of alfuzosin(AFZ),an alpha1-adrenoceptor(α_(1)-AR)antagonist using metal-organic framework(MOF)-derived zinc oxide/cobalt oxide(ZnO/Co_(3)O_(4))decorated on graphene(Gr).The co-precipitation method was utilized to synthesize a binary metal oxide derived from the MOF,and then ultrasonication was employed to prepare the ZnO/Co_(3)O_(4)@Gr nanocomposite.The prepared ZnO/Co_(3)O_(4)@Gr nanocomposite was characterized by X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),attenuated total reflectance-infrared(ATR-IR)spectroscopy,field emission scanning electron microscopy(FE-SEM),and high-resolution transmission electron microscopy(HR-TEM).The nanocomposite was modified on a glassy carbon electrode(GCE)to construct an electrochemical AFZ sensor,which exhibited a linear range of 0.05-40μM and a low limit of detection(LOD)of 0.004μM.The sensor showed good selectivity,as well as excellent reproducibility and storage stability.We also conducted the real sample analysis of the modified electrode for the detection of AFZ in pharmaceutical tablets,human serum,and urine samples,which delivered adequate recoveries.The ZnO/Co_(3)O_(4)@Gr nanocomposite-based electrochemical sensor is a promising candidate for the efficient and accurate detection of AFZ,owing to its superior sensitivity,selectivity,reproducibility,and stability.展开更多
The significance and diagnostic value of semen analysis in chronic bacterial prostatitis has been extensively debated and remains controversial. To investigate the diagnostic relevance of semen culture in the bacterio...The significance and diagnostic value of semen analysis in chronic bacterial prostatitis has been extensively debated and remains controversial. To investigate the diagnostic relevance of semen culture in the bacteriological workup of prostatitis patients, we retrospectively analyzed a clinical database of 696 symptomatic patients. All patients were routinely subjected to a four-glass test, followed by semen culture and analysis. This allowed to dissect from the database three different diagnostic scenarios, and to compare the ＇two-glass＇ pre-/post- massage test and the standard ＇four-glass＇ test with a ＇five-glass＇ test （four-glass plus post-VB3 semen culture）. The ＇five-glass＇ test showed 3.6- or 6.5-fold increases in relative sensitivity and lesser reductions （-13.2% or -14.7%） in relative specificity for traditional uropathogens （TUs） compared with the four-glass or two-glass test, respectively. The area under the ROC curve and Jouden＇s index were increased, whereas positive and negative likelihood ratios were lower than comparators, indicating that the ＇five-glass＇ assay may be superior in confirming the negative outcome of both standard tests. The five-, four-, and two-glass tests detected TUs （Enterobacteriaceae, Enterococci, etc.） in 120, 33, and 20 patients and unusual pathogens （Streptococci, other Gram-positive species, Mycoplasmata, and others） in 130, 56, and 45 patients, respectively. When patients were subjected to pharmacological treatment, including a combination of a fluoroquinolone and a macrolide, no differences in eradication rates were observed between groups diagnosed with different tests, irrespective of pathogen category. Eradication was associated with long-term sign/symptom remission; no significant intergroup differences in sign/symptom scores were observed throughout a 24-month off-therapy follow-up period. In conclusion, our data support the usefulness of semen analysis in the diagnostic workup ofprostatitis patients when this test is used to complement the four-glass Meares and Stamey test. Improvement of microbiological assays conveys important diagnostic and therapeutic implications.展开更多
文摘A reverse phase high performance liquid chromatographic method (HPLC) has been developed for the estimation of Alfuzosin hydrochloride (ALH) in the pharmaceutical formulation using RP-C18 column. The mobile phase (Tetrahydrofuran, Acetonitrile and buffer (pH 3.50) was pumped at a flow rate of 1.5 ml/min in the ratio of 1:20:80 and the eluents were monitored at 254.0 nm. Linearity was obtained in the concentration range of 80 - 120 μg/mL for ALH. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method may be used for determination of Alfuzosin hydrochloride in bulk drugs and in pharmaceutical dosage form.
基金supported by the Basic Science Research Program through the National Research Foundation of Korea(NRF),with a grant funded by the Korea Government(MSIT)(No.NRF-2020R1A2C1014918)the Korea Basic Science Institute(National Research Facilities and Equipment Center)grant funded by the Ministry of Education(2022R1A6C101B794)the MOE(NRF-2021R1A6A1A03039503)+1 种基金This work was conducted with the support of the Korea Environment Industry&Technology Institute(KEITI)through its Ecological Imitation-based Environmental Pollution Management Technology Development Project and by the Korea Ministry of Environment(ME)(2019002800006)supported by the Soonchunhyang University Research Fund.
文摘We present a novel electrochemical sensor for the detection of alfuzosin(AFZ),an alpha1-adrenoceptor(α_(1)-AR)antagonist using metal-organic framework(MOF)-derived zinc oxide/cobalt oxide(ZnO/Co_(3)O_(4))decorated on graphene(Gr).The co-precipitation method was utilized to synthesize a binary metal oxide derived from the MOF,and then ultrasonication was employed to prepare the ZnO/Co_(3)O_(4)@Gr nanocomposite.The prepared ZnO/Co_(3)O_(4)@Gr nanocomposite was characterized by X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),attenuated total reflectance-infrared(ATR-IR)spectroscopy,field emission scanning electron microscopy(FE-SEM),and high-resolution transmission electron microscopy(HR-TEM).The nanocomposite was modified on a glassy carbon electrode(GCE)to construct an electrochemical AFZ sensor,which exhibited a linear range of 0.05-40μM and a low limit of detection(LOD)of 0.004μM.The sensor showed good selectivity,as well as excellent reproducibility and storage stability.We also conducted the real sample analysis of the modified electrode for the detection of AFZ in pharmaceutical tablets,human serum,and urine samples,which delivered adequate recoveries.The ZnO/Co_(3)O_(4)@Gr nanocomposite-based electrochemical sensor is a promising candidate for the efficient and accurate detection of AFZ,owing to its superior sensitivity,selectivity,reproducibility,and stability.
文摘The significance and diagnostic value of semen analysis in chronic bacterial prostatitis has been extensively debated and remains controversial. To investigate the diagnostic relevance of semen culture in the bacteriological workup of prostatitis patients, we retrospectively analyzed a clinical database of 696 symptomatic patients. All patients were routinely subjected to a four-glass test, followed by semen culture and analysis. This allowed to dissect from the database three different diagnostic scenarios, and to compare the ＇two-glass＇ pre-/post- massage test and the standard ＇four-glass＇ test with a ＇five-glass＇ test （four-glass plus post-VB3 semen culture）. The ＇five-glass＇ test showed 3.6- or 6.5-fold increases in relative sensitivity and lesser reductions （-13.2% or -14.7%） in relative specificity for traditional uropathogens （TUs） compared with the four-glass or two-glass test, respectively. The area under the ROC curve and Jouden＇s index were increased, whereas positive and negative likelihood ratios were lower than comparators, indicating that the ＇five-glass＇ assay may be superior in confirming the negative outcome of both standard tests. The five-, four-, and two-glass tests detected TUs （Enterobacteriaceae, Enterococci, etc.） in 120, 33, and 20 patients and unusual pathogens （Streptococci, other Gram-positive species, Mycoplasmata, and others） in 130, 56, and 45 patients, respectively. When patients were subjected to pharmacological treatment, including a combination of a fluoroquinolone and a macrolide, no differences in eradication rates were observed between groups diagnosed with different tests, irrespective of pathogen category. Eradication was associated with long-term sign/symptom remission; no significant intergroup differences in sign/symptom scores were observed throughout a 24-month off-therapy follow-up period. In conclusion, our data support the usefulness of semen analysis in the diagnostic workup ofprostatitis patients when this test is used to complement the four-glass Meares and Stamey test. Improvement of microbiological assays conveys important diagnostic and therapeutic implications.
