Trimethylation of histone H3K4(H3K4me3)is widely distributed at numerous actively transcribed protein-coding genes throughout the genome.However,the interplay between H3K4me3 and other chromatin modifications in plant...Trimethylation of histone H3K4(H3K4me3)is widely distributed at numerous actively transcribed protein-coding genes throughout the genome.However,the interplay between H3K4me3 and other chromatin modifications in plants remains poorly understood.In this study,we show that the Arabidopsis thaliana ALFIN-LIKE(AL)proteins contain a C-terminal PHD finger capable of binding to H3K4me3 and a PHD-associated AL(PAL)domain that interacts with components of the Polycomb repressive complex 1,thereby facilitating H2A ubiquitination(H2Aub)at H3K4me3-enriched genes throughout the genome.Furthermore,we demonstrate that loss of function of SDG2,encoding a key histone H3K4 methyltransferase,leads to a reduction in H3K4me3 level,which subsequently causes a genome-wide decrease in H2Aub,revealing a strong association between H3K4me3 and H2Aub.Finally,we discover that the PAL domain of AL proteins interacts with various other chromatin-related proteins or complexes,including those involved in regulating H2A.Z deposition,H3K27me3 demethylation,histone deacetylation,and chromatin accessibility.Our genome-wide analysis suggests that the AL proteins play a crucial role in coordinating H3K4me3 with multiple other chromatin modifications across the genome.展开更多
基金supported by the National Natural Science Foundation of China to X.-J.H.(grant numbers 32025003 and 32470628).
文摘Trimethylation of histone H3K4(H3K4me3)is widely distributed at numerous actively transcribed protein-coding genes throughout the genome.However,the interplay between H3K4me3 and other chromatin modifications in plants remains poorly understood.In this study,we show that the Arabidopsis thaliana ALFIN-LIKE(AL)proteins contain a C-terminal PHD finger capable of binding to H3K4me3 and a PHD-associated AL(PAL)domain that interacts with components of the Polycomb repressive complex 1,thereby facilitating H2A ubiquitination(H2Aub)at H3K4me3-enriched genes throughout the genome.Furthermore,we demonstrate that loss of function of SDG2,encoding a key histone H3K4 methyltransferase,leads to a reduction in H3K4me3 level,which subsequently causes a genome-wide decrease in H2Aub,revealing a strong association between H3K4me3 and H2Aub.Finally,we discover that the PAL domain of AL proteins interacts with various other chromatin-related proteins or complexes,including those involved in regulating H2A.Z deposition,H3K27me3 demethylation,histone deacetylation,and chromatin accessibility.Our genome-wide analysis suggests that the AL proteins play a crucial role in coordinating H3K4me3 with multiple other chromatin modifications across the genome.
