本文旨在对ALA-PDT (5-氨基酮戊酸光动力治疗)在治疗女性下生殖道疾病合并HPV感染中的临床疗效及作用机制进行综述。近年来,ALA-PDT因其微无创、可重复治疗、保留器官功能等优点逐渐受到临床重视。本文总结了ALA-PDT在女性下生殖道疾病...本文旨在对ALA-PDT (5-氨基酮戊酸光动力治疗)在治疗女性下生殖道疾病合并HPV感染中的临床疗效及作用机制进行综述。近年来,ALA-PDT因其微无创、可重复治疗、保留器官功能等优点逐渐受到临床重视。本文总结了ALA-PDT在女性下生殖道疾病合并HPV感染的治疗效果,此外结合国内外已有研究,对ALA-PDT对于肿瘤细胞增殖与凋亡、免疫微环境、肿瘤微环境的影响进行总结分析。研究表明,ALA-PDT在治疗女性下生殖道HPV感染及其相关病变中具有显著疗效,并且相较于传统治疗方式,具有保留器官结构以及功能完整性的优势。未来的研究应制定更加个体化的治疗方案,并探讨其与其他治疗方式的联合使用,以期提高临床疗效和降低疾病复发率。This article aims to review the clinical efficacy and mechanism of ALA-PDT (5-aminolevulinic acid photodynamic therapy) in the treatment of female lower reproductive tract diseases complicated with HPV infection. In recent years, ALA-PDT has been paid more and more attention in clinic because of its advantages of minimal invasion, repeatable treatment and preservation of organ function. In this paper, the therapeutic effect of ALA-PDT on female lower reproductive tract diseases complicated with HPV infection was summarized. In addition, the effects of ALA-PDT on tumor cell proliferation and apoptosis, immune microenvironment and tumor microenvironment were summarized and analyzed. Studies have shown that ALA-PDT has a significant effect in the treatment of HPV infection and related diseases in female lower reproductive tract, and compared with traditional treatment methods, it has the advantage of preserving organ structure and functional integrity. Future research should formulate more individualized treatment plans and explore their combined use with other treatment methods in order to improve clinical efficacy and reduce the recurrence rate of diseases.展开更多
文摘本文旨在对ALA-PDT (5-氨基酮戊酸光动力治疗)在治疗女性下生殖道疾病合并HPV感染中的临床疗效及作用机制进行综述。近年来,ALA-PDT因其微无创、可重复治疗、保留器官功能等优点逐渐受到临床重视。本文总结了ALA-PDT在女性下生殖道疾病合并HPV感染的治疗效果,此外结合国内外已有研究,对ALA-PDT对于肿瘤细胞增殖与凋亡、免疫微环境、肿瘤微环境的影响进行总结分析。研究表明,ALA-PDT在治疗女性下生殖道HPV感染及其相关病变中具有显著疗效,并且相较于传统治疗方式,具有保留器官结构以及功能完整性的优势。未来的研究应制定更加个体化的治疗方案,并探讨其与其他治疗方式的联合使用,以期提高临床疗效和降低疾病复发率。This article aims to review the clinical efficacy and mechanism of ALA-PDT (5-aminolevulinic acid photodynamic therapy) in the treatment of female lower reproductive tract diseases complicated with HPV infection. In recent years, ALA-PDT has been paid more and more attention in clinic because of its advantages of minimal invasion, repeatable treatment and preservation of organ function. In this paper, the therapeutic effect of ALA-PDT on female lower reproductive tract diseases complicated with HPV infection was summarized. In addition, the effects of ALA-PDT on tumor cell proliferation and apoptosis, immune microenvironment and tumor microenvironment were summarized and analyzed. Studies have shown that ALA-PDT has a significant effect in the treatment of HPV infection and related diseases in female lower reproductive tract, and compared with traditional treatment methods, it has the advantage of preserving organ structure and functional integrity. Future research should formulate more individualized treatment plans and explore their combined use with other treatment methods in order to improve clinical efficacy and reduce the recurrence rate of diseases.
文摘目的:检测5-氨基酮戊酸-光动力疗法(ALA-PDT)治疗前后尖锐湿疣皮损凋亡相关因子(Fas、caspase3、Bcl-2)及促炎症细胞因子(CCL-20、TNF-α、IL-1)的表达,探讨AL A-PDT治疗尖锐湿疣的可能机理。方法:收集10例尖锐湿疣患者ALA-PDT治疗前后皮损(同时以正常健康者皮肤组织对照),采用荧光定量PCR检测其Fas、caspase3、Bcl-2、CCL-20、TNF-α、IL-1 mRNA表达;Western blot检测其Fas、caspase3、Bcl-2、CCL-20、TNF-α、IL-1蛋白表达。结果:尖锐湿疣皮损组织内Fas、caspase3、Bcl-2、CCL-20、TNF-α、IL-1 mRNA和蛋白表达均高于正常皮肤组织(P<0.05);与治疗前比较,治疗后Bcl-2、CCL-20、TNF-α、IL-1 m RNA和蛋白表达水平明显降低(P<0.01),Fas、caspase3 m RNA和蛋白表达水平明显升高(P<0.01)。结论:AL A-PDT是一种能有效治疗尖锐湿疣的方法,它可诱导细胞表面凋亡蛋白Fas、caspase3的表达、减少凋亡抑制因子Bcl-2的表达,从而可能依靠内源性途径(线粒体介导的凋亡)和外源性途径(死亡受体介导的凋亡)促进尖锐湿疣角质形成细胞凋亡;并可能通过抑制促炎细胞因子CCL-20、TNF-α、IL-1而发挥局部免疫调节作用。
