目的 比较肝癌患者自体 CD3AK细胞和 L AK细胞的杀伤活性 ,为临床应用提供依据。方法 以流式细胞仪检测细胞的杀伤活性。结果 原发性肝癌患者自体的 CD3AK细胞的 NK活性和 L AK活性均高于 L AK细胞。结论 CD3AK细胞效果稳定 ,抗瘤...目的 比较肝癌患者自体 CD3AK细胞和 L AK细胞的杀伤活性 ,为临床应用提供依据。方法 以流式细胞仪检测细胞的杀伤活性。结果 原发性肝癌患者自体的 CD3AK细胞的 NK活性和 L AK活性均高于 L AK细胞。结论 CD3AK细胞效果稳定 ,抗瘤效应明显高于 L AK细胞 ,CD3AK细胞可望作为一种新的生物治疗手段用于肿瘤治疗。展开更多
Multiple morphological abnormalities of the flagella(MMAF)represent a severe form of sperm defects leading to asthenozoospermia and male infertility.In this study,we identified a novel homozygous splicing mutation(c.8...Multiple morphological abnormalities of the flagella(MMAF)represent a severe form of sperm defects leading to asthenozoospermia and male infertility.In this study,we identified a novel homozygous splicing mutation(c.871-4 ACA>A)in the adenylate kinase 7(AK7)gene by whole-exome sequencing in infertile individuals.Spermatozoa from affected individuals exhibited typical MMAF characteristics,including coiled,bent,short,absent,and irregular flagella.Transmission electron microscopy analysis showed disorganized axonemal structure and abnormal mitochondrial sheets in sperm flagella.Immunofluorescence staining confirmed the absence of AK7 protein from the patients’spermatozoa,validating the pathogenic nature of the mutation.This study provides direct evidence linking the AK7 gene to MMAF-associated asthenozoospermia in humans,expanding the mutational spectrum of AK7 and enhancing our understanding of the genetic basis of male infertility.展开更多
Aimed at the problem of the low computational efficiency of the existing urethral-valve reliability analysis,an efficient reliability analysis method of the wireless energy-transmitting urethral-valve(WETUV)was propos...Aimed at the problem of the low computational efficiency of the existing urethral-valve reliability analysis,an efficient reliability analysis method of the wireless energy-transmitting urethral-valve(WETUV)was proposed.The method is called FTA-AK-SS,based on the active learning Kriging(AK)model,subset simulation(SS)algorithm,and fault tree analysis(FTA).According to the principle of FTA,we established the fault tree model of the WETUV to determine its minimum cut set and bottom events.Then we defined the random variables affecting its reliability.The U learning function was used to selectively add the sample points of random variables to update the initial Kriging surrogate model.At the same time,combined with the SS algorithm,the reliability and sensitivity analyses of the WETUV were realized.The result shows that compared with the traditional Monte Carlo simulation and FTA-Kriging-SS methods,the proposed method significantly improves the calculation efficiency of the WETUV under the premise of ensuring calculation accuracy.The reliability of the WETUV is greatly affected by the rubber pad’s aging,the receiver coil’s corrosion,and the position deviation.This study can provide a new way to realize a high-efficiency reliability calculation and analysis for urethral valves.展开更多
Medically assisted reproduction(MAR)techniques are highly dependent on the sperm quantity and quality.Low sperm concentrations can be bypassed at least to some point by the usage of more sophisticated MAR techniques l...Medically assisted reproduction(MAR)techniques are highly dependent on the sperm quantity and quality.Low sperm concentrations can be bypassed at least to some point by the usage of more sophisticated MAR techniques like intracytoplasmic sperm injection(ICSI).Compared to this,disruptions in established indicators of sperm quality like motility or morphology pose greater challenges for the therapy of couple infertility.展开更多
文摘目的 比较肝癌患者自体 CD3AK细胞和 L AK细胞的杀伤活性 ,为临床应用提供依据。方法 以流式细胞仪检测细胞的杀伤活性。结果 原发性肝癌患者自体的 CD3AK细胞的 NK活性和 L AK活性均高于 L AK细胞。结论 CD3AK细胞效果稳定 ,抗瘤效应明显高于 L AK细胞 ,CD3AK细胞可望作为一种新的生物治疗手段用于肿瘤治疗。
基金supported by the National Key Research and Development Program of China(No.2021YFC2700202,No.2022YFA0806303,and No.2022YFC2702601)the Global Select Project of the Institute of Health and Medicine,Hefei Comprehensive National Science Center(DJK-LX-2022010)the Joint Fund for New Medicine of USTC(YD9100002034).
文摘Multiple morphological abnormalities of the flagella(MMAF)represent a severe form of sperm defects leading to asthenozoospermia and male infertility.In this study,we identified a novel homozygous splicing mutation(c.871-4 ACA>A)in the adenylate kinase 7(AK7)gene by whole-exome sequencing in infertile individuals.Spermatozoa from affected individuals exhibited typical MMAF characteristics,including coiled,bent,short,absent,and irregular flagella.Transmission electron microscopy analysis showed disorganized axonemal structure and abnormal mitochondrial sheets in sperm flagella.Immunofluorescence staining confirmed the absence of AK7 protein from the patients’spermatozoa,validating the pathogenic nature of the mutation.This study provides direct evidence linking the AK7 gene to MMAF-associated asthenozoospermia in humans,expanding the mutational spectrum of AK7 and enhancing our understanding of the genetic basis of male infertility.
基金Foundation item:the National Natural Science Foundation of China(No.52075101)the Natural Science Foundation of Guangdong Province(No.2017A030313265)Guangzhou Scientific Research Plan(No.201904010184)。
文摘Aimed at the problem of the low computational efficiency of the existing urethral-valve reliability analysis,an efficient reliability analysis method of the wireless energy-transmitting urethral-valve(WETUV)was proposed.The method is called FTA-AK-SS,based on the active learning Kriging(AK)model,subset simulation(SS)algorithm,and fault tree analysis(FTA).According to the principle of FTA,we established the fault tree model of the WETUV to determine its minimum cut set and bottom events.Then we defined the random variables affecting its reliability.The U learning function was used to selectively add the sample points of random variables to update the initial Kriging surrogate model.At the same time,combined with the SS algorithm,the reliability and sensitivity analyses of the WETUV were realized.The result shows that compared with the traditional Monte Carlo simulation and FTA-Kriging-SS methods,the proposed method significantly improves the calculation efficiency of the WETUV under the premise of ensuring calculation accuracy.The reliability of the WETUV is greatly affected by the rubber pad’s aging,the receiver coil’s corrosion,and the position deviation.This study can provide a new way to realize a high-efficiency reliability calculation and analysis for urethral valves.
文摘Medically assisted reproduction(MAR)techniques are highly dependent on the sperm quantity and quality.Low sperm concentrations can be bypassed at least to some point by the usage of more sophisticated MAR techniques like intracytoplasmic sperm injection(ICSI).Compared to this,disruptions in established indicators of sperm quality like motility or morphology pose greater challenges for the therapy of couple infertility.
