Objective To contrastively observe the effect of electroacupuncture at lower he-sea points of stomach, large intestine, small intestine and gallbladder of acute gastric mucosal lesion（AGML） model rats on the express...Objective To contrastively observe the effect of electroacupuncture at lower he-sea points of stomach, large intestine, small intestine and gallbladder of acute gastric mucosal lesion（AGML） model rats on the expression of serum high mobility group protein B 1（HMGB 1）, tumor necrosis factor-α（TNF-α） and nuclear factor-κB（NF-κB） of gastric tissue, and to explore whether the lower he-sea points of stomach have relative specificity in treatment of stomach diseases. Methods Seventy healthy SD rats were randomly divided into seven groups, including control group, model group, ＂Zúsānlǐ＂（足三里 ST 36） group, ＂Shàngjùxū＂（上巨虚 ST 37） group, ＂Xiàjùxū＂（下巨虚 ST 39） group, ＂Yánglíngquán＂（阳陵泉 GB 34） group and ＂Chéngjīn＂（承筋 BL 56） group, with five male rats and five female rats in each group. Acute gastric mucosal lesion model was established by applying WRS method. After successful modeling and treatment for ten days, sampling was conducted, gastric mucosal lesion was observed visually and scored, the levels of serum HMGB 1 and gastric tissue TNF-α were detected by ELISA method, and the expression of NF-κB was detected by immunohistochemical method. Results①Compared with the model group, the levels of Ulcers and inflammation（UI） in gastric mucosal and serum HMGB1, and the expression of gastric tissue TNF-α and NF-κB in the four lower he-sea points groups reduced（P〈0.01 or P〈0.05）;② compared with ST 36 group, the levels of gastric mucosal UI and serum HMGB1, and the expression of gastric mucosal TNF-α and NF-κB in other treatment groups increased significantly, and the difference was statistically significant（P〈0.01 or P〈0.05）. Conclusion①Electroacupuncture at relevant lower he-sea points can inhibit the body＇s immune response, relieve inflammatory reaction and reduce mucosal lesion by regulating HMGB 1, TNF-α and NF-κB, so as to realize the intervention and treatment on AGML rats;② the total intervention effect in ST 36 group is superior to that in other groups, indicating partially that there is a relative specificity between ST 36 and stomach.展开更多
基金Supported by:National Natural Science Foundation of China(81173327,81303048)
文摘Objective To contrastively observe the effect of electroacupuncture at lower he-sea points of stomach, large intestine, small intestine and gallbladder of acute gastric mucosal lesion（AGML） model rats on the expression of serum high mobility group protein B 1（HMGB 1）, tumor necrosis factor-α（TNF-α） and nuclear factor-κB（NF-κB） of gastric tissue, and to explore whether the lower he-sea points of stomach have relative specificity in treatment of stomach diseases. Methods Seventy healthy SD rats were randomly divided into seven groups, including control group, model group, ＂Zúsānlǐ＂（足三里 ST 36） group, ＂Shàngjùxū＂（上巨虚 ST 37） group, ＂Xiàjùxū＂（下巨虚 ST 39） group, ＂Yánglíngquán＂（阳陵泉 GB 34） group and ＂Chéngjīn＂（承筋 BL 56） group, with five male rats and five female rats in each group. Acute gastric mucosal lesion model was established by applying WRS method. After successful modeling and treatment for ten days, sampling was conducted, gastric mucosal lesion was observed visually and scored, the levels of serum HMGB 1 and gastric tissue TNF-α were detected by ELISA method, and the expression of NF-κB was detected by immunohistochemical method. Results①Compared with the model group, the levels of Ulcers and inflammation（UI） in gastric mucosal and serum HMGB1, and the expression of gastric tissue TNF-α and NF-κB in the four lower he-sea points groups reduced（P〈0.01 or P〈0.05）;② compared with ST 36 group, the levels of gastric mucosal UI and serum HMGB1, and the expression of gastric mucosal TNF-α and NF-κB in other treatment groups increased significantly, and the difference was statistically significant（P〈0.01 or P〈0.05）. Conclusion①Electroacupuncture at relevant lower he-sea points can inhibit the body＇s immune response, relieve inflammatory reaction and reduce mucosal lesion by regulating HMGB 1, TNF-α and NF-κB, so as to realize the intervention and treatment on AGML rats;② the total intervention effect in ST 36 group is superior to that in other groups, indicating partially that there is a relative specificity between ST 36 and stomach.
