拟南芥AFPs (ABI five interaction proteins)是一类与植物激素脱落酸(ABA)信号途径中转录因子ABI5互作的蛋白,以前报道它们可以通过促进ABI5的降解来调控种子萌发,但是AFPs的新功能鲜有报道。我们发现过表达AFPs家族成员之一AFP2可以...拟南芥AFPs (ABI five interaction proteins)是一类与植物激素脱落酸(ABA)信号途径中转录因子ABI5互作的蛋白,以前报道它们可以通过促进ABI5的降解来调控种子萌发,但是AFPs的新功能鲜有报道。我们发现过表达AFPs家族成员之一AFP2可以促进拟南芥小苗茉莉酸(JA)的合成并延迟开花,反之,拟南芥突变体afp2表现为较低的茉莉酸积累与较早的开花时间。突变茉莉酸合成酶LOX2或受体COI1都可以部分缓解AFP2-ox的晚花表型,同时过表达AFP2增强了AFP2类转录因子TOE1结合到成花素FT启动子区域的能力,并抑制FT的转录导致晚花。以上结果揭示了AFP2参与ABA与JA信号调控开花时间的新功能。展开更多
Ambient temperature induces the hypocotyl elongation of seedling,called as thermomorphogenesis.It has been reported that the bHLH transcriptional factor PIF7 acts as the critical component to modulate plant thermomorp...Ambient temperature induces the hypocotyl elongation of seedling,called as thermomorphogenesis.It has been reported that the bHLH transcriptional factor PIF7 acts as the critical component to modulate plant thermomorphogenesis,but the underlying mechanism remains elusive.The phytohormone abscisic acid(ABA)suppresses the hypocotyl elongation under high temperature(HT)stress.As the ABI5 binding protein,AFP2 acts as the negative factor to control ABA signaling.In this study,we first identified AFP2 as the interaction protein of PIF7 in vitro and in vivo.Phenotype analysis revealed that overexpressing AFP2 reduced the hypocotyl elongation,while loss-of-function afp2 mutant showed longer hypocotyl under HT.Consistently,overexpressing AFP2 impaired the transactivation effect of PIF7 on auxin biosynthesis related genes YUC8 and IAA19,which possibly resulted into the shorter hypocotyl in the transgenic line overexpressing AFP2 or co-overexpressing AFP2 and PIF7.Thus,these data suggest that AFP2 suppressed PIF7 activity to suppress hypocotyl elongation.Furthermore,we found that HT gradually induced the degradation of AFP2 that possibly released the inhibitory effect of AFP2 on PIF7,thus induced hypocotyl elongation under HT.Taken together,our result reveals the novel function of AFP2 in coordinating thermomorphogenesis through sophistically modulating PIF7 activity.展开更多
文摘拟南芥AFPs (ABI five interaction proteins)是一类与植物激素脱落酸(ABA)信号途径中转录因子ABI5互作的蛋白,以前报道它们可以通过促进ABI5的降解来调控种子萌发,但是AFPs的新功能鲜有报道。我们发现过表达AFPs家族成员之一AFP2可以促进拟南芥小苗茉莉酸(JA)的合成并延迟开花,反之,拟南芥突变体afp2表现为较低的茉莉酸积累与较早的开花时间。突变茉莉酸合成酶LOX2或受体COI1都可以部分缓解AFP2-ox的晚花表型,同时过表达AFP2增强了AFP2类转录因子TOE1结合到成花素FT启动子区域的能力,并抑制FT的转录导致晚花。以上结果揭示了AFP2参与ABA与JA信号调控开花时间的新功能。
基金funded by the National Natural Science Foundation of China(Grant No.31970289).
文摘Ambient temperature induces the hypocotyl elongation of seedling,called as thermomorphogenesis.It has been reported that the bHLH transcriptional factor PIF7 acts as the critical component to modulate plant thermomorphogenesis,but the underlying mechanism remains elusive.The phytohormone abscisic acid(ABA)suppresses the hypocotyl elongation under high temperature(HT)stress.As the ABI5 binding protein,AFP2 acts as the negative factor to control ABA signaling.In this study,we first identified AFP2 as the interaction protein of PIF7 in vitro and in vivo.Phenotype analysis revealed that overexpressing AFP2 reduced the hypocotyl elongation,while loss-of-function afp2 mutant showed longer hypocotyl under HT.Consistently,overexpressing AFP2 impaired the transactivation effect of PIF7 on auxin biosynthesis related genes YUC8 and IAA19,which possibly resulted into the shorter hypocotyl in the transgenic line overexpressing AFP2 or co-overexpressing AFP2 and PIF7.Thus,these data suggest that AFP2 suppressed PIF7 activity to suppress hypocotyl elongation.Furthermore,we found that HT gradually induced the degradation of AFP2 that possibly released the inhibitory effect of AFP2 on PIF7,thus induced hypocotyl elongation under HT.Taken together,our result reveals the novel function of AFP2 in coordinating thermomorphogenesis through sophistically modulating PIF7 activity.
