目的:检测肝细胞肝癌(hepatocellular carcinoma,HCC)癌组织、癌旁组织(adjacent normal liver tissue,ANLT)及不同侵袭能力细胞系中极性蛋白AF-6 m RNA的表达情况,分析其在不同组织及不同细胞系中的表达差异及意义.方法:利用实时定量PC...目的:检测肝细胞肝癌(hepatocellular carcinoma,HCC)癌组织、癌旁组织(adjacent normal liver tissue,ANLT)及不同侵袭能力细胞系中极性蛋白AF-6 m RNA的表达情况,分析其在不同组织及不同细胞系中的表达差异及意义.方法:利用实时定量PCR检测(real-time quantitative,q RT-PCR)检测AF-6 m RNA在3 0对癌组织、A N LT和4种细胞系(L 0 2、Hep G2、MHCC97-H和HCCLM3)中的表达情况.结果:AF-6 m RNA在93.3%(28/30)的HCC中呈低表达;正常肝细胞株L02中AF-6 m RNA含量明显高于肝癌细胞株(P<0.05);高侵袭转移能力的细胞系MHCC97-H及HCCLM3只有极低的AF-6 m RNA表达,且明显低于低侵袭转移能力的细胞系Hep G2(P<0.05).结论:AF-6 m RNA在肝癌中的低表达可能与高侵袭能力相关,提示AF-6 m RNA在将来可能会成为治疗侵袭性HCC的潜在靶点.展开更多
Now the six-axis force sensor (6-AFS) is used widely, and as the core components, mechanical properties of its elastic bodies are significant. With the increase of dynamic loads, studies on dynamic characteristics of ...Now the six-axis force sensor (6-AFS) is used widely, and as the core components, mechanical properties of its elastic bodies are significant. With the increase of dynamic loads, studies on dynamic characteristics of the 6-AFS become more and more important. In this paper, the study focuses on the free vibration problem of a novel 6-AFS. The research approach is to decompose the sensor into several separate elastic bodies (four lamellas and upper and lower membranes) and research these elastic bodies respectively. The free vibration of the lamella is studied based on Rayleigh-Ritz method and the separation of variables. The analytical solutions of free vibration of the membranes are deduced according to the nature of Bessel functions. Both the analytical results are simulated with MATLAB. Compared the simulated diagrams with actual situations, they are very close. The mode shapes obtained play a major role in solving the forced vibration of the sensor.展开更多
文摘目的:检测肝细胞肝癌(hepatocellular carcinoma,HCC)癌组织、癌旁组织(adjacent normal liver tissue,ANLT)及不同侵袭能力细胞系中极性蛋白AF-6 m RNA的表达情况,分析其在不同组织及不同细胞系中的表达差异及意义.方法:利用实时定量PCR检测(real-time quantitative,q RT-PCR)检测AF-6 m RNA在3 0对癌组织、A N LT和4种细胞系(L 0 2、Hep G2、MHCC97-H和HCCLM3)中的表达情况.结果:AF-6 m RNA在93.3%(28/30)的HCC中呈低表达;正常肝细胞株L02中AF-6 m RNA含量明显高于肝癌细胞株(P<0.05);高侵袭转移能力的细胞系MHCC97-H及HCCLM3只有极低的AF-6 m RNA表达,且明显低于低侵袭转移能力的细胞系Hep G2(P<0.05).结论:AF-6 m RNA在肝癌中的低表达可能与高侵袭能力相关,提示AF-6 m RNA在将来可能会成为治疗侵袭性HCC的潜在靶点.
文摘Now the six-axis force sensor (6-AFS) is used widely, and as the core components, mechanical properties of its elastic bodies are significant. With the increase of dynamic loads, studies on dynamic characteristics of the 6-AFS become more and more important. In this paper, the study focuses on the free vibration problem of a novel 6-AFS. The research approach is to decompose the sensor into several separate elastic bodies (four lamellas and upper and lower membranes) and research these elastic bodies respectively. The free vibration of the lamella is studied based on Rayleigh-Ritz method and the separation of variables. The analytical solutions of free vibration of the membranes are deduced according to the nature of Bessel functions. Both the analytical results are simulated with MATLAB. Compared the simulated diagrams with actual situations, they are very close. The mode shapes obtained play a major role in solving the forced vibration of the sensor.
