Background Evidence on the effects of different exercise interventions on cognitive function is insufficient.Aims To evaluate the feasibility and effects of remotely supervised aerobic exercise(AE)and resistance exerc...Background Evidence on the effects of different exercise interventions on cognitive function is insufficient.Aims To evaluate the feasibility and effects of remotely supervised aerobic exercise(AE)and resistance exercise(RE)interventions in older adults with mild cognitive impairment(MCI).Methods This study is a 6-month pilot three-arm randomised controlled trial.Eligible participants(n=108)were recruited and randomised to the AE group,RE group or control(CON)group with a 1:1:1 ratio.Interventions were delivered at home with remote supervision.We evaluated participants’global cognition,memory,executive function,attention,physical activity levels,physical performance and muscle strength of limbs at baseline,3 months(T1)and 6 months(T2)after randomisation.A linear mixed-effects model was adopted for data analyses after controlling for covariates.Tukey’s method was used for adjusting for multiple comparisons.Sensitivity analyses were performed after excluding individuals with low compliance rates.Results 15(13.89%)participants dropped out.The median compliance rates in the AE group and RE group were 67.31%and 93.27%,respectively.After adjusting for covariates,the scores of the Alzheimer’s Disease Assessment Scale-Cognitive subscale in the AE group decreased by 2.04(95%confidence interval(CI)−3.41 to−0.67,t=−2.94,p=0.004)and 1.53(95%CI−2.88 to−0.17,t=−2.22,p=0.028)points more than those in the CON group at T1 and T2,respectively.The effects of AE were still significant at T1(estimate=−1.70,95%CI−3.20 to−0.21,t=−2.69,p=0.021),but lost statistical significance at T2 after adjusting for multiple comparisons.As for executive function,the Stroop time interference in the RE group decreased by 11.76 s(95%CI−21.62 to−1.90,t=−2.81,p=0.015)more than that in the AE group at T2 after Tukey’s adjustment.No other significant effects on cognitive functions were found.Conclusions Both remotely supervised AE and RE programmes are feasible in older adults with MCI.AE has positive effects on global cognition,and RE improves executive function.展开更多
A visible-light-enabled method for the synthesis ofα-azidoketones has been developed via oxo-azidation of alkenyl silanes with trimethylsilylazide and molecular oxygen under mild conditions.The reaction could be carr...A visible-light-enabled method for the synthesis ofα-azidoketones has been developed via oxo-azidation of alkenyl silanes with trimethylsilylazide and molecular oxygen under mild conditions.The reaction could be carried out in gram scale.Various radical sources,including trifluoromethyl radical,thiocyanate radical,bromide radical,chlorine radical could partici-pate effectively instead of azide radical in the reaction.展开更多
[Objectives]To synthesize evidence on HIIT versus moderate-intensity continuous training(MICT)or routine rehabilitation in stroke survivors.[Methods]We systematically searched 8 databases(PubMed,EMBASE,CENTRAL,Web of ...[Objectives]To synthesize evidence on HIIT versus moderate-intensity continuous training(MICT)or routine rehabilitation in stroke survivors.[Methods]We systematically searched 8 databases(PubMed,EMBASE,CENTRAL,Web of Science,SPORTSDiscus,PsycINFO,SCOPUS,CINAHL)up to May 2025.Seventeen randomized controlled trials(RCTs;total n=1142)met inclusion criteria:adults with stroke,device-based HIIT(≥70%HRR/VO 2peak),and outcomes assessing VO 2peak,6-min walk distance(6MWD),or Berg Balance Scale(BBS).Methodological quality was evaluated using the PEDro scale.Pooled effect sizes(Hedges'g)were calculated via random-effects models,with heterogeneity quantified by I^(2).[Results]HIIT significantly improved peak oxygen uptake(VO 2peak)versus controls(g=0.59,95%CI:0.44-0.75,p<0.001;I^(2)=16.29%).Low heterogeneity and symmetrical funnel plots supported robustness.HIIT also enhanced walking endurance(6MWD:g=0.32,95%CI:0.16-0.48,p<0.01;I^(2)=30%).In contrast,no significant benefit was observed for balance function(BBS:g=0.07,95%CI:-0.13-0.26,p=0.50;I^(2)=0%).[Conclusions]HIIT is a safe and highly effective intervention for enhancing aerobic capacity and walking function post-stroke.Its benefits are maximized at higher intensities and longer durations but do not extend to balance improvement.Integrating HIIT into stroke rehabilitation protocols is strongly recommended to promote functional independence.展开更多
Objectives:Aerobic exercise training can increase skeletal muscle mitochondrial content.Supine exercise training with legs above the heart potentially augments these increases.However,the impact of supine exercise tra...Objectives:Aerobic exercise training can increase skeletal muscle mitochondrial content.Supine exercise training with legs above the heart potentially augments these increases.However,the impact of supine exercise training on mitochondrial biogenesis and cardiovascular adaptations remains unclear.Methods:In this single-centred,randomized,parallel arm trial,19 recreationally active individuals underwent seven sessions of either supine with legs up(SUP;n=9,6 females)or upright with legs down(UP;n=10,7 females)aerobic training on a recumbent bike at 71±7%and 71±2%of peak work rate(WRpeak),respectively.The study aimed to test the effects of training with decreased muscle oxygenation on indices of muscle mitochondrial remodelling.Secondary outcomes included exercise performance,muscle oxygenation,and cardiovascular responses.Results:Secondary outcomes revealed significant interaction effects for time to fatigue(TTF)and WRpeak in the SUP group during supine testing,suggesting enhanced exercise tolerance and performance.No between group interaction effects were observed for upright testing.No clear effects on mitochondrial biogenesis were observed based on expression of mitochondrial protein subunits and transcriptional regulators.Acutely,HRpeak was lower during the SUP Test compared to the UP Test.No central cardiovascular adaptations were observed following training.Conclusions:Our exploratory analyses showed that supine aerobic training more effectively improves supine exercise tolerance and performance compared with upright training,despite no differences in measured proteins related to mitochondrial biogenesis.Further research is needed to elucidate the mechanisms underlying these postural-specific training effects.Registration:clinicaltrials.gov:NCT04151095.展开更多
This work established a quantitative method to access the shear stability of aerobic granular sludge(AGS)and validated its feasibility by using the mature AGS from a pilot-scale(50 tons/day)membrane bioreactor(MBR)for...This work established a quantitative method to access the shear stability of aerobic granular sludge(AGS)and validated its feasibility by using the mature AGS from a pilot-scale(50 tons/day)membrane bioreactor(MBR)for treating real municipal wastewater.The results showed that the changing rate( S)of the peak area(S)of granule size distribution(GSD)exhibited an exponential relationship(R^(2)≥0.76)with the shear time(y=a−b·c^(x)),which was a suitable indicative index to reflect the shear stability of different AGS samples.The limiting granule size(LGS)was defined and proposed to characterize the equilibrium size for AGS after being sheared for a period of time,whose value in terms of Dv50 showed high correlation(R^(2)=0.92)with the parameter a.The free Ca^(2+)(28.44-34.21 mg/L)in the influent specifically interacted with polysaccharides(PS)in the granule’s extracellular polymeric substance(EPS)as a nucleation site,thereby inducing the formation of Ca precipitation to enhance its Young’s modulus,while Ca^(2+) primarily interacted with PS in soluble metabolic product(SMP)during the initial granulation process.Furthermore,the Young’s modulus significantly affected the parameter a related to shear stability(R^(2)=0.99).Since the parameter a was more closely related(R^(2)=1.00)to S than that of the parameter b or c,the excellent correlation(R^(2)=0.99)between the parameter a and the wet density further verified the feasibility of this method.展开更多
Background:Statins are the cornerstone of low-density lipoprotein cholesterol(LDL-C)-lowering therapy;however,the therapeutic efficacy of statins in countering atherosclerotic cardiovascular disease(ASCVD)is compromis...Background:Statins are the cornerstone of low-density lipoprotein cholesterol(LDL-C)-lowering therapy;however,the therapeutic efficacy of statins in countering atherosclerotic cardiovascular disease(ASCVD)is compromised by the concurrent elevation of proprotein convertase subtilisin/kexin type 9(PCSK9),a pivotal molecule that increases LDL-C levels.Aerobic exercise lowers PCSK9 levels,but the underlying mechanism remains unclear.Therefore,we investigated how aerobic exercise can ameliorate statin-induced increases in PCSK9 levels.Methods:Three-week-old male American Institute of Cancer Research(ICR)mice were fed a high-fat-cholesterol diet(HFD)for 12 weeks and then administered atorvastatin alone or atorvastatin combined with aerobic exercise(Statin+Ex).Moreover,a total of 165 participants with stable coronary heart disease(CHD)enrolled at the Inpatient and Outpatient Departments of the Second Xiangya Hospital of Central South University,China,from January 2018 to July 2020 were randomized into the Statin group(male/female=51/33)and Statin+Ex group(male/female=52/29).Patients in the Statin+Ex group underwent treadmill exercise of 45-60 min/day for 7 days.Results:Aerobic exercise effectively alleviated statin-induced PCSK9 upregulation in human patients with CHD and hypercholesterolemic ICR mice(all p<0.05).Mechanistically,our findings revealed that aerobic exercise induced elevated epoxyeicosatrienoic acids(EETs)plasma levels while concurrently reducing the activity of soluble epoxide hydrolase(sEH)(all p<0.05),an enzyme responsible for EETs degradation.Further,EETs significantly suppressed PCSK9 expression,subsequently reducing the LDL-C levels(all p<0.05);this effect was mediated via the activation of the forkhead box O3a-silent mating type information regulation 2 homolog 6(FoxO3a-Sirt6)axis,with no impact on the sterol regulatory element binding protein 2 and 3-hydroxy-3-methylglutaryl-CoA reductase(SREBP2-HMGCR)pathway.Conclusion:Our study sheds light on the paradigm of"Exercise is Medicine",providing evidence to support the use of statins combined with exercise in reducing LDL-C levels,and unveils potential avenues for clinical applications of sEH inhibitors,presenting novel prospects for therapeutic interventions in ASCVD.展开更多
BACKGROUND Type 2 diabetes mellitus(T2DM),a chronic metabolic disease with a high global incidence,has become a serious public health challenge.China has the largest number of T2DM patients worldwide,imposing a signif...BACKGROUND Type 2 diabetes mellitus(T2DM),a chronic metabolic disease with a high global incidence,has become a serious public health challenge.China has the largest number of T2DM patients worldwide,imposing a significant economic burden on the healthcare system.T2DM is closely associated with insulin resistance,impaired pancreatic B cell function,and disordered glucose and lipid metabolism,which can lead to various complications,reducing patients'quality of life and increasing the risk of disability and death.Thus,finding effective preventive and intervention measures is crucial.Exercise therapy,a key part of diabetes management,has gained attention in recent years,with many studies indicating its benefits for blood glucose control and other aspects in diabetic patients.AIM To assess the effectiveness of combined resistance and aerobic exercise interventions on blood glucose control and metabolic indicators in patients with T2DM and to explore their application in diabetes management.METHODS Systematic searches were conducted using PubMed,EMBASE,Cochrane Library,and Chinese databases for relevant randomized controlled trials(RCTs).The inclusion criteria were participants aged≥18 years with T2DM and the intervention involved combined resistance and aerobic exercise for≥8 weeks.The primary outcome indicators were fasting blood glucose,low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),triglycerides(TG),glycated hemoglobin A1c(HbA1c),and total cholesterol(TC)levels.Data analysis was performed using RevMan software,and the interventional effects were assessed using weighted mean differences or standardized mean differences(SMD).RESULTS Six RCTs meeting the inclusion criteria were included,with a total sample size of 366 participants.The meta-analysis results showed that combined resistance and aerobic exercise significantly improved several metabolic indicators in patients with T2DM.Specific results were as follows:(1)For fasting blood glucose,combined exercise was more effective than aerobic exercise alone[SMD=1.22;95%confidence interval(95%CI):0.70,1.74;P<0.00001];(2)LDL-C levels were significantly reduced by the combined intervention(SMD=1.45;95%CI:1.18-1.72;P<0.00001);(3)The combined intervention significantly increased HDL-C levels(SMD=1.42;95%CI:0.98-1.87;P<0.00001);(4)The combined intervention significantly reduced TG levels(SMD=1.12;95%CI:0.85-1.39;P<0.00001;(5)No statistically significant difference was observed in HbA1c between the combined and the aerobic exercise group(SMD=-0.03;95%CI:-1.09 to 1.04;P<0.00001);and(6)The combined exercise intervention group significantly reduced TC levels(SMD=2.66;95%CI:1.93-3.38;P<0.00001).The subgroup analysis results suggest that the effect of exercise interventions may be influenced by various factors,including the patient's age,baseline blood glucose levels,and exercise intensity.CONCLUSION Combined resistance and aerobic exercise intervention significantly improved fasting blood glucose,LDL-C,HDL-C,TG,and TC levels in patients with T2DM,especially in terms of blood glucose control and cardiovascular risk,demonstrating better outcomes than aerobic exercise alone.展开更多
Acute lung injury(ALI)/acute respiratory distress syndrome(ARDS)is a severe clinical disorder characterized by widespread inflammation,diffuse alveolar damage,and pulmonary edema,often leading to respiratory failure a...Acute lung injury(ALI)/acute respiratory distress syndrome(ARDS)is a severe clinical disorder characterized by widespread inflammation,diffuse alveolar damage,and pulmonary edema,often leading to respiratory failure and death.Despite significant advances in clinical care,ALI/ARDS remains the leading cause of death among intensive care unit patients.Sepsis is the primary risk factor for the development of ALI/ARDS,as excessive inflammatory responses contribute to organ injury and high mortality in critically ill patients.展开更多
Objective:To explore the effect of cognitive intervention combined with aerobic limb rehabilitation exercise on neurological and limb functions in hemiplegic patients with hypertensive intracerebral hemorrhage.Methods...Objective:To explore the effect of cognitive intervention combined with aerobic limb rehabilitation exercise on neurological and limb functions in hemiplegic patients with hypertensive intracerebral hemorrhage.Methods:A prospective study was conducted with eighty-six hemiplegic patients with hypertensive intracerebral hemorrhage admitted to Taikang Xianlin Drum Tower Hospital from October 2021 to October 2023.The patients were randomly divided into a study group(43 cases)and a control group(43 cases).After 6 months of intervention,neurological function,motor and limb function,psychological state,and quality of life scores were compared between the two groups to assess the effects of the intervention.Results:After the intervention,neurological function indexes in the study group,including S100B protein,myelin basic protein and National Institutes of Health Stroke Scale score,were significantly lower than those in the control group(all P<0.05).In contrast,brain-derived neurotrophic factor and Mini-Mental State Examination scores were significantly higher in the study group(all P<0.05).Additionally,motor function,Barthel score,Fugl-Meyer score,and muscle strength(grades V and IV)were significantly higher in the study group compared to the control group(all P<0.05).Psychological resilience,including scores for toughness,optimism,and strength,as well as the total resilience score,were significantly higher in the study group(all P<0.05).Moreover,the Generic Quality of Life Inventory score was also significantly higher in the study group(P<0.05).Conclusion:Cognitive intervention combined with aerobic limb rehabilitation exercises can alleviate neurological damage in hemiplegic patients with hypertensive intracerebral hemorrhage,enhance muscle strength,promote limb and motor function recovery,and improve psychological state and quality of life.展开更多
Salmonella grows better under aerobic conditions as a facultative anaerobic foodborne pathogenic bacteria.The oxygen-scavenging activity of Lactococcus lactis in the intestinal tract is a promising strategy for preven...Salmonella grows better under aerobic conditions as a facultative anaerobic foodborne pathogenic bacteria.The oxygen-scavenging activity of Lactococcus lactis in the intestinal tract is a promising strategy for preventing Salmonella infection.In this study,the aerobic respiration requirement and preventive mechanism of L.lactis subsp.lactis KLDS 4.0325 in murine models infected by Salmonella enterica subsp.enterica serovar Typhimurium(S.Typhimurium)SL1344 were investigated.Results indicate that L.lactis KLDS 4.0325 is capable of aerobic respiratory metabolism in the host intestine when exogenous heme exists,and decrease intestinal oxygen concentration,which in turn trigger autophagy of intestinal cells to reduce S.Typhimurium load,improve gut microbiota composition,alleviate intestinal barrier injury and inflammation response.These results suggest that aerobic respiration L.lactis KLDS 4.0325 can prevent S.Typhimurium infection in a new way in which by restoring intestinal cell hypoxia,maintaining immune balance and regulating intestinal flora.展开更多
It is well known that appropriate aerobic exercise can effectively alleviate fatty liver and enhance brain function.The concept of multi-organ crosstalk coordinating disease progression has become the current research...It is well known that appropriate aerobic exercise can effectively alleviate fatty liver and enhance brain function.The concept of multi-organ crosstalk coordinating disease progression has become the current research hot topic.However,there remains an urgent need to elucidate its specific mechanisms.This study aimed to explore the impact of a high-fat diet(HFD)on liver health and cognitive function,and to further uncover the regulatory effect of aerobic exercise by liver-specific activating transcription factor 3(Atf3)knockout(ATF3cKO)mice in a“liver-brain”axis mode.The 5-week-old C57BL/6 and ATF3cKO mice were fed with HFD for 32 weeks,and sequentially subjected to aerobic exercise intervention at the 20th week for another 12 consecutive weeks.Meanwhile,C57BL/6 mice were provided with a normal diet as the control group.The functional parameters of liver and brain of all mice were assessed.Cognitive capacity of all mice was assessed by the Morris water maze(MWM).Inflammatory factors in the serum and brain of mice were quantified using enzyme-linked immunosorbent assay(ELISA),and the expression of inflammasomes was detected by immunohistochemistry(IHC).Additionally,the activation of nuclear factor-κB(NF-κB)and phosphoinositide 3-kinase(PI3K)signal pathways was analyzed by Western blotting.In this study,HFD impaired hepatic and brain functions,while aerobic exercise and liver-specific Atf3 knockout suppressed inflammatory factors in the peripheral circulation through hepatoprotective mechanisms,thereby attenuating cerebral inflammation and preserving neurological integrity,as well as mitigating HFD-induced cognitive decline.展开更多
Anxiety disorders,characterized by persistent apprehension,somatic symptoms and fatigue,are leading causes of disability worldwide.The burgeoning therapeutic potential of aerobic exercise has gained prominence as a le...Anxiety disorders,characterized by persistent apprehension,somatic symptoms and fatigue,are leading causes of disability worldwide.The burgeoning therapeutic potential of aerobic exercise has gained prominence as a leading non-pharmacological strategy,with evidence supporting its effectiveness in alleviating anxiety across diverse conditions.This review synthesizes current research to clarify the molecular mechanisms through which aerobic exercise ameliorates anxiety in terms of the effects of exercise on the hypothalamic–pituitary–adrenal(HPA)axis,the hepatic-brain axis and epigenetics;electroencephalographic alterations;inflammatory pathways;the balance between oxidative and nitrogenous stress;various substances,such as brain-derived neurotrophic factor(BDNF),atrial natriuretic peptide(ANP),and opioid peptides;and the 5-HT2C receptor and cannabinoid receptor type-1(CB1R),among others,reflecting the positive modulatory effects of aerobic exercise on anxiety.As a non-pharmacological intervention,aerobic exercise has been demonstrated to be useful in a variety of medical applications and has considerable potential for ameliorating symptoms of anxiety.展开更多
With the rapid advancement of robotics and Artificial Intelligence(AI),aerobics training companion robots now support eco-friendly fitness by reducing reliance on nonrenewable energy.This study presents a solar-powere...With the rapid advancement of robotics and Artificial Intelligence(AI),aerobics training companion robots now support eco-friendly fitness by reducing reliance on nonrenewable energy.This study presents a solar-powered aerobics training robot featuring an adaptive energy management system designed for sustainability and efficiency.The robot integrates machine vision with an enhanced Dynamic Cheetah Optimizer and Bayesian Neural Network(DynCO-BNN)to enable precise exercise monitoring and real-time feedback.Solar tracking technology ensures optimal energy absorption,while a microcontroller-based regulator manages power distribution and robotic movement.Dual-battery switching ensures uninterrupted operation,aided by light and I/V sensors for energy optimization.Using the INSIGHT-LME IMU dataset,which includes motion data from 76 individuals performing Local Muscular Endurance(LME)exercises,the system detects activities,counts repetitions,and recognizes human movements.To minimize energy use during data processing,Min-Max normalization and two-dimensional Discrete Fourier Transform(2D-DFT)are applied,boosting computational efficiency.The robot accurately identifies upper and lower limb movements,delivering effective exercise guidance.The DynCO-BNN model achieved a high tracking accuracy of 96.8%.Results confirm improved solar utilization,ecological sustainability,and reduced dependence on fossil fuels—positioning the robot as a smart,energy-efficient solution for next-generation fitness technology.展开更多
In this study,the effect of number of stages and bioreactor type on the removal performance of a sequential anaerobic-aerobic process employing activated sludge for the treatment of a simulated textile dyeing wastewat...In this study,the effect of number of stages and bioreactor type on the removal performance of a sequential anaerobic-aerobic process employing activated sludge for the treatment of a simulated textile dyeing wastewater containing three commercial reactive azo dyes was considered.Two stage processes performed better than one stage ones,both in terms of overall organic and color removal,as well as the higher contribution of anaerobic stage to the overall removal performance,thereby making them a more energy efficient option.The employment of a moving bed sequencing batch biofilm reactor,which uses both suspended and attached biomass,for the implementation of the anaerobic stage of the process,was compared with a sequencing batch reactor that only employs suspended biomass.The results showed that,although there was no meaningful difference in biomass concentration between the two bioreactors,the latter reactor had better performance in terms of chemical oxygen demand(COD)removal efficiency and rate and color removal rate.Further exploratory tests revealed a difference between the roles of suspended and attached bacterial populations,with the former yielding better color removal whilst the latter had better COD removal performance.The sequential anaerobic–aerobic process,employing an aerobic membrane bioreactor in the aerobic stage resulted in COD and color removal of 77.1±7.9%and 79.9±1.5%,respectively.The incomplete COD and color removal was attributed to the presence of soluble microbial products in the effluent and the autoxidation of dye reduction metabolites,respectively.Also,aerobic partial mineralization of the dye reduction metabolites,was experimentally observed.展开更多
Research Background: Stroke rehabilitation is essential for improving patient outcomes, with a focus on restoring functionality, strength, and mobility. Aerobic (TAE) and anaerobic (TAN) training have demonstrated var...Research Background: Stroke rehabilitation is essential for improving patient outcomes, with a focus on restoring functionality, strength, and mobility. Aerobic (TAE) and anaerobic (TAN) training have demonstrated varying impacts on post-stroke recovery. Objective: This systematic review and meta-analysis aimed to compare the effects of TAE and TAN on post-stroke rehabilitation outcomes, including functionality, walking improvement, strength, balance, and cardiorespiratory capacity. Methods: A comprehensive literature search was conducted in the PubMed and PEDro databases, covering studies from January 2014 to May 2024. Randomized controlled trials (RCTs) evaluating the impact of TAE and TAN on the specified outcomes were included. The review adhered to PRISMA guidelines, and independent reviewers extracted relevant data on participant characteristics, interventions, and outcomes. The methodological quality of the included studies was assessed using the PEDro scale, and the risk of bias was analyzed. Results: Data synthesis revealed that TAN was more effective in improving performance in the 10-Meter Walk Test (10MWT) and the Berg Balance Scale (BBS), while TAE demonstrated superior results in the Timed Up and Go (TUG) test and the Barthel Activities of Daily Living Index (Barthel ADL). Both training modalities showed significant improvements in the 6-Minute Walk Test (6MWT) for cardiorespiratory capacity, with TAN exhibiting a slightly higher mean difference. Surprisingly, strength gains, assessed by Maximal Isometric Strength (MaxIS), were higher in the TAE group. Conclusions: Both TAE and TAN contribute to post-stroke recovery, with TAN excelling in walking and balance improvements, and TAE showing advantages in functional mobility and strength. The findings support personalized rehabilitation strategies that incorporate both aerobic and anaerobic training to optimize patient outcomes.展开更多
Aerobic anoxygenic phototrophic bacteria(AAPB)are significant bacterial groups in aquatic ecosystems,known for their rapid growth and photoheterotrophic characteristics.However,the distribution and ecological assembly...Aerobic anoxygenic phototrophic bacteria(AAPB)are significant bacterial groups in aquatic ecosystems,known for their rapid growth and photoheterotrophic characteristics.However,the distribution and ecological assembly process of AAPB in low irradiation freshwater basins remain unclear,warranting further investigation.In this study,we present the diversity,abundance,spatial variations,ecological process,and community interaction of AAPB in sediment of Three Gorges Reservoir(TGR)under low irradiation.Our findings demonstrate the dominant genera of AAPB community that exist in the TGR area also are appeared in different waters,with some regional preference.Moreover,the concentration of pufM gene,an indicator for AAPB,maintains a consistently high numerical level ranging from(2.21±0.44)×10^(4)to(9.98±0.30)×10^(7)gene copies/g.Although solar irradiation is suggested as the major factor affecting AAPB,it remains unclear whether and how AAPB differ between regions due to varying solar irradiation levels.Our results show spatial differences between total bacteria and AAPB communities,with significant differences observed only in AAPB.Geographical and environmental factor contributed less than 10%to the spatial difference of community,with sediment type and environmental factors being the key factors influencing microbial community structure.The stochastic process plays a dominant role in the aggregation and replacement of AAPB communities,among which the most contribution is dispersal limitation.For AAPB network,Yoonia and Gemmobacter are the hubs for modules.Those results valuable insights into the AAPB communities in TGR with low irradiation.展开更多
BACKGROUND Pulmonary hypertension is a serious complication in the treatment of maintenance hemodialysis patients,which seriously affects the quality of life of patients and threatens their life safety.Prevention,trea...BACKGROUND Pulmonary hypertension is a serious complication in the treatment of maintenance hemodialysis patients,which seriously affects the quality of life of patients and threatens their life safety.Prevention,treatment and improvement of pulmonary hypertension are of great significance to improve the quality of life of patients.AIM To investigate the intervention and control of pedal-powered bicycle in maintaining quality of life and pulmonary hypertension in hemodialysis patients.METHODS 73 patients with maintenance hemadialysis combined with pulmonary arterial hypertension at a hemodialysis center in a certain hospital from May 2021 to May 2022 are selected.Patients are divided into two groups,37 cases in the control group(group C)and 36 cases in the intervention group(group I).Patients are divided into two groups,group C is treated with oral administration of betaglandin sodium combined with routine nursing care.Based on group C,group I conducts power cycling exercises.RESULTS After treatment,group I patients had higher muscle strength,36-Item Short Form Health Survey scores,and Kidney Disease Targets Areas scores;The 6-minute walk distance test index level was higher and the Borg score was lower;The group I had lower systolic blood pressure,greater vital capacity,higher positive emotion,lower systolic pulmonary artery pressure index level,higher arterial partial oxygen pressure level,lower pulmonary vascular resistance index level,and higher blood oxygen saturation level[158.91±11.89 vs 152.56±12.81,1795.01±603.18 vs 1907.20±574.15,24.00(22.00,29.00)vs 24.00(22.00,28.00),P<0.001].CONCLUSION Aerobic exercise combined with Western medicine treatment can effectively improve patients'pulmonary hypertension,alleviate their negative emotions,and enable them to achieve a higher level of quality of life.展开更多
Sulfide oxidation under aerobic conditions can produce active oxygen for the transformation of organic pollutants in aquatic environments.However,the catalytic performance of transition metal-supported carbon material...Sulfide oxidation under aerobic conditions can produce active oxygen for the transformation of organic pollutants in aquatic environments.However,the catalytic performance of transition metal-supported carbon material on this process is poor understood.This study found that Co-loaded carbon nanotubes(CNTs)was able to realize the efficient aerobic transformation of antibiotic ciprofloxacin(CIP)by sulfide,with the pseudo-first order reaction rate constant improved from 0.013 h^(-1)without catalyst to 0.44–0.71 h^(-1)with 100 mg/L Co-loaded CNTs.Singlet oxygen(^(1)O_(2))was the main active specie playing key roles in the process of CIP aerobic transformation with presence of Co-loaded CNTs.Mechanism studies indicated that the excellent electron transfer ability of Co-loaded CNTs might play an important role to promote the electron transfer and facilitate the formation of intermediate H_(2)O_(2)and^(1)O_(2).Additionally,the Co-loaded CNTs/sulfide system effectively reduced the acute toxicity of organic pollutant,and Co-loaded CNTs showed remarkable cycling stability and negligible leaching.This study gives a better understanding for the Co-loaded CNTs mediated aerobic antibiotics transformation by sulfide,and provide a reference for the application of Co-loaded carbon materials on organics aerobic transformation by sulfide.展开更多
The screening and identification of attachment genes is important to exploring the formation mechanism of biofilms at the gene level.It is helpful to the development of key culture technologies for aerobic granular sl...The screening and identification of attachment genes is important to exploring the formation mechanism of biofilms at the gene level.It is helpful to the development of key culture technologies for aerobic granular sludge(AGS).In this study,genome-wide sequencing and gene editing were employed for the first time to investigate the effects and functions of attachment genes in AGS.With the help of whole-genome analysis,ten attachment genes were screened from thirteen genes,and the efficiency of gene screening was greatly improved.Then,two attachment genes were selected as examples to further confirm the gene functions by constructing gene-knockout recombinant mutants of Stenotrophomonas maltophilia;when the two attachment genes were knocked out,the attachment potential was reduced by 50.67%and 43.93%,respectively.The results provide a new theoretical principle and efficient method for the development of AGS from the perspective of attachment genes.展开更多
Background:Polymethoxylatedflavones(PMFs)are compounds present in citrus peels and other Rutaceae plants,which exhibit diverse biological activities,including robust antitumor and antioxidant effects.However,the mechan...Background:Polymethoxylatedflavones(PMFs)are compounds present in citrus peels and other Rutaceae plants,which exhibit diverse biological activities,including robust antitumor and antioxidant effects.However,the mechanism of PMFs in reversing drug resistance to colon cancer remains unknown.In the present study,we aimed to investigate the potential connection between the aerobic glycolysis-ROS-autophagy signaling axis and the reversal of PTX resistance in colon cancer by PMFs.Methods:MTT Cell viability assay and colony formation assay were used to investigate the effect of PMFs combined with PTX in reversing HCT8/T cell resistance ex vivo;the mRNA and protein levels of the target were detected by SDS-PAGE(sodium dodecyl sulfate-polyacrylamide gel electrophoresis),quantitative real-timefluorescence polymerase chain reaction(qRT-PCR)and Western blot protein immunoblotting(WB);An HCT8/T cell xenograft model was established to investigate the MDR reversal activity of PMFs in vivo;The extracellular acidification rate(ECAR)and the oxygen consumption rate(OCR)were detected to assess the cellular oxygen consumption rate and glycolytic process.Results:HCT8/T cells demonstrated significant resistance to PTX,up-regulating the expression levels of ABCB1 mRNA,P-gp,LC3-I,and LC3-II protein,and increasing intracellular reactive oxygen species(ROS)content.PMFs mainly contain two active ingredients,nobiletin,and tangeretin,which were able to reverse drug resistance in HCT8/T cells in a concentration-dependent manner.PMFs exhibited high tolerance in the HCT8/T nude mouse model while increasing the sensitivity of PTX-resistant cells and suppressing tumor growth significantly.PMFs combined with PTX reduced extracellular acidification rate(ECAR)and oxygen consumption rate(OCR)in HCT8/T cells.Additionally,PMFs reduced intracellular ROS content,down-regulated the expression levels of autophagy-related proteins LC3-I,LC3-II,Beclin1,and ATG7,and significantly reduced the number of autophagosomes in HCT8/T cells.Conclusions:The present study demonstrated that PMFs could potentially reverse PTX resistance in colon cancer by regulating the aerobic glycolysis-ROS-autophagy signaling axis,which indicated that PMFs would be potential potentiators for future chemotherapeutic agents in colon cancer.展开更多
基金funded by the National Natural Science Foundation of China(81871854,72374014)the National Key R&D Program of China(2020YFC2008804)+1 种基金the Shanghai Jiao Tong University Young Talent Cultivation Program in Liberal Arts(2024QN041)the Shanghai Jiao Tong University School of Medicine:Nursing Development Program(SJTUHLXK2024).
文摘Background Evidence on the effects of different exercise interventions on cognitive function is insufficient.Aims To evaluate the feasibility and effects of remotely supervised aerobic exercise(AE)and resistance exercise(RE)interventions in older adults with mild cognitive impairment(MCI).Methods This study is a 6-month pilot three-arm randomised controlled trial.Eligible participants(n=108)were recruited and randomised to the AE group,RE group or control(CON)group with a 1:1:1 ratio.Interventions were delivered at home with remote supervision.We evaluated participants’global cognition,memory,executive function,attention,physical activity levels,physical performance and muscle strength of limbs at baseline,3 months(T1)and 6 months(T2)after randomisation.A linear mixed-effects model was adopted for data analyses after controlling for covariates.Tukey’s method was used for adjusting for multiple comparisons.Sensitivity analyses were performed after excluding individuals with low compliance rates.Results 15(13.89%)participants dropped out.The median compliance rates in the AE group and RE group were 67.31%and 93.27%,respectively.After adjusting for covariates,the scores of the Alzheimer’s Disease Assessment Scale-Cognitive subscale in the AE group decreased by 2.04(95%confidence interval(CI)−3.41 to−0.67,t=−2.94,p=0.004)and 1.53(95%CI−2.88 to−0.17,t=−2.22,p=0.028)points more than those in the CON group at T1 and T2,respectively.The effects of AE were still significant at T1(estimate=−1.70,95%CI−3.20 to−0.21,t=−2.69,p=0.021),but lost statistical significance at T2 after adjusting for multiple comparisons.As for executive function,the Stroop time interference in the RE group decreased by 11.76 s(95%CI−21.62 to−1.90,t=−2.81,p=0.015)more than that in the AE group at T2 after Tukey’s adjustment.No other significant effects on cognitive functions were found.Conclusions Both remotely supervised AE and RE programmes are feasible in older adults with MCI.AE has positive effects on global cognition,and RE improves executive function.
文摘A visible-light-enabled method for the synthesis ofα-azidoketones has been developed via oxo-azidation of alkenyl silanes with trimethylsilylazide and molecular oxygen under mild conditions.The reaction could be carried out in gram scale.Various radical sources,including trifluoromethyl radical,thiocyanate radical,bromide radical,chlorine radical could partici-pate effectively instead of azide radical in the reaction.
文摘[Objectives]To synthesize evidence on HIIT versus moderate-intensity continuous training(MICT)or routine rehabilitation in stroke survivors.[Methods]We systematically searched 8 databases(PubMed,EMBASE,CENTRAL,Web of Science,SPORTSDiscus,PsycINFO,SCOPUS,CINAHL)up to May 2025.Seventeen randomized controlled trials(RCTs;total n=1142)met inclusion criteria:adults with stroke,device-based HIIT(≥70%HRR/VO 2peak),and outcomes assessing VO 2peak,6-min walk distance(6MWD),or Berg Balance Scale(BBS).Methodological quality was evaluated using the PEDro scale.Pooled effect sizes(Hedges'g)were calculated via random-effects models,with heterogeneity quantified by I^(2).[Results]HIIT significantly improved peak oxygen uptake(VO 2peak)versus controls(g=0.59,95%CI:0.44-0.75,p<0.001;I^(2)=16.29%).Low heterogeneity and symmetrical funnel plots supported robustness.HIIT also enhanced walking endurance(6MWD:g=0.32,95%CI:0.16-0.48,p<0.01;I^(2)=30%).In contrast,no significant benefit was observed for balance function(BBS:g=0.07,95%CI:-0.13-0.26,p=0.50;I^(2)=0%).[Conclusions]HIIT is a safe and highly effective intervention for enhancing aerobic capacity and walking function post-stroke.Its benefits are maximized at higher intensities and longer durations but do not extend to balance improvement.Integrating HIIT into stroke rehabilitation protocols is strongly recommended to promote functional independence.
基金supported by funding provided to BJG from the Natural Sciences and Engineering Research Council of Canada(NSERCgrant no.402635).
文摘Objectives:Aerobic exercise training can increase skeletal muscle mitochondrial content.Supine exercise training with legs above the heart potentially augments these increases.However,the impact of supine exercise training on mitochondrial biogenesis and cardiovascular adaptations remains unclear.Methods:In this single-centred,randomized,parallel arm trial,19 recreationally active individuals underwent seven sessions of either supine with legs up(SUP;n=9,6 females)or upright with legs down(UP;n=10,7 females)aerobic training on a recumbent bike at 71±7%and 71±2%of peak work rate(WRpeak),respectively.The study aimed to test the effects of training with decreased muscle oxygenation on indices of muscle mitochondrial remodelling.Secondary outcomes included exercise performance,muscle oxygenation,and cardiovascular responses.Results:Secondary outcomes revealed significant interaction effects for time to fatigue(TTF)and WRpeak in the SUP group during supine testing,suggesting enhanced exercise tolerance and performance.No between group interaction effects were observed for upright testing.No clear effects on mitochondrial biogenesis were observed based on expression of mitochondrial protein subunits and transcriptional regulators.Acutely,HRpeak was lower during the SUP Test compared to the UP Test.No central cardiovascular adaptations were observed following training.Conclusions:Our exploratory analyses showed that supine aerobic training more effectively improves supine exercise tolerance and performance compared with upright training,despite no differences in measured proteins related to mitochondrial biogenesis.Further research is needed to elucidate the mechanisms underlying these postural-specific training effects.Registration:clinicaltrials.gov:NCT04151095.
基金supported by the Key Technologies Research and Development Program of Guangzhou (No.2023B03J1284)Guangdong Yuehai Water Investment Co.,Ltd. (No.CC80-QT01-2020-0010).
文摘This work established a quantitative method to access the shear stability of aerobic granular sludge(AGS)and validated its feasibility by using the mature AGS from a pilot-scale(50 tons/day)membrane bioreactor(MBR)for treating real municipal wastewater.The results showed that the changing rate( S)of the peak area(S)of granule size distribution(GSD)exhibited an exponential relationship(R^(2)≥0.76)with the shear time(y=a−b·c^(x)),which was a suitable indicative index to reflect the shear stability of different AGS samples.The limiting granule size(LGS)was defined and proposed to characterize the equilibrium size for AGS after being sheared for a period of time,whose value in terms of Dv50 showed high correlation(R^(2)=0.92)with the parameter a.The free Ca^(2+)(28.44-34.21 mg/L)in the influent specifically interacted with polysaccharides(PS)in the granule’s extracellular polymeric substance(EPS)as a nucleation site,thereby inducing the formation of Ca precipitation to enhance its Young’s modulus,while Ca^(2+) primarily interacted with PS in soluble metabolic product(SMP)during the initial granulation process.Furthermore,the Young’s modulus significantly affected the parameter a related to shear stability(R^(2)=0.99).Since the parameter a was more closely related(R^(2)=1.00)to S than that of the parameter b or c,the excellent correlation(R^(2)=0.99)between the parameter a and the wet density further verified the feasibility of this method.
基金supported by the National Natural Science Foundation of China(No.81871858 and No.82172550).
文摘Background:Statins are the cornerstone of low-density lipoprotein cholesterol(LDL-C)-lowering therapy;however,the therapeutic efficacy of statins in countering atherosclerotic cardiovascular disease(ASCVD)is compromised by the concurrent elevation of proprotein convertase subtilisin/kexin type 9(PCSK9),a pivotal molecule that increases LDL-C levels.Aerobic exercise lowers PCSK9 levels,but the underlying mechanism remains unclear.Therefore,we investigated how aerobic exercise can ameliorate statin-induced increases in PCSK9 levels.Methods:Three-week-old male American Institute of Cancer Research(ICR)mice were fed a high-fat-cholesterol diet(HFD)for 12 weeks and then administered atorvastatin alone or atorvastatin combined with aerobic exercise(Statin+Ex).Moreover,a total of 165 participants with stable coronary heart disease(CHD)enrolled at the Inpatient and Outpatient Departments of the Second Xiangya Hospital of Central South University,China,from January 2018 to July 2020 were randomized into the Statin group(male/female=51/33)and Statin+Ex group(male/female=52/29).Patients in the Statin+Ex group underwent treadmill exercise of 45-60 min/day for 7 days.Results:Aerobic exercise effectively alleviated statin-induced PCSK9 upregulation in human patients with CHD and hypercholesterolemic ICR mice(all p<0.05).Mechanistically,our findings revealed that aerobic exercise induced elevated epoxyeicosatrienoic acids(EETs)plasma levels while concurrently reducing the activity of soluble epoxide hydrolase(sEH)(all p<0.05),an enzyme responsible for EETs degradation.Further,EETs significantly suppressed PCSK9 expression,subsequently reducing the LDL-C levels(all p<0.05);this effect was mediated via the activation of the forkhead box O3a-silent mating type information regulation 2 homolog 6(FoxO3a-Sirt6)axis,with no impact on the sterol regulatory element binding protein 2 and 3-hydroxy-3-methylglutaryl-CoA reductase(SREBP2-HMGCR)pathway.Conclusion:Our study sheds light on the paradigm of"Exercise is Medicine",providing evidence to support the use of statins combined with exercise in reducing LDL-C levels,and unveils potential avenues for clinical applications of sEH inhibitors,presenting novel prospects for therapeutic interventions in ASCVD.
基金Supported by Research Project of Zhejiang Provincial Science and Technology Plan for Traditional Chinese Medicine,No.2024ZL753and Research Project of Zhejiang Provincial Medical and Health Science and Technology Plan,No.2024KY1406.
文摘BACKGROUND Type 2 diabetes mellitus(T2DM),a chronic metabolic disease with a high global incidence,has become a serious public health challenge.China has the largest number of T2DM patients worldwide,imposing a significant economic burden on the healthcare system.T2DM is closely associated with insulin resistance,impaired pancreatic B cell function,and disordered glucose and lipid metabolism,which can lead to various complications,reducing patients'quality of life and increasing the risk of disability and death.Thus,finding effective preventive and intervention measures is crucial.Exercise therapy,a key part of diabetes management,has gained attention in recent years,with many studies indicating its benefits for blood glucose control and other aspects in diabetic patients.AIM To assess the effectiveness of combined resistance and aerobic exercise interventions on blood glucose control and metabolic indicators in patients with T2DM and to explore their application in diabetes management.METHODS Systematic searches were conducted using PubMed,EMBASE,Cochrane Library,and Chinese databases for relevant randomized controlled trials(RCTs).The inclusion criteria were participants aged≥18 years with T2DM and the intervention involved combined resistance and aerobic exercise for≥8 weeks.The primary outcome indicators were fasting blood glucose,low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),triglycerides(TG),glycated hemoglobin A1c(HbA1c),and total cholesterol(TC)levels.Data analysis was performed using RevMan software,and the interventional effects were assessed using weighted mean differences or standardized mean differences(SMD).RESULTS Six RCTs meeting the inclusion criteria were included,with a total sample size of 366 participants.The meta-analysis results showed that combined resistance and aerobic exercise significantly improved several metabolic indicators in patients with T2DM.Specific results were as follows:(1)For fasting blood glucose,combined exercise was more effective than aerobic exercise alone[SMD=1.22;95%confidence interval(95%CI):0.70,1.74;P<0.00001];(2)LDL-C levels were significantly reduced by the combined intervention(SMD=1.45;95%CI:1.18-1.72;P<0.00001);(3)The combined intervention significantly increased HDL-C levels(SMD=1.42;95%CI:0.98-1.87;P<0.00001);(4)The combined intervention significantly reduced TG levels(SMD=1.12;95%CI:0.85-1.39;P<0.00001;(5)No statistically significant difference was observed in HbA1c between the combined and the aerobic exercise group(SMD=-0.03;95%CI:-1.09 to 1.04;P<0.00001);and(6)The combined exercise intervention group significantly reduced TC levels(SMD=2.66;95%CI:1.93-3.38;P<0.00001).The subgroup analysis results suggest that the effect of exercise interventions may be influenced by various factors,including the patient's age,baseline blood glucose levels,and exercise intensity.CONCLUSION Combined resistance and aerobic exercise intervention significantly improved fasting blood glucose,LDL-C,HDL-C,TG,and TC levels in patients with T2DM,especially in terms of blood glucose control and cardiovascular risk,demonstrating better outcomes than aerobic exercise alone.
基金supported by National Natural Science Foundation of China(82471792,82270004,81870061,82202382,31770945)Beijing Municipal Natural Science Foundation(7242135)Zhejiang Provincial Natural Science Foundation(LY20H010003).
文摘Acute lung injury(ALI)/acute respiratory distress syndrome(ARDS)is a severe clinical disorder characterized by widespread inflammation,diffuse alveolar damage,and pulmonary edema,often leading to respiratory failure and death.Despite significant advances in clinical care,ALI/ARDS remains the leading cause of death among intensive care unit patients.Sepsis is the primary risk factor for the development of ALI/ARDS,as excessive inflammatory responses contribute to organ injury and high mortality in critically ill patients.
文摘Objective:To explore the effect of cognitive intervention combined with aerobic limb rehabilitation exercise on neurological and limb functions in hemiplegic patients with hypertensive intracerebral hemorrhage.Methods:A prospective study was conducted with eighty-six hemiplegic patients with hypertensive intracerebral hemorrhage admitted to Taikang Xianlin Drum Tower Hospital from October 2021 to October 2023.The patients were randomly divided into a study group(43 cases)and a control group(43 cases).After 6 months of intervention,neurological function,motor and limb function,psychological state,and quality of life scores were compared between the two groups to assess the effects of the intervention.Results:After the intervention,neurological function indexes in the study group,including S100B protein,myelin basic protein and National Institutes of Health Stroke Scale score,were significantly lower than those in the control group(all P<0.05).In contrast,brain-derived neurotrophic factor and Mini-Mental State Examination scores were significantly higher in the study group(all P<0.05).Additionally,motor function,Barthel score,Fugl-Meyer score,and muscle strength(grades V and IV)were significantly higher in the study group compared to the control group(all P<0.05).Psychological resilience,including scores for toughness,optimism,and strength,as well as the total resilience score,were significantly higher in the study group(all P<0.05).Moreover,the Generic Quality of Life Inventory score was also significantly higher in the study group(P<0.05).Conclusion:Cognitive intervention combined with aerobic limb rehabilitation exercises can alleviate neurological damage in hemiplegic patients with hypertensive intracerebral hemorrhage,enhance muscle strength,promote limb and motor function recovery,and improve psychological state and quality of life.
基金supported by the National Natural Science Foundation of China(32072190 and 32101929)Academic Backbone Plan of Northeast Agricultural University(20XG12)。
文摘Salmonella grows better under aerobic conditions as a facultative anaerobic foodborne pathogenic bacteria.The oxygen-scavenging activity of Lactococcus lactis in the intestinal tract is a promising strategy for preventing Salmonella infection.In this study,the aerobic respiration requirement and preventive mechanism of L.lactis subsp.lactis KLDS 4.0325 in murine models infected by Salmonella enterica subsp.enterica serovar Typhimurium(S.Typhimurium)SL1344 were investigated.Results indicate that L.lactis KLDS 4.0325 is capable of aerobic respiratory metabolism in the host intestine when exogenous heme exists,and decrease intestinal oxygen concentration,which in turn trigger autophagy of intestinal cells to reduce S.Typhimurium load,improve gut microbiota composition,alleviate intestinal barrier injury and inflammation response.These results suggest that aerobic respiration L.lactis KLDS 4.0325 can prevent S.Typhimurium infection in a new way in which by restoring intestinal cell hypoxia,maintaining immune balance and regulating intestinal flora.
基金supported by the National Natural Science Foundation of China(32471186 and 31771318)the 14th Five-Year-Plan Advantageous and Characteristic Disciplines(Groups)of Colleges and Universities in Hubei Province for Exercise and Brain Science+1 种基金as well as the Leading Talent Program and Innovative Start-up Foundation from Wuhan Sports University to Ning Chensupported by the Hubei Natural Science Foundation(2022CFB929)and China Postdoctoral Science Foundation(2023M732727)to Tong Wu.
文摘It is well known that appropriate aerobic exercise can effectively alleviate fatty liver and enhance brain function.The concept of multi-organ crosstalk coordinating disease progression has become the current research hot topic.However,there remains an urgent need to elucidate its specific mechanisms.This study aimed to explore the impact of a high-fat diet(HFD)on liver health and cognitive function,and to further uncover the regulatory effect of aerobic exercise by liver-specific activating transcription factor 3(Atf3)knockout(ATF3cKO)mice in a“liver-brain”axis mode.The 5-week-old C57BL/6 and ATF3cKO mice were fed with HFD for 32 weeks,and sequentially subjected to aerobic exercise intervention at the 20th week for another 12 consecutive weeks.Meanwhile,C57BL/6 mice were provided with a normal diet as the control group.The functional parameters of liver and brain of all mice were assessed.Cognitive capacity of all mice was assessed by the Morris water maze(MWM).Inflammatory factors in the serum and brain of mice were quantified using enzyme-linked immunosorbent assay(ELISA),and the expression of inflammasomes was detected by immunohistochemistry(IHC).Additionally,the activation of nuclear factor-κB(NF-κB)and phosphoinositide 3-kinase(PI3K)signal pathways was analyzed by Western blotting.In this study,HFD impaired hepatic and brain functions,while aerobic exercise and liver-specific Atf3 knockout suppressed inflammatory factors in the peripheral circulation through hepatoprotective mechanisms,thereby attenuating cerebral inflammation and preserving neurological integrity,as well as mitigating HFD-induced cognitive decline.
基金supported by the National Natural Science Foundation of China(No.82405550 and No.82374584).
文摘Anxiety disorders,characterized by persistent apprehension,somatic symptoms and fatigue,are leading causes of disability worldwide.The burgeoning therapeutic potential of aerobic exercise has gained prominence as a leading non-pharmacological strategy,with evidence supporting its effectiveness in alleviating anxiety across diverse conditions.This review synthesizes current research to clarify the molecular mechanisms through which aerobic exercise ameliorates anxiety in terms of the effects of exercise on the hypothalamic–pituitary–adrenal(HPA)axis,the hepatic-brain axis and epigenetics;electroencephalographic alterations;inflammatory pathways;the balance between oxidative and nitrogenous stress;various substances,such as brain-derived neurotrophic factor(BDNF),atrial natriuretic peptide(ANP),and opioid peptides;and the 5-HT2C receptor and cannabinoid receptor type-1(CB1R),among others,reflecting the positive modulatory effects of aerobic exercise on anxiety.As a non-pharmacological intervention,aerobic exercise has been demonstrated to be useful in a variety of medical applications and has considerable potential for ameliorating symptoms of anxiety.
文摘With the rapid advancement of robotics and Artificial Intelligence(AI),aerobics training companion robots now support eco-friendly fitness by reducing reliance on nonrenewable energy.This study presents a solar-powered aerobics training robot featuring an adaptive energy management system designed for sustainability and efficiency.The robot integrates machine vision with an enhanced Dynamic Cheetah Optimizer and Bayesian Neural Network(DynCO-BNN)to enable precise exercise monitoring and real-time feedback.Solar tracking technology ensures optimal energy absorption,while a microcontroller-based regulator manages power distribution and robotic movement.Dual-battery switching ensures uninterrupted operation,aided by light and I/V sensors for energy optimization.Using the INSIGHT-LME IMU dataset,which includes motion data from 76 individuals performing Local Muscular Endurance(LME)exercises,the system detects activities,counts repetitions,and recognizes human movements.To minimize energy use during data processing,Min-Max normalization and two-dimensional Discrete Fourier Transform(2D-DFT)are applied,boosting computational efficiency.The robot accurately identifies upper and lower limb movements,delivering effective exercise guidance.The DynCO-BNN model achieved a high tracking accuracy of 96.8%.Results confirm improved solar utilization,ecological sustainability,and reduced dependence on fossil fuels—positioning the robot as a smart,energy-efficient solution for next-generation fitness technology.
基金supported by Takmiliran textile dyeing factory(272219601)Materials and Energy Research Center(MERC)(99392003).
文摘In this study,the effect of number of stages and bioreactor type on the removal performance of a sequential anaerobic-aerobic process employing activated sludge for the treatment of a simulated textile dyeing wastewater containing three commercial reactive azo dyes was considered.Two stage processes performed better than one stage ones,both in terms of overall organic and color removal,as well as the higher contribution of anaerobic stage to the overall removal performance,thereby making them a more energy efficient option.The employment of a moving bed sequencing batch biofilm reactor,which uses both suspended and attached biomass,for the implementation of the anaerobic stage of the process,was compared with a sequencing batch reactor that only employs suspended biomass.The results showed that,although there was no meaningful difference in biomass concentration between the two bioreactors,the latter reactor had better performance in terms of chemical oxygen demand(COD)removal efficiency and rate and color removal rate.Further exploratory tests revealed a difference between the roles of suspended and attached bacterial populations,with the former yielding better color removal whilst the latter had better COD removal performance.The sequential anaerobic–aerobic process,employing an aerobic membrane bioreactor in the aerobic stage resulted in COD and color removal of 77.1±7.9%and 79.9±1.5%,respectively.The incomplete COD and color removal was attributed to the presence of soluble microbial products in the effluent and the autoxidation of dye reduction metabolites,respectively.Also,aerobic partial mineralization of the dye reduction metabolites,was experimentally observed.
文摘Research Background: Stroke rehabilitation is essential for improving patient outcomes, with a focus on restoring functionality, strength, and mobility. Aerobic (TAE) and anaerobic (TAN) training have demonstrated varying impacts on post-stroke recovery. Objective: This systematic review and meta-analysis aimed to compare the effects of TAE and TAN on post-stroke rehabilitation outcomes, including functionality, walking improvement, strength, balance, and cardiorespiratory capacity. Methods: A comprehensive literature search was conducted in the PubMed and PEDro databases, covering studies from January 2014 to May 2024. Randomized controlled trials (RCTs) evaluating the impact of TAE and TAN on the specified outcomes were included. The review adhered to PRISMA guidelines, and independent reviewers extracted relevant data on participant characteristics, interventions, and outcomes. The methodological quality of the included studies was assessed using the PEDro scale, and the risk of bias was analyzed. Results: Data synthesis revealed that TAN was more effective in improving performance in the 10-Meter Walk Test (10MWT) and the Berg Balance Scale (BBS), while TAE demonstrated superior results in the Timed Up and Go (TUG) test and the Barthel Activities of Daily Living Index (Barthel ADL). Both training modalities showed significant improvements in the 6-Minute Walk Test (6MWT) for cardiorespiratory capacity, with TAN exhibiting a slightly higher mean difference. Surprisingly, strength gains, assessed by Maximal Isometric Strength (MaxIS), were higher in the TAE group. Conclusions: Both TAE and TAN contribute to post-stroke recovery, with TAN excelling in walking and balance improvements, and TAE showing advantages in functional mobility and strength. The findings support personalized rehabilitation strategies that incorporate both aerobic and anaerobic training to optimize patient outcomes.
基金supported by the National Natural Science Foundation of China(Nos.52070179,52270190,52131003)the Scientific research instrument development project of Chinese Academy of Sciences(No.YJKYYQ2020004)the Outstanding Scientist of Chongqing Talent Program(No.CQYC20210101288)。
文摘Aerobic anoxygenic phototrophic bacteria(AAPB)are significant bacterial groups in aquatic ecosystems,known for their rapid growth and photoheterotrophic characteristics.However,the distribution and ecological assembly process of AAPB in low irradiation freshwater basins remain unclear,warranting further investigation.In this study,we present the diversity,abundance,spatial variations,ecological process,and community interaction of AAPB in sediment of Three Gorges Reservoir(TGR)under low irradiation.Our findings demonstrate the dominant genera of AAPB community that exist in the TGR area also are appeared in different waters,with some regional preference.Moreover,the concentration of pufM gene,an indicator for AAPB,maintains a consistently high numerical level ranging from(2.21±0.44)×10^(4)to(9.98±0.30)×10^(7)gene copies/g.Although solar irradiation is suggested as the major factor affecting AAPB,it remains unclear whether and how AAPB differ between regions due to varying solar irradiation levels.Our results show spatial differences between total bacteria and AAPB communities,with significant differences observed only in AAPB.Geographical and environmental factor contributed less than 10%to the spatial difference of community,with sediment type and environmental factors being the key factors influencing microbial community structure.The stochastic process plays a dominant role in the aggregation and replacement of AAPB communities,among which the most contribution is dispersal limitation.For AAPB network,Yoonia and Gemmobacter are the hubs for modules.Those results valuable insights into the AAPB communities in TGR with low irradiation.
基金Zhejiang Province Medicine and Health Science and Technology Programme,No.2019RC092 and No.2020KY394the Public Welfare Project of Zhejiang Provincial Department of Science and Technology,No.LGF19H170003.
文摘BACKGROUND Pulmonary hypertension is a serious complication in the treatment of maintenance hemodialysis patients,which seriously affects the quality of life of patients and threatens their life safety.Prevention,treatment and improvement of pulmonary hypertension are of great significance to improve the quality of life of patients.AIM To investigate the intervention and control of pedal-powered bicycle in maintaining quality of life and pulmonary hypertension in hemodialysis patients.METHODS 73 patients with maintenance hemadialysis combined with pulmonary arterial hypertension at a hemodialysis center in a certain hospital from May 2021 to May 2022 are selected.Patients are divided into two groups,37 cases in the control group(group C)and 36 cases in the intervention group(group I).Patients are divided into two groups,group C is treated with oral administration of betaglandin sodium combined with routine nursing care.Based on group C,group I conducts power cycling exercises.RESULTS After treatment,group I patients had higher muscle strength,36-Item Short Form Health Survey scores,and Kidney Disease Targets Areas scores;The 6-minute walk distance test index level was higher and the Borg score was lower;The group I had lower systolic blood pressure,greater vital capacity,higher positive emotion,lower systolic pulmonary artery pressure index level,higher arterial partial oxygen pressure level,lower pulmonary vascular resistance index level,and higher blood oxygen saturation level[158.91±11.89 vs 152.56±12.81,1795.01±603.18 vs 1907.20±574.15,24.00(22.00,29.00)vs 24.00(22.00,28.00),P<0.001].CONCLUSION Aerobic exercise combined with Western medicine treatment can effectively improve patients'pulmonary hypertension,alleviate their negative emotions,and enable them to achieve a higher level of quality of life.
基金the National Natural Science Foundation of China(Nos.52200186,U19A20108,52025101 and 52070025)China Postdoctoral Science Foundation(No.2021M693720)Chongqing Municipal Education Commission(No.KJCX2020001)for financially supporting this study。
文摘Sulfide oxidation under aerobic conditions can produce active oxygen for the transformation of organic pollutants in aquatic environments.However,the catalytic performance of transition metal-supported carbon material on this process is poor understood.This study found that Co-loaded carbon nanotubes(CNTs)was able to realize the efficient aerobic transformation of antibiotic ciprofloxacin(CIP)by sulfide,with the pseudo-first order reaction rate constant improved from 0.013 h^(-1)without catalyst to 0.44–0.71 h^(-1)with 100 mg/L Co-loaded CNTs.Singlet oxygen(^(1)O_(2))was the main active specie playing key roles in the process of CIP aerobic transformation with presence of Co-loaded CNTs.Mechanism studies indicated that the excellent electron transfer ability of Co-loaded CNTs might play an important role to promote the electron transfer and facilitate the formation of intermediate H_(2)O_(2)and^(1)O_(2).Additionally,the Co-loaded CNTs/sulfide system effectively reduced the acute toxicity of organic pollutant,and Co-loaded CNTs showed remarkable cycling stability and negligible leaching.This study gives a better understanding for the Co-loaded CNTs mediated aerobic antibiotics transformation by sulfide,and provide a reference for the application of Co-loaded carbon materials on organics aerobic transformation by sulfide.
基金supported by the National Natural Science Foundation of China(No.51578069)Beijing Science and Technology Commission Project(No.Z171100000717012)。
文摘The screening and identification of attachment genes is important to exploring the formation mechanism of biofilms at the gene level.It is helpful to the development of key culture technologies for aerobic granular sludge(AGS).In this study,genome-wide sequencing and gene editing were employed for the first time to investigate the effects and functions of attachment genes in AGS.With the help of whole-genome analysis,ten attachment genes were screened from thirteen genes,and the efficiency of gene screening was greatly improved.Then,two attachment genes were selected as examples to further confirm the gene functions by constructing gene-knockout recombinant mutants of Stenotrophomonas maltophilia;when the two attachment genes were knocked out,the attachment potential was reduced by 50.67%and 43.93%,respectively.The results provide a new theoretical principle and efficient method for the development of AGS from the perspective of attachment genes.
基金supported by National Natural Science Foundation of China(82104446)Guangdong Basic and Applied Basic Research Foundation(2023A1515011961)+3 种基金Guangdong Province Characteristic Innovation Project of Universities(2022KTSCX100)Guangzhou University(College)-(High Level University/Deng feng Hospital)Basic and Applied Basic Research Project(2023A03J0397)Guangdong Medical Science and Technology Research Foundation(A2023460)Plan on Enhancing Scientific Research in GMU(2024SRP117).
文摘Background:Polymethoxylatedflavones(PMFs)are compounds present in citrus peels and other Rutaceae plants,which exhibit diverse biological activities,including robust antitumor and antioxidant effects.However,the mechanism of PMFs in reversing drug resistance to colon cancer remains unknown.In the present study,we aimed to investigate the potential connection between the aerobic glycolysis-ROS-autophagy signaling axis and the reversal of PTX resistance in colon cancer by PMFs.Methods:MTT Cell viability assay and colony formation assay were used to investigate the effect of PMFs combined with PTX in reversing HCT8/T cell resistance ex vivo;the mRNA and protein levels of the target were detected by SDS-PAGE(sodium dodecyl sulfate-polyacrylamide gel electrophoresis),quantitative real-timefluorescence polymerase chain reaction(qRT-PCR)and Western blot protein immunoblotting(WB);An HCT8/T cell xenograft model was established to investigate the MDR reversal activity of PMFs in vivo;The extracellular acidification rate(ECAR)and the oxygen consumption rate(OCR)were detected to assess the cellular oxygen consumption rate and glycolytic process.Results:HCT8/T cells demonstrated significant resistance to PTX,up-regulating the expression levels of ABCB1 mRNA,P-gp,LC3-I,and LC3-II protein,and increasing intracellular reactive oxygen species(ROS)content.PMFs mainly contain two active ingredients,nobiletin,and tangeretin,which were able to reverse drug resistance in HCT8/T cells in a concentration-dependent manner.PMFs exhibited high tolerance in the HCT8/T nude mouse model while increasing the sensitivity of PTX-resistant cells and suppressing tumor growth significantly.PMFs combined with PTX reduced extracellular acidification rate(ECAR)and oxygen consumption rate(OCR)in HCT8/T cells.Additionally,PMFs reduced intracellular ROS content,down-regulated the expression levels of autophagy-related proteins LC3-I,LC3-II,Beclin1,and ATG7,and significantly reduced the number of autophagosomes in HCT8/T cells.Conclusions:The present study demonstrated that PMFs could potentially reverse PTX resistance in colon cancer by regulating the aerobic glycolysis-ROS-autophagy signaling axis,which indicated that PMFs would be potential potentiators for future chemotherapeutic agents in colon cancer.
