目的探讨α2A肾上腺素能受体(ADRA2A)-1291C>G和胆囊收缩素受体1(CCK1R)-779T>C的多态性与肠易激综合征(irritable bowel syndrome,IBS)的关系。方法利用PubMed、Embase、ISI科学网和万方数据库的在线网站,在不限制语言的情况下,...目的探讨α2A肾上腺素能受体(ADRA2A)-1291C>G和胆囊收缩素受体1(CCK1R)-779T>C的多态性与肠易激综合征(irritable bowel syndrome,IBS)的关系。方法利用PubMed、Embase、ISI科学网和万方数据库的在线网站,在不限制语言的情况下,检索1996年6月至2019年5月的相关文献。结果ADRA2A-1291C>G的C等位基因和CCK1R-779T>C的T等位基因与IBS发病风险的增加密切相关。亚组分析表明,ADRA2A-1291C>G增加了显性模型中IBS-C(CC vs GG+CG:OR=1.38,95%CI:1.00~1.90)和IBS-D(CC vs GG+CG:OR=1.50,95%CI:1.06~2.14)的风险。结论ADRA2A-1291C>G的C等位基因与CCK1R-779T>C的T等位基因密切相关,提示IBS的危险性增加。为了验证其关联性,还需要更大的样本进行进一步的研究。展开更多
Objective The alpha 2A-adrenergic receptor gene （ADRA2A） polymorphism in individuals antiplatelet response to sympathetic stimulation. The aim of this study was to investigate ADRA2A variants on platelet reactivity ...Objective The alpha 2A-adrenergic receptor gene （ADRA2A） polymorphism in individuals antiplatelet response to sympathetic stimulation. The aim of this study was to investigate ADRA2A variants on platelet reactivity in Chinese patients on dual antiplatelet therapy undergoing percutaneous coronary intervention （PCI）. modifies the the effect of （DAPT） after Methods From March 2011 to March 2013, 1,024 patients were enrolled in this prospective, single-center, observational study in China. Four single nucleotide polymorphisms （SNPs） of ADRA2A gene （rs11195419, rs3750625, rs13306146, and rs553668） and CYP2C19^＊2 were detected by ligase detection reaction （LDR）, and adenosine diphosphate （ADP） inhibition was detected by thromboelastography （TEG）. Results The minor allele frequencies of ADRA2A SNPs were common. Platelet ADP inhibition was significantly different among patients carrying rs11195419 （adjusted P = 0.022） and rs3750625 （adjusted P = 0.016）. The homozygous allele carriers had the lowest ADP inhibition. However, ADP inhibition was not significantly different in rs553668 and rs13306146. At the multivariate analysis, rs11195419 （P = 0.033）, rs3750625 （P = 0.020） and CYP2C19＂2 （P = 0.002） were independent predictors of ADP inhibition. Subgroups analysis based on sex showed rs11195419 （P = 0.003） and rs3750625 （P = 0.002） were significantly associated with ADP inhibition in males, but not in females. Conclusion ADRA2A genetic variations were associated with ADP-induced platelet aggregation during DAPT in Chinese patients undergoing PCI, and the effect was particularly more pronounced in males.展开更多
Background The ADRA2B gene insertion/deletion （I/D） polymorphism is associated with various cardiovascular and metabolic phenotypes. Large （C1） and small （C2） artery compliance, assessed by pulse wave analysis, ...Background The ADRA2B gene insertion/deletion （I/D） polymorphism is associated with various cardiovascular and metabolic phenotypes. Large （C1） and small （C2） artery compliance, assessed by pulse wave analysis, is considered as sensitive markers or risk factors for cardiovascular disease. Therefore whether the ADRA2B I/D polymorphism is associated with CI and C2 need to be investigated. Methods A total of 227 men and 243 women were enrolled in a Chinese family-based study. C1 and C2 were measured by pulse wave analysis. ADRA2B genotypes were determined by polymerase chain reaction. Statistical methods included generalized estimation equations and quantitative transmission disequilibrium test. Results The II （31.9%）, ID （46.8%） and DD （21.3%） genotype frequencies were in Hardy-Weinberg equilibrium （P = 0. 73 ）. The covariates selected by stepwise regression for C1 and C2 were age, systolic pressure and gender. The population based association analysis showed that C1 and C2 were not associated with ADRA2B genotype both before （C1 ： P =0. 28; C2： P =0. 27） and after （C1 ： P =0. 58; C2： P =0. 18） the adjustment. The family-based analyses of 128 informative offspring showed that transmission of the D-allele was not associated with C1 orC2, both before （CI： P=0.42; C2： P=0.85） and after （CI： P=0.31; C2： P= 0. 82） the adjustment. Conclusion The study do not support that the ADRA2B gene I/D polymorphism has a major gene effect on C1 or C2 in the Chinese population of current sample size.展开更多
文摘目的探讨α2A肾上腺素能受体(ADRA2A)-1291C>G和胆囊收缩素受体1(CCK1R)-779T>C的多态性与肠易激综合征(irritable bowel syndrome,IBS)的关系。方法利用PubMed、Embase、ISI科学网和万方数据库的在线网站,在不限制语言的情况下,检索1996年6月至2019年5月的相关文献。结果ADRA2A-1291C>G的C等位基因和CCK1R-779T>C的T等位基因与IBS发病风险的增加密切相关。亚组分析表明,ADRA2A-1291C>G增加了显性模型中IBS-C(CC vs GG+CG:OR=1.38,95%CI:1.00~1.90)和IBS-D(CC vs GG+CG:OR=1.50,95%CI:1.06~2.14)的风险。结论ADRA2A-1291C>G的C等位基因与CCK1R-779T>C的T等位基因密切相关,提示IBS的危险性增加。为了验证其关联性,还需要更大的样本进行进一步的研究。
基金supported by grant from the National Natural Science Foundation of China[81470486]
文摘Objective The alpha 2A-adrenergic receptor gene （ADRA2A） polymorphism in individuals antiplatelet response to sympathetic stimulation. The aim of this study was to investigate ADRA2A variants on platelet reactivity in Chinese patients on dual antiplatelet therapy undergoing percutaneous coronary intervention （PCI）. modifies the the effect of （DAPT） after Methods From March 2011 to March 2013, 1,024 patients were enrolled in this prospective, single-center, observational study in China. Four single nucleotide polymorphisms （SNPs） of ADRA2A gene （rs11195419, rs3750625, rs13306146, and rs553668） and CYP2C19^＊2 were detected by ligase detection reaction （LDR）, and adenosine diphosphate （ADP） inhibition was detected by thromboelastography （TEG）. Results The minor allele frequencies of ADRA2A SNPs were common. Platelet ADP inhibition was significantly different among patients carrying rs11195419 （adjusted P = 0.022） and rs3750625 （adjusted P = 0.016）. The homozygous allele carriers had the lowest ADP inhibition. However, ADP inhibition was not significantly different in rs553668 and rs13306146. At the multivariate analysis, rs11195419 （P = 0.033）, rs3750625 （P = 0.020） and CYP2C19＂2 （P = 0.002） were independent predictors of ADP inhibition. Subgroups analysis based on sex showed rs11195419 （P = 0.003） and rs3750625 （P = 0.002） were significantly associated with ADP inhibition in males, but not in females. Conclusion ADRA2A genetic variations were associated with ADP-induced platelet aggregation during DAPT in Chinese patients undergoing PCI, and the effect was particularly more pronounced in males.
基金The study was supported by a grant from the Natural ScienceFoundation of Jiangsu Province (No.BK2002029).
文摘Background The ADRA2B gene insertion/deletion （I/D） polymorphism is associated with various cardiovascular and metabolic phenotypes. Large （C1） and small （C2） artery compliance, assessed by pulse wave analysis, is considered as sensitive markers or risk factors for cardiovascular disease. Therefore whether the ADRA2B I/D polymorphism is associated with CI and C2 need to be investigated. Methods A total of 227 men and 243 women were enrolled in a Chinese family-based study. C1 and C2 were measured by pulse wave analysis. ADRA2B genotypes were determined by polymerase chain reaction. Statistical methods included generalized estimation equations and quantitative transmission disequilibrium test. Results The II （31.9%）, ID （46.8%） and DD （21.3%） genotype frequencies were in Hardy-Weinberg equilibrium （P = 0. 73 ）. The covariates selected by stepwise regression for C1 and C2 were age, systolic pressure and gender. The population based association analysis showed that C1 and C2 were not associated with ADRA2B genotype both before （C1 ： P =0. 28; C2： P =0. 27） and after （C1 ： P =0. 58; C2： P =0. 18） the adjustment. The family-based analyses of 128 informative offspring showed that transmission of the D-allele was not associated with C1 orC2, both before （CI： P=0.42; C2： P=0.85） and after （CI： P=0.31; C2： P= 0. 82） the adjustment. Conclusion The study do not support that the ADRA2B gene I/D polymorphism has a major gene effect on C1 or C2 in the Chinese population of current sample size.
