Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We rece...Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We recently identified zona pellucida(Zp) as the top differentially expressed protein between East Asian cyprinids that produce adhesive and semi-buoyant eggs,suggesting that Zp protein may play important roles in the regulation of egg type.In this work,we generated transgenic zebrafish in which oocyte-specific expression of zp genes from rare minnow(Gobiocypris rarus),an East Asian cyprinid laying adhesive eggs,was driven by a zebrafish zp3.2 gene promoter.We found that the transgenic eggs obtained partial adhesiveness and exhibited alteration in hydration and buoyancy.Abnormal metabolism of vitellogenin(VTG) may contribute to enhanced hydration and/or buoyancy.Our work shows that expression of the exogenous zp3a gene from an adhesiveegg producing fish is sufficient to induce changes in both egg adhesiveness and buoyancy in zebrafish,emphasizing the important role of zp genes in the regulation of spawning habits.Our results thus provide new insights into how endemic East Asian cyprinids may have adapted to the Yangtze river-lake system via changes in spawning habits.展开更多
Continuous monitoring of biosignals is essential for advancing early disease detection,personalized treatment,and health management.Flexible electronics,capable of accurately monitoring biosignals in daily life,have g...Continuous monitoring of biosignals is essential for advancing early disease detection,personalized treatment,and health management.Flexible electronics,capable of accurately monitoring biosignals in daily life,have garnered considerable attention due to their softness,conformability,and biocompatibility.However,several challenges remain,including imperfect skin-device interfaces,limited breathability,and insufficient mechanoelectrical stability.On-skin epidermal electronics,distinguished by their excellent conformability,breathability,and mechanoelectrical robustness,offer a promising solution for high-fidelity,long-term health monitoring.These devices can seamlessly integrate with the human body,leading to transformative advancements in future personalized healthcare.This review provides a systematic examination of recent advancements in on-skin epidermal electronics,with particular emphasis on critical aspects including material science,structural design,desired properties,and practical applications.We explore various materials,considering their properties and the corresponding structural designs developed to construct high-performance epidermal electronics.We then discuss different approaches for achieving the desired device properties necessary for long-term health monitoring,including adhesiveness,breathability,and mechanoelectrical stability.Additionally,we summarize the diverse applications of these devices in monitoring biophysical and physiological signals.Finally,we address the challenges facing these devices and outline future prospects,offering insights into the ongoing development of on-skin epidermal electronics for long-term health monitoring.展开更多
In view of the phenomenon that the adhesion strength between the surface of polyacrylonitrile-butadiene-styrene-polycarbonate(ABS-PC)copolymer and the electroless copper plating layer is relatively low.To solve the pr...In view of the phenomenon that the adhesion strength between the surface of polyacrylonitrile-butadiene-styrene-polycarbonate(ABS-PC)copolymer and the electroless copper plating layer is relatively low.To solve the problems of poor surface wettability and low surface roughness of the ABS-PC substrate,the N,N-dimethylformamide(DMF)-ethanol(C_(2)H_(5)OH)-water(H_(2)O)system was employed as the swelling system for the ABS-PC substrate.The effects of the DMF volume fraction in the swelling system and the swelling time on the swelling effect of ABS-PC at 35℃ were investigated.KMnO_(4)-H_(2)SO_(4)-H_(2)O system was used as the etching system for ABS-PC substrate under the conditions of the volume ratio of water to sulfuric acid of 1﹕2,with KMnO_(4) content of 30 g/L,etching temperature of 60℃,and etching time of 25 min.The results indicate that dense pores with uniform sized are formed on the surface of the ABS-PC substrate surface after swelling and etching treatments,accompanied by an increase in surface roughness when the swelling temperature is 35℃,the DMF volume fraction in the swelling system is 80%,and the swelling time is 5 min.Furthermore,the content of C element on the surface of the ABS-PC substrate decreased,while that of O element increased,and the surface hydrophilicity is enhanced,which is attributed to two hydrophilic groups,-C=O and-COOH,being generated on the ABS-PC substrate surface,significantly improving the wettability of the ABS-PC substrate surface.Under the combination effects of high surface roughness and strong surface hydrophilicity,the adhesion strength between the ABS-PC substrate surface and the electroless copper plating layer reached to 0.81 kN/m,meeting the adhesion strength requirement of 0.70 kN/m in the industrial production.展开更多
The net capturing method holds great potential for space debris removal due to its adaptability to the various target shapes and high fault tolerance.However,the capture mechanisms of current rope nets,which rely sole...The net capturing method holds great potential for space debris removal due to its adaptability to the various target shapes and high fault tolerance.However,the capture mechanisms of current rope nets,which rely solely on a passive wrap-ping mechanism,limit their capacity to capture objects within a specific size range and make it challenging to handle unexpected situations.Inspired by spider webs,which combine wrapping and adhering to capture prey of various sizes,we present a new type of net(envelope diameter:208.49 mm)for on-orbit capture.This net adopts a spiral symmetric structure similar to spider webs,incorporates electrostatic-microstructure hybrid adhesives,and increases the maximum contact area by 38.31%,allowing it to capture debris ranging from fragments smaller than the mesh size(envelope diam-eter:2.7 mm-4.4 mm)to larger objects(envelope diameter:270 mm),and effectively grasps flexible items(450 mm2),planar items(350 mm2)and three-dimensional items(160 mm3).Moreover,to validate the net's capability for wrapping and adhesion,simulations and experiments are demonstrated that this dual capture method can effectively handle various targets.展开更多
As a common electronic adhesive,ultraviolet(UV)curing polyurethane acrylate adhesive has both flexibility and wear resistance of polyurethane,excellent weather resistance and optical properties of acrylate.Despite the...As a common electronic adhesive,ultraviolet(UV)curing polyurethane acrylate adhesive has both flexibility and wear resistance of polyurethane,excellent weather resistance and optical properties of acrylate.Despite the extensive applications,it is still difficult to solve the problems caused by the shrinkage of adhesive.Here,a new type of photosensitive adhesive for bonding electronic components based on supramolecular interaction was designed and synthesized.The supramolecular interaction of cyclodextrin and adamantane moieties introduced into the adhesive polymer entitles the viscosity of the adhesive to rise rapidly during use,thereby preventing adhesive loss and dislocation of electronic components.UV light could further cure the adhesive and position the electronic components.The adhesive shrunk<2%when cured by UV light,so it can be used for electronic packaging and high-resolution,defect-free lithography.展开更多
As a specific spoilage organism of seafood under refrigerated temperature conditions,Shewanella spp.tend to form biofilms that exacerbate the occurrence of seafood spoilage.Biofilm-promoting factor A(BpfA)has been rep...As a specific spoilage organism of seafood under refrigerated temperature conditions,Shewanella spp.tend to form biofilms that exacerbate the occurrence of seafood spoilage.Biofilm-promoting factor A(BpfA)has been reported to promote the adhesion and biofilm formation of Shewanella spp.,but its role in adhesion and biofilm formation of S.putrefaciens under cold stress needs to be further investigated.To better comprehend the effect of BpfA on adhesion and biofilm formation of S.putrefaciens under cold stress(4℃),bacterial adhesion and biofilm phenotype of S.putrefaciens CN32 WT andΔbpfA at 4℃were analyzed and performed transcriptomics.The results showed that the deletion of bpfA had almost no effect on the growth of S.putrefaciens CN32 at 4℃,but weakened the unicellular adhesion capacity of S.putrefaciens CN32 and destabilized the stability of the multicellular adhesion layer.In addition,the biomass of the mature biofilm formed byΔbpfA was merely around 50%of that observed in the mature biofilm of S.putrefaciens CN32 WT,the average thickness and volume of the biofilm decreased by 18%and 27%,respectively,and the composition of the biofilm changed.Transcriptome analysis demonstrated that the deletion of bpfA led to differential expression of genes involved in metabolic pathways such as bacterial chemotaxis,two-component system,tyrosine metabolism,drug metabolism-other enzymes and biofilm formation-Vibrio cholerae,which in turn influenced bacterial adhesion and biofilm formation.Those results advance our acknowledgment of the character of BpfA on adhesion and biofilm formation of S.putrefaciens CN32,which contributes to understanding bacterial adhesion and the control of biofilm formation.展开更多
Silicone-based pressure-sensitive adhesives(Si-PSAs)are valued for their thermal stability,flexibility,and biocompatibility,but their weak bonding strength restricts high-performance use.Polyurethane-modified Si-PSAs ...Silicone-based pressure-sensitive adhesives(Si-PSAs)are valued for their thermal stability,flexibility,and biocompatibility,but their weak bonding strength restricts high-performance use.Polyurethane-modified Si-PSAs enhance adhesion,however diisocyanates remain essential.The raw materials of isocyanates are toxic,and their synthesis involves phosgene.To make up for those shortcomings,a series of poly(hydroxy urethane-siloxane)PSAs,named as PHUSi here,were synthesized through the ring-opening reaction of cyclic carbonate-functionalized polysiloxanes(PSi_(x)-VEC_(z))with various aliphatic diamines.The PSi_(x)-VEC_(z) precursors were prepared via the hydrosilylation of hydrogen-containing polysiloxanes(PSi_(x)-H_(y))with 4-vinyl-1,3-dioxolan-2-one(VEC).The chemical structures of PSi_(x)-H_(y),PSi_(x)-VEC_(z) and PHUSi were characterized,and bonding properties of PHUSi were systematically evaluated.The influence of architectures on adhesive performance was elucidated through comprehensive analyses,including rheology,crosslink density assessment,and so on.These studies revealed that the tailored design of PHUSi adhesives combine the advantages of traditional Si-PSAs with enhanced adhesion while eliminating isocyanate toxicity.The optimized PHUSi formulation achieved remarkable 180°peel strength(76.5 N/m on skin)and maximum probe tack force(1.61 N),enabling secure 24 h attachment of flexible sensors to skin.These properties make PHUSi particularly suitable for medical applications,as demonstrated by successful implementation in flexible electrocardiogram devices,offering a biocompatible,high-performance adhesive.展开更多
The effect of antibacterial adhesive on the biological corrosion resistance of mortar in seawater environment was studied by means of scanning electron microscope,thermogravimetric analysis,X-ray diffraction,Fourier t...The effect of antibacterial adhesive on the biological corrosion resistance of mortar in seawater environment was studied by means of scanning electron microscope,thermogravimetric analysis,X-ray diffraction,Fourier transform infrared spectroscopy,and ultra-depth microscope.The results show that the antibacterial adhesive can effectively inhibit the growth of sulfur-oxidizing bacteria in seawater,hinder their metabolism to produce biological sulfate,and reduce the formation of destructive product gypsum.The mineral composition and thermal analysis showed that the peak value of plaster diffraction peak and the mass loss of plaster dehydration in antibacterial adhesive group were significantly lower than those in blank group(without protective coating group).In addition,the electric flux of chloride ions(>400 C)in the blank group of mortar samples was higher than that in the antibacterial adhesive group(<200 C),indicating that the antibacterial adhesive can effectively reduce the permeability of chloride ions in mortar,and thus hinder the Cl-erosion in seawater.展开更多
Reuse/recycling of expensive components in next-generation optoelectronic systems(e.g.,flexible electronics and foldable displays)for sustainability purposes impose stringent requirements on optically clear adhesive(O...Reuse/recycling of expensive components in next-generation optoelectronic systems(e.g.,flexible electronics and foldable displays)for sustainability purposes impose stringent requirements on optically clear adhesive(OCA)with on-demand adhesion/deadhesion capability.Although some hot-melt OCAs have been developed,there still remain great challenges in balancing the mechanical property,adhesion/detachment and recyclability/reusability of the OCAs.Herein,we developed a debondable and robust polyurethane(PU)-based optically clear adhesive with both mechanical and chemical recyclability,overcoming the critical bottlenecks in sustainable advanced manufacturing.The ductile yet strong PU-based OCAs not only can adhere to various optically transparent glass and polymeric substrates(with a bonding strength high up to 5.0 MPa),but also exhibit deadhesion capability at high temperature owing to the thermally sensitive H-bonding interaction within the polymer network.The ondemand adhesion/deadhesion facilitates non-destructive detachment of functional assemblies and materials recycling/repair.The mechanical and chemical recyclability of PU-based OCAs can also effectively reduce the cost and address the endof-life issues.Moreover,the PU-based OCAs outperformed some commercial optical adhesives.In view of the straightforward synthesis,easy industrial scale-up and exceptional comprehensive properties,the PU-based OCAs will provide promising transformative solutions for advancing the circular economy and sustainability in next-generation optoelectronic devices.展开更多
The complex interactions and conflicting performance demands in multi-component composites pose significant challenges for achieving balanced multi-property optimization through conventional trial-and-error approaches...The complex interactions and conflicting performance demands in multi-component composites pose significant challenges for achieving balanced multi-property optimization through conventional trial-and-error approaches.Machine learning(ML)offers a promising solution,markedly improving materials discovery efficiency.However,the high dimensionality of feature spaces in such systems has long impeded effective ML-driven feature representation and inverse design.To overcome this,we present an Intelligent Screening System(ISS)framework to accelerate the discovery of optimal formulations balancing four key properties in 15-component PTFE-based copper-clad laminate composites(PTFE-CCLCs).ISS adopts modular descriptors based on the physical information of component volume fractions,thereby simplifying the feature representation.By leveraging the inverse prediction capability of ML models and constructing a performance-driven virtual candidate database,ISS significantly reduced the computational complexity associated with high-dimensional spaces.Experimental validation confirmed that ISSoptimized formulations exhibited superior synergy,notably resolving the trade-off between thermal conductivity and peel strength,and outperform many commercial counterparts.Despite limited data and inherent process variability,ISS achieved an average prediction accuracy of 76.5%,with thermal conductivity predictions exceeding 90%,demonstrating robust reliability.This work provides an innovative,efficient strategy for multifunctional optimization and accelerated discovery in ultra-complex composite systems,highlighting the integration of ML and advanced materials design.展开更多
Conducting hydrogels have garnered significant interest in the field of wearable electronics.However,simultaneously achieving high transparency,high conductivity,strong adhesion,and self-healing ability within a short...Conducting hydrogels have garnered significant interest in the field of wearable electronics.However,simultaneously achieving high transparency,high conductivity,strong adhesion,and self-healing ability within a short time remains a major challenge.In this study,a multifunctional mussel-inspired hydrogel was synthesized in only 5 min,with polydopamine(PDA)-polypyrrole(Ppy)-polyaniline(PANi)and poly(vinyl alcohol)(PVA)nanoparticles incorporated into the polyacrylamide(PAM)network.The resulting hydrogel exhibited high transparency(about 90% light transmission in the range of 400-800 nm),high conductivity((95.4±0.4)×10^(-4)S/cm),tensile strength(32.60±1.03 k Pa),strain at break(904.46%±11.50%),and adhesive strength(30-60 k Pa).It also demonstrated rapid self-healing properties(about 48% strength recovery within 1h at 50℃)and water-dependent shape memory behavior.As a wearable strain sensor,the hydrogel successfully detected finger flexion,wrist movements,facial expression changes,and breathing with high sensitivity and stability.The calculated gauge factor(GF)was 7.44±0.31,which is higher than that of many previously reported hydrogels.Compared with previous oyster-inspired or Ppy-based hydrogels,our system showed a much shorter synthesis time,higher transparency,and enhanced multifunctionality.These findings highlight the potential of the proposed hydrogel for next-generation flexible electronics,e-skin,and biomedical monitoring devices.展开更多
Carbon-fiber-reinforced plastics(CFRP)with improved mechanical properties based on modified epoxy binders were investigated in this study.By adding 15 parts by weight(p.b.w.)of copolymer of polysulfone with cardo phth...Carbon-fiber-reinforced plastics(CFRP)with improved mechanical properties based on modified epoxy binders were investigated in this study.By adding 15 parts by weight(p.b.w.)of copolymer of polysulfone with cardo phthalide group(PSFP-70C)to the epoxyanhydride binder,the flexural strength of the epoxy polymer was increased by 60%,the CFRP based on it by 57%,the flexural modulus of the epoxy polymer was increased by 83%,and the composite by 96%.The adhesion strength of the binder to carbon fiber reached a high level at 10 p.b.w.of thermoplastic modifier and increased by 65%compared to the unmodified binder.Scanning electron microscopy(SEM)was used to determine that in epoxyanhydride systems with a polysulfone content of 5–15 p.b.w.,the structure belongs to the"matrix dispersion"type and with a content of 20 p.b.w.to the"interpenetrating phase"type.A heterogeneous structure was also observed using dynamic mechanical analysis.展开更多
Objective:While cisplatin-based chemotherapy is pivotal for advanced bladder cancer,acquired resistance remains a major obstacle.This study investigates key molecular drivers of this resistance and potential reversal ...Objective:While cisplatin-based chemotherapy is pivotal for advanced bladder cancer,acquired resistance remains a major obstacle.This study investigates key molecular drivers of this resistance and potential reversal strategies.Methods:We established GC(Gemcitabine and Cisplatin)-resistant T24-R and UC3-R cell lines from T24 and UM-UC-3(UC3)cells.Transcriptomic and proteomic analyses identified differentially expressed molecules.Apoptosis and cell viability were assessed by flow cytometry and CCK-8(Cell Counting Kit-8)assays,while RT-qPCR(Reverse Transcription Quantitative Polymerase Chain Reaction)and Western blot analyzed gene and protein expression.Immunofluorescence evaluated FAK(Focal Adhesion Kinase)phosphorylation,and a xenograft mouse model validated the findings in vivo.Results:Integrated transcriptomic and proteomic analysis identified FN1(fibronectin)as a consistently upregulated top candidate in resistant cells(T24-R transcript log_(2)FC=2.8,protein log_(2)FC=0.9;UC3-R transcript log_(2)FC=3.7;all p<0.001).Knockdown of FN1 reduced chemoresistance(Resistance Index:5.2 in T24-R and 2.0 in UC3-R cells,p<0.001)and enhanced apoptosis(approximately 4.5-fold in T24-R and 7.5-fold in UC3-R,p<0.001).ITGB4(Integrin Subunit Beta 4)was upregulated in resistant cells(transcript log_(2)FC:4.2 in T24-R and 3.03 in UC3-R;protein log_(2)FC:0.67 in T24-R;all p<0.01).Critically,ITGB4 knockdown abolished the chemoresistance promoted by exogenous FN1,which was associated with increased FAK(Y397)phosphorylation.Conclusion:Our results demonstrate that the FN1-ITGB4 axis drives chemoresistance in bladder cancer via FAK signaling.Targeting this axis represents a promising strategy to overcome chemoresistance.展开更多
Driven by the trend of device miniaturization and high-density integration,the interaction between adjacent electrodes has become a critical factor affecting the interfacial reliability of thermoelectric(TE)structures...Driven by the trend of device miniaturization and high-density integration,the interaction between adjacent electrodes has become a critical factor affecting the interfacial reliability of thermoelectric(TE)structures.This study investigates the influence of adjoining electrode interactions on the interfacial response of a multi-electrode/TE substrate structure,including interfacial stresses and stress intensity factors at the electrode ends.To solve the corresponding boundary-value problem,the Fourier transforms are adopted to derive a governing integro-differential equation for the interfacial shear stress in multi-electrode systems,incorporating the TE effects as generalized forces on the right-hand side.The results show that both the interfacial tension and transverse stress in the electrodes are significantly affected by the presence of adjacent electrodes.The interaction between neighboring electrodes diminishes as their spacing increases or when an adhesive interlayer is introduced.Furthermore,the softer and thinner electrodes,the softer and thicker adhesive interlayer,and the smaller TE loads are found to be beneficial for improving the interfacial performance.These findings may contribute to the accurate measurement in surface sensors and layout design of multi-point health monitoring systems for TE structures.展开更多
The major aim of stroke therapy is to stimulate brain repair and improve behavioral recovery after cerebral ischemia.One option is to stimulate endogenous neurogenesis in the subventricular zone and direct the newly f...The major aim of stroke therapy is to stimulate brain repair and improve behavioral recovery after cerebral ischemia.One option is to stimulate endogenous neurogenesis in the subventricular zone and direct the newly formed neurons to the damaged area.However,only a small percentage of these neurons survive,and many do not reach the damaged area,possibly because the corpus callosum impedes the migration of subventricular zone-derived stem cells into the lesioned cortex.A second major obstacle to stem cell therapy is the strong inflammatory reaction induced by cerebral ischemia,whereby the associated phagocytic activity of brain macrophages removes both therapeutic cells and/or cell-based drug carriers.To address these issues,neurogenesis was electrically stimulated in the subventricular zone,followed by isolation of proliferating cells,including newly formed neurons,which were subsequently mixed with a nutritional hydrogel.This mixture was then transferred to the stroke cavity of day 14 post-stroke mice.We found that the performance of the treated animals improved in behavioral tests,including novel object,open field,hole board,grooming,and“time-to-feel”adhesive tape tests.Furthermore,immunostaining revealed that the stem cell marker nestin,the neuroepithelial marker Mash1,and the immature neuronal marker doublecortin-positive cells survived in the transplanted area for 2 weeks,possibly due to reduced phagocytic activity and supportive angiogenesis.These results clearly indicate that the transplantation of committed subventricular zone stem cells combined with a protective nutritional gel directly into the infarct cavity after the peak of stroke-induced neuroinflammation represents a feasible approach to improve neurorestoration after cerebral ischemia.展开更多
BACKGROUND Repeated application of the Pringle maneuver is a key obstacle to safe minimally invasive repeat liver resection(MISRLR).However,limited technical guidance is available.AIM To study the utility of newly dev...BACKGROUND Repeated application of the Pringle maneuver is a key obstacle to safe minimally invasive repeat liver resection(MISRLR).However,limited technical guidance is available.AIM To study the utility of newly developed Pringle taping method guided by liver surface in MISRLR.METHODS We retrospectively reviewed 72 cases of MISRLR performed by a single surgeon at two centers from August 2015 to July 2024.Beginning in October 2019,a liver surface-guided encirclement of hepatoduodenal ligament(LSEH)was used for repeat Pringle taping.Perioperative outcomes including Pringle taping success,operative time,blood loss,conversion rate,morbidity,and mortality were assessed.RESULTS Laparoscopic and robotic approaches were used in 63 patients and 9 patients,respectively.The median operative time,blood loss,and hospital stay were 331.5 minutes,70 mL,and 8 days,respectively.Open conversion occurred in two cases(2.8%)due to severe adhesions and right renal vein injury.Clavien-Dindo grade≥III complications occurred in 5.6%of cases with no mortality.Anti-adhesion barriers were used in 54 patients(75.0%).LSEH was attempted in 57 cases,improving Pringle taping success from 33.0%to 91.4%(P<0.001).LSEH succeeded in all patients with prior open liver resection(n=11).Among 6 patients in whom LSEH failed,3 patients(50.0%)had undergone a third liver resection,and 1 patient had a history of distal gastrectomy with choledochoduodenostomy.CONCLUSION The newly developed LSEH technique for Pringle taping in MISRLR was feasible,enhancing safety and reproducibility even in patients with a history of open liver resection.展开更多
Cellular immune response is a major barrier to xenotransplantation. Human tumor necrosis factor-α (hTNF-α) possesses cross-species activity and directly amplifies the immune rejection via the upregulation of adhesio...Cellular immune response is a major barrier to xenotransplantation. Human tumor necrosis factor-α (hTNF-α) possesses cross-species activity and directly amplifies the immune rejection via the upregulation of adhesion molecules on porcine endothelium. We investigated the role of protein tyrosine phosphorylation in the induction of expression of E-selectin and vascular cell adhesion molecule-1 (VCAM-1), and the augmentation of adhesion of human peripheral blood monocytes (PBMo) and natural killer cells (PBNK), after rhTNF-α-stimulation of porcine aortic endo-thelial cells (PAEC) in vitro. rhTNF-α-increased adhesiveness of PAEC for both PBMo and PBNK was dose-dependently reduced by pretreatment of PAEC with the selective protein tyrosine kinase (PTK) inhibitor genistein. The inhibitory effect occurred at the early time of PAEC activation triggered by rhTNF-α, and was completely reversible. PTK activity assay indicated that genistein also suppressed rhTNF-α stimulated activation of protein tyrosine展开更多
Objective:Endometrial tuberculosis,which commonly affects women of reproductive age,is a significant cause of intrauterine adhesions(IUA),potentially leading to hypomenorrhea,amenorrhea,and infertility.Hysteroscopic a...Objective:Endometrial tuberculosis,which commonly affects women of reproductive age,is a significant cause of intrauterine adhesions(IUA),potentially leading to hypomenorrhea,amenorrhea,and infertility.Hysteroscopic adhesiolysis is the primary treatment for IUA;however,studies specifically addressing its efficacy in tuberculosisinduced IUA remain scarce.This study aims to evaluate the therapeutic outcomes of hysteroscopic adhesiolysis for IUA caused by endometrial tuberculosis.Methods:This retrospective cohort study included patients diagnosed with tuberculosisinduced IUA who underwent hysteroscopic adhesiolysis at the Third Xiangya Hospital of Central South University between May 2014 and October 2022.Clinical data including age,medical history,adhesion severity,surgical treatment,and reproductive outcomes were analyzed.Results:Among 39 patients identified,2 were lost to follow-up.A total of 37 patients were included,with a follow-up duration ranging from 6 months to 9 years.Hypomenorrhea was reported in 24(64.9%)patients,secondary amenorrhea in 10(27.0%)patients,and normal menstruation in 3(8.1%)patients.Most patients presented with primary infertility(59.5%),and only 2(5.4%)had secondary infertility.The median American Fertility Society(AFS)score at initial assessment was 10(range,8−12);8(21.6%)patients had moderate IUA,and 29(78.4%)had severe IUA.A total of 86 surgical procedures were performed across 37 patients,with 27 patients undergoing 2 or more surgeries.Postoperatively,25(67.6%)patients achieved normalization of the uterine cavity,while 12(32.4%)still had a reduced cavity.Only 7(18.9%)patients had a grossly normal endometrium at the final surgery,all of whom had moderate adhesions at the initial procedure.Menstrual flow returned to normal in 12(32.4%)patients,while 25(67.6%)continued to experience hypomenorrhea.Of 29 patients who attempted in vitro fertilization and embryo transfer(IVF-ET),only 6(20.7%)conceived.Among these,4(13.8%)delivered at term via cesarean section;one case was complicated by postpartum hemorrhage due to uterine atony and another by placental adhesion.Conclusion:Endometrial tuberculosis can lead to severe IUA.Hysteroscopic adhesiolysis facilitates cavity restoration and improvement of menstrual conditions,but the overall reproductive outcomes remain suboptimal.展开更多
This review is composed of three main parts each of which is written by well-known top specialists that have been,in a way or other,also the main participants of the majority of the developments reported.Thus,after a ...This review is composed of three main parts each of which is written by well-known top specialists that have been,in a way or other,also the main participants of the majority of the developments reported.Thus,after a general part covering the grand lines and more in-depth views of more recent tannin,lignin,carbohydrate and soy bioadhesives,somemix of the other bio raw materials with soy protein and soy flour and some other differently sourced bioadhesives for wood,this review presents a more in-depth part on starch-based wood adhesives and a more indepth part covering plant protein-based adhesives.It must be kept in mind that the review is focused on completely or almost completely biosourced adhesives,the fashionable adhesives derived from mixes of biosourced materials with synthetic resins having been intentionally excluded.This choice was made as the latter constitute only an intermediate interval,possibly temporary if even for a somewhat long times,towards a final full bioeconomy of scale in this field.This review also focuses on more recent results,mainly obtained in the last 10–20 years,thus on adhesive formulations really innovative and sometimes even non-traditional.In all these fields there is still a lot of possibility of innovation for relevant formulation as this field is still in rapid growth.展开更多
Polymeric materials which can undergo controlled degradation and recycling are of great significance for a sustainable society.Although tremendous progress has been made in the degradation and recycling of both thermo...Polymeric materials which can undergo controlled degradation and recycling are of great significance for a sustainable society.Although tremendous progress has been made in the degradation and recycling of both thermoplastic and thermoset plastics,the development of high-performance degradable polymer adhesives is rare.Here,we have prepared high-performance nucleobase-containing thioctic acid-based supramolecular polymer adhesives through free radical polymerization.The specific hydrogen-bonding interactions between complementary nucleobases greatly improve the weak cohesion of the thioctic acid-based polymers and enhance the environmental stability of the thioctic acidbased polymers simultaneously.Degradation of the nucleobase-containing thioctic acid-based supramolecular polymers is achieved by the reduction of the disulfide backbone,and the cycle of degradation and repolymerization is further achieved via oxidative polymerization.The adhesion strength of the nucleobase-containing thioctic acid-based supramolecular polymers after two cycles of degradation and repolymerization still reaches as high as 4.7±0.3 MPa.This work provides an approach for the development of environmentally stable and high-performance degradable thioctic acid-based adhesives.展开更多
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB31000000)。
文摘Reproductive strategies and spawning habits play key roles in the evolution of endemic East Asian cyprinids.However,the molecular mechanisms underlying the regulation of spawning habits are not well understood.We recently identified zona pellucida(Zp) as the top differentially expressed protein between East Asian cyprinids that produce adhesive and semi-buoyant eggs,suggesting that Zp protein may play important roles in the regulation of egg type.In this work,we generated transgenic zebrafish in which oocyte-specific expression of zp genes from rare minnow(Gobiocypris rarus),an East Asian cyprinid laying adhesive eggs,was driven by a zebrafish zp3.2 gene promoter.We found that the transgenic eggs obtained partial adhesiveness and exhibited alteration in hydration and buoyancy.Abnormal metabolism of vitellogenin(VTG) may contribute to enhanced hydration and/or buoyancy.Our work shows that expression of the exogenous zp3a gene from an adhesiveegg producing fish is sufficient to induce changes in both egg adhesiveness and buoyancy in zebrafish,emphasizing the important role of zp genes in the regulation of spawning habits.Our results thus provide new insights into how endemic East Asian cyprinids may have adapted to the Yangtze river-lake system via changes in spawning habits.
基金supported by National Natural Science Foundation of China(Grant Nos.52025055,52375576,52350349)Key Research and Development Program of Shaanxi(Program No.2022GXLH-01-12)+2 种基金Joint Fund of Ministry of Education for Equipment Pre-research(No.8091B03012304)Aeronautical Science Foundation of China(No.2022004607001)the Fundamental Research Funds for the Central Universities(No.xtr072024031).
文摘Continuous monitoring of biosignals is essential for advancing early disease detection,personalized treatment,and health management.Flexible electronics,capable of accurately monitoring biosignals in daily life,have garnered considerable attention due to their softness,conformability,and biocompatibility.However,several challenges remain,including imperfect skin-device interfaces,limited breathability,and insufficient mechanoelectrical stability.On-skin epidermal electronics,distinguished by their excellent conformability,breathability,and mechanoelectrical robustness,offer a promising solution for high-fidelity,long-term health monitoring.These devices can seamlessly integrate with the human body,leading to transformative advancements in future personalized healthcare.This review provides a systematic examination of recent advancements in on-skin epidermal electronics,with particular emphasis on critical aspects including material science,structural design,desired properties,and practical applications.We explore various materials,considering their properties and the corresponding structural designs developed to construct high-performance epidermal electronics.We then discuss different approaches for achieving the desired device properties necessary for long-term health monitoring,including adhesiveness,breathability,and mechanoelectrical stability.Additionally,we summarize the diverse applications of these devices in monitoring biophysical and physiological signals.Finally,we address the challenges facing these devices and outline future prospects,offering insights into the ongoing development of on-skin epidermal electronics for long-term health monitoring.
文摘In view of the phenomenon that the adhesion strength between the surface of polyacrylonitrile-butadiene-styrene-polycarbonate(ABS-PC)copolymer and the electroless copper plating layer is relatively low.To solve the problems of poor surface wettability and low surface roughness of the ABS-PC substrate,the N,N-dimethylformamide(DMF)-ethanol(C_(2)H_(5)OH)-water(H_(2)O)system was employed as the swelling system for the ABS-PC substrate.The effects of the DMF volume fraction in the swelling system and the swelling time on the swelling effect of ABS-PC at 35℃ were investigated.KMnO_(4)-H_(2)SO_(4)-H_(2)O system was used as the etching system for ABS-PC substrate under the conditions of the volume ratio of water to sulfuric acid of 1﹕2,with KMnO_(4) content of 30 g/L,etching temperature of 60℃,and etching time of 25 min.The results indicate that dense pores with uniform sized are formed on the surface of the ABS-PC substrate surface after swelling and etching treatments,accompanied by an increase in surface roughness when the swelling temperature is 35℃,the DMF volume fraction in the swelling system is 80%,and the swelling time is 5 min.Furthermore,the content of C element on the surface of the ABS-PC substrate decreased,while that of O element increased,and the surface hydrophilicity is enhanced,which is attributed to two hydrophilic groups,-C=O and-COOH,being generated on the ABS-PC substrate surface,significantly improving the wettability of the ABS-PC substrate surface.Under the combination effects of high surface roughness and strong surface hydrophilicity,the adhesion strength between the ABS-PC substrate surface and the electroless copper plating layer reached to 0.81 kN/m,meeting the adhesion strength requirement of 0.70 kN/m in the industrial production.
基金the New Chongqing Innovative Young Talent Project under Grant 2024NSCQ-qncxX0468Dreams Foundation of Jianghuai Advance Technology Center under Grant 2023-ZM01Z007.
文摘The net capturing method holds great potential for space debris removal due to its adaptability to the various target shapes and high fault tolerance.However,the capture mechanisms of current rope nets,which rely solely on a passive wrap-ping mechanism,limit their capacity to capture objects within a specific size range and make it challenging to handle unexpected situations.Inspired by spider webs,which combine wrapping and adhering to capture prey of various sizes,we present a new type of net(envelope diameter:208.49 mm)for on-orbit capture.This net adopts a spiral symmetric structure similar to spider webs,incorporates electrostatic-microstructure hybrid adhesives,and increases the maximum contact area by 38.31%,allowing it to capture debris ranging from fragments smaller than the mesh size(envelope diam-eter:2.7 mm-4.4 mm)to larger objects(envelope diameter:270 mm),and effectively grasps flexible items(450 mm2),planar items(350 mm2)and three-dimensional items(160 mm3).Moreover,to validate the net's capability for wrapping and adhesion,simulations and experiments are demonstrated that this dual capture method can effectively handle various targets.
基金support from the National Natural Science Foundation of China(No.22308279)Guangdong Basic and Applied Basic Research Foundation(No.2021A1515110695)Natural Science Foundation of Chongqing(No.2023NSCQMSX2773).
文摘As a common electronic adhesive,ultraviolet(UV)curing polyurethane acrylate adhesive has both flexibility and wear resistance of polyurethane,excellent weather resistance and optical properties of acrylate.Despite the extensive applications,it is still difficult to solve the problems caused by the shrinkage of adhesive.Here,a new type of photosensitive adhesive for bonding electronic components based on supramolecular interaction was designed and synthesized.The supramolecular interaction of cyclodextrin and adamantane moieties introduced into the adhesive polymer entitles the viscosity of the adhesive to rise rapidly during use,thereby preventing adhesive loss and dislocation of electronic components.UV light could further cure the adhesive and position the electronic components.The adhesive shrunk<2%when cured by UV light,so it can be used for electronic packaging and high-resolution,defect-free lithography.
基金funded by the National Natural Science Foundation of China(32472401).
文摘As a specific spoilage organism of seafood under refrigerated temperature conditions,Shewanella spp.tend to form biofilms that exacerbate the occurrence of seafood spoilage.Biofilm-promoting factor A(BpfA)has been reported to promote the adhesion and biofilm formation of Shewanella spp.,but its role in adhesion and biofilm formation of S.putrefaciens under cold stress needs to be further investigated.To better comprehend the effect of BpfA on adhesion and biofilm formation of S.putrefaciens under cold stress(4℃),bacterial adhesion and biofilm phenotype of S.putrefaciens CN32 WT andΔbpfA at 4℃were analyzed and performed transcriptomics.The results showed that the deletion of bpfA had almost no effect on the growth of S.putrefaciens CN32 at 4℃,but weakened the unicellular adhesion capacity of S.putrefaciens CN32 and destabilized the stability of the multicellular adhesion layer.In addition,the biomass of the mature biofilm formed byΔbpfA was merely around 50%of that observed in the mature biofilm of S.putrefaciens CN32 WT,the average thickness and volume of the biofilm decreased by 18%and 27%,respectively,and the composition of the biofilm changed.Transcriptome analysis demonstrated that the deletion of bpfA led to differential expression of genes involved in metabolic pathways such as bacterial chemotaxis,two-component system,tyrosine metabolism,drug metabolism-other enzymes and biofilm formation-Vibrio cholerae,which in turn influenced bacterial adhesion and biofilm formation.Those results advance our acknowledgment of the character of BpfA on adhesion and biofilm formation of S.putrefaciens CN32,which contributes to understanding bacterial adhesion and the control of biofilm formation.
基金financially supported by the Shandong Provincial Natural Science Foundation,China(Nos.ZR2022MB051 and ZR2021MB112)Postdoctoral Science Foundation of China(No.2022M712343)+2 种基金Jinan City University Integration Development Strategy Project(No.JNSX2024030)Key Laboratory of Special Functional Aggregates of the Ministry of Education,Shandong University(No.JJT-2023-02)Shandong SD-Link New Material Technology Co.,LTD.
文摘Silicone-based pressure-sensitive adhesives(Si-PSAs)are valued for their thermal stability,flexibility,and biocompatibility,but their weak bonding strength restricts high-performance use.Polyurethane-modified Si-PSAs enhance adhesion,however diisocyanates remain essential.The raw materials of isocyanates are toxic,and their synthesis involves phosgene.To make up for those shortcomings,a series of poly(hydroxy urethane-siloxane)PSAs,named as PHUSi here,were synthesized through the ring-opening reaction of cyclic carbonate-functionalized polysiloxanes(PSi_(x)-VEC_(z))with various aliphatic diamines.The PSi_(x)-VEC_(z) precursors were prepared via the hydrosilylation of hydrogen-containing polysiloxanes(PSi_(x)-H_(y))with 4-vinyl-1,3-dioxolan-2-one(VEC).The chemical structures of PSi_(x)-H_(y),PSi_(x)-VEC_(z) and PHUSi were characterized,and bonding properties of PHUSi were systematically evaluated.The influence of architectures on adhesive performance was elucidated through comprehensive analyses,including rheology,crosslink density assessment,and so on.These studies revealed that the tailored design of PHUSi adhesives combine the advantages of traditional Si-PSAs with enhanced adhesion while eliminating isocyanate toxicity.The optimized PHUSi formulation achieved remarkable 180°peel strength(76.5 N/m on skin)and maximum probe tack force(1.61 N),enabling secure 24 h attachment of flexible sensors to skin.These properties make PHUSi particularly suitable for medical applications,as demonstrated by successful implementation in flexible electrocardiogram devices,offering a biocompatible,high-performance adhesive.
基金Funded by the National Natural Science Foundation of China(Nos.52278269,52278268,52178264)Tianjin Outstanding Young Scholars Science Fund Project(No.22JCJQJC00020)Key Project of Tianjin Natural Science Foundation(No.23JCZDJC00430)。
文摘The effect of antibacterial adhesive on the biological corrosion resistance of mortar in seawater environment was studied by means of scanning electron microscope,thermogravimetric analysis,X-ray diffraction,Fourier transform infrared spectroscopy,and ultra-depth microscope.The results show that the antibacterial adhesive can effectively inhibit the growth of sulfur-oxidizing bacteria in seawater,hinder their metabolism to produce biological sulfate,and reduce the formation of destructive product gypsum.The mineral composition and thermal analysis showed that the peak value of plaster diffraction peak and the mass loss of plaster dehydration in antibacterial adhesive group were significantly lower than those in blank group(without protective coating group).In addition,the electric flux of chloride ions(>400 C)in the blank group of mortar samples was higher than that in the antibacterial adhesive group(<200 C),indicating that the antibacterial adhesive can effectively reduce the permeability of chloride ions in mortar,and thus hinder the Cl-erosion in seawater.
基金supported by the National Natural Science Foundation of China(52222302)。
文摘Reuse/recycling of expensive components in next-generation optoelectronic systems(e.g.,flexible electronics and foldable displays)for sustainability purposes impose stringent requirements on optically clear adhesive(OCA)with on-demand adhesion/deadhesion capability.Although some hot-melt OCAs have been developed,there still remain great challenges in balancing the mechanical property,adhesion/detachment and recyclability/reusability of the OCAs.Herein,we developed a debondable and robust polyurethane(PU)-based optically clear adhesive with both mechanical and chemical recyclability,overcoming the critical bottlenecks in sustainable advanced manufacturing.The ductile yet strong PU-based OCAs not only can adhere to various optically transparent glass and polymeric substrates(with a bonding strength high up to 5.0 MPa),but also exhibit deadhesion capability at high temperature owing to the thermally sensitive H-bonding interaction within the polymer network.The ondemand adhesion/deadhesion facilitates non-destructive detachment of functional assemblies and materials recycling/repair.The mechanical and chemical recyclability of PU-based OCAs can also effectively reduce the cost and address the endof-life issues.Moreover,the PU-based OCAs outperformed some commercial optical adhesives.In view of the straightforward synthesis,easy industrial scale-up and exceptional comprehensive properties,the PU-based OCAs will provide promising transformative solutions for advancing the circular economy and sustainability in next-generation optoelectronic devices.
基金financially supported by the National Key Research and Development Project of China(No.2022YFB3806900)。
文摘The complex interactions and conflicting performance demands in multi-component composites pose significant challenges for achieving balanced multi-property optimization through conventional trial-and-error approaches.Machine learning(ML)offers a promising solution,markedly improving materials discovery efficiency.However,the high dimensionality of feature spaces in such systems has long impeded effective ML-driven feature representation and inverse design.To overcome this,we present an Intelligent Screening System(ISS)framework to accelerate the discovery of optimal formulations balancing four key properties in 15-component PTFE-based copper-clad laminate composites(PTFE-CCLCs).ISS adopts modular descriptors based on the physical information of component volume fractions,thereby simplifying the feature representation.By leveraging the inverse prediction capability of ML models and constructing a performance-driven virtual candidate database,ISS significantly reduced the computational complexity associated with high-dimensional spaces.Experimental validation confirmed that ISSoptimized formulations exhibited superior synergy,notably resolving the trade-off between thermal conductivity and peel strength,and outperform many commercial counterparts.Despite limited data and inherent process variability,ISS achieved an average prediction accuracy of 76.5%,with thermal conductivity predictions exceeding 90%,demonstrating robust reliability.This work provides an innovative,efficient strategy for multifunctional optimization and accelerated discovery in ultra-complex composite systems,highlighting the integration of ML and advanced materials design.
文摘Conducting hydrogels have garnered significant interest in the field of wearable electronics.However,simultaneously achieving high transparency,high conductivity,strong adhesion,and self-healing ability within a short time remains a major challenge.In this study,a multifunctional mussel-inspired hydrogel was synthesized in only 5 min,with polydopamine(PDA)-polypyrrole(Ppy)-polyaniline(PANi)and poly(vinyl alcohol)(PVA)nanoparticles incorporated into the polyacrylamide(PAM)network.The resulting hydrogel exhibited high transparency(about 90% light transmission in the range of 400-800 nm),high conductivity((95.4±0.4)×10^(-4)S/cm),tensile strength(32.60±1.03 k Pa),strain at break(904.46%±11.50%),and adhesive strength(30-60 k Pa).It also demonstrated rapid self-healing properties(about 48% strength recovery within 1h at 50℃)and water-dependent shape memory behavior.As a wearable strain sensor,the hydrogel successfully detected finger flexion,wrist movements,facial expression changes,and breathing with high sensitivity and stability.The calculated gauge factor(GF)was 7.44±0.31,which is higher than that of many previously reported hydrogels.Compared with previous oyster-inspired or Ppy-based hydrogels,our system showed a much shorter synthesis time,higher transparency,and enhanced multifunctionality.These findings highlight the potential of the proposed hydrogel for next-generation flexible electronics,e-skin,and biomedical monitoring devices.
基金financially supported by the Ministry of Science and Higher Education of the Russian Federation。
文摘Carbon-fiber-reinforced plastics(CFRP)with improved mechanical properties based on modified epoxy binders were investigated in this study.By adding 15 parts by weight(p.b.w.)of copolymer of polysulfone with cardo phthalide group(PSFP-70C)to the epoxyanhydride binder,the flexural strength of the epoxy polymer was increased by 60%,the CFRP based on it by 57%,the flexural modulus of the epoxy polymer was increased by 83%,and the composite by 96%.The adhesion strength of the binder to carbon fiber reached a high level at 10 p.b.w.of thermoplastic modifier and increased by 65%compared to the unmodified binder.Scanning electron microscopy(SEM)was used to determine that in epoxyanhydride systems with a polysulfone content of 5–15 p.b.w.,the structure belongs to the"matrix dispersion"type and with a content of 20 p.b.w.to the"interpenetrating phase"type.A heterogeneous structure was also observed using dynamic mechanical analysis.
基金supported by grants from the National Natural Science Foundation of China(82372881 to Weiyang He)the Chongqing Biomedicine Key R&D Project(CSTB2021TIAD-KPX0041 to Weiyang He).
文摘Objective:While cisplatin-based chemotherapy is pivotal for advanced bladder cancer,acquired resistance remains a major obstacle.This study investigates key molecular drivers of this resistance and potential reversal strategies.Methods:We established GC(Gemcitabine and Cisplatin)-resistant T24-R and UC3-R cell lines from T24 and UM-UC-3(UC3)cells.Transcriptomic and proteomic analyses identified differentially expressed molecules.Apoptosis and cell viability were assessed by flow cytometry and CCK-8(Cell Counting Kit-8)assays,while RT-qPCR(Reverse Transcription Quantitative Polymerase Chain Reaction)and Western blot analyzed gene and protein expression.Immunofluorescence evaluated FAK(Focal Adhesion Kinase)phosphorylation,and a xenograft mouse model validated the findings in vivo.Results:Integrated transcriptomic and proteomic analysis identified FN1(fibronectin)as a consistently upregulated top candidate in resistant cells(T24-R transcript log_(2)FC=2.8,protein log_(2)FC=0.9;UC3-R transcript log_(2)FC=3.7;all p<0.001).Knockdown of FN1 reduced chemoresistance(Resistance Index:5.2 in T24-R and 2.0 in UC3-R cells,p<0.001)and enhanced apoptosis(approximately 4.5-fold in T24-R and 7.5-fold in UC3-R,p<0.001).ITGB4(Integrin Subunit Beta 4)was upregulated in resistant cells(transcript log_(2)FC:4.2 in T24-R and 3.03 in UC3-R;protein log_(2)FC:0.67 in T24-R;all p<0.01).Critically,ITGB4 knockdown abolished the chemoresistance promoted by exogenous FN1,which was associated with increased FAK(Y397)phosphorylation.Conclusion:Our results demonstrate that the FN1-ITGB4 axis drives chemoresistance in bladder cancer via FAK signaling.Targeting this axis represents a promising strategy to overcome chemoresistance.
基金Project supported by the National Natural Science Foundation of China(Nos.12502117,12272269,11972257)the Natural Science Foundation of Ningxia of China(No.2024AAC03018)+1 种基金the Fundamental Research Funds for the Central Universitiesthe Shanghai Gaofeng Project for University Academic Program Development。
文摘Driven by the trend of device miniaturization and high-density integration,the interaction between adjacent electrodes has become a critical factor affecting the interfacial reliability of thermoelectric(TE)structures.This study investigates the influence of adjoining electrode interactions on the interfacial response of a multi-electrode/TE substrate structure,including interfacial stresses and stress intensity factors at the electrode ends.To solve the corresponding boundary-value problem,the Fourier transforms are adopted to derive a governing integro-differential equation for the interfacial shear stress in multi-electrode systems,incorporating the TE effects as generalized forces on the right-hand side.The results show that both the interfacial tension and transverse stress in the electrodes are significantly affected by the presence of adjacent electrodes.The interaction between neighboring electrodes diminishes as their spacing increases or when an adhesive interlayer is introduced.Furthermore,the softer and thinner electrodes,the softer and thicker adhesive interlayer,and the smaller TE loads are found to be beneficial for improving the interfacial performance.These findings may contribute to the accurate measurement in surface sensors and layout design of multi-point health monitoring systems for TE structures.
基金supported by European Union Funding Programme,PNRR,No. 760058(to DMH)the UEFISCDI Project,No. PN-III-P4-IDPCE-2020-059(to APW)
文摘The major aim of stroke therapy is to stimulate brain repair and improve behavioral recovery after cerebral ischemia.One option is to stimulate endogenous neurogenesis in the subventricular zone and direct the newly formed neurons to the damaged area.However,only a small percentage of these neurons survive,and many do not reach the damaged area,possibly because the corpus callosum impedes the migration of subventricular zone-derived stem cells into the lesioned cortex.A second major obstacle to stem cell therapy is the strong inflammatory reaction induced by cerebral ischemia,whereby the associated phagocytic activity of brain macrophages removes both therapeutic cells and/or cell-based drug carriers.To address these issues,neurogenesis was electrically stimulated in the subventricular zone,followed by isolation of proliferating cells,including newly formed neurons,which were subsequently mixed with a nutritional hydrogel.This mixture was then transferred to the stroke cavity of day 14 post-stroke mice.We found that the performance of the treated animals improved in behavioral tests,including novel object,open field,hole board,grooming,and“time-to-feel”adhesive tape tests.Furthermore,immunostaining revealed that the stem cell marker nestin,the neuroepithelial marker Mash1,and the immature neuronal marker doublecortin-positive cells survived in the transplanted area for 2 weeks,possibly due to reduced phagocytic activity and supportive angiogenesis.These results clearly indicate that the transplantation of committed subventricular zone stem cells combined with a protective nutritional gel directly into the infarct cavity after the peak of stroke-induced neuroinflammation represents a feasible approach to improve neurorestoration after cerebral ischemia.
文摘BACKGROUND Repeated application of the Pringle maneuver is a key obstacle to safe minimally invasive repeat liver resection(MISRLR).However,limited technical guidance is available.AIM To study the utility of newly developed Pringle taping method guided by liver surface in MISRLR.METHODS We retrospectively reviewed 72 cases of MISRLR performed by a single surgeon at two centers from August 2015 to July 2024.Beginning in October 2019,a liver surface-guided encirclement of hepatoduodenal ligament(LSEH)was used for repeat Pringle taping.Perioperative outcomes including Pringle taping success,operative time,blood loss,conversion rate,morbidity,and mortality were assessed.RESULTS Laparoscopic and robotic approaches were used in 63 patients and 9 patients,respectively.The median operative time,blood loss,and hospital stay were 331.5 minutes,70 mL,and 8 days,respectively.Open conversion occurred in two cases(2.8%)due to severe adhesions and right renal vein injury.Clavien-Dindo grade≥III complications occurred in 5.6%of cases with no mortality.Anti-adhesion barriers were used in 54 patients(75.0%).LSEH was attempted in 57 cases,improving Pringle taping success from 33.0%to 91.4%(P<0.001).LSEH succeeded in all patients with prior open liver resection(n=11).Among 6 patients in whom LSEH failed,3 patients(50.0%)had undergone a third liver resection,and 1 patient had a history of distal gastrectomy with choledochoduodenostomy.CONCLUSION The newly developed LSEH technique for Pringle taping in MISRLR was feasible,enhancing safety and reproducibility even in patients with a history of open liver resection.
基金This work was supported by the National Natural Science Foundation of China (Grant No. 39770700).
文摘Cellular immune response is a major barrier to xenotransplantation. Human tumor necrosis factor-α (hTNF-α) possesses cross-species activity and directly amplifies the immune rejection via the upregulation of adhesion molecules on porcine endothelium. We investigated the role of protein tyrosine phosphorylation in the induction of expression of E-selectin and vascular cell adhesion molecule-1 (VCAM-1), and the augmentation of adhesion of human peripheral blood monocytes (PBMo) and natural killer cells (PBNK), after rhTNF-α-stimulation of porcine aortic endo-thelial cells (PAEC) in vitro. rhTNF-α-increased adhesiveness of PAEC for both PBMo and PBNK was dose-dependently reduced by pretreatment of PAEC with the selective protein tyrosine kinase (PTK) inhibitor genistein. The inhibitory effect occurred at the early time of PAEC activation triggered by rhTNF-α, and was completely reversible. PTK activity assay indicated that genistein also suppressed rhTNF-α stimulated activation of protein tyrosine
基金supported by the Wisdom Accumulation and Talent Cultivation Project of Third Xiangya Hosipital of Central South University,China(YX202112).
文摘Objective:Endometrial tuberculosis,which commonly affects women of reproductive age,is a significant cause of intrauterine adhesions(IUA),potentially leading to hypomenorrhea,amenorrhea,and infertility.Hysteroscopic adhesiolysis is the primary treatment for IUA;however,studies specifically addressing its efficacy in tuberculosisinduced IUA remain scarce.This study aims to evaluate the therapeutic outcomes of hysteroscopic adhesiolysis for IUA caused by endometrial tuberculosis.Methods:This retrospective cohort study included patients diagnosed with tuberculosisinduced IUA who underwent hysteroscopic adhesiolysis at the Third Xiangya Hospital of Central South University between May 2014 and October 2022.Clinical data including age,medical history,adhesion severity,surgical treatment,and reproductive outcomes were analyzed.Results:Among 39 patients identified,2 were lost to follow-up.A total of 37 patients were included,with a follow-up duration ranging from 6 months to 9 years.Hypomenorrhea was reported in 24(64.9%)patients,secondary amenorrhea in 10(27.0%)patients,and normal menstruation in 3(8.1%)patients.Most patients presented with primary infertility(59.5%),and only 2(5.4%)had secondary infertility.The median American Fertility Society(AFS)score at initial assessment was 10(range,8−12);8(21.6%)patients had moderate IUA,and 29(78.4%)had severe IUA.A total of 86 surgical procedures were performed across 37 patients,with 27 patients undergoing 2 or more surgeries.Postoperatively,25(67.6%)patients achieved normalization of the uterine cavity,while 12(32.4%)still had a reduced cavity.Only 7(18.9%)patients had a grossly normal endometrium at the final surgery,all of whom had moderate adhesions at the initial procedure.Menstrual flow returned to normal in 12(32.4%)patients,while 25(67.6%)continued to experience hypomenorrhea.Of 29 patients who attempted in vitro fertilization and embryo transfer(IVF-ET),only 6(20.7%)conceived.Among these,4(13.8%)delivered at term via cesarean section;one case was complicated by postpartum hemorrhage due to uterine atony and another by placental adhesion.Conclusion:Endometrial tuberculosis can lead to severe IUA.Hysteroscopic adhesiolysis facilitates cavity restoration and improvement of menstrual conditions,but the overall reproductive outcomes remain suboptimal.
文摘This review is composed of three main parts each of which is written by well-known top specialists that have been,in a way or other,also the main participants of the majority of the developments reported.Thus,after a general part covering the grand lines and more in-depth views of more recent tannin,lignin,carbohydrate and soy bioadhesives,somemix of the other bio raw materials with soy protein and soy flour and some other differently sourced bioadhesives for wood,this review presents a more in-depth part on starch-based wood adhesives and a more indepth part covering plant protein-based adhesives.It must be kept in mind that the review is focused on completely or almost completely biosourced adhesives,the fashionable adhesives derived from mixes of biosourced materials with synthetic resins having been intentionally excluded.This choice was made as the latter constitute only an intermediate interval,possibly temporary if even for a somewhat long times,towards a final full bioeconomy of scale in this field.This review also focuses on more recent results,mainly obtained in the last 10–20 years,thus on adhesive formulations really innovative and sometimes even non-traditional.In all these fields there is still a lot of possibility of innovation for relevant formulation as this field is still in rapid growth.
基金supported by the National Natural Science Foundation of China(Nos.22273098,22373003,22103002 and 52033001)the Key Project of Anhui Province Science and Technology Innovation Platform(No.202305a12020030)the financial support from the Anhui Provincial Natural Science Foundation(No.2408085Y004)。
文摘Polymeric materials which can undergo controlled degradation and recycling are of great significance for a sustainable society.Although tremendous progress has been made in the degradation and recycling of both thermoplastic and thermoset plastics,the development of high-performance degradable polymer adhesives is rare.Here,we have prepared high-performance nucleobase-containing thioctic acid-based supramolecular polymer adhesives through free radical polymerization.The specific hydrogen-bonding interactions between complementary nucleobases greatly improve the weak cohesion of the thioctic acid-based polymers and enhance the environmental stability of the thioctic acidbased polymers simultaneously.Degradation of the nucleobase-containing thioctic acid-based supramolecular polymers is achieved by the reduction of the disulfide backbone,and the cycle of degradation and repolymerization is further achieved via oxidative polymerization.The adhesion strength of the nucleobase-containing thioctic acid-based supramolecular polymers after two cycles of degradation and repolymerization still reaches as high as 4.7±0.3 MPa.This work provides an approach for the development of environmentally stable and high-performance degradable thioctic acid-based adhesives.
