含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-4(a disintegrin-like and metalloproteinase with thrombospondin type-1 motifs-4, ADAMTS-4)是ADAMTS家族中的一个重要成员。ADAMTS-4可能通过降解细胞外基质、促进炎症反应、促进...含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-4(a disintegrin-like and metalloproteinase with thrombospondin type-1 motifs-4, ADAMTS-4)是ADAMTS家族中的一个重要成员。ADAMTS-4可能通过降解细胞外基质、促进炎症反应、促进平滑肌细胞迁移等多种途径参与心血管疾病的发生发展过程。本文分别介绍了ADAMTS-4与动脉粥样硬化、冠心病、心力衰竭、主动脉瘤及主动脉夹层的关系,分析了ADAMTS-4在动脉粥样硬化、冠心病、心力衰竭、主动脉瘤及主动脉夹层中的作用。ADAMTS-4有望成为心血管疾病诊治的标志物及作用靶点,为心血管疾病的诊断与治疗提供了新的依据,为未来心血管疾病诊治拓宽了思路。展开更多
目的探讨妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)患者血清及胎盘组织中人类含I型血小板结合蛋白基序的解聚蛋白样金属蛋白酶1(human depolymerizin-like metalloproteinase-1 containing the type I platelet...目的探讨妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)患者血清及胎盘组织中人类含I型血小板结合蛋白基序的解聚蛋白样金属蛋白酶1(human depolymerizin-like metalloproteinase-1 containing the type I platelet-binding protein motif,ADAMTS-1)和ADAMTS-4的表达水平。方法选择2021年7月至2022年3月苏北人民医院产科收治的妊娠晚期ICP患者(ICP组,22例)和同期住院正常待产孕妇(对照组,20例),采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)和免疫组化试验(immunohistochemistry,IHC)分别分析ADAMTS-1和ADAMTS-4的表达水平,比较两组血清及胎盘组织中ADAMTS-1和ADAMTS-4表达水平的差异。结果ICP组年龄24~34岁,其中初产妇12例,经产妇10例。对照组年龄21~34岁,其中初产妇11例,经产妇9例。与对照组相比,ICP组平均产时孕周提前([38.0±1.5)周比(39.7±0.9)周]、血清总胆汁酸(total bile acid,TBA)较高([27.33±16.94)μmol/L比(2.85±1.58)μmol/L]、血清ADAMTS-1较高([49.41±4.39)ng/ml比(33.01±3.13)ng/ml]、血清ADAMTS-4较高([134.47±17.47)ng/ml比(87.39±12.35)ng/ml],差异均有统计学意义(P<0.05)。与对照组相比,ICP组胎盘组织ADAMTS-1和ADAMTS-4强阳性比例较高(36.36%比10%,22.73%比15.00%),差异均有统计学意义(P<0.05)。结论ADAMTS-1、ADAMTS-4在ICP患者血清及胎盘中的表达明显增加,提示二者可能参与ICP的发生发展。展开更多
含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-4(A disintegrin-like and metalloproteinase with thrombospondin type-1motifs-4,ADAMTS-4)属于ADAMTS金属蛋白酶家族中的一员,主要由巨噬细胞分泌,由无活性的酶原经过切割转化为具...含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-4(A disintegrin-like and metalloproteinase with thrombospondin type-1motifs-4,ADAMTS-4)属于ADAMTS金属蛋白酶家族中的一员,主要由巨噬细胞分泌,由无活性的酶原经过切割转化为具有活性的酶在细胞外发挥作用,ADATMS-4 mRNA表达受IFN-γ、TNF-α、IL-1β、IL-1、IL-6、TGF-β等多种因子的调控,参与了关节炎、骨关节病、癌症、心脏病等多种疾病的发生发展过程。作为一种新型炎症因子,ADAMTS-4在动脉粥样硬化的发展中起了重要作用,对动脉粥硬化斑块的不稳定性有影响,并且其在急性心肌梗死中的表达也有变化。因此,ADAMTS-4与冠状动脉粥样硬化性心脏病有着密切联系。文章就ADAMTS-4与冠心病的研究进展做一综述。展开更多
Objective:To observe the clinical effect of electroacupuncture"Tongmin point"combined with heat-sensitive moxibustion on knee osteoarthritis and its effect on blood lipid level,polyprotein polysaccharide enz...Objective:To observe the clinical effect of electroacupuncture"Tongmin point"combined with heat-sensitive moxibustion on knee osteoarthritis and its effect on blood lipid level,polyprotein polysaccharide enzyme-4(a disintegrin and metalloprotease with thrombospondin motifs,ADAMTS-4,matrix metalloproteinase-3(matrix metalloproteinase-3,);and MMPs-3)Impact.Methods:80 patients were randomly divided into two groups,40 cases per group,Treatment and control groups,The treatment group was given electroacupuncture combined with heat-sensitive moxibustion,Six times a week,Continuous treatment for 4 weeks;The control group was given intraarticular sodium hyaluronate,Once a week,continuous treatment for 4 weeks.Results:During the treatment period,Visual analogue scale visual analogue scale,VAS)show a downward trend,Intra-group comparisons,Differences were statistically significant(P<0.05);After 2 weeks of treatment,4 weeks of treatment and 2 weeks of follow-up,All the scores in the treatment group were lower than those in the control group(P<0.05).After treatment in two groups(The Western Ontario and Mc Master Universities Osteoarthritis Index,)of osteoarthritis index,University of Western Ontario WOMAC)The scores of each item decreased significantly,treatment group was better than the control group(P<0.05).After four weeks of treatment,(Total cholesterol,)Total cholesterol TC,triglycerides(Triglyceride,);and TG,LDL(Low density lipoprotein,);and LDL-C)A decrease in content,High density lipoprotein High density lipoprotein,HDL-C)Increased content,and the improvement was better than that of the control group(P<0.05).After treatment,the ADAMTS-4,MMPs-3 content decreased,Intergroup comparisons,Differences were statistically significant(P<0.05);The effective rate in the treatment group was 87.5%,The control rate was 60%,treatment group was more effective(P<0.05).Conclusions:Electroacupuncture"Tongmin point"combined with heat-sensitive moxibustion can obviously improve the symptoms of knee osteoarthritis patients,Regulate blood lipids,High security,It is worth popularizing in clinic.展开更多
Objective:To observe the expression of CTX-I(Ⅰcollagen C-terminal foreign body peptide)and TRACP(tartrate-resistant acid phosphatase)in different syndromes of knee osteoarthritis and their correlation with ADAMTS-4(p...Objective:To observe the expression of CTX-I(Ⅰcollagen C-terminal foreign body peptide)and TRACP(tartrate-resistant acid phosphatase)in different syndromes of knee osteoarthritis and their correlation with ADAMTS-4(polyproteoglycan enzyme-4)and MMPS-3(matrix metalloproteinase-3).Methods:A total of 98 patients diagnosed with knee osteoarthritis were enrolled from the Rehabilitation Pain Department and Rheumatology Department of Wuhan Caidian District People's Hospital.According to their syndrome types,they were divided into 40 cases of Yang deficiency and cold constipation type,28 cases of qi stagnation and blood stasis type,and 30 cases of kidney jing deficiency type.The contents of bone metabolism indexes,ADAMTS-4 and MMPS-3 in different syndromes of the three groups were compared.Correlation of bone metabolism indexes with ADAMTS-4 and MMPS-3.Results:There were statistically significant differences in the contents of CTX-I,TRACP,ADAMTS-4 and MMPS-3 among three groups with different syndromes(P<0.05).There was a positive correlation between CTX-I,TRACP,ADAMTS-4 and MMPS-3(P<0.05).The syndrome of qi stagnation and blood stasis was positively correlated with TRACP and ADAMTS-4,and negatively correlated with MMPS-3(P<0.05).The renal essence deficiency syndrome was positively correlated with CTX-I,ADAMTS-4 and MMPS-3(P<0.05).Bone metabolic indexes(CTX-I,TRACP)were positively correlated with ADAMTS-4 and MMPS-3(P<0.05).Conclusion:Different syndromes of knee osteoarthritis are correlated with bone metabolism indexes,ADAMTS-4 and MMPS-3,which can provide a certain objective basis for clinical TCM syndrome differentiation and classification。展开更多
目的:观察电针“痛敏穴”联合热敏灸对膝骨性关节炎的临床疗效及对血脂水平、聚蛋白多糖酶4(a disintegrin and metalloprotease with thrombospondin motifs,ADAMTS-4)、基质金属蛋白酶-3(matrix metalloproteinase-3,MMPs-3)影响。方...目的:观察电针“痛敏穴”联合热敏灸对膝骨性关节炎的临床疗效及对血脂水平、聚蛋白多糖酶4(a disintegrin and metalloprotease with thrombospondin motifs,ADAMTS-4)、基质金属蛋白酶-3(matrix metalloproteinase-3,MMPs-3)影响。方法:将80例膝骨性关节炎患者随机分为治疗组40例与对照组40例。治疗组给予电针联合热敏灸,每周6次,连续治疗4周;对照组给予关节腔内注射玻璃酸钠,每周1次,连续治疗4周。结果:两组在治疗期,视觉模拟评分量表(visual analogue scale,VAS)均呈现下降趋势,组内比较,差异具有统计学意义(P<0.05);在治疗2周、治疗4周、随访2周,治疗组得分均低于对照组(P<0.05)。两组治疗后(美国西安大略麦马斯特大学骨性关节炎指标(Western Ontario and McMaster Universities osteoarthritis index,WOMAC)各项目得分均明显下降,治疗组疗效优于对照组(P<0.05)。治疗组在4周治疗后,总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(LDL-C)含量下降,高密度脂蛋白胆固醇(HDL-C)含量上升,其改善程度优于对照组(P<0.05)。两组治疗后ADAMTS-4、MMPs-3含量均下降,组间比较,差异有明显统计学意义(P<0.05);治疗组有效率为87.5%,对照组有效率为60.0%,治疗组疗效更佳(P<0.05)。结论:电针“痛敏穴”联合热敏灸能明显改善膝骨性关节炎患者的症状,调节血脂水平,安全性高,值得临床推广使用。展开更多
文摘含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-4(a disintegrin-like and metalloproteinase with thrombospondin type-1 motifs-4, ADAMTS-4)是ADAMTS家族中的一个重要成员。ADAMTS-4可能通过降解细胞外基质、促进炎症反应、促进平滑肌细胞迁移等多种途径参与心血管疾病的发生发展过程。本文分别介绍了ADAMTS-4与动脉粥样硬化、冠心病、心力衰竭、主动脉瘤及主动脉夹层的关系,分析了ADAMTS-4在动脉粥样硬化、冠心病、心力衰竭、主动脉瘤及主动脉夹层中的作用。ADAMTS-4有望成为心血管疾病诊治的标志物及作用靶点,为心血管疾病的诊断与治疗提供了新的依据,为未来心血管疾病诊治拓宽了思路。
文摘目的探讨妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)患者血清及胎盘组织中人类含I型血小板结合蛋白基序的解聚蛋白样金属蛋白酶1(human depolymerizin-like metalloproteinase-1 containing the type I platelet-binding protein motif,ADAMTS-1)和ADAMTS-4的表达水平。方法选择2021年7月至2022年3月苏北人民医院产科收治的妊娠晚期ICP患者(ICP组,22例)和同期住院正常待产孕妇(对照组,20例),采用酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)和免疫组化试验(immunohistochemistry,IHC)分别分析ADAMTS-1和ADAMTS-4的表达水平,比较两组血清及胎盘组织中ADAMTS-1和ADAMTS-4表达水平的差异。结果ICP组年龄24~34岁,其中初产妇12例,经产妇10例。对照组年龄21~34岁,其中初产妇11例,经产妇9例。与对照组相比,ICP组平均产时孕周提前([38.0±1.5)周比(39.7±0.9)周]、血清总胆汁酸(total bile acid,TBA)较高([27.33±16.94)μmol/L比(2.85±1.58)μmol/L]、血清ADAMTS-1较高([49.41±4.39)ng/ml比(33.01±3.13)ng/ml]、血清ADAMTS-4较高([134.47±17.47)ng/ml比(87.39±12.35)ng/ml],差异均有统计学意义(P<0.05)。与对照组相比,ICP组胎盘组织ADAMTS-1和ADAMTS-4强阳性比例较高(36.36%比10%,22.73%比15.00%),差异均有统计学意义(P<0.05)。结论ADAMTS-1、ADAMTS-4在ICP患者血清及胎盘中的表达明显增加,提示二者可能参与ICP的发生发展。
文摘含Ⅰ型血小板结合蛋白基序的解聚蛋白样金属蛋白酶-4(A disintegrin-like and metalloproteinase with thrombospondin type-1motifs-4,ADAMTS-4)属于ADAMTS金属蛋白酶家族中的一员,主要由巨噬细胞分泌,由无活性的酶原经过切割转化为具有活性的酶在细胞外发挥作用,ADATMS-4 mRNA表达受IFN-γ、TNF-α、IL-1β、IL-1、IL-6、TGF-β等多种因子的调控,参与了关节炎、骨关节病、癌症、心脏病等多种疾病的发生发展过程。作为一种新型炎症因子,ADAMTS-4在动脉粥样硬化的发展中起了重要作用,对动脉粥硬化斑块的不稳定性有影响,并且其在急性心肌梗死中的表达也有变化。因此,ADAMTS-4与冠状动脉粥样硬化性心脏病有着密切联系。文章就ADAMTS-4与冠心病的研究进展做一综述。
基金Clinical Medical Research Project of Wuhan Municipal Health and Family Planning Commission(No.WZ20Q12)Major project of the National Natural Science Foundation of China"Clinical Application Research on Different Functional States of Acupoints"(No.81590955)。
文摘Objective:To observe the clinical effect of electroacupuncture"Tongmin point"combined with heat-sensitive moxibustion on knee osteoarthritis and its effect on blood lipid level,polyprotein polysaccharide enzyme-4(a disintegrin and metalloprotease with thrombospondin motifs,ADAMTS-4,matrix metalloproteinase-3(matrix metalloproteinase-3,);and MMPs-3)Impact.Methods:80 patients were randomly divided into two groups,40 cases per group,Treatment and control groups,The treatment group was given electroacupuncture combined with heat-sensitive moxibustion,Six times a week,Continuous treatment for 4 weeks;The control group was given intraarticular sodium hyaluronate,Once a week,continuous treatment for 4 weeks.Results:During the treatment period,Visual analogue scale visual analogue scale,VAS)show a downward trend,Intra-group comparisons,Differences were statistically significant(P<0.05);After 2 weeks of treatment,4 weeks of treatment and 2 weeks of follow-up,All the scores in the treatment group were lower than those in the control group(P<0.05).After treatment in two groups(The Western Ontario and Mc Master Universities Osteoarthritis Index,)of osteoarthritis index,University of Western Ontario WOMAC)The scores of each item decreased significantly,treatment group was better than the control group(P<0.05).After four weeks of treatment,(Total cholesterol,)Total cholesterol TC,triglycerides(Triglyceride,);and TG,LDL(Low density lipoprotein,);and LDL-C)A decrease in content,High density lipoprotein High density lipoprotein,HDL-C)Increased content,and the improvement was better than that of the control group(P<0.05).After treatment,the ADAMTS-4,MMPs-3 content decreased,Intergroup comparisons,Differences were statistically significant(P<0.05);The effective rate in the treatment group was 87.5%,The control rate was 60%,treatment group was more effective(P<0.05).Conclusions:Electroacupuncture"Tongmin point"combined with heat-sensitive moxibustion can obviously improve the symptoms of knee osteoarthritis patients,Regulate blood lipids,High security,It is worth popularizing in clinic.
基金Research Project Funding of Health and Family Planning Commission of Wuhan(No.WZ20Q12)Key Program of the National Natural Science Fund of China(No.81590955)。
文摘Objective:To observe the expression of CTX-I(Ⅰcollagen C-terminal foreign body peptide)and TRACP(tartrate-resistant acid phosphatase)in different syndromes of knee osteoarthritis and their correlation with ADAMTS-4(polyproteoglycan enzyme-4)and MMPS-3(matrix metalloproteinase-3).Methods:A total of 98 patients diagnosed with knee osteoarthritis were enrolled from the Rehabilitation Pain Department and Rheumatology Department of Wuhan Caidian District People's Hospital.According to their syndrome types,they were divided into 40 cases of Yang deficiency and cold constipation type,28 cases of qi stagnation and blood stasis type,and 30 cases of kidney jing deficiency type.The contents of bone metabolism indexes,ADAMTS-4 and MMPS-3 in different syndromes of the three groups were compared.Correlation of bone metabolism indexes with ADAMTS-4 and MMPS-3.Results:There were statistically significant differences in the contents of CTX-I,TRACP,ADAMTS-4 and MMPS-3 among three groups with different syndromes(P<0.05).There was a positive correlation between CTX-I,TRACP,ADAMTS-4 and MMPS-3(P<0.05).The syndrome of qi stagnation and blood stasis was positively correlated with TRACP and ADAMTS-4,and negatively correlated with MMPS-3(P<0.05).The renal essence deficiency syndrome was positively correlated with CTX-I,ADAMTS-4 and MMPS-3(P<0.05).Bone metabolic indexes(CTX-I,TRACP)were positively correlated with ADAMTS-4 and MMPS-3(P<0.05).Conclusion:Different syndromes of knee osteoarthritis are correlated with bone metabolism indexes,ADAMTS-4 and MMPS-3,which can provide a certain objective basis for clinical TCM syndrome differentiation and classification。
