Biochemical changes of natural actomyosin from fresh pale, soft, exudative (PSE) and normal pork were studied,and the effects of different storage temperatures and different incubation temperature and times on sample ...Biochemical changes of natural actomyosin from fresh pale, soft, exudative (PSE) and normal pork were studied,and the effects of different storage temperatures and different incubation temperature and times on sample superprecipitation,total sulfhydryl (-SH) content, and ATP (adenosine triphosphate) sensitivity were investigated. The results demonstrated that ATPase activity and thermal stability of PSE actomyosin were lower than those of normal pork; and that PSE actomyosin had higher -SH content than that of normal pork at all incubation temperatures and times tested.展开更多
Conidiation is the primary mode of reproduction in filamentous fungi and is essential for the dispersal of pathogenic species.However,the fundamental cellular mechanisms regulating conidiation in plant pathogenic fung...Conidiation is the primary mode of reproduction in filamentous fungi and is essential for the dispersal of pathogenic species.However,the fundamental cellular mechanisms regulating conidiation in plant pathogenic fungi remain largely unexplored.Here,using Verticillium dahliae as a model,we investigated the dynamic assembly and function of the contractile actomyosin ring(CAR)and septins during conidiation through live-cell imaging.We show that septins,visualized via VdCdc11-GFP,first accumulate at the tip of budding hyphae during the transition from hyphal elongation to apical budding,and undergo an hourglass-to-double-ring transition at the bud neck.Following mitosis,myosin Ⅱ and actin assemble simultaneously into a contractile ring to drive cytokinesis.Disruption of core septin function results in defective nuclear segregation and aberrant nuclear migration during mitosis,as well as delayed recruitment of myosin Ⅱ to the bud neck,indicating that septins scaffold cytokinetic machinery and coordinate nuclear division during conidiation.In contrast,during hyphal septation,myosin Ⅱ,actin,and septins appear simultaneously as a diffuse cortical band,with septin organization dependent on actin.Collectively,these findings reveal distinct spatial and temporal coordination between actomyosin and septins in two cytokinetic contexts-conidiation and hyphal septation-and define apical budding as a specialized cytokinesis mode in V.dahliae.Our study broadens the understanding of fungal cytokinesis beyond yeast models to multicellular filamentous fungi.展开更多
基金Project (No. 200019) supported by the Natural Science Foundation of Zhejiang Province,China
文摘Biochemical changes of natural actomyosin from fresh pale, soft, exudative (PSE) and normal pork were studied,and the effects of different storage temperatures and different incubation temperature and times on sample superprecipitation,total sulfhydryl (-SH) content, and ATP (adenosine triphosphate) sensitivity were investigated. The results demonstrated that ATPase activity and thermal stability of PSE actomyosin were lower than those of normal pork; and that PSE actomyosin had higher -SH content than that of normal pork at all incubation temperatures and times tested.
基金supported by the National Science Foundation of China(Grant No.32370204)the State Key Laboratory of Microbial Diversity and Innovative Utilization.
文摘Conidiation is the primary mode of reproduction in filamentous fungi and is essential for the dispersal of pathogenic species.However,the fundamental cellular mechanisms regulating conidiation in plant pathogenic fungi remain largely unexplored.Here,using Verticillium dahliae as a model,we investigated the dynamic assembly and function of the contractile actomyosin ring(CAR)and septins during conidiation through live-cell imaging.We show that septins,visualized via VdCdc11-GFP,first accumulate at the tip of budding hyphae during the transition from hyphal elongation to apical budding,and undergo an hourglass-to-double-ring transition at the bud neck.Following mitosis,myosin Ⅱ and actin assemble simultaneously into a contractile ring to drive cytokinesis.Disruption of core septin function results in defective nuclear segregation and aberrant nuclear migration during mitosis,as well as delayed recruitment of myosin Ⅱ to the bud neck,indicating that septins scaffold cytokinetic machinery and coordinate nuclear division during conidiation.In contrast,during hyphal septation,myosin Ⅱ,actin,and septins appear simultaneously as a diffuse cortical band,with septin organization dependent on actin.Collectively,these findings reveal distinct spatial and temporal coordination between actomyosin and septins in two cytokinetic contexts-conidiation and hyphal septation-and define apical budding as a specialized cytokinesis mode in V.dahliae.Our study broadens the understanding of fungal cytokinesis beyond yeast models to multicellular filamentous fungi.
