Lin Wei is a hiking enthusiast.At six o'clock on the last Saturday morning,the temperature at the foot of the mountain was only 2℃,so she put on her thickest fleece jacket.However,after only half an hour of climb...Lin Wei is a hiking enthusiast.At six o'clock on the last Saturday morning,the temperature at the foot of the mountain was only 2℃,so she put on her thickest fleece jacket.However,after only half an hour of climbing,the heat left her drenched in sweat,making her feel very cold.By midday,the temperature was approaching 20℃,and her heavy jacket had to be tied around her waist,becoming a burden during her hike.This outdoor adventure allowed her to appreciate the beautiful scenery,but also subjected her to repeated changes in temperature.展开更多
Introduction:The choice is no longer whether—but when and how!Today,organizations can no longer choose whether or not to engage with stakeholders;the only real decision is when and how to do it successfully.Engaging ...Introduction:The choice is no longer whether—but when and how!Today,organizations can no longer choose whether or not to engage with stakeholders;the only real decision is when and how to do it successfully.Engaging stakeholders is essential for any type of organization,public,private,or civil society.In fact,it is a fundamental part of effective governance,continuous improvement,and social responsibility.The principle behind stakeholder engagement is simple yet powerful:those who can influence or be affected by an organization's mission must be given the opportunity to express their views and contribute to shaping the decisions that impact them.展开更多
Prevention of biological invasion requires understanding how alien species invade native communities.Although studies have identified mechanisms that underlie plant invasion in some habitats,limited attention has focu...Prevention of biological invasion requires understanding how alien species invade native communities.Although studies have identified mechanisms that underlie plant invasion in some habitats,limited attention has focused on invasion patterns along elevational gradients.In this study,we asked which factors drive the global and regional distribution of the invasive plant Galinsoga quadriradiata along elevational gradients.To answer this question,we examined whether human activities(i.e.,roads)promote G.quadriradiata invasion,how seed dispersal-related traits of G.quadriradiata change along elevation gradients,and whether G.quadriradiata has adapted to high-elevation environments through phenotypic plasticity or genetic variation.On the global scale,we found that human activities and road density positively contribute to the G.quadriradiata expansion in mountainous areas.Field surveys in China revealed significant elevational differences in the seed dispersal traits of G.quadriradiata,with higher-elevation populations exhibiting lower dispersal ability and generally lower genetic diversity.Under common conditions,high-elevation populations showed higher leaf mass ratio but lower root mass ratio and reproductive allocation.This suggests that high-elevation environments create a barrier to dispersal for G.quadriradiata,and that G.quadriradiata has adapted phenotypically to these conditions.Our study indicates that the elevational invasion pattern of G.quadriradiata is shaped by multiple factors,particularly human activities and phenotypic adaptability.In addition,our finding that G.quadriradiata invasion at high elevations is not constrained by low genetic diversity indicates that monitoring and management of G.quadriradiata in mountainous areas should be strengthened.展开更多
Human activities have significantly impacted the land surface temperature(LST),endangering human health;however,the relationship between these two factors has not been adequately quantified.This study comprehensively ...Human activities have significantly impacted the land surface temperature(LST),endangering human health;however,the relationship between these two factors has not been adequately quantified.This study comprehensively constructs a Human Activity Intensity(HAI)index and employs the Maximal Information Coefficient,four-quadrant model,and XGBoostSHAP model to investigate the spatiotemporal relationship and influencing factors of HAI-LST in the Yellow River Basin(YRB)from 2000 to 2020.The results indicated that from 2000 to 2020,as HAI and LST increased,the static HAI-LST relationship in the YRB showed a positive correlation that continued to strengthen.This dynamic relationship exhibited conflicting development,with the proportion of coordinated to conflicting regions shifting from 1:4 to 1:2,indicating a reduction in conflict intensity.Notably,only the degree of conflict in the source area decreased significantly,whereas it intensified in the upper and lower reaches.The key factors influencing the HAI-LST relationship include fractional vegetation cover,slope,precipitation,and evapotranspiration,along with region-specific factors such as PM_(2.5),biodiversity,and elevation.Based on these findings,region-specific ecological management strategies have been proposed to mitigate conflict-prone areas and alleviate thermal stress,thereby providing important guidance for promoting harmonious development between humans and nature.展开更多
Passiflora incarnata L.,commonly known as passionflower,is traditionally cultivated as an ornamental plant but has demonstrated diverse therapeutic potential.Its pharmacological effects are attributed to bioactive com...Passiflora incarnata L.,commonly known as passionflower,is traditionally cultivated as an ornamental plant but has demonstrated diverse therapeutic potential.Its pharmacological effects are attributed to bioactive compounds such as flavonoids and alkaloids,which influence multiple biological pathways.This review aims to summarise and critically analyse recent findings on the pharmacological properties of Passiflora incarnata L.,focusing on its neuropsychiatric,antioxidant,antimicrobial,and anticancer activities.A targeted literature search was conducted in PubMed,Scopus,Web of Science,and Google Scholar for peer-reviewed publications between 2000 to 2025.Relevant articles were screened,and a more appropriate article related to the objective of the review was selected.Some classical papers are also cited as per the requirement of the topic.Passiflora incarnata L.showed multifunctional medicinal properties with various applications in neuropsychiatry,oxidative stress management,antimicrobial agent,and as an anticancer agent.The U.S.Food and Drug Administration categorizes passionflower extracts as“generally recognized as safe”.However,most evidence remains preclinical,with methodological variation limiting generalisation.Standardised formulation,robust clinical trials,and in-depth in vivo studies are essential to establish its therapeutic relevance and safety in modern medicine.展开更多
Ruscogenin,a steroidal sapogenin,is primarily derived from the genus Ruscus.It demonstrates a wide range of pharmacological actions that hold substantial promise for therapeutic use.By conducting a comprehensive exami...Ruscogenin,a steroidal sapogenin,is primarily derived from the genus Ruscus.It demonstrates a wide range of pharmacological actions that hold substantial promise for therapeutic use.By conducting a comprehensive examination of electronic databases including PubMed,ScienceDirect,and SciFinder,we identified studies on ruscogenin to date.This review aims at emphasizing the findings related to the bioactivities of ruscogenin,particularly its protective activities in neurological and inflammatory disorders,hemorrhoids,and cancer.Ruscogenin has been demonstrated to possess anti-ulcer,anti-osteoporotic,and skin-lightening properties as a therapeutic agent.It has also been proven effective in managing metabolic disorders by reducing inflammation and improving lipid metabolism,particularly in conditions such as non-alcoholic steatohepatitis and diabetic nephropathy.Reports indicate that it can prevent acute lung injury by protecting pulmonary endothelial cells from apoptosis and modulating inflammatory cytokines.Additionally,ruscogenin promotes neurological recovery by mitigating oxidative stress.It also shows anti-cancer properties,leading to apoptosis and hindering metastasis,particularly in pancreatic and hepatocellular carcinoma.This review summarizes the potential medical applications of ruscogenin and underscores the need for further research on its mechanisms of action.展开更多
Panax notoginseng(P.notoginseng),a valuable traditional Chinese medicine,is the dried root of plants in Acanthopanax gracilistylus family,with the effect of dispersing blood stasis,eliminating swelling and relieving p...Panax notoginseng(P.notoginseng),a valuable traditional Chinese medicine,is the dried root of plants in Acanthopanax gracilistylus family,with the effect of dispersing blood stasis,eliminating swelling and relieving pain.With the development of modern medicine,the active ingredients and mechanisms of P.notoginseng have been gradually revealed.The present paper systematically reviews the chemical composition and biological activities of P.nologinseng,to provide a scientific basis and reference for detailed research on P.nologinseng.展开更多
Rarer dementias are associated with atypical symptoms and younger onset,which result in a higher burden of care.We provide a review of the global literature on longitudinal decline in activities of daily living(ADLs)i...Rarer dementias are associated with atypical symptoms and younger onset,which result in a higher burden of care.We provide a review of the global literature on longitudinal decline in activities of daily living(ADLs)in dementias that account for less than 10%of dementia diagnoses.Published studies were identified through searches conducted in Medical Literature Analysis and Retrieval System Online(MEDLINE),Excerpta Medica Database(Embase),Excerpta Medica Care(Emcare),PsycINFO,and Cumulative Index in Nursing and Allied Health Literature(CINAHL).The search criteria included terms related to‘rarer dementias’,‘activities of daily living’and‘longitudinal or cross-sectional studies’following a predefined protocol registered.Studies were screened,and those that met the criteria were citation searched.Quality assessments were performed,and relevant data were extracted.20 articles were selected,of which 19 focused on dementias within the frontotemporal dementia/primary progressive aphasia spectrum,while one addressed posterior cortical atrophy.Four studies were cross-sectional and 16 studies were longitudinal,with a median duration of 2.2 years.The Disability Assessment for Dementia was used to measure decline in 8 of the 20 studies.The varied sequences of ADL decline reported in the literature reflect variation in diagnostic specificity between studies and within-syndrome heterogeneity.Most studies used Alzheimer’s disease staging scales to measure decline,which cannot capture variant-specific symptoms.To enhance care provision in dementia,ADL scales could be deployed postdiagnosis to aid treatment and planning.This necessitates staging scales that are variant-specific and span the disease course from diagnosis to end of life.展开更多
To explore the potential utilization of Elaeagnus mollis,we conducted a comprehensive assessment of its phytochemical composition,antioxidant properties,cholinesterase inhibition,and anti-HepG2 cell proliferation acti...To explore the potential utilization of Elaeagnus mollis,we conducted a comprehensive assessment of its phytochemical composition,antioxidant properties,cholinesterase inhibition,and anti-HepG2 cell proliferation activity across different plant parts(branch wood,branch bark,and pericarp)using various solvents(water,methanol,ethanol,and n-hexane).Our findings revealed that water extracts displayed superior antioxidant activities in ABTS and RP assays,while methanol extracts exhibited better performance in DPPH and FRAP assays.Moreover,methanol extracts demonstrated the highest effectiveness against anti-HepG2 cell proliferation,whereas n-hexane extracts showed greater efficiency in cholinesterase inhibition.Notably,branch bark extracts exhibited the highest levels of phytochemical compounds,with both branch bark and pericarp extracts demonstrating significant effects in cholinesterase inhibition and anti-HepG2 cell proliferation.Correlation analysis indicated that phytochemical compounds were primarily responsible for the observed biological activities.Overall,extracts from the branch bark and pericarp of E.mollis showed promising potential for antioxidant and anticancer activities,suggesting their suitability for applications in the pharmaceutical industry as health-promoting products.展开更多
Carbon fluxes are essential indicators assessing vegetation carbon cycle functions.However,the extent and mechanisms by which climate change and human activities influence the spatiotemporal dynamics of carbon fluxes ...Carbon fluxes are essential indicators assessing vegetation carbon cycle functions.However,the extent and mechanisms by which climate change and human activities influence the spatiotemporal dynamics of carbon fluxes in arid oasis and non-oasis area remains unclear.Here,we assessed and predicted the future effects of climate change and human activities on carbon fluxes in the Hexi Corridor.The results showed that the annual average gross primary productivity(GPP),net ecosystem productivity(NEP),and ecosystem respiration(Reco)in the Hexi Corridor oasis increased by 263.91 g C·m^(-2)·yr^(-1),118.45 g C·m^(-2)·yr^(-1)and 122.46 g C·m^(-2)·yr^(-1),respectively,due to the expansion of the oasis area by 3424.84 km^(2) caused by human activities from 2000 to 2022.Both oasis and non-oasis arid ecosystems in the Hexi Corridor acted as carbon sinks.Compared to the non-oasis area,the carbon fluxes contributions of oasis area increased,ranging from 10.21%to 13.99%for GPP,8.50%to11.68%for NEP,and 13.34%to 17.13%for Reco.The contribution of the carbon flux from the oasis expansion area to the total carbon flux change in the Hexi Corridor was 30.96%(7.09 Tg C yr^(-1))for GPP,29.57%(3.39 Tg C yr^(-1))for NEP and 32.40%(3.58 Tg C yr^(-1))for Reco.The changes in carbon fluxes in the oasis area were mainly attributed to human activities(oasis expansion)and temperature,whereas non-oasis area was mainly due to climate factors.Moreover,the future increasing trends were observed for GPP(64.99%),NEP(66.29%)and Reco(82.08%)in the Hexi Corridor.This study provides new insights into the regulatory mechanisms of carbon cycle in the arid oasis and non-oasis area.展开更多
Impairments on body function,activities of daily living(ADL)and cognition are common after stroke.Eccentric resistance training(ERT)may be implemented to improve them.The primary objectives were to evaluate whether ER...Impairments on body function,activities of daily living(ADL)and cognition are common after stroke.Eccentric resistance training(ERT)may be implemented to improve them.The primary objectives were to evaluate whether ERT improves body function,ADL and cognition after stroke.The secondary objectives were to evaluate whether ERT improves strength,gait,quality of life,and self-perceived health.Seven electronic databases were searched:Cochrane Central Register of Controlled Trials,Cochrane Stroke Group Trials Register,PubMed,Epistemonikos,Embase,SPORTDiscus,and WHO International Clinical Trials Registry Platform.Last search was run in December 2023,including studies since 2012.Selection criteria were studies with stroke participants of both sexes,aged 18 or more,with an intervention based on ERT.Accepted languages were English,Spanish or French.First search was done in pairs.Authors removed duplicate studies and those which did not meet inclusion criteria through title and abstracts.Finally,all authors,independently,screened the final search results and extracted data.Of 68 records identified,15 were eligible and 6 were finally included(with moderate risk of bias),analyzing 159 participants.Body function(4 interventions,n=84),gait(4 interventions,n=115)and strength(3 interventions,n?78)showed significantly better results when ERT was performed.Meta-analysis could not be done because of the few studies and their heterogeneity.This review provided low-moderate quality evidence suggesting that ERT might be effective at improving body function,strength,and gait after stroke.Besides,no harm was documented,and it was well-accepted.展开更多
Objective:To explore the barriers and facilitators associated with the implementation of horticultural activities(HA)in nursing care homes,and aim to identify findings that will enhance the development of more effecti...Objective:To explore the barriers and facilitators associated with the implementation of horticultural activities(HA)in nursing care homes,and aim to identify findings that will enhance the development of more effective implementation strategies for such activities.Methods:This qualitative descriptive study recruited a purposive sample of potential adopters related to HA in nursing care homes,and the conventional content analysis method was adopted.Results:A total of 6 older adults,3 social workers,1 nurse,and 1 physician participated in this study.The analysis revealed 4 themes barriers and 2 themes facilitators to the implementation of HA.Conclusions:Future intervention programs should be meticulously designed to address the identified influencing factors,and the insights gained can serve as a reference for identifying and mitigating barriers in the implementation of other physical activity programs.In turn,this will contribute to improved physical functioning and healthy aging.展开更多
A novel low molecular weight compound polysaccharide(LMW-CPS) was identified from a specific combination of Chinese herb ingredients.The monosaccharide composition of LMW-CPS was consisted of single arabinose,which ha...A novel low molecular weight compound polysaccharide(LMW-CPS) was identified from a specific combination of Chinese herb ingredients.The monosaccharide composition of LMW-CPS was consisted of single arabinose,which had an α-L-furanose configuration with an average molecular weight of 2.06 kDa.NMR spectra and monosaccharide constitution analyses revealed that it had a backbone of→5)-α-L-Araf-(1→with α-L-Araf(1→as the terminal residue.In vitro experiments found that it could lead to apoptosis and inhibit hepatocellular carcinoma cell proliferation by arresting them in the S phase.In vivo experiments showed that it protected immune organs such as the thymus and spleen,enhanced immune cell activities,stimulated cytokine release,augmented the abundance of CD8,CD3,CD4,and CD 19 positive lymphocytes,and markedly impeded solid hepatocellular carcinoma progression in mice.Hematoxylin and eosin staining and cell cycle examination also indicated that LMW-CPS arrested hepatocellular carcinoma cells at the S phase to induce apoptosis.These findings indicated its promising potential for the treatment of hepatocellular carcinoma.展开更多
Myopia,a common cause of visual impairment,together with the global decline in physical fitness and increasing prevalence of childhood obesity,has become a prominent global health problem.The beneficial effect of incr...Myopia,a common cause of visual impairment,together with the global decline in physical fitness and increasing prevalence of childhood obesity,has become a prominent global health problem.The beneficial effect of increasing the time of outdoor activities on the incidence of myopia and physical well-being in children has been widely recognized.However,in countries with highly competitive education systems,such as China,parents and school administrators may be reluctant to increase their children's time for extracurricular outdoor activities for fear of affecting their academic performance.Therefore,it is crucial to accurately assess the role of after-school outdoor activities in preventing and controlling myopia,as well as in promoting the physical and mental development of adolescents.Schools and families should be encouraged to collaboratively support children’s engagement in outdoor activities to foster their healthy growth.Given the dual benefits of outdoor activities-improving visual health and promoting overall physical and mental well-being-it is essential to encourage schools and families to work together to support children's engagement in such activities.By doing so,it is hoped that a balanced environment can be created,one that values both academic achievement and healthy development.Policymakers,educators,and parents must recognize the long-term advantages of integrating outdoor activities into children's daily lives,as this approach can help alleviate the burden of myopia,enhance physical fitness,and support emotional and cognitive development.Ultimately,fostering a culture that views outdoor activities as an essential component of education and health will be key to ensuring the well-rounded development of future generations.展开更多
With the rapid urbanization process,ground collapses caused by anthropogenic activities occur frequently.Accurate susceptibility mapping is of great significance for disaster prevention and control.In this study,1198 ...With the rapid urbanization process,ground collapses caused by anthropogenic activities occur frequently.Accurate susceptibility mapping is of great significance for disaster prevention and control.In this study,1198 ground collapse cases in Shenzhen from 2017 to 2020 were collected.Eight effective factors(elevation,relief,clay proportion,average annual precipitation,distance from water,land use type,building density,and road density)were selected to construct the evaluation index system.Ground collapse susceptibility was analyzed and mapped using the normalized frequency ratio(NFR),logistic regression(LR),and NFR-LR coupling models.Finally,the result rationality and performance of the three models were compared through frequency ratio(FR)and ROC curve.The results indicate that all three models can effectively evaluate the ground collapse susceptibility(AUC>0.7),and the NFR-LR model result is more rational and has the best performance(AUC=0.791).The very high and high susceptibility zones cover a total area of 545.68 km^(2) and involve Nanshan,Luohu,and Futian District,as well as some areas of Baoan,Guangming,and Longgang District.The ground collapses in Shenzhen mainly occurred in the built-up areas,and the greater intensity of anthropogenic activities,the more susceptible to the disaster.展开更多
Airborne microorganisms(AM)have significant environmental and health implications.Extensive studies have been conducted to investigate the factors influencing the composition and diversity of AM.However,the knowledge ...Airborne microorganisms(AM)have significant environmental and health implications.Extensive studies have been conducted to investigate the factors influencing the composition and diversity of AM.However,the knowledge of AM with anthropogenic activities has not reach a consensus.In this study,we took advantage of the dramatic decline of outdoor anthropogenic activities resulting from COVID-19 lockdown to reveal their associations.We collected airborne particulate matter before and during the lockdown period in two cities.The results showed that it was fungal diversity and communities but not bacteria obviously different between pre-lockdownand lockdown samples,suggesting that airborne fungiwere more susceptible to anthropogenic activities than bacteria.However,after the implementation of lockdown,the co-occurrence networks of both bacterial and fungal community became more complex,whichmight be due to the variation of microbial sources.Furthermore,Mantel test and correlation analysis showed that air pollutants also partly contributed to microbial alterations.Airborne fungal community was more affected by air pollutants than bacterial community.Notably,some human pathogens like Nigrospora and Arthrinium were negatively correlated with air pollutants.Overall,our study highlighted the more impacts of anthropogenic activities on airborne fungal community than bacterial community and advanced the understanding of associations between anthropogenic activities and AM.展开更多
This work aims to compare the chemical composition and anti-inflammatory effects on RAW264.7 macrophages of Keemun black tea stems and leaves.A total of 50 volatile compounds were identified in tea stems and leaves,an...This work aims to compare the chemical composition and anti-inflammatory effects on RAW264.7 macrophages of Keemun black tea stems and leaves.A total of 50 volatile compounds were identified in tea stems and leaves,and aldehydes,alcohols,and esters were the main volatile compound categories.There were 11 key volatile compounds,including geraniol,benzeneacetaldehyde,methyl salicylate,linalool,etc.contributed to distinguishing the tea stems from the tea leaves.In the quantitative and liquid chromatography-mass spectrometry(LC-MS)-based metabolomics analysis,higher contents of amino acids,monosaccharides,and quinic acids were found in stems than those in leaves.Inversely,higher contents of tea pigments,flavan-3-ols,gallic acid,purine alkaloids,and flavonol glycosides were present in tea leaves than in stems.LC-MS-based metabolomics also revealed that organic acids were the most critical non-volatile compounds responsible for the differences between tea stems and leaves.Furthermore,tea stems had better inhibiting effects of pro-inflammatory cytokines(interleukin(IL)-1βand IL-6)in lipopolysaccharide-challenged RAW264.7 macrophages than tea leaves,while no significant differences exist between leaves and stems for inhibiting the secretion of tumor necrosis factorα(TNF-α)and NO.In conclusion,our results support using Keemun black tea stems as a novel source of anti-inflammatory compounds.展开更多
Practical activities are an indispensable piece of science education.As such,assessment of these practical activities is vital for continually enhancing the quality of science instruction.This article presents a tenta...Practical activities are an indispensable piece of science education.As such,assessment of these practical activities is vital for continually enhancing the quality of science instruction.This article presents a tentative framework structured for assessing practical activities in science education.The proposed framework is built upon seven components:engagement and participation,relational thinking,stepwise mastery,contextualization,multidimensional integration,empowerment,and scientific articulacy.The framework might provide science educators with an alternative lens through which to enhance the effectiveness of practical activities,promote student engagement,and better prepare learners for the future.This framework is designed to be additive and complementary to existing assessment approaches and can be adapted and customized to suit specific local contexts and varying grade levels by science educators in the pursuit of excellence in an ever-evolving science education landscape.展开更多
Central Asia(CA)faces escalating threats from increasing temperature,glacier retreat,biodiversity loss,unsustainable water use,terminal lake shrinkage,and soil salinization,all of which challenge the balance between e...Central Asia(CA)faces escalating threats from increasing temperature,glacier retreat,biodiversity loss,unsustainable water use,terminal lake shrinkage,and soil salinization,all of which challenge the balance between ecological integrity and socio-economic development essential for achieving Sustainable Development Goals.However,a comprehensive understanding of priority areas from a multi-dimensional perspective is lacking,hindering effective conservation and development strategies.To address this,we developed a comprehensive assessment framework with a tailored indicator system,enabling a spatial evaluation of CA’s priority areas by integrating biodiversity,ecosystem services(ESs),and human activities.Combining zonation and geographical detectors,this approach facilitates spatial prioritization and examines ecological and socio-economic heterogeneity.Our findings reveal a heterogeneous distribution of priority areas across CA,with significant concentrations in eastern mountainous regions,river valleys,and oasis agricultural lands.We identified 184 key districts crucial for ecological and societal sustainability.Attribution analysis shows that natural factors like soil types,precipitation,and evapotranspiration significantly shape these areas,influencing human activities and the distribution of biodiversity and ESs.Multi-dimensional analysis indicates existing protected areas cover only 15%of the top 30%priority areas,revealing substantial conservation gaps.Additionally,a 38%overlap between ESs and human activities,along with 63.25%congruence in integrated areas,underscores significant human impacts on ecological systems and their dependency on ESs.Given CA’s limited resources,it is crucial to implement measures that strengthen conservation efforts,align ecological preservation with socio-economic demands,and enhance resource efficiency through sustainable integrated land and water resource management.展开更多
The physicochemical properties,structural characteristics,antioxidant,radioprotective and lipid-lowering activities,as well as the underlying structure-activity relationships of polysaccharides extracted from Nostoc f...The physicochemical properties,structural characteristics,antioxidant,radioprotective and lipid-lowering activities,as well as the underlying structure-activity relationships of polysaccharides extracted from Nostoc flagelliforme grown under normal(WL-EPS-1),salt stress(NaCl-EPS-1)and mixotrophic culture conditions(Glu-EPS-1)were studied.The results demonstrated that WL-EPS-1,NaCl-EPS-1 and Glu-EPS-1 were heteropolysaccharides comprising different proportions of monosaccharides and uronic acid,with different average molecular weights of 0.99×10^(3),1.09×10^(3)and 1.18×10^(3)kDa,respectively.Their intrinsic viscosity were significantly different,at 24.72,29.98,and 41.06 dL/g,respectively.The functional groups of polysaccharides were not greatly affected,but the chemical composition,triple-helix structure,chain length and surface morphology were significantly influenced by culture conditions.In vitro bioactivity assays showed that the antioxidant activity generally increased in the order of WL-EPS-1<NaCl-EPS-1<Glu-EPS-1,while NaCl-EPS-1 had the best radioprotective effect,and Glu-EPS-1 had the best lipid-lowering effect.In addition,the structure-activity relationship of the polysaccharides was analyzed by partial least squares,which revealed that the most important factors affecting the antioxidant,radioprotective and lipid-lowering activities of polysaccharides were viscosity and molecular weight.This study provides a strategy for obtaining high-bioactivity polysaccharides by appropriate regulation of culture conditions,as well as opening new directions for the molecular modification of polysaccharides.展开更多
文摘Lin Wei is a hiking enthusiast.At six o'clock on the last Saturday morning,the temperature at the foot of the mountain was only 2℃,so she put on her thickest fleece jacket.However,after only half an hour of climbing,the heat left her drenched in sweat,making her feel very cold.By midday,the temperature was approaching 20℃,and her heavy jacket had to be tied around her waist,becoming a burden during her hike.This outdoor adventure allowed her to appreciate the beautiful scenery,but also subjected her to repeated changes in temperature.
文摘Introduction:The choice is no longer whether—but when and how!Today,organizations can no longer choose whether or not to engage with stakeholders;the only real decision is when and how to do it successfully.Engaging stakeholders is essential for any type of organization,public,private,or civil society.In fact,it is a fundamental part of effective governance,continuous improvement,and social responsibility.The principle behind stakeholder engagement is simple yet powerful:those who can influence or be affected by an organization's mission must be given the opportunity to express their views and contribute to shaping the decisions that impact them.
基金supported by the National Natural Science Foundation of China(32271584 and 31600445)the Natural Science Basic Research Plan in Shaanxi Province of China(2020JM-286)+2 种基金the Fundamental Research Funds for the Central Universities(GK202103072,GK202103073)the National College Students'Innovative Entrepreneurial Training Plan Program(202310718085)Special Research Project in Philosophy and Social Sciences of Shaanxi Province(2022HZ1795).
文摘Prevention of biological invasion requires understanding how alien species invade native communities.Although studies have identified mechanisms that underlie plant invasion in some habitats,limited attention has focused on invasion patterns along elevational gradients.In this study,we asked which factors drive the global and regional distribution of the invasive plant Galinsoga quadriradiata along elevational gradients.To answer this question,we examined whether human activities(i.e.,roads)promote G.quadriradiata invasion,how seed dispersal-related traits of G.quadriradiata change along elevation gradients,and whether G.quadriradiata has adapted to high-elevation environments through phenotypic plasticity or genetic variation.On the global scale,we found that human activities and road density positively contribute to the G.quadriradiata expansion in mountainous areas.Field surveys in China revealed significant elevational differences in the seed dispersal traits of G.quadriradiata,with higher-elevation populations exhibiting lower dispersal ability and generally lower genetic diversity.Under common conditions,high-elevation populations showed higher leaf mass ratio but lower root mass ratio and reproductive allocation.This suggests that high-elevation environments create a barrier to dispersal for G.quadriradiata,and that G.quadriradiata has adapted phenotypically to these conditions.Our study indicates that the elevational invasion pattern of G.quadriradiata is shaped by multiple factors,particularly human activities and phenotypic adaptability.In addition,our finding that G.quadriradiata invasion at high elevations is not constrained by low genetic diversity indicates that monitoring and management of G.quadriradiata in mountainous areas should be strengthened.
基金Shanxi Province Graduate Research Practice Innovation Project,No.2023KY465Project on the Reform of Graduate Education and Teaching in Shanxi Province,No.2021YJJG146+1 种基金Research Project of Shanxi Provincial Cultural Relics Bureau,No.22-8-14-1400-119National Key R&D Program of China,No.2021YFB3901300。
文摘Human activities have significantly impacted the land surface temperature(LST),endangering human health;however,the relationship between these two factors has not been adequately quantified.This study comprehensively constructs a Human Activity Intensity(HAI)index and employs the Maximal Information Coefficient,four-quadrant model,and XGBoostSHAP model to investigate the spatiotemporal relationship and influencing factors of HAI-LST in the Yellow River Basin(YRB)from 2000 to 2020.The results indicated that from 2000 to 2020,as HAI and LST increased,the static HAI-LST relationship in the YRB showed a positive correlation that continued to strengthen.This dynamic relationship exhibited conflicting development,with the proportion of coordinated to conflicting regions shifting from 1:4 to 1:2,indicating a reduction in conflict intensity.Notably,only the degree of conflict in the source area decreased significantly,whereas it intensified in the upper and lower reaches.The key factors influencing the HAI-LST relationship include fractional vegetation cover,slope,precipitation,and evapotranspiration,along with region-specific factors such as PM_(2.5),biodiversity,and elevation.Based on these findings,region-specific ecological management strategies have been proposed to mitigate conflict-prone areas and alleviate thermal stress,thereby providing important guidance for promoting harmonious development between humans and nature.
文摘Passiflora incarnata L.,commonly known as passionflower,is traditionally cultivated as an ornamental plant but has demonstrated diverse therapeutic potential.Its pharmacological effects are attributed to bioactive compounds such as flavonoids and alkaloids,which influence multiple biological pathways.This review aims to summarise and critically analyse recent findings on the pharmacological properties of Passiflora incarnata L.,focusing on its neuropsychiatric,antioxidant,antimicrobial,and anticancer activities.A targeted literature search was conducted in PubMed,Scopus,Web of Science,and Google Scholar for peer-reviewed publications between 2000 to 2025.Relevant articles were screened,and a more appropriate article related to the objective of the review was selected.Some classical papers are also cited as per the requirement of the topic.Passiflora incarnata L.showed multifunctional medicinal properties with various applications in neuropsychiatry,oxidative stress management,antimicrobial agent,and as an anticancer agent.The U.S.Food and Drug Administration categorizes passionflower extracts as“generally recognized as safe”.However,most evidence remains preclinical,with methodological variation limiting generalisation.Standardised formulation,robust clinical trials,and in-depth in vivo studies are essential to establish its therapeutic relevance and safety in modern medicine.
文摘Ruscogenin,a steroidal sapogenin,is primarily derived from the genus Ruscus.It demonstrates a wide range of pharmacological actions that hold substantial promise for therapeutic use.By conducting a comprehensive examination of electronic databases including PubMed,ScienceDirect,and SciFinder,we identified studies on ruscogenin to date.This review aims at emphasizing the findings related to the bioactivities of ruscogenin,particularly its protective activities in neurological and inflammatory disorders,hemorrhoids,and cancer.Ruscogenin has been demonstrated to possess anti-ulcer,anti-osteoporotic,and skin-lightening properties as a therapeutic agent.It has also been proven effective in managing metabolic disorders by reducing inflammation and improving lipid metabolism,particularly in conditions such as non-alcoholic steatohepatitis and diabetic nephropathy.Reports indicate that it can prevent acute lung injury by protecting pulmonary endothelial cells from apoptosis and modulating inflammatory cytokines.Additionally,ruscogenin promotes neurological recovery by mitigating oxidative stress.It also shows anti-cancer properties,leading to apoptosis and hindering metastasis,particularly in pancreatic and hepatocellular carcinoma.This review summarizes the potential medical applications of ruscogenin and underscores the need for further research on its mechanisms of action.
文摘Panax notoginseng(P.notoginseng),a valuable traditional Chinese medicine,is the dried root of plants in Acanthopanax gracilistylus family,with the effect of dispersing blood stasis,eliminating swelling and relieving pain.With the development of modern medicine,the active ingredients and mechanisms of P.notoginseng have been gradually revealed.The present paper systematically reviews the chemical composition and biological activities of P.nologinseng,to provide a scientific basis and reference for detailed research on P.nologinseng.
基金supported by UKResearch and Innovation(MR/S03546X/1)National BrainAppeal,Economic and Social ResearchCouncil(ES/S010467/1)+4 种基金Wellcome Trust(221915/Z/20/Z),ESRC(ES/W006014/1)Royal National Institute for Deaf People-Dunhill Medical Trust Pauline Ashley(204841/Z/16/Z,PA23)London Hospitals Biomedical Research Centre(221915/Z/20/Z)Bloomsbury and East London Doctoral Training Partnership(ES/P000592/1)National Institute for Health Research.
文摘Rarer dementias are associated with atypical symptoms and younger onset,which result in a higher burden of care.We provide a review of the global literature on longitudinal decline in activities of daily living(ADLs)in dementias that account for less than 10%of dementia diagnoses.Published studies were identified through searches conducted in Medical Literature Analysis and Retrieval System Online(MEDLINE),Excerpta Medica Database(Embase),Excerpta Medica Care(Emcare),PsycINFO,and Cumulative Index in Nursing and Allied Health Literature(CINAHL).The search criteria included terms related to‘rarer dementias’,‘activities of daily living’and‘longitudinal or cross-sectional studies’following a predefined protocol registered.Studies were screened,and those that met the criteria were citation searched.Quality assessments were performed,and relevant data were extracted.20 articles were selected,of which 19 focused on dementias within the frontotemporal dementia/primary progressive aphasia spectrum,while one addressed posterior cortical atrophy.Four studies were cross-sectional and 16 studies were longitudinal,with a median duration of 2.2 years.The Disability Assessment for Dementia was used to measure decline in 8 of the 20 studies.The varied sequences of ADL decline reported in the literature reflect variation in diagnostic specificity between studies and within-syndrome heterogeneity.Most studies used Alzheimer’s disease staging scales to measure decline,which cannot capture variant-specific symptoms.To enhance care provision in dementia,ADL scales could be deployed postdiagnosis to aid treatment and planning.This necessitates staging scales that are variant-specific and span the disease course from diagnosis to end of life.
基金National Natural Science Foundation of China(Grant No.31600549).
文摘To explore the potential utilization of Elaeagnus mollis,we conducted a comprehensive assessment of its phytochemical composition,antioxidant properties,cholinesterase inhibition,and anti-HepG2 cell proliferation activity across different plant parts(branch wood,branch bark,and pericarp)using various solvents(water,methanol,ethanol,and n-hexane).Our findings revealed that water extracts displayed superior antioxidant activities in ABTS and RP assays,while methanol extracts exhibited better performance in DPPH and FRAP assays.Moreover,methanol extracts demonstrated the highest effectiveness against anti-HepG2 cell proliferation,whereas n-hexane extracts showed greater efficiency in cholinesterase inhibition.Notably,branch bark extracts exhibited the highest levels of phytochemical compounds,with both branch bark and pericarp extracts demonstrating significant effects in cholinesterase inhibition and anti-HepG2 cell proliferation.Correlation analysis indicated that phytochemical compounds were primarily responsible for the observed biological activities.Overall,extracts from the branch bark and pericarp of E.mollis showed promising potential for antioxidant and anticancer activities,suggesting their suitability for applications in the pharmaceutical industry as health-promoting products.
基金The Foundation for Distinguished Young Scholars of Gansu Province,No.22JR5RA046Key Research Program of Gansu Province,No.23ZDKA0004+2 种基金The Joint Funds of the National Natural Science Foundation of China,No.U22A202690Interdisciplinary Youth Team Project from the Key Laboratory of Cryospheric Science and Frozen Soil Engineering,No.CSFSE-ZQ-2408The Youth Innovation Promotion Association CAS to X.W.,No.2020422。
文摘Carbon fluxes are essential indicators assessing vegetation carbon cycle functions.However,the extent and mechanisms by which climate change and human activities influence the spatiotemporal dynamics of carbon fluxes in arid oasis and non-oasis area remains unclear.Here,we assessed and predicted the future effects of climate change and human activities on carbon fluxes in the Hexi Corridor.The results showed that the annual average gross primary productivity(GPP),net ecosystem productivity(NEP),and ecosystem respiration(Reco)in the Hexi Corridor oasis increased by 263.91 g C·m^(-2)·yr^(-1),118.45 g C·m^(-2)·yr^(-1)and 122.46 g C·m^(-2)·yr^(-1),respectively,due to the expansion of the oasis area by 3424.84 km^(2) caused by human activities from 2000 to 2022.Both oasis and non-oasis arid ecosystems in the Hexi Corridor acted as carbon sinks.Compared to the non-oasis area,the carbon fluxes contributions of oasis area increased,ranging from 10.21%to 13.99%for GPP,8.50%to11.68%for NEP,and 13.34%to 17.13%for Reco.The contribution of the carbon flux from the oasis expansion area to the total carbon flux change in the Hexi Corridor was 30.96%(7.09 Tg C yr^(-1))for GPP,29.57%(3.39 Tg C yr^(-1))for NEP and 32.40%(3.58 Tg C yr^(-1))for Reco.The changes in carbon fluxes in the oasis area were mainly attributed to human activities(oasis expansion)and temperature,whereas non-oasis area was mainly due to climate factors.Moreover,the future increasing trends were observed for GPP(64.99%),NEP(66.29%)and Reco(82.08%)in the Hexi Corridor.This study provides new insights into the regulatory mechanisms of carbon cycle in the arid oasis and non-oasis area.
文摘Impairments on body function,activities of daily living(ADL)and cognition are common after stroke.Eccentric resistance training(ERT)may be implemented to improve them.The primary objectives were to evaluate whether ERT improves body function,ADL and cognition after stroke.The secondary objectives were to evaluate whether ERT improves strength,gait,quality of life,and self-perceived health.Seven electronic databases were searched:Cochrane Central Register of Controlled Trials,Cochrane Stroke Group Trials Register,PubMed,Epistemonikos,Embase,SPORTDiscus,and WHO International Clinical Trials Registry Platform.Last search was run in December 2023,including studies since 2012.Selection criteria were studies with stroke participants of both sexes,aged 18 or more,with an intervention based on ERT.Accepted languages were English,Spanish or French.First search was done in pairs.Authors removed duplicate studies and those which did not meet inclusion criteria through title and abstracts.Finally,all authors,independently,screened the final search results and extracted data.Of 68 records identified,15 were eligible and 6 were finally included(with moderate risk of bias),analyzing 159 participants.Body function(4 interventions,n=84),gait(4 interventions,n=115)and strength(3 interventions,n?78)showed significantly better results when ERT was performed.Meta-analysis could not be done because of the few studies and their heterogeneity.This review provided low-moderate quality evidence suggesting that ERT might be effective at improving body function,strength,and gait after stroke.Besides,no harm was documented,and it was well-accepted.
基金supported by the Science and Technology Research Project and funded by the Provincial-Ministry Co-construction Project(No.SBGJ202102186).
文摘Objective:To explore the barriers and facilitators associated with the implementation of horticultural activities(HA)in nursing care homes,and aim to identify findings that will enhance the development of more effective implementation strategies for such activities.Methods:This qualitative descriptive study recruited a purposive sample of potential adopters related to HA in nursing care homes,and the conventional content analysis method was adopted.Results:A total of 6 older adults,3 social workers,1 nurse,and 1 physician participated in this study.The analysis revealed 4 themes barriers and 2 themes facilitators to the implementation of HA.Conclusions:Future intervention programs should be meticulously designed to address the identified influencing factors,and the insights gained can serve as a reference for identifying and mitigating barriers in the implementation of other physical activity programs.In turn,this will contribute to improved physical functioning and healthy aging.
基金supported by the National Key Research and Development Program of China (2022YFF1100904)the Tianjin Key R&D Program (21YFSNSN00110)+2 种基金the Science and Technology Planning Project of State Administration for Market Regulation (2019MK005, 2020MK010, 2022MK012)Tianjin Administration for Market Regulation (2019-W20)State Criteria for Food Safety (spaq-2020-08, spaq-2020-31, spaq-2021-07, spaq-2022-05)。
文摘A novel low molecular weight compound polysaccharide(LMW-CPS) was identified from a specific combination of Chinese herb ingredients.The monosaccharide composition of LMW-CPS was consisted of single arabinose,which had an α-L-furanose configuration with an average molecular weight of 2.06 kDa.NMR spectra and monosaccharide constitution analyses revealed that it had a backbone of→5)-α-L-Araf-(1→with α-L-Araf(1→as the terminal residue.In vitro experiments found that it could lead to apoptosis and inhibit hepatocellular carcinoma cell proliferation by arresting them in the S phase.In vivo experiments showed that it protected immune organs such as the thymus and spleen,enhanced immune cell activities,stimulated cytokine release,augmented the abundance of CD8,CD3,CD4,and CD 19 positive lymphocytes,and markedly impeded solid hepatocellular carcinoma progression in mice.Hematoxylin and eosin staining and cell cycle examination also indicated that LMW-CPS arrested hepatocellular carcinoma cells at the S phase to induce apoptosis.These findings indicated its promising potential for the treatment of hepatocellular carcinoma.
文摘Myopia,a common cause of visual impairment,together with the global decline in physical fitness and increasing prevalence of childhood obesity,has become a prominent global health problem.The beneficial effect of increasing the time of outdoor activities on the incidence of myopia and physical well-being in children has been widely recognized.However,in countries with highly competitive education systems,such as China,parents and school administrators may be reluctant to increase their children's time for extracurricular outdoor activities for fear of affecting their academic performance.Therefore,it is crucial to accurately assess the role of after-school outdoor activities in preventing and controlling myopia,as well as in promoting the physical and mental development of adolescents.Schools and families should be encouraged to collaboratively support children’s engagement in outdoor activities to foster their healthy growth.Given the dual benefits of outdoor activities-improving visual health and promoting overall physical and mental well-being-it is essential to encourage schools and families to work together to support children's engagement in such activities.By doing so,it is hoped that a balanced environment can be created,one that values both academic achievement and healthy development.Policymakers,educators,and parents must recognize the long-term advantages of integrating outdoor activities into children's daily lives,as this approach can help alleviate the burden of myopia,enhance physical fitness,and support emotional and cognitive development.Ultimately,fostering a culture that views outdoor activities as an essential component of education and health will be key to ensuring the well-rounded development of future generations.
基金jointed supported by the National Natural Science Foundation of China(Nos.41920104007,41731284)。
文摘With the rapid urbanization process,ground collapses caused by anthropogenic activities occur frequently.Accurate susceptibility mapping is of great significance for disaster prevention and control.In this study,1198 ground collapse cases in Shenzhen from 2017 to 2020 were collected.Eight effective factors(elevation,relief,clay proportion,average annual precipitation,distance from water,land use type,building density,and road density)were selected to construct the evaluation index system.Ground collapse susceptibility was analyzed and mapped using the normalized frequency ratio(NFR),logistic regression(LR),and NFR-LR coupling models.Finally,the result rationality and performance of the three models were compared through frequency ratio(FR)and ROC curve.The results indicate that all three models can effectively evaluate the ground collapse susceptibility(AUC>0.7),and the NFR-LR model result is more rational and has the best performance(AUC=0.791).The very high and high susceptibility zones cover a total area of 545.68 km^(2) and involve Nanshan,Luohu,and Futian District,as well as some areas of Baoan,Guangming,and Longgang District.The ground collapses in Shenzhen mainly occurred in the built-up areas,and the greater intensity of anthropogenic activities,the more susceptible to the disaster.
基金supported by the National Natural Science Foundation of China(No.31900106)the East China University of Technology Practical Teaching Construction Project(No.DHSY-202261)。
文摘Airborne microorganisms(AM)have significant environmental and health implications.Extensive studies have been conducted to investigate the factors influencing the composition and diversity of AM.However,the knowledge of AM with anthropogenic activities has not reach a consensus.In this study,we took advantage of the dramatic decline of outdoor anthropogenic activities resulting from COVID-19 lockdown to reveal their associations.We collected airborne particulate matter before and during the lockdown period in two cities.The results showed that it was fungal diversity and communities but not bacteria obviously different between pre-lockdownand lockdown samples,suggesting that airborne fungiwere more susceptible to anthropogenic activities than bacteria.However,after the implementation of lockdown,the co-occurrence networks of both bacterial and fungal community became more complex,whichmight be due to the variation of microbial sources.Furthermore,Mantel test and correlation analysis showed that air pollutants also partly contributed to microbial alterations.Airborne fungal community was more affected by air pollutants than bacterial community.Notably,some human pathogens like Nigrospora and Arthrinium were negatively correlated with air pollutants.Overall,our study highlighted the more impacts of anthropogenic activities on airborne fungal community than bacterial community and advanced the understanding of associations between anthropogenic activities and AM.
基金supported by the Natural Science Foundation of China(32122079,32072633)Earmarked Fund for China Agriculture Research System(CARS-19)+2 种基金Anhui Key Research and Development Plan(202104b11020001)Young Elite Scientist Sponsorship Program by National CAST(2016QNRC001)High-level Introduced Talent Sponsorship Program by Anhui Agricultural University(rc352203)。
文摘This work aims to compare the chemical composition and anti-inflammatory effects on RAW264.7 macrophages of Keemun black tea stems and leaves.A total of 50 volatile compounds were identified in tea stems and leaves,and aldehydes,alcohols,and esters were the main volatile compound categories.There were 11 key volatile compounds,including geraniol,benzeneacetaldehyde,methyl salicylate,linalool,etc.contributed to distinguishing the tea stems from the tea leaves.In the quantitative and liquid chromatography-mass spectrometry(LC-MS)-based metabolomics analysis,higher contents of amino acids,monosaccharides,and quinic acids were found in stems than those in leaves.Inversely,higher contents of tea pigments,flavan-3-ols,gallic acid,purine alkaloids,and flavonol glycosides were present in tea leaves than in stems.LC-MS-based metabolomics also revealed that organic acids were the most critical non-volatile compounds responsible for the differences between tea stems and leaves.Furthermore,tea stems had better inhibiting effects of pro-inflammatory cytokines(interleukin(IL)-1βand IL-6)in lipopolysaccharide-challenged RAW264.7 macrophages than tea leaves,while no significant differences exist between leaves and stems for inhibiting the secretion of tumor necrosis factorα(TNF-α)and NO.In conclusion,our results support using Keemun black tea stems as a novel source of anti-inflammatory compounds.
基金supported by the Scientific Research Program of Hefei Normal University(2024KY71)the Partnership Program of Hefei Normal University(HXXM2022094).
文摘Practical activities are an indispensable piece of science education.As such,assessment of these practical activities is vital for continually enhancing the quality of science instruction.This article presents a tentative framework structured for assessing practical activities in science education.The proposed framework is built upon seven components:engagement and participation,relational thinking,stepwise mastery,contextualization,multidimensional integration,empowerment,and scientific articulacy.The framework might provide science educators with an alternative lens through which to enhance the effectiveness of practical activities,promote student engagement,and better prepare learners for the future.This framework is designed to be additive and complementary to existing assessment approaches and can be adapted and customized to suit specific local contexts and varying grade levels by science educators in the pursuit of excellence in an ever-evolving science education landscape.
基金funded by the Joint CAS-MPG Research Project(HZXM20225001MI)this research was also supported partly by the key program of National Natural Science Foundation of China(42230708)the Tianshan Talent Project of Xinjiang Uygur Autonomous Region,China(2022TSYCLJ0056).
文摘Central Asia(CA)faces escalating threats from increasing temperature,glacier retreat,biodiversity loss,unsustainable water use,terminal lake shrinkage,and soil salinization,all of which challenge the balance between ecological integrity and socio-economic development essential for achieving Sustainable Development Goals.However,a comprehensive understanding of priority areas from a multi-dimensional perspective is lacking,hindering effective conservation and development strategies.To address this,we developed a comprehensive assessment framework with a tailored indicator system,enabling a spatial evaluation of CA’s priority areas by integrating biodiversity,ecosystem services(ESs),and human activities.Combining zonation and geographical detectors,this approach facilitates spatial prioritization and examines ecological and socio-economic heterogeneity.Our findings reveal a heterogeneous distribution of priority areas across CA,with significant concentrations in eastern mountainous regions,river valleys,and oasis agricultural lands.We identified 184 key districts crucial for ecological and societal sustainability.Attribution analysis shows that natural factors like soil types,precipitation,and evapotranspiration significantly shape these areas,influencing human activities and the distribution of biodiversity and ESs.Multi-dimensional analysis indicates existing protected areas cover only 15%of the top 30%priority areas,revealing substantial conservation gaps.Additionally,a 38%overlap between ESs and human activities,along with 63.25%congruence in integrated areas,underscores significant human impacts on ecological systems and their dependency on ESs.Given CA’s limited resources,it is crucial to implement measures that strengthen conservation efforts,align ecological preservation with socio-economic demands,and enhance resource efficiency through sustainable integrated land and water resource management.
基金support from the National Natural Science Foundation of China(32172191)the Tianjin Research Innovation Project for Postgraduate Students(2021YJSB211).
文摘The physicochemical properties,structural characteristics,antioxidant,radioprotective and lipid-lowering activities,as well as the underlying structure-activity relationships of polysaccharides extracted from Nostoc flagelliforme grown under normal(WL-EPS-1),salt stress(NaCl-EPS-1)and mixotrophic culture conditions(Glu-EPS-1)were studied.The results demonstrated that WL-EPS-1,NaCl-EPS-1 and Glu-EPS-1 were heteropolysaccharides comprising different proportions of monosaccharides and uronic acid,with different average molecular weights of 0.99×10^(3),1.09×10^(3)and 1.18×10^(3)kDa,respectively.Their intrinsic viscosity were significantly different,at 24.72,29.98,and 41.06 dL/g,respectively.The functional groups of polysaccharides were not greatly affected,but the chemical composition,triple-helix structure,chain length and surface morphology were significantly influenced by culture conditions.In vitro bioactivity assays showed that the antioxidant activity generally increased in the order of WL-EPS-1<NaCl-EPS-1<Glu-EPS-1,while NaCl-EPS-1 had the best radioprotective effect,and Glu-EPS-1 had the best lipid-lowering effect.In addition,the structure-activity relationship of the polysaccharides was analyzed by partial least squares,which revealed that the most important factors affecting the antioxidant,radioprotective and lipid-lowering activities of polysaccharides were viscosity and molecular weight.This study provides a strategy for obtaining high-bioactivity polysaccharides by appropriate regulation of culture conditions,as well as opening new directions for the molecular modification of polysaccharides.
