Mucin 2(MUC2)is a critical component of the intestinal mucus barrier.Lactic acid bacteria(LAB)strains can improve mucosal homeostasis.In this study,we determined the expression of Muc2 induced by dead bacteria and cel...Mucin 2(MUC2)is a critical component of the intestinal mucus barrier.Lactic acid bacteria(LAB)strains can improve mucosal homeostasis.In this study,we determined the expression of Muc2 induced by dead bacteria and cell-free conditioned medium(CM)of 50 LAB strains in the human goblet cell line,LS174T.Dead bacteria or CM of LAB affected the Muc2 expression in a species-and strain-specific manner under homeostasis.Next,LAB strains with different regulatory abilities were selected,gavaged into mice,and exposed to dextran sodium sulfate(DSS)after 1 week.Different LAB strains inhibited intestinal injury to different degrees,with Lactobacillus acidophilus FCQHC4L1 exerting the most potent effect.FCQHC4L1 significantly decreased the secretion of pro-inflammatory factors,promoted the expression and secretion of mucin,and inhibited colitis development.This strain also regulated the gut microbiota and increased the secretion of butyric acid.Moreover,CM of FCQHC4L1 inhibited endoplasmic reticulum(ER)stress and ameliorated the abnormal expression of MUC2 by suppressing the activation of the GRP78/ATF6 and GRP78/IRE1/XBP1 signaling pathways.Our results highlight the potential of FCQHC4L1 as a therapeutic agent for strengthening the mucus barrier and improving the gut health.展开更多
Zinc-based alloys are promising biodegradable materials for application in the intestinal environment due to their appropriate degradation rates and favorable biocompatibility.However,the corrosion and degradation of ...Zinc-based alloys are promising biodegradable materials for application in the intestinal environment due to their appropriate degradation rates and favorable biocompatibility.However,the corrosion and degradation of biodegradable zinc alloys in the presence of intestinal microorganisms are seldom investigated.In this study,binary Zn-Mn alloys with 0.4 and 0.8 wt.%Mn content were fabricated using the extrusion process.The corrosion behaviors of pure zinc and Zn-Mn alloys with the existence of Lactobacillus acidophilus,a representative microorganism in the intestinal tract,were systematically investigated.In comparison to pure zinc,both Zn-Mn alloys exhibited enhanced strength and ductility.L.acidophilus significantly accelerated the corrosion of both pure zinc and Zn-Mn alloys by generating acidic agents.The presence of L.acidophilus increased the icorr values for pure zinc,Zn-0.4Mn,and Zn-0.8Mn from 68.7±9.9,33.9±2.3 and 17.1±0.1µA cm^(-2) to 253.5±26.7,167.6±8.7 and 30.6±2.2µA cm^(-2),respectively.The addition of Mn mitigated corrosion by refining grains and reducing the local surface potential difference.Compared to pure zinc,the surface potential difference of Zn-0.8Mn decreased from 31.8±1.7 mV to 11.8±0.9 mV.This study points out the existence of microbiologically influenced corrosion in the intestinal environment and emphasizes its importance in the comprehensive design of biodegradable zinc alloys.展开更多
Background: Clostridium perfringens is the main etiological agent of necrotic enteritis. Lactobacil i show beneficial effects on intestinal health in infectious disease, but the protective functions of lactobacil i in...Background: Clostridium perfringens is the main etiological agent of necrotic enteritis. Lactobacil i show beneficial effects on intestinal health in infectious disease, but the protective functions of lactobacil i in C. perfringens-infected chickens are scarcely described. This study examined the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) over a 28-day period. Using a 2 × 2 factorial arrangement of treatments, a total of 308 1-day-old male Arbor Acres broiler chicks were included to investigate the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) during a 28-day trial.Results: During infection(d 14–21), C. perfringens challenge decreased the average daily gain(P < 0.05), and increased feed conversion ratio and the mortality rate(P < 0.05). However, dietary supplementation with L. acidophilus increased the body weight of C. perfringens-infected broilers on d 21(P < 0.05), and tended to decrease the mortality(P = 0.061).C. perfringens challenge decreased the villus height(P < 0.05), the ratio of villus height to crypt depth(P < 0.05) and OCLN(occludin) m RNA expression(P < 0.05), and increased the pro-inflammatory cytokine expression in the spleen and jejunum, the intestinal populations of C. perfringens and Escherichia(P < 0.05), and the serum content of endotoxin(P < 0.05), regardless of L. acidophilus supplementation. In contrast, dietary L. acidophilus reducedthe intestinal lesion score of challenged broilers(P < 0.05), the m RNA expression of pro-inflammatory cytokines, ileal populations of Escherichia and serum endotoxin content(P < 0.05), but increased the intestinal Lactobacillus populations(P < 0.05),irrespective of C. perfringens challenge.Conclusion: Dietary addition of L. acidophilus could improve the intestinal health and reduce the mortality of broilers suffering from necrotic enteritis.展开更多
The aim of this study was to evaluate the effect of Lactobacillus acidophilus（LAC）, exogenous enzymes of ZAD（exogenous enzyme preparation） or their combination on feed conversion, and dressing of rabbits fed diffe...The aim of this study was to evaluate the effect of Lactobacillus acidophilus（LAC）, exogenous enzymes of ZAD（exogenous enzyme preparation） or their combination on feed conversion, and dressing of rabbits fed different treatments of sugarcane bagasse（SCB）. Five rations were allotted randomly to five groups of New-Zealand White（NZW） rabbits（n=10） with initial live body weight of（838±42.4） g and 5 weeks of age. Rabbits were fed on diets with different sources of fiber as follows,（i） a control diet composed of 100% berseem hay and 0% SCB,（ii） 50% berseem hay and 50% untreated SCB（USCB）,（iii） 50% berseem hay and 50% SCB treated with L. acidophilus（LAC）,（iv） 50% berseem hay and 50% SCB treated with ZAD（ZAD）, and（v） 50% berseem hay and 50% SCB treated with a combination of LAC＋ZAD（LZ）. Treatments of SCB with L. acidophilus, ZAD and LAC＋ZAD had the highest feed conversion ratio than both USCB and control. The dressing percentage of rabbits that fed the LAC and LZ diets was higher（P〈0.05） compared with that in the other groups. Performance index（PI） for LAC group was improved（P〈0.05） compared to that for the other groups; however, PI for USCB group was the lowest（P〈0.05）. It could be concluded that treating SCB with L. acidophilus, exogenous enzymes of ZAD or their combination improved feed conversion and performance with more positive effects with L. acidophilus than the other treatments.展开更多
AIM:To determine the efficacy profiles of different concentrations of Lactobacillus acidophilus(L.acidophilus)for treating colitis using an experimental murine model.METHODS:Colitis was established in 64 BALB/c mice b...AIM:To determine the efficacy profiles of different concentrations of Lactobacillus acidophilus(L.acidophilus)for treating colitis using an experimental murine model.METHODS:Colitis was established in 64 BALB/c mice by adding 5%dextran sodium sulfate(DSS)to the drinking water and allowing ad libitum access for 7 d.The mice were then randomly divided into the following control and experimental model groups(n=8 each;day 0):untreated model control;negative-treatment model control(administered gavage of 1 mL/10 g normal saline);experimental-treatment models C4-C8(administered gavage of 104,105,106,107,or 108CFU/10 g L.acidophilus,respectively);positive-treatment model control(administration of the anti-inflammatory agent prednisone acetate at 45 g/10 g).Eight mice given regular water(no DSS)and no subsequent treatments served as the normal control group.Body weight,fecal traits,and presence of fecal occult blood were assessed daily.All animals were sacrificed on post-treatment day7 to measure colonic length,perform histological scoring,and quantify the major bacteria in the proximal and distal colon.Intergroup differences were determined by one-way ANOVA and post-hoc Student-Newman-Keuls comparison.RESULTS:All treatments(L.acidophilus and prednisone acetate)protected against colitis-induced weight loss(P<0.05 vs model and normal control groups).The extent of colitis-induced colonic shortening was significantly reduced by all treatments(prednisone acetate>C4>C5>C7>C8>C6;P<0.05 vs untreated model group),and the C6 group showed colonic length similar to that of the normal control group(P>0.05).The C6 group also had the lowest disease activity index scores among the model groups.The bacterial profiles in the proximal colon were similar between all of the experimental-treatment model groups(all P>0.05).In contrast,the bacterial profile in the distal colon of the C6 group showed the distinctive features(P<0.05 vs all other experimental-treatment model groups)of Lactobacillus sp.and Bifidobacterium sp.being the most abundant bacteria and Staphylococcus aureus being the least abundant bacteria.CONCLUSION:The most therapeutically efficacious concentration of L.acidophilus(106CFU/10 g)may exert its effects by modulating the bacterial profile in the distal colon.展开更多
The species distinctive PCR primer of Lactobacillus acidophilus （ L. acidophilus） was designed according to 16S rRNA gene sequences of conunon Lac- tobacillus species in fermented material. Bacterial genome DNA of s...The species distinctive PCR primer of Lactobacillus acidophilus （ L. acidophilus） was designed according to 16S rRNA gene sequences of conunon Lac- tobacillus species in fermented material. Bacterial genome DNA of separated L. acidophilus in fermented sample was taken as template, and L. acidophilus in fer- mented material was conducted the quantitative determination by real-time quantitative PCR （RT-PCR）. Analysis on RT-PCR results shown that contents of L. aci- dophilus in the test sample reached 1.5 billion CFU / g. Test results shown that contents of L. acidophilus in fermented material could be detected accurately by the established RT-PCR method in the test. indicating that the established RT-PCR method could be aookued to the detection of L. acidophilus in fermented material.展开更多
Silver nanoparticles synthesized by dried biomass of Lactobaciluus acidophilus 01 strain was evaluated against toxicity of genomic DNA isolated from bacteria(E.coli) fungi(Beauveria bassiana) algae(Seenedesmus acutus)...Silver nanoparticles synthesized by dried biomass of Lactobaciluus acidophilus 01 strain was evaluated against toxicity of genomic DNA isolated from bacteria(E.coli) fungi(Beauveria bassiana) algae(Seenedesmus acutus) and human blood adopting standard condition was discussed in the present study.Synthesized silver nanoparticles were characterized by UV-Vis spectroscopy and SEM.The UV-Vis spectroscopy revealed the formation of silver nanoparticles by yielding the typical silver plasmon absorption maxima at 430 nm and SEM micrograph indicates the uniform spherical particles within the size range of 45?60 nm.The energy dispersive X-ray spectroscopy(EDX) of the nanoparticle confirmed the presence of elemental silver signal as strong peak.The above synthesized silver nanoparticles didn't cause any toxic effect on all the tested genomic DNA at all tested concentrations which reveals nil genomic nanoparticles induced toxicity.展开更多
Effects of diet fortified with Lactobacillus acidophilus at 0.0%, 0.2%, 0.4% and 0.6% on nonspecific immune response and disease resistant common carp juvenile infected with Pseudomonas aeruginosa and Aeromonas hydrop...Effects of diet fortified with Lactobacillus acidophilus at 0.0%, 0.2%, 0.4% and 0.6% on nonspecific immune response and disease resistant common carp juvenile infected with Pseudomonas aeruginosa and Aeromonas hydrophilla were examined. Immune response parameters （the total protein, albumin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, urea, creatinine and immunoglobulin）, percentage mortality and relative protection level were determined on day 1 and days 14, 28, 42 and 56. Fish fed Lactobacilus acidophilus fortified diet significantly improved nonspecific immune parameters especially group treated with 0.4% inclusion with lower mortality rate indicating a better relative protection level against Pseudomonas aeruginosa and Aeromonas hydrophilla.展开更多
Lactobacillus acidophilus belongs to the genus Lactobacillus, Gram-positive bacteria, which mainly colonize the small intestine of animals, can secrete anti-biotin substances, inhibit the growth and reproduction of so...Lactobacillus acidophilus belongs to the genus Lactobacillus, Gram-positive bacteria, which mainly colonize the small intestine of animals, can secrete anti-biotin substances, inhibit the growth and reproduction of some intestinal pathogenic bacteria, thereby regulating Intestinal flora, strengthen the absorption and utilization of feed nutrients, improve resistance, and promote animal growth, so it is widely used in livestock and poultry production. This article summarizes the latest research progress and application of Lactobacillus acidophilus, and looks forward to the future development direction in order to provide a theoretical basis for its future research.展开更多
The antagonistic activity of Lactobacillus acidophilus KLDS1.0901, KLDS1.0902, KLDSI.1003 and NCFM against Escherichia coli O157 ： H7 were investigated in this study. The culture supematants of all the L. acidophilus...The antagonistic activity of Lactobacillus acidophilus KLDS1.0901, KLDS1.0902, KLDSI.1003 and NCFM against Escherichia coli O157 ： H7 were investigated in this study. The culture supematants of all the L. acidophilus stains showed high bacteriostatic activities against E. coli O 157 ： H7 and the bacteriostatic substances of their Cell-Free Supernatants （CFS） were preliminarily determined from organic acids. The bacteriostatic activity from CFS or viable L. acidophilus against E. coli O157 ： H7 was also assessed by using co-incubation methods, CFS had high bactericidal activity against E. coli O157 ： H7, no viable E. coli O157 ： H7 was detected when 5×10^7 CfU ofE. coli O157 ： H7 was added to 5 mL of CFS and incubated at 37℃ for 2 h. However, L. acidophilus themselves had no bacteriostatic activity after directly contacted with E. coli O157 ： H7. The inhibition E. coli O157 ： H7 adhesion and colonization of L. acidophilus were also investigated based on competition, exclusion and displacement assays. L. acidophilus KLDS1.0901, KLDSI.1003 and NCFM strains were effective to displace E. coli O157 ： H7 from a Caco-2 cell layer in competition and exclusion assays. However, in displacement assay, all of the strains showed no significant antagonistic activities. Meanwhile, the probiotic potential of L. acidophilus strains was investigated based on adhesion assay to Caco-2 cells and anti- inflammatory effects by IL-8 produced in Caco-2 cells. The adhesion ability and anti-inflammatory effects of L. acidophilus strains showed a strain-dependent manner. In general, L. acidophilus KLDS 1.0901 and NCFM showed better probiotic potential than KLDS1.0902 and KLDSI.1003. Thus, the use ofL. acidophilus KLDS1.0901 and NCFM to prevent or treat of diseases associated induced E. coli O157 ： H7 in vivo was suggested.展开更多
Commercial interest in functional foods containing probiotic strains has consistently increased due to the awareness of gut health. Recent advancements are leading to development of synbiotic foods, containing prebiot...Commercial interest in functional foods containing probiotic strains has consistently increased due to the awareness of gut health. Recent advancements are leading to development of synbiotic foods, containing prebiotics and probiotics bearing synergistic effects of the two. Thus, in present study, synbiotic acido- philus milk was developed satisfying functional dairy food properties. Different sets of milk were fermented with probiotic cultures (Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus casei, bioyoghurt culture) singly or in combination, and prebiotics namely inulin (I), oat fibre (O) and honey (H). Obtained 20 synbiotic samples were organoleptically tested, physico-chemically (titrable acidity percentage (TA) &pH) and microbiologically (total viable count (TVC), coliform count and yeast &mold count) analyzed. The incorporation of honey and inulin led to development of sweetened and low calorie sweetened synbiotic acidophilus milk, respectively. Incorporation of B. bifidum increased the flavour of synbiotic acidophilus milk when compared to L. acidophilus as control, where as L. casei culture showed thinner consistency in the product. Addition of prebiotic affected only the sensory scores, whereas the probiotics addition resulted in a marginal variation of pH and TA. TVC of all synbiotic acidophilus milk samples obtained were more than desirable limits for harvesting probiotic effects (】10^10 cfu/ml). Finally, a two layer feed-forward artificial neural network (ANN) was established to predict the sensory evaluation based on inputs of probiotic and prebiotic.展开更多
Consu<span>mption of flaxseed provides health benefits. Bile tolerance allows </span>survival of probiotics in the intestinal tract. The objective was to determine whether or not flaxseed enhances bile tol...Consu<span>mption of flaxseed provides health benefits. Bile tolerance allows </span>survival of probiotics in the intestinal tract. The objective was to determine whether or not flaxseed enhances bile tolerance of </span><span style="font-family:""><i></span><i><span style="font-family:"">Lactobacillus acidophilus</span></i><span style="font-family:""> (<i>L. acidophilus</i></span><i><span style="font-family:""></i></span></i><span style="font-family:"">) LA-K<i>, </i></span><i><span style="font-family:""><i></span></i><i><span style="font-family:"">Lactobacillus delbruekii</span></i><i><span style="font-family:""></i></span></i><span style="font-family:""> ssp.<i> </i></span><i><span style="font-family:""><i></span></i><i><span style="font-family:"">bulgaricus </span></i><span style="font-family:"">(</span><i><span style="font-family:"">L. bulgaricus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:"">) LB-12,<span> </span></span><span style="font-family:"">and </span><span style="font-family:""><i></span><i><span style="font-family:"">Streptococcus salivarius</span></i><i><span style="font-family:""></i></span></i><i><span style="font-family:""> </span></i><span style="font-family:"">ssp.<i> </i></span><i><span style="font-family:""><i></span></i><i><span style="font-family:"">thermophilus </span></i><span style="font-family:"">(<i>S. </i></span><i><span style="font-family:"">thermophilus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:"">) ST-M5</span><i><span style="font-family:"">. </span></i><span style="font-family:"">Control and experimental (62 g flaxseed/L) broths containing 0.3% oxgall were prepared for each culture, sterilized, cooled, inoculated, and plated for 8 h. <span>Growth of each microorganism in both the control and </span>experimental broths was evaluated by the slope of the regression line of its log count versus time after inoculation. Flaxseed significantly enhanced growth of </span><span style="font-family:""><i></span><i><span style="font-family:"">L.</span></i><i><span style="font-family:""> acidophilus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:""> but not </span><span style="font-family:""><i></span><i><span style="font-family:"">L. </span></i><i><span style="font-family:"">bulgaricus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:""> and </span><span style="font-family:""><i></span><i><span style="font-family:"">S. thermophilus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:""> over 8 h compared to its corresponding control. Therefore, flaxseed improved the bile tolerance of </span><span style="font-family:""><i></span><i><span style="font-family:"">L. acidophilus</span></i><i><span style="font-family:""></i></span></i><i><span style="font-family:""> </span></i><span style="font-family:"">but not of </span><span style="font-family:""><i></span><i><span style="font-family:"">S. thermophilus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:""> and </span><span style="font-family:""><i></span><i><span style="font-family:"">L. bulgaricus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:"">.展开更多
AIM To assess the therapeutic potential of Lactobacillus acidophilus(LA) for the treatment of pouchitis in a rat model.METHODS Sprague Dawley rats underwent proctocolectomy and ileal pouch-anal anastomosis followed by...AIM To assess the therapeutic potential of Lactobacillus acidophilus(LA) for the treatment of pouchitis in a rat model.METHODS Sprague Dawley rats underwent proctocolectomy and ileal pouch-anal anastomosis followed by administration of dextran sulfate sodium(DSS) to induce pouchitis. Rats with pouchitis were randomly divided into three groups: no intervention(NI), normal saline(NS, 3 m L/d normal saline for 7 d), and LA(3 m L/d LA at 1× 1010 colony-forming units for 7 d). General body condition was recorded and pouch specimens were obtained for histological examination. m RNA expression levels of interleukin(IL)-1β, IL-6, IL-10, and tumor necrosis factor-α were determined by RT-PCR. Zonula occludens protein 1(ZO-1) levels were measured by immunohistochemistry. RESULTS LA reduced weight loss associated with pouchitis(P < 0.05) and improved the symptoms of pouchitis in rats. Compared with the NI and NS groups, rats in the LAgroup showed earlier disappearance of hematochezia(6.17 ± 0.75, 6.50 ± 0.55, 3.17 ± 0.75, P < 0.05) and higher fecal scores(2.67 ± 0.48, 2.50 ± 0.51, 4.42 ± 0.50, respectively, P < 0.05). Histological scores were also lower in the LA group compared with the other two groups(7.17 ± 0.98, 8.00 ± 0.89, 4.00 ± 0.89, respectively, P < 0.05). m RNA expression levels of IL-1β, IL-6, and tumor necrosis factor-α were significantly reduced, while IL-10 m RNA levels were significantly increased in the LA group(P < 0.05, respectively). ZO-1 protein levels were also significantly increased after administration of LA(P < 0.05). CONCLUSION LA alleviates pouchitis induced by DSS after ileal pouchanal anastomosis by decreasing pro-inflammatory factors and increasing anti-inflammatory factors, and restoring ZO-1 expression in the mucosa.展开更多
Lactobacillus acidophilus has several health benefits and is used as an adjunct bacterium in the manufacture of cultured dairy foods. Bile tolerance is an important probiotic characteristic. Pulsed electric field (PEF...Lactobacillus acidophilus has several health benefits and is used as an adjunct bacterium in the manufacture of cultured dairy foods. Bile tolerance is an important probiotic characteristic. Pulsed electric field (PEF) processing is non-thermal, hurdle technology which comprises of passing fluid foods between two electrodes and subjecting them to pulses of voltage for less than one second. Whether certain mild PEF conditions can enhance bile tolerance of Lactobacillus acidophilus is not known. The objective was to study the influence of certain PEF conditions on the, bile tolerance of Lactobacillus acidophilus LA-K. Lactobacillus acidophilus LA-K suspended in sterile peptone 0.1% w/v distilled water was treated with pulse widths of 3, 6 and 9 μs, pulse periods of 10,000;20,000 and 30,000 μs and electric field strengths of 5, 15 and 25 kV/cm. The control did not receive any pulsed electric field condition. Bile tolerance was determined hourly for 16 hours. PROC GLM of the Statistical Analysis Systems (SAS) was used for data analysis. Significant differences were determined at P p p p Lactobacillus acidophilus LA-K subjected to 5 kV/cm were signifycantly the highest while the bile tolerance when subjected to 25 kV/cm was significantly the lowest.展开更多
Pulsed electric field (PEF) processing involves the application of pulses of voltage for less than one second to fluid foods placed between two electrodes. Lactobacillus acidophilus is an important probiotic bacterium...Pulsed electric field (PEF) processing involves the application of pulses of voltage for less than one second to fluid foods placed between two electrodes. Lactobacillus acidophilus is an important probiotic bacterium used for the production of fermented dairy products. Acid tolerance is an important probiotic characteristic. The influence of PEF on acid tolerance of Lactobacillus acidophilus is not known. Objective of this study was to elucidate the influence of certain PEF conditions on the acid tolerance of Lactobacillus acidophilus LA-K. Freshly thawed Lactobacillus acidophilus LA-K was suspended in sterile peptone 0.1% w/v distilled water and treated in a pilot plant PEF system. The treatments were pulse width (3, 6 and 9 μs), pulse period (10,000;20,000 and 30,000 μs) and voltage (5, 15 and 25 kV/cm). Control was run through PEF system at 60 mL/min without receiving any pulsed electric field condition. Data were analyzed using the PROC GLM of the Statistical Analysis Systems (SAS). Differences of least square means were used to determine significant differences at P Lactobacillus acidophilus LA-K lowered by increasing pulse widths and voltages but lowering pulse periods.展开更多
<span><span>Greek yogurt has become much more popular within the last 15 to 20 years. The by-product of Greek yogurt manufacture is acid whey. Although acid whey has been considered a waste product, resear...<span><span>Greek yogurt has become much more popular within the last 15 to 20 years. The by-product of Greek yogurt manufacture is acid whey. Although acid whey has been considered a waste product, researchers are exploring various uses of this whey. Since the health benefits of consuming probiotics are widely known, one may propose adding probiotics to acid whey to form a probiotic beverage. Typically, probiotic bacteria do not thrive in acidic conditions. It would be beneficial to determine if the probiotic </span><i><span>Lactobacillus acidophilus </span></i><span>can survive in these acidic conditions. The objectives were to determine the growth of </span><i><span>L. acidophilus </span></i><span>in acid whey resulting from manufacturing Greek yogurt and to study any changes in apparent viscosity, pH, and titratable acidity over 4 weeks of refrigerated storage. Plain yogurt was manufactured, and whey was separated from plain yogurt to yield Greek yogurt and acid whey. Acid whey was batch pasteurized, cooled, sweetened, flavored with pineapple flavoring, inoculated with </span><i><span>L. acidophilus</span></i><span>, and stored at 4°C for 4 weeks. The log </span><i><span>L. acidophilus </span></i><span>counts progressively decreased from 7.84 immediately after manufacture to 2.06 at week 4. There were no significant changes in pH and titratable acidity of the pineapple-flavored probiotic acid whey over 4 weeks of storage, indicating product stability over shelf life. Viscosity changed over the storage time with minimum values at week 2 and maximum values at week 4. Although the counts declined over 4 weeks of storage, some </span><i><span>L. acidophilus </span></i><span>survived in the pineapple-flavored acid whey.</span></span>展开更多
Diabetic foot complications are very common and represent a serious health problem in Mexico because of their high frequency, high costs and difficulties in handling. The treatment of choice to inhibit bacteria relate...Diabetic foot complications are very common and represent a serious health problem in Mexico because of their high frequency, high costs and difficulties in handling. The treatment of choice to inhibit bacteria related to diabetic foot ulcer consists mainly of the use of cefotaxime however the problem with this treatment (antibiotics) is not always effective due to the pathophysiological condition of the patient, together with the resistance bacteria develop to the drugs. OMS has suggested the use of probiotics for research directed to the development of microbial interference therapies. This project used the Lyophilized conditioned medium with probiotics, extracellulars of probiotics, because there are reports in which wound healing in mice is observed employing probiotics. The objective of this study was to evaluate the biological activity of cefotaxime, clindamycin and thelyophilized conditioned media Lactobacillus acidophilus (LCMLa) on bacterias isolated from diabetic foot ulcer, this bioassay was performed by the turbidimetric method. The macroscopic analysis of the colonies was carried out and the morphological analysis of the bacteria was carried out using the atomic force microscope;in addition, the type of Gram and oxygen requirements for its growth were determined. From the diabetic foot ulcers, three strains were isolated, of which strain 1 and 3 whose morphology corresponds to a bacillus, was susceptible to cefotaxime and to the lyophilized conditioned medium of L. acidophilus. The potential of microbial interference that exhibits L. acidophilus on bacteria related to diabetic foot ulcer is demonstrated.展开更多
Objective: to analyze the effect of compound lactobacillus acidophilus tablet combined with omeprazole enteric-coated tablet on disease control in patients with acute gastroenteritis. Methods: patients with acute gast...Objective: to analyze the effect of compound lactobacillus acidophilus tablet combined with omeprazole enteric-coated tablet on disease control in patients with acute gastroenteritis. Methods: patients with acute gastroenteritis were selected as the subjects of the study, and the patients admitted from June 2019 to August 2020 were selected for the period of time. The patients were divided into two groups by random number method for comparative exploration, and the changes of the condition of 100 patients were recorded. The control group was given compound lactobacillus acidophilus tablet, and the observation group was given compound Lactobacillus acidophilus tablet combined with omeprazole enteric-coated tablet. The therapeutic effect was analyzed. Results: from the quality of treatment, the treatment effect of observation group was 90%, the control group was 78%, the difference between the groups was significant, with statistical significance (P < 0.05). At the same time, the incidence of adverse events in the observation group was 4% (2/50), significantly lower than that in the control group (10%, 5/50), and the difference was statistically significant. Conclusion: the combination of compound Lactobacillus acidophilus tablets and omeprazole enteric-coated tablets in the treatment of acute gastroenteritis can not only manage the disease and improve the therapeutic effect, but also have high safety guarantee and clinical promotion value.展开更多
基金funded by the Guangdong Province Key Research and Development Project(2022B111107006)the National Natural Science Foundation of China(32021005 and 31820103010)the Fundamental Research Funds for the Central Universities(JUSRP622013)。
文摘Mucin 2(MUC2)is a critical component of the intestinal mucus barrier.Lactic acid bacteria(LAB)strains can improve mucosal homeostasis.In this study,we determined the expression of Muc2 induced by dead bacteria and cell-free conditioned medium(CM)of 50 LAB strains in the human goblet cell line,LS174T.Dead bacteria or CM of LAB affected the Muc2 expression in a species-and strain-specific manner under homeostasis.Next,LAB strains with different regulatory abilities were selected,gavaged into mice,and exposed to dextran sodium sulfate(DSS)after 1 week.Different LAB strains inhibited intestinal injury to different degrees,with Lactobacillus acidophilus FCQHC4L1 exerting the most potent effect.FCQHC4L1 significantly decreased the secretion of pro-inflammatory factors,promoted the expression and secretion of mucin,and inhibited colitis development.This strain also regulated the gut microbiota and increased the secretion of butyric acid.Moreover,CM of FCQHC4L1 inhibited endoplasmic reticulum(ER)stress and ameliorated the abnormal expression of MUC2 by suppressing the activation of the GRP78/ATF6 and GRP78/IRE1/XBP1 signaling pathways.Our results highlight the potential of FCQHC4L1 as a therapeutic agent for strengthening the mucus barrier and improving the gut health.
基金financially supported by the National Natural Science Foundation of China(No.52231010).
文摘Zinc-based alloys are promising biodegradable materials for application in the intestinal environment due to their appropriate degradation rates and favorable biocompatibility.However,the corrosion and degradation of biodegradable zinc alloys in the presence of intestinal microorganisms are seldom investigated.In this study,binary Zn-Mn alloys with 0.4 and 0.8 wt.%Mn content were fabricated using the extrusion process.The corrosion behaviors of pure zinc and Zn-Mn alloys with the existence of Lactobacillus acidophilus,a representative microorganism in the intestinal tract,were systematically investigated.In comparison to pure zinc,both Zn-Mn alloys exhibited enhanced strength and ductility.L.acidophilus significantly accelerated the corrosion of both pure zinc and Zn-Mn alloys by generating acidic agents.The presence of L.acidophilus increased the icorr values for pure zinc,Zn-0.4Mn,and Zn-0.8Mn from 68.7±9.9,33.9±2.3 and 17.1±0.1µA cm^(-2) to 253.5±26.7,167.6±8.7 and 30.6±2.2µA cm^(-2),respectively.The addition of Mn mitigated corrosion by refining grains and reducing the local surface potential difference.Compared to pure zinc,the surface potential difference of Zn-0.8Mn decreased from 31.8±1.7 mV to 11.8±0.9 mV.This study points out the existence of microbiologically influenced corrosion in the intestinal environment and emphasizes its importance in the comprehensive design of biodegradable zinc alloys.
基金supported by Natural Science Foundation of China(No.3140130343)
文摘Background: Clostridium perfringens is the main etiological agent of necrotic enteritis. Lactobacil i show beneficial effects on intestinal health in infectious disease, but the protective functions of lactobacil i in C. perfringens-infected chickens are scarcely described. This study examined the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) over a 28-day period. Using a 2 × 2 factorial arrangement of treatments, a total of 308 1-day-old male Arbor Acres broiler chicks were included to investigate the effects of Lactobacil us acidophilus(L. acidophilus) on the growth performance and intestinal health of broiler chickens chal enged with Clostridium perfringens(C. perfringens) during a 28-day trial.Results: During infection(d 14–21), C. perfringens challenge decreased the average daily gain(P < 0.05), and increased feed conversion ratio and the mortality rate(P < 0.05). However, dietary supplementation with L. acidophilus increased the body weight of C. perfringens-infected broilers on d 21(P < 0.05), and tended to decrease the mortality(P = 0.061).C. perfringens challenge decreased the villus height(P < 0.05), the ratio of villus height to crypt depth(P < 0.05) and OCLN(occludin) m RNA expression(P < 0.05), and increased the pro-inflammatory cytokine expression in the spleen and jejunum, the intestinal populations of C. perfringens and Escherichia(P < 0.05), and the serum content of endotoxin(P < 0.05), regardless of L. acidophilus supplementation. In contrast, dietary L. acidophilus reducedthe intestinal lesion score of challenged broilers(P < 0.05), the m RNA expression of pro-inflammatory cytokines, ileal populations of Escherichia and serum endotoxin content(P < 0.05), but increased the intestinal Lactobacillus populations(P < 0.05),irrespective of C. perfringens challenge.Conclusion: Dietary addition of L. acidophilus could improve the intestinal health and reduce the mortality of broilers suffering from necrotic enteritis.
文摘The aim of this study was to evaluate the effect of Lactobacillus acidophilus（LAC）, exogenous enzymes of ZAD（exogenous enzyme preparation） or their combination on feed conversion, and dressing of rabbits fed different treatments of sugarcane bagasse（SCB）. Five rations were allotted randomly to five groups of New-Zealand White（NZW） rabbits（n=10） with initial live body weight of（838±42.4） g and 5 weeks of age. Rabbits were fed on diets with different sources of fiber as follows,（i） a control diet composed of 100% berseem hay and 0% SCB,（ii） 50% berseem hay and 50% untreated SCB（USCB）,（iii） 50% berseem hay and 50% SCB treated with L. acidophilus（LAC）,（iv） 50% berseem hay and 50% SCB treated with ZAD（ZAD）, and（v） 50% berseem hay and 50% SCB treated with a combination of LAC＋ZAD（LZ）. Treatments of SCB with L. acidophilus, ZAD and LAC＋ZAD had the highest feed conversion ratio than both USCB and control. The dressing percentage of rabbits that fed the LAC and LZ diets was higher（P〈0.05） compared with that in the other groups. Performance index（PI） for LAC group was improved（P〈0.05） compared to that for the other groups; however, PI for USCB group was the lowest（P〈0.05）. It could be concluded that treating SCB with L. acidophilus, exogenous enzymes of ZAD or their combination improved feed conversion and performance with more positive effects with L. acidophilus than the other treatments.
文摘AIM:To determine the efficacy profiles of different concentrations of Lactobacillus acidophilus(L.acidophilus)for treating colitis using an experimental murine model.METHODS:Colitis was established in 64 BALB/c mice by adding 5%dextran sodium sulfate(DSS)to the drinking water and allowing ad libitum access for 7 d.The mice were then randomly divided into the following control and experimental model groups(n=8 each;day 0):untreated model control;negative-treatment model control(administered gavage of 1 mL/10 g normal saline);experimental-treatment models C4-C8(administered gavage of 104,105,106,107,or 108CFU/10 g L.acidophilus,respectively);positive-treatment model control(administration of the anti-inflammatory agent prednisone acetate at 45 g/10 g).Eight mice given regular water(no DSS)and no subsequent treatments served as the normal control group.Body weight,fecal traits,and presence of fecal occult blood were assessed daily.All animals were sacrificed on post-treatment day7 to measure colonic length,perform histological scoring,and quantify the major bacteria in the proximal and distal colon.Intergroup differences were determined by one-way ANOVA and post-hoc Student-Newman-Keuls comparison.RESULTS:All treatments(L.acidophilus and prednisone acetate)protected against colitis-induced weight loss(P<0.05 vs model and normal control groups).The extent of colitis-induced colonic shortening was significantly reduced by all treatments(prednisone acetate>C4>C5>C7>C8>C6;P<0.05 vs untreated model group),and the C6 group showed colonic length similar to that of the normal control group(P>0.05).The C6 group also had the lowest disease activity index scores among the model groups.The bacterial profiles in the proximal colon were similar between all of the experimental-treatment model groups(all P>0.05).In contrast,the bacterial profile in the distal colon of the C6 group showed the distinctive features(P<0.05 vs all other experimental-treatment model groups)of Lactobacillus sp.and Bifidobacterium sp.being the most abundant bacteria and Staphylococcus aureus being the least abundant bacteria.CONCLUSION:The most therapeutically efficacious concentration of L.acidophilus(106CFU/10 g)may exert its effects by modulating the bacterial profile in the distal colon.
文摘The species distinctive PCR primer of Lactobacillus acidophilus （ L. acidophilus） was designed according to 16S rRNA gene sequences of conunon Lac- tobacillus species in fermented material. Bacterial genome DNA of separated L. acidophilus in fermented sample was taken as template, and L. acidophilus in fer- mented material was conducted the quantitative determination by real-time quantitative PCR （RT-PCR）. Analysis on RT-PCR results shown that contents of L. aci- dophilus in the test sample reached 1.5 billion CFU / g. Test results shown that contents of L. acidophilus in fermented material could be detected accurately by the established RT-PCR method in the test. indicating that the established RT-PCR method could be aookued to the detection of L. acidophilus in fermented material.
文摘Silver nanoparticles synthesized by dried biomass of Lactobaciluus acidophilus 01 strain was evaluated against toxicity of genomic DNA isolated from bacteria(E.coli) fungi(Beauveria bassiana) algae(Seenedesmus acutus) and human blood adopting standard condition was discussed in the present study.Synthesized silver nanoparticles were characterized by UV-Vis spectroscopy and SEM.The UV-Vis spectroscopy revealed the formation of silver nanoparticles by yielding the typical silver plasmon absorption maxima at 430 nm and SEM micrograph indicates the uniform spherical particles within the size range of 45?60 nm.The energy dispersive X-ray spectroscopy(EDX) of the nanoparticle confirmed the presence of elemental silver signal as strong peak.The above synthesized silver nanoparticles didn't cause any toxic effect on all the tested genomic DNA at all tested concentrations which reveals nil genomic nanoparticles induced toxicity.
基金Supported Partly by University of Ilorin Under University Staff Development Fund 2015
文摘Effects of diet fortified with Lactobacillus acidophilus at 0.0%, 0.2%, 0.4% and 0.6% on nonspecific immune response and disease resistant common carp juvenile infected with Pseudomonas aeruginosa and Aeromonas hydrophilla were examined. Immune response parameters （the total protein, albumin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, urea, creatinine and immunoglobulin）, percentage mortality and relative protection level were determined on day 1 and days 14, 28, 42 and 56. Fish fed Lactobacilus acidophilus fortified diet significantly improved nonspecific immune parameters especially group treated with 0.4% inclusion with lower mortality rate indicating a better relative protection level against Pseudomonas aeruginosa and Aeromonas hydrophilla.
文摘Lactobacillus acidophilus belongs to the genus Lactobacillus, Gram-positive bacteria, which mainly colonize the small intestine of animals, can secrete anti-biotin substances, inhibit the growth and reproduction of some intestinal pathogenic bacteria, thereby regulating Intestinal flora, strengthen the absorption and utilization of feed nutrients, improve resistance, and promote animal growth, so it is widely used in livestock and poultry production. This article summarizes the latest research progress and application of Lactobacillus acidophilus, and looks forward to the future development direction in order to provide a theoretical basis for its future research.
基金Supported by the Agro-scientific Research of China(201203009)the Ministry of Agriculture,China
文摘The antagonistic activity of Lactobacillus acidophilus KLDS1.0901, KLDS1.0902, KLDSI.1003 and NCFM against Escherichia coli O157 ： H7 were investigated in this study. The culture supematants of all the L. acidophilus stains showed high bacteriostatic activities against E. coli O 157 ： H7 and the bacteriostatic substances of their Cell-Free Supernatants （CFS） were preliminarily determined from organic acids. The bacteriostatic activity from CFS or viable L. acidophilus against E. coli O157 ： H7 was also assessed by using co-incubation methods, CFS had high bactericidal activity against E. coli O157 ： H7, no viable E. coli O157 ： H7 was detected when 5×10^7 CfU ofE. coli O157 ： H7 was added to 5 mL of CFS and incubated at 37℃ for 2 h. However, L. acidophilus themselves had no bacteriostatic activity after directly contacted with E. coli O157 ： H7. The inhibition E. coli O157 ： H7 adhesion and colonization of L. acidophilus were also investigated based on competition, exclusion and displacement assays. L. acidophilus KLDS1.0901, KLDSI.1003 and NCFM strains were effective to displace E. coli O157 ： H7 from a Caco-2 cell layer in competition and exclusion assays. However, in displacement assay, all of the strains showed no significant antagonistic activities. Meanwhile, the probiotic potential of L. acidophilus strains was investigated based on adhesion assay to Caco-2 cells and anti- inflammatory effects by IL-8 produced in Caco-2 cells. The adhesion ability and anti-inflammatory effects of L. acidophilus strains showed a strain-dependent manner. In general, L. acidophilus KLDS 1.0901 and NCFM showed better probiotic potential than KLDS1.0902 and KLDSI.1003. Thus, the use ofL. acidophilus KLDS1.0901 and NCFM to prevent or treat of diseases associated induced E. coli O157 ： H7 in vivo was suggested.
文摘Commercial interest in functional foods containing probiotic strains has consistently increased due to the awareness of gut health. Recent advancements are leading to development of synbiotic foods, containing prebiotics and probiotics bearing synergistic effects of the two. Thus, in present study, synbiotic acido- philus milk was developed satisfying functional dairy food properties. Different sets of milk were fermented with probiotic cultures (Lactobacillus acidophilus, Bifidobacterium bifidum, Lactobacillus casei, bioyoghurt culture) singly or in combination, and prebiotics namely inulin (I), oat fibre (O) and honey (H). Obtained 20 synbiotic samples were organoleptically tested, physico-chemically (titrable acidity percentage (TA) &pH) and microbiologically (total viable count (TVC), coliform count and yeast &mold count) analyzed. The incorporation of honey and inulin led to development of sweetened and low calorie sweetened synbiotic acidophilus milk, respectively. Incorporation of B. bifidum increased the flavour of synbiotic acidophilus milk when compared to L. acidophilus as control, where as L. casei culture showed thinner consistency in the product. Addition of prebiotic affected only the sensory scores, whereas the probiotics addition resulted in a marginal variation of pH and TA. TVC of all synbiotic acidophilus milk samples obtained were more than desirable limits for harvesting probiotic effects (】10^10 cfu/ml). Finally, a two layer feed-forward artificial neural network (ANN) was established to predict the sensory evaluation based on inputs of probiotic and prebiotic.
文摘Consu<span>mption of flaxseed provides health benefits. Bile tolerance allows </span>survival of probiotics in the intestinal tract. The objective was to determine whether or not flaxseed enhances bile tolerance of </span><span style="font-family:""><i></span><i><span style="font-family:"">Lactobacillus acidophilus</span></i><span style="font-family:""> (<i>L. acidophilus</i></span><i><span style="font-family:""></i></span></i><span style="font-family:"">) LA-K<i>, </i></span><i><span style="font-family:""><i></span></i><i><span style="font-family:"">Lactobacillus delbruekii</span></i><i><span style="font-family:""></i></span></i><span style="font-family:""> ssp.<i> </i></span><i><span style="font-family:""><i></span></i><i><span style="font-family:"">bulgaricus </span></i><span style="font-family:"">(</span><i><span style="font-family:"">L. bulgaricus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:"">) LB-12,<span> </span></span><span style="font-family:"">and </span><span style="font-family:""><i></span><i><span style="font-family:"">Streptococcus salivarius</span></i><i><span style="font-family:""></i></span></i><i><span style="font-family:""> </span></i><span style="font-family:"">ssp.<i> </i></span><i><span style="font-family:""><i></span></i><i><span style="font-family:"">thermophilus </span></i><span style="font-family:"">(<i>S. </i></span><i><span style="font-family:"">thermophilus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:"">) ST-M5</span><i><span style="font-family:"">. </span></i><span style="font-family:"">Control and experimental (62 g flaxseed/L) broths containing 0.3% oxgall were prepared for each culture, sterilized, cooled, inoculated, and plated for 8 h. <span>Growth of each microorganism in both the control and </span>experimental broths was evaluated by the slope of the regression line of its log count versus time after inoculation. Flaxseed significantly enhanced growth of </span><span style="font-family:""><i></span><i><span style="font-family:"">L.</span></i><i><span style="font-family:""> acidophilus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:""> but not </span><span style="font-family:""><i></span><i><span style="font-family:"">L. </span></i><i><span style="font-family:"">bulgaricus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:""> and </span><span style="font-family:""><i></span><i><span style="font-family:"">S. thermophilus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:""> over 8 h compared to its corresponding control. Therefore, flaxseed improved the bile tolerance of </span><span style="font-family:""><i></span><i><span style="font-family:"">L. acidophilus</span></i><i><span style="font-family:""></i></span></i><i><span style="font-family:""> </span></i><span style="font-family:"">but not of </span><span style="font-family:""><i></span><i><span style="font-family:"">S. thermophilus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:""> and </span><span style="font-family:""><i></span><i><span style="font-family:"">L. bulgaricus</span></i><i><span style="font-family:""></i></span></i><span style="font-family:"">.
基金Supported by Jie-Shou Li Gut Barrier Foundation,No.LJS_201008
文摘AIM To assess the therapeutic potential of Lactobacillus acidophilus(LA) for the treatment of pouchitis in a rat model.METHODS Sprague Dawley rats underwent proctocolectomy and ileal pouch-anal anastomosis followed by administration of dextran sulfate sodium(DSS) to induce pouchitis. Rats with pouchitis were randomly divided into three groups: no intervention(NI), normal saline(NS, 3 m L/d normal saline for 7 d), and LA(3 m L/d LA at 1× 1010 colony-forming units for 7 d). General body condition was recorded and pouch specimens were obtained for histological examination. m RNA expression levels of interleukin(IL)-1β, IL-6, IL-10, and tumor necrosis factor-α were determined by RT-PCR. Zonula occludens protein 1(ZO-1) levels were measured by immunohistochemistry. RESULTS LA reduced weight loss associated with pouchitis(P < 0.05) and improved the symptoms of pouchitis in rats. Compared with the NI and NS groups, rats in the LAgroup showed earlier disappearance of hematochezia(6.17 ± 0.75, 6.50 ± 0.55, 3.17 ± 0.75, P < 0.05) and higher fecal scores(2.67 ± 0.48, 2.50 ± 0.51, 4.42 ± 0.50, respectively, P < 0.05). Histological scores were also lower in the LA group compared with the other two groups(7.17 ± 0.98, 8.00 ± 0.89, 4.00 ± 0.89, respectively, P < 0.05). m RNA expression levels of IL-1β, IL-6, and tumor necrosis factor-α were significantly reduced, while IL-10 m RNA levels were significantly increased in the LA group(P < 0.05, respectively). ZO-1 protein levels were also significantly increased after administration of LA(P < 0.05). CONCLUSION LA alleviates pouchitis induced by DSS after ileal pouchanal anastomosis by decreasing pro-inflammatory factors and increasing anti-inflammatory factors, and restoring ZO-1 expression in the mucosa.
文摘Lactobacillus acidophilus has several health benefits and is used as an adjunct bacterium in the manufacture of cultured dairy foods. Bile tolerance is an important probiotic characteristic. Pulsed electric field (PEF) processing is non-thermal, hurdle technology which comprises of passing fluid foods between two electrodes and subjecting them to pulses of voltage for less than one second. Whether certain mild PEF conditions can enhance bile tolerance of Lactobacillus acidophilus is not known. The objective was to study the influence of certain PEF conditions on the, bile tolerance of Lactobacillus acidophilus LA-K. Lactobacillus acidophilus LA-K suspended in sterile peptone 0.1% w/v distilled water was treated with pulse widths of 3, 6 and 9 μs, pulse periods of 10,000;20,000 and 30,000 μs and electric field strengths of 5, 15 and 25 kV/cm. The control did not receive any pulsed electric field condition. Bile tolerance was determined hourly for 16 hours. PROC GLM of the Statistical Analysis Systems (SAS) was used for data analysis. Significant differences were determined at P p p p Lactobacillus acidophilus LA-K subjected to 5 kV/cm were signifycantly the highest while the bile tolerance when subjected to 25 kV/cm was significantly the lowest.
文摘Pulsed electric field (PEF) processing involves the application of pulses of voltage for less than one second to fluid foods placed between two electrodes. Lactobacillus acidophilus is an important probiotic bacterium used for the production of fermented dairy products. Acid tolerance is an important probiotic characteristic. The influence of PEF on acid tolerance of Lactobacillus acidophilus is not known. Objective of this study was to elucidate the influence of certain PEF conditions on the acid tolerance of Lactobacillus acidophilus LA-K. Freshly thawed Lactobacillus acidophilus LA-K was suspended in sterile peptone 0.1% w/v distilled water and treated in a pilot plant PEF system. The treatments were pulse width (3, 6 and 9 μs), pulse period (10,000;20,000 and 30,000 μs) and voltage (5, 15 and 25 kV/cm). Control was run through PEF system at 60 mL/min without receiving any pulsed electric field condition. Data were analyzed using the PROC GLM of the Statistical Analysis Systems (SAS). Differences of least square means were used to determine significant differences at P Lactobacillus acidophilus LA-K lowered by increasing pulse widths and voltages but lowering pulse periods.
文摘<span><span>Greek yogurt has become much more popular within the last 15 to 20 years. The by-product of Greek yogurt manufacture is acid whey. Although acid whey has been considered a waste product, researchers are exploring various uses of this whey. Since the health benefits of consuming probiotics are widely known, one may propose adding probiotics to acid whey to form a probiotic beverage. Typically, probiotic bacteria do not thrive in acidic conditions. It would be beneficial to determine if the probiotic </span><i><span>Lactobacillus acidophilus </span></i><span>can survive in these acidic conditions. The objectives were to determine the growth of </span><i><span>L. acidophilus </span></i><span>in acid whey resulting from manufacturing Greek yogurt and to study any changes in apparent viscosity, pH, and titratable acidity over 4 weeks of refrigerated storage. Plain yogurt was manufactured, and whey was separated from plain yogurt to yield Greek yogurt and acid whey. Acid whey was batch pasteurized, cooled, sweetened, flavored with pineapple flavoring, inoculated with </span><i><span>L. acidophilus</span></i><span>, and stored at 4°C for 4 weeks. The log </span><i><span>L. acidophilus </span></i><span>counts progressively decreased from 7.84 immediately after manufacture to 2.06 at week 4. There were no significant changes in pH and titratable acidity of the pineapple-flavored probiotic acid whey over 4 weeks of storage, indicating product stability over shelf life. Viscosity changed over the storage time with minimum values at week 2 and maximum values at week 4. Although the counts declined over 4 weeks of storage, some </span><i><span>L. acidophilus </span></i><span>survived in the pineapple-flavored acid whey.</span></span>
文摘Diabetic foot complications are very common and represent a serious health problem in Mexico because of their high frequency, high costs and difficulties in handling. The treatment of choice to inhibit bacteria related to diabetic foot ulcer consists mainly of the use of cefotaxime however the problem with this treatment (antibiotics) is not always effective due to the pathophysiological condition of the patient, together with the resistance bacteria develop to the drugs. OMS has suggested the use of probiotics for research directed to the development of microbial interference therapies. This project used the Lyophilized conditioned medium with probiotics, extracellulars of probiotics, because there are reports in which wound healing in mice is observed employing probiotics. The objective of this study was to evaluate the biological activity of cefotaxime, clindamycin and thelyophilized conditioned media Lactobacillus acidophilus (LCMLa) on bacterias isolated from diabetic foot ulcer, this bioassay was performed by the turbidimetric method. The macroscopic analysis of the colonies was carried out and the morphological analysis of the bacteria was carried out using the atomic force microscope;in addition, the type of Gram and oxygen requirements for its growth were determined. From the diabetic foot ulcers, three strains were isolated, of which strain 1 and 3 whose morphology corresponds to a bacillus, was susceptible to cefotaxime and to the lyophilized conditioned medium of L. acidophilus. The potential of microbial interference that exhibits L. acidophilus on bacteria related to diabetic foot ulcer is demonstrated.
文摘Objective: to analyze the effect of compound lactobacillus acidophilus tablet combined with omeprazole enteric-coated tablet on disease control in patients with acute gastroenteritis. Methods: patients with acute gastroenteritis were selected as the subjects of the study, and the patients admitted from June 2019 to August 2020 were selected for the period of time. The patients were divided into two groups by random number method for comparative exploration, and the changes of the condition of 100 patients were recorded. The control group was given compound lactobacillus acidophilus tablet, and the observation group was given compound Lactobacillus acidophilus tablet combined with omeprazole enteric-coated tablet. The therapeutic effect was analyzed. Results: from the quality of treatment, the treatment effect of observation group was 90%, the control group was 78%, the difference between the groups was significant, with statistical significance (P < 0.05). At the same time, the incidence of adverse events in the observation group was 4% (2/50), significantly lower than that in the control group (10%, 5/50), and the difference was statistically significant. Conclusion: the combination of compound Lactobacillus acidophilus tablets and omeprazole enteric-coated tablets in the treatment of acute gastroenteritis can not only manage the disease and improve the therapeutic effect, but also have high safety guarantee and clinical promotion value.
