Short-chain fatty acids,metabolites produced by the fermentation of dietary fiber by gut microbiota,have garnered significant attention due to their correlation with neurodegenerative diseases,particularly Parkinson’...Short-chain fatty acids,metabolites produced by the fermentation of dietary fiber by gut microbiota,have garnered significant attention due to their correlation with neurodegenerative diseases,particularly Parkinson’s disease.In this review,we summarize the changes in short-chain fatty acid levels and the abundance of short-chain fatty acid-producing bacteria in various samples from patients with Parkinson’s disease,highlighting the critical role of gut homeostasis imbalance in the pathogenesis and progression of the disease.Focusing on the nervous system,we discuss the molecular mechanisms by which short-chain fatty acids influence the homeostasis of both the enteric nervous system and the central nervous system.We identify key processes,including the activation of G protein-coupled receptors and the inhibition of histone deacetylases by short-chain fatty acids.Importantly,structural or functional disruptions in the enteric nervous system mediated by these fatty acids may lead to abnormalα-synuclein expression and gastrointestinal dysmotility,which could serve as an initiating event in Parkinson’s disease.Furthermore,we propose that short-chain fatty acids help establish communication between the enteric nervous system and the central nervous system via the vagal nerve,immune circulation,and endocrine signaling.This communication may shed light on their potential role in the transmission ofα-synuclein from the gut to the brain.Finally,we elucidate novel treatment strategies for Parkinson’s disease that target short-chain fatty acids and examine the challenges associated with translating short-chain fatty acid-based therapies into clinical practice.In conclusion,this review emphasizes the pivotal role of short-chain fatty acids in regulating gut-brain axis integrity and their significance in the pathogenesis of Parkinson’s disease from the perspective of the nervous system.Moreover,it highlights the potential value of short-chain fatty acids in early intervention for Parkinson’s disease.Future research into the molecular mechanisms of short-chain fatty acids and their synergistic interactions with other gut metabolites is likely to advance the clinical translation of innovative short-chain fatty acid-based therapies for Parkinson’s disease.展开更多
Three copper(Ⅱ),nickel and cadmium(Ⅱ)complexes,namely[Cu_(2)(μ-H2dbda)2(phen)2]·2H_(2)O(1),[Ni(μ-H2dbda)(μ-bpb)(H_(2)O)2]n(2),and[Cd(μ-H2dbda)(μ-bpa)]n(3),have been constructed hydrothermally using H4dbda(...Three copper(Ⅱ),nickel and cadmium(Ⅱ)complexes,namely[Cu_(2)(μ-H2dbda)2(phen)2]·2H_(2)O(1),[Ni(μ-H2dbda)(μ-bpb)(H_(2)O)2]n(2),and[Cd(μ-H2dbda)(μ-bpa)]n(3),have been constructed hydrothermally using H4dbda(4,4'-dihydroxy-[1,1'-biphenyl]-3,3'-dicarboxylic acid),phen(1,10-phenanthroline),bpb(1,4-bis(pyrid-4-yl)benzene),bpa(bis(4-pyridyl)amine),and copper,nickel and cadmium chlorides at 160℃.The products were isolated as stable crystalline solids and were characterized by IR spectra,elemental analyses,thermogravimetric analyses,and singlecrystal X-ray diffraction analyses.Single-crystal X-ray diffraction analyses revealed that three complexes crystallize in the monoclinic P21/n,tetragonal I42d,and orthorhombic P21212 space groups.The complexes exhibit molecular dimers(1)or 2D metal-organic networks(2 and 3).The catalytic performances in the Knoevenagel reaction of these complexes were investigated.Complex 1 exhibits an effective catalytic activity and excellent reusability as a heterogeneous catalyst in the Knoevenagel reaction at room temperature.CCDC:2463800,1;2463801,2;2463802,3.展开更多
Methane dehydroaromatization(MDA)presents a promising carbon-neutral pathway for benzene,toluene,and xylene(BTX)production,alternative to petroleumderived routes.Elucidating the regulatory mechanisms of Brønsted ...Methane dehydroaromatization(MDA)presents a promising carbon-neutral pathway for benzene,toluene,and xylene(BTX)production,alternative to petroleumderived routes.Elucidating the regulatory mechanisms of Brønsted acid site(BAS)strength on reaction pathways,alongside the spatial proximity effects between BAS and Mo active sites in bifunctional synergy,remains a critical scientific challenge in catalyst design.This study systematically tunes both BAS strength(via isomorphous metal substitution)and Mo-BAS spatial proximity in zeolites,integrating MDA catalytic evaluations with density functional theory(DFT)calculations to dissect their individual contributions.Strongly acidic BAS catalysts(compared to moderately acidic Fe/Ga-substituted counterparts)exhibit superior performance,evidenced by enhanced aromatic yields.Conversely,weakly acidic Bsubstituted zeolites demonstrate optimal mono-/bifunctional synergy,outperforming moderate-acid systems.DFT results reveal that acid strength dictates C−H activation mechanisms by modulating the energy barriers of rate-determining steps.While Al-zeolites deliver the highest activity,B-substituted systems display unique potential for mechanistic investigations.Spatial proximity analysis indicates that micrometer-scale Mo-BAS distances hinder effective synergy due to exceeding electron interaction and mass transfer limits,whereas nanometer-scale proximity enhances activity(via accelerated intermediate transport)and suppresses coke formation.These findings establish a theoretical framework for rationalizing zeolite catalyst optimization through BAS property engineering and spatial control of Mo-BAS cooperation,providing actionable guidelines for designing next-generation MDA catalysts.展开更多
This study employed integrated multi-omics approaches to elucidate,from the perspective of amino acid metabolism,the adaptive mechanism of Penicillium digitatum under modified atmosphere packaging(MAP)conditions.Compa...This study employed integrated multi-omics approaches to elucidate,from the perspective of amino acid metabolism,the adaptive mechanism of Penicillium digitatum under modified atmosphere packaging(MAP)conditions.Comparative analysis of natural air(Air),controlled atmosphere(CA),and MAP treatments revealed that MAP upregulated the expression of the hercynylcysteine S-oxide synthase(HCSOS),aldehyde dehydrogenase(ALDH),and monoamine oxidase(MAO)genes,thereby enhancing histidine-derived ergothioneine and methionine levels,and subsequently boosting glutathione-mediated redox homeostasis.Meanwhile,MAP induced the expression of the dihydroxyacid dehydratase(DHAD),saccharopine dehydrogenase(SDH),and arginosuccinate lyase(ASL)genes,redirecting valine,lysine,and arginine into the tricarboxylic acid(TCA)cycle to fuel ATP production.MAP also enhanced ASL-mediated arginine degradation and urea cycle activity,reducing arginine accumulation when compared to CA treatment.In contrast,while MAP induced upregulated expression of the pyrroline-5-carboxylate dehydrogenase(P5CDH)and D-amino acid oxidase(DAAO)genes,CA treatment promoted proline accumulation,reflecting stress-specific metabolic flexibility.Collectively,these findings demonstrate that MAP triggers transcriptional reprogramming of amino acid metabolism to coordinate oxidative defense,energy generation,and osmotic balance.By modulating these metabolic pathways and regulatory genes under MAP conditions,fungal adaptability can be disrupted.Hence,this study provides a promising strategy for suppressing green mold development,extending the postharvest shelf life,and improving the quality of fruits and vegetables.展开更多
The efficient extraction and separation of valuable metal elements from coal gasification fine slag(CGFS)are crucial for the comprehensive high-value utilization of its constituents.This study focused on the carbon-ri...The efficient extraction and separation of valuable metal elements from coal gasification fine slag(CGFS)are crucial for the comprehensive high-value utilization of its constituents.This study focused on the carbon-rich components of CGFS(CGFS-H)and systematically investigates the selective leaching behavior of Fe^(3+),Al^(3+)and Ca^(2+)using three organic acid extractants,i.e.,citric acid,tartaric acid,and tetrasodium iminodisuccinate.Additionally,the stepwise leaching of iron,aluminum and calcium from CGFS-H is explored.The selective dissolution mechanisms of these metals by different organic acids are elucidated through X-ray diffraction(XRD),X-ray fluorescence(XRF),and scanning electron microscopy(SEM)analyses.The results indicate that tetrasodium iminodisuccinate exhibits the highest leaching selectivity for Fe^(3+),while tartaric acid demonstrateds a comparable affinity for both Fe^(3+)and Al^(3+).In contrast citric acid shows superior selectivity toward Ca^(2+).The leaching yield of Fe^(3+),Al^(3+)and Ca^(2+)after sequential leaching with the three organic acids were 79.8%,65.08%and 78.6%,respectively.These findings confirm that effective and selective separation of Fe^(3+),Al^(3+)and Ca^(2+)from CGFS-H can be achieved via optimized organic acid-based leaching strategies.This advancement provides a critical foundation for developing Ca/Fe/Al hydrotalcite materials using CGFS-H as a sustainable feedstock,thereby facilitating the transformation of waste residue into high-value functional materials and promoting resourceefficient utilization of coal gasification fine slag.展开更多
The hydrated tricyclohexyltin theophylline-7-acetic acid(tpH)complex[Sn(C_(6)H_(11))_(3)(tp)(H_(2)O)]was synthesized via an ethanol solvothermal method using tricyclohexyltin hydroxide and tpH in a 1∶1 molar ratio.Th...The hydrated tricyclohexyltin theophylline-7-acetic acid(tpH)complex[Sn(C_(6)H_(11))_(3)(tp)(H_(2)O)]was synthesized via an ethanol solvothermal method using tricyclohexyltin hydroxide and tpH in a 1∶1 molar ratio.The complex was characterized by IR,^(1)H(^(13)C)NMR,elemental analysis,and powder X-ray diffraction,and the crystal structure was determined by single-crystal X-ray diffraction.The crystal belongs to the orthorhombic system with space group Iba2,and the central tin atom is in a five-coordinated trigonal bipyramidal configuration.Quantum chemistry ab initio calculations were performed to investigate the stability,molecular orbital energy,and frontier molecular orbital characteristics of the complex.Additionally,its thermal stability,electrochemical properties,and in vitro anticancer activity were evaluated.CCDC:2380308.展开更多
Background:This study aimed to comprehensively elucidate the hepatotoxic effects of Dioscorea bulbifera L.(DBL)in mice and to explore the underlying molecular mechanisms.Methods:Mice were administered different doses ...Background:This study aimed to comprehensively elucidate the hepatotoxic effects of Dioscorea bulbifera L.(DBL)in mice and to explore the underlying molecular mechanisms.Methods:Mice were administered different doses of DBL via intragastric gavage for 21 days.Organ indices of multiple organs were then measured,and histopathological changes were systematically evaluated.Particular attention was paid to biochemical markers and hepatic histopathological alterations associated with liver injury.Subsequently,the composition of the gut microbiota in colonic contents was analyzed using 16S rDNA sequencing,and the levels of short-chain fatty acids(SCFAs)were determined by gas chromatography(GC).Furthermore,protein expression levels of YinYang 1(YY1),Farnesoid X Receptor(FXR),Nuclear factor erythroid 2-related factor 2(Nrf2),and bile acids(BAs)-related proteins in the liver were assessed by Western blot analysis.Results:Our findings revealed that DBL induced varying degrees of multi-organ toxicity in mice,with the most pronounced hepatotoxicity observed.Liver injury was induced in a dose-dependent manner,accompanied by increased hepatic inflammation and oxidative stress,as well as enhanced hepatocyte apoptosis.Furthermore,DBL may modulate the YY1-FXR/Nrf2 signaling pathway,leading to upregulation of BAs-related proteins such as cholesterol 7α-hydroxylase(CYP7A1),while downregulating other BAs-related proteins,including sodium taurocholate cotransporting polypeptide(NTCP),bile salt export pump(BSEP),multidrug resistance protein 2(MRP2),and P-glycoprotein(P-gp).These changes promoted excessive BAs synthesis and impaired the excretion and reabsorption of BAs,ultimately disturbing BAs homeostasis.Additionally,DBL induces gut microbiota dysbiosis and reduces SCFAs production,leading to intestinal damage and disruption of intestinal barrier integrity.Conclusion:DBL-induced hepatotoxicity may be associated with altered expression of the YY1-FXR/Nrf2 signaling pathway and BAs–related proteins,accompanied by increased hepatic BAs accumulation and gut microbiota dysbiosis.展开更多
Both linoleic acid(18:2 n-6,LA)andα-linolenic acid(18:3 n-3,ALA)are essential fatty acids for infants.The contents of LA and ALA,and their ratio exhibited significant changes in human milk over the past 4 decades,whi...Both linoleic acid(18:2 n-6,LA)andα-linolenic acid(18:3 n-3,ALA)are essential fatty acids for infants.The contents of LA and ALA,and their ratio exhibited significant changes in human milk over the past 4 decades,which were not well summarized.Here,we summarized these values in 9898 human breast milk samples of 6664 mothers from 50 countries in 81 studies.A literature search was conducted using PubMed,Embase,and Web of Science between January 1980 and October 2023.The 95%confidence interval of LA/ALA ratio across lactation and gestation ranged from 14.24 to 31.26.The LA content was higher in China and Turkey(>20%)whereas the ALA content was below 1%in Africa.The LA/ALA ratio in countries along the Mediterranean coast exceeded 20 or even 30.LA and ALA contents increased significantly(P<0.01)while the ratio remained stable over the last 40 years.Multivariate meta-regression results showed that regions significantly(P<0.01)determined the LA,ALA,and LA/ALA ratio.Especially,maternal diet could definitely explain the variation while the effects of gestational age,lactation period was not significant.Clinical trials demonstrated that decreasing the LA/ALA ratio increased docosahexaenoic acid(22:6 n-3,DHA)status,reduced arachidonic acid(20:4 n-6,AA)contents,exerted no effect on the visual function of infants,and reached no consensus on growth.The current review aims to provide an overview on the LA and ALA contents and their ratio in human breast milk to raise concern in infant formula.展开更多
Background Ruminants and monogastric animals exhibit significant differences in gluconeogenic efficiency.In dairy cows,hepatic gluconeogenesis serves as the primary source of glucose.Metabolites modulate gluconeogenes...Background Ruminants and monogastric animals exhibit significant differences in gluconeogenic efficiency.In dairy cows,hepatic gluconeogenesis serves as the primary source of glucose.Metabolites modulate gluconeogenesis efficiency through allosteric regulation,redox state,and signal transduction pathways.However,the liver-enriched metabolites that regulate hepatic gluconeogenesis in dairy cows and their specific regulatory mechanisms remain incompletely characterized.Results Six Holstein dairy cows and six Duroc×(Landrace×Yorkshire)(DLY)crossbred pigs served as research subjects.Employing non-targeted and targeted metabolomics,we discovered that three bile acids—taurodeoxycholic acid(TDCA),taurocholic acid(TCA),and glycocholic acid(GCA)—were highly enriched in Holstein dairy cows'livers.In bovine hepatocytes,individual or combined stimulation of these bile acids significantly upregulated the expression of gluconeogenesis genes(FBP1,PCK1 and G6PC)and enhanced glucose production.In fasting mice with induced gluconeogenesis,TDCA,TCA,and GCA increased fasting blood glucose levels,and pyruvate tolerance tests further revealed their capacity to enhance hepatic gluconeogenesis,enabling more efficient glucose synthesis from pyruvate.Mechanistically,these bile acids activated Takeda G protein-coupled receptor 5(TGR5),elevated intracellular cAMP levels,and ultimately enhanced gluconeogenesis via the transcription factor cAMP-response element binding protein(CREB).Notably,a TGR5 inhibitor abrogated the stimulatory effects of TDCA,TCA,and GCA on hepatic gluconeogenesis in fasting mice.Conclusion TDCA,TCA,and GCA are key metabolites promoting hepatic gluconeogenesis in dairy cows,with TGR5 as the pivotal receptor and the cAMP/PKA/CREB pathway as the critical downstream mechanism.展开更多
In previous research,we demonstrated that long-term consumption of thermally oxidized oil leads to neuroinflammation and anxiety in mice.Therefore,in this study,we employed polar lipid components from thermo-induced o...In previous research,we demonstrated that long-term consumption of thermally oxidized oil leads to neuroinflammation and anxiety in mice.Therefore,in this study,we employed polar lipid components from thermo-induced oxidized oil to induce neurodamage.Behavioral assessments revealed that both the linoleic acid and AUDA(a classical inhibitor of soluble epoxide hydrolase)groups exhibited significantly reduced anxiety-like behaviors compared to the model group(P<0.05).Immunofluorescence analysis indicated that microglial activation in the hippocampus was attenuated in both the linoleic acid and AUDA groups relative to the model group,accompanied by a reduction in the m RNA expression of pro-inflammatory cytokines(IL-1β,IL-6,NOS2,TNF-α)and an upregulation of neuroprotective factors(IL-4,IL-10,BDNF).Lipidomic profiling of hippocampal tissue revealed that the lipid composition of the linoleic acid group closely resembled that of the AUDA group,with a significant downregulation of cardiolipin(CL)compared to the control group,consistent with alterations in the membrane potential channel receptor TRPC1.Both linoleic acid and AUDA inhibited the m RNA expression of EPHX2,leading to an increase in epoxyeicosatrienoic acids(EETs)levels.Furthermore,linoleic acid upregulated the expression of cytochrome P450 enzymes(CYP2J6)and lipoxygenase(LOX2S),which further upregulated the synthesis of EETs,and increased the content of 9-HODE and 13-HODE.These findings collectively suggest that linoleic acid alleviates neuroinflammation by modulating microglial differentiation and attenuates neurodegeneration induced by thermally oxidized oil through the regulation of arachidonic acid metabolism and the linoleic acid metabolic pathway,leading to the production of neuroprotective lipid mediators.Therefore,linoleic acid may serve as a potential neuro-nutrient for the treatment of anxiety disorders.This provided a scientific basis for the development of specialized medical foods aimed at protecting neural health.展开更多
Although amide-and hydrazide-based nucleating agents have been extensively used to enhance the crystallization performance of poly(lactic acid)(PLA),structurally similar nucleating agents exhibit significant differenc...Although amide-and hydrazide-based nucleating agents have been extensively used to enhance the crystallization performance of poly(lactic acid)(PLA),structurally similar nucleating agents exhibit significant differences in their crystallization-promoting efficiency,and the underlying mechanism remains unclear.In this study,a series of nucleating agents,including N,N-diphenylterephthalamide(DPTA),N,N,N-triphenyl-1,3,5-benzenetricarboxamide(TPTA),N,N-diphenyl terephthalohydrazide(DBTA),and N,N,N-tribenzoyl-1,3,5-benzenetricarbohydrazide(TBTA),were designed and synthesized to investigate the differences in their effects on the crystallization performance of PLA.Density functional theory(DFT)and molecular dynamics(MD)simulations showed that DBTA had a smaller electrostatic potential difference(66.2 kcal/mol).During the cooling process,DBTA could stably form more intermolecular hydrogen bonds with PLA and exhibit a higher interaction energy,thus theoretically enabling more efficient promotion of PLA crystallization.Further differential scanning calorimetry(DSC)results revealed that at a 0.5wt%loading of DBTA,the crystallization peak temperature of the PLA-DBTA composite reached 118.1℃during cooling,whereas no distinct crystallization peak was observed for pure PLA under identical conditions.The crystallinity of the composite was significantly increased to 58.4%compared to 14.6%of pure PLA.Moreover,under isothermal crystallization at 130℃,DBTA reduced the half-crystallization time of PLA to 2.9min,while the half-crystallization time for pure PLA was 27.4 min.Time-resolved Fourier transform infrared spectroscopy(FTIR)results also confirmed that DBTA promoted the formation of gt conformational isomers of PLA during the crystallization process.This study elucidates the mechanism behind the performance differences between structurally similar nucleating agents in regulating PLA crystallization from the perspective of molecular electrostatic potential and hydrogen bonding interactions,providing a theoretical basis for the molecular design of efficient nucleating agents.展开更多
This study aimed to systematically regulate the performance of 4D printing composites by investigating the synergistic effects of dicumyl peroxide(DCP)and maleic anhydride-grafted polyethylene(MAH-g-PE)on a poly(lacti...This study aimed to systematically regulate the performance of 4D printing composites by investigating the synergistic effects of dicumyl peroxide(DCP)and maleic anhydride-grafted polyethylene(MAH-g-PE)on a poly(lactic acid)/thermoplastic polyurethane(PLA/TPU)matrix.Specifically,using a 70 wt%/30 wt%PLA/TPU matrix and an L_(9)(3^(2))orthogonal design,composites were evaluated via morphology,shape memory,mechanical tests,and multi-criteria analysis.Moderate DCP enhanced crosslinking,improving storage modulus and thermal stability,while excessive DCP caused brittleness.Furthermore,MAH-g-PE effectively improved interfacial compatibility,and its synergy with DCP was dosage-dependent.Consequently,Sample 5 achieved optimal performance,exhibiting uniform fracture morphology,a shape fixation rate of98.8%with the fastest recovery,and balanced strength-ductility.Multi-criteria analysis identified elongation at break and recovery time as the top contributing factors,with consistent rankings validated by Spearman analysis(ρ=0.833,p<0.01).In summary,adjusting DCP and MAH-g-PE contents effectively modulates the crosslinking structure and interfacial properties of PLA/TPU composites,providing a viable strategy for developing high-performance,tunable 4D printing materials.展开更多
The application of DNA hybridization technology,grounded in Watson-Crick base pairing,has facilitated the rational design of framework nucleic acids(FNAs)featuring adaptable shapes and dimensions.These nanostructures ...The application of DNA hybridization technology,grounded in Watson-Crick base pairing,has facilitated the rational design of framework nucleic acids(FNAs)featuring adaptable shapes and dimensions.These nanostructures exhibit remarkable stability and reproducibility,making them promising candidates for biomedical applications.Among various FNAs,tetrahedral FNAs(tFNAs),first introduced by Turberfield,are nanoscale assemblies of oligonucleotides that possess unique physical,chemical,and biological properties.Previous studies have demonstrated that tFNAs exhibit excellent cellular uptake,enhanced tissue permeability,and strong capabilities to promote cell migration,proliferation,and differentiation.Moreover,the intrinsic ability of tFNAs to efficiently penetrate cell membranes allows tFNAs to serve as versatile carriers for small-molecule drugs or functional oligonucleotides,thereby exerting significant anti-inflammatory,antioxidant,antibacterial,and immunomodulatory effects.These features highlight the therapeutic potential of tFNA-based complexes in skin,mucosal,and barrier tissue repair and regeneration.This review provides a comprehensive analysis of recent advances in the application of tFNAs for the prevention and treatment of skin,mucosal,and barrier tissue diseases,with a focus on their mechanisms of action and future prospects in regenerative medicine and targeted therapies.展开更多
To address the issue of residual pollution caused by polyethylene mulch,this study explored the effects of different mulching methods on the soil environment of the yam field,as well as on yam yield and quality.The ex...To address the issue of residual pollution caused by polyethylene mulch,this study explored the effects of different mulching methods on the soil environment of the yam field,as well as on yam yield and quality.The experiment comprised six treatments in total:one non-mulched treatment served as the control(CK),along with five different film-mulched treatments,namely PE,FZS12,FZS15,FC12,and FC15.The degradation of these films and their effects on soil physicochemical properties,microbial community,yam yield and quality were compared.The results showed that the FZS12 treatment achieved grade 5 degradation by the end of the planting period.Compared with PE treatment,the total soluble sugar content and yield of yam treated with FZS12 were significantly increased by 35.78%and 74.97%,respectively(p<0.05).Compared with CK and PE treatments,FZS12 significantly increased soil available nitrogen by 31.62%and 6.20%,respectively(p<0.05),and significantly increased soil available phosphorus by 8.58%and 4.45%,respectively(p<0.05).Soil pH,available nitrogen,and available phosphorus were the main environmental factors affecting the soil bacterial community.The FZS12 treatment significantly increased the relative abundances of soil bacteria phylum including Acidobacteriota,Myxococcota,Patescibacteria,and Proteobacteria compared with the CK and PE treatments.Functional prediction using Picrust2 revealed that the FZS12 treatment had significantly higher levels of signal transduction and amino acid metabolism than the CK and PE treatments.In conclusion,covering with 12μm PBAT/PLA humic acid biodegradable film enhances yam yield and total soluble sugar content by shaping beneficial soil microbial communities,activating soil nutrients.展开更多
Peptides play important roles in chemistry,medicinal chemistry and life science,due to their high efficiency and specificity,unusual biological and therapeutic properties.As naturally occurring peptides often face wit...Peptides play important roles in chemistry,medicinal chemistry and life science,due to their high efficiency and specificity,unusual biological and therapeutic properties.As naturally occurring peptides often face with their intrinsic limitations including metabolic instability and low membrane permeability,the strategies for synthesizing unnatural amino acids and peptides are explored.Among the methods for modifying amino acids and peptides,chemo-and site-selective approaches are preferred because of the ability to fine-tuning structural features.Recently,transition metal-catalyzed C–H activation has been employed for the functionalization of amino acids and peptides.Through domino C–H activation/annulation,a series of structurally complex and diverse amino acids and peptides is constructed.This review highlights recent advances in the synthesis of unnatural amino acids and peptides via transition metal-catalyzed C–H activation/annulation.展开更多
Background Heat stress(HS)can impair boar testicular function,leading to reproductive issues.However,chlorogenic acid(CGA)has been shown to mitigate HS-induced damage in various livestock and poultry species.Prepubert...Background Heat stress(HS)can impair boar testicular function,leading to reproductive issues.However,chlorogenic acid(CGA)has been shown to mitigate HS-induced damage in various livestock and poultry species.Prepuberty is an important stage of testicular development in boars after birth.However,the protective effect of CGA on testicular HS injury during prepuberty boars and the underlying mechanisms are still not fully understood.Results In vivo,a total of 30 healthy boars with similar body weights and ages were obtained and randomly divided into 3 groups,which were fed a basal diet supplemented with CGA 0(the ND_TN group),0(the ND_HS group)or 1,000(the CGA_HS group)mg/kg.After being fed for 28 d,all the groups,except the ND_TN group,were treated with high temperature for 7 d,after which samples were collected from the boars and analysed.The results showed that CGA significantly mitigated the HS-induced reduction in T-AOC content in testicular tissue and sperm density.Mechanistically,multiomics analysis revealed that the genes differentially expressed by CGA and HS were predominantly associated with the glutathione metabolism pathway.The combined analysis of transcriptomics and proteomics revealed that only BLVRA was affected by both HS and CGA when the mRNA and protein levels of a gene showed differential expression with the same trend.In vitro studies confirmed that CGA modulated GPX3 expression via BLVRA,affected GPx activity,and attenuated HS-induced ROS accumulation.Conclusions In conclusion,prepubertal HS impairs the spermatogenic capacity of boars.BLVRA may mediate the testicular protective effect of CGA,although in vivo validation of this pathway is needed.This study contributes to elucidating the mechanisms underlying the effects of HS on prepubertal boar testicular development using multiomics approaches,laying a foundation for the potential utilization of CGA in swine production.展开更多
Neurodegenerative disorde rs such as Alzheimer's and Parkinson s diseases are increasingly associated with metabolic dysfunction,including obesity,type 2 diabetes,and metabolic dysfunction-associated steatotic liv...Neurodegenerative disorde rs such as Alzheimer's and Parkinson s diseases are increasingly associated with metabolic dysfunction,including obesity,type 2 diabetes,and metabolic dysfunction-associated steatotic liver disease.Central to this connection is the dysregulation of lipid metabolism,which extends beyond peripheral tissues to the brain,defective autolysosomal function,oxidative stress,inflammation,and insulin resistance.Lipids,which constitute over half of dry weight of the brain,play critical roles in ene rgy provisio n,structural integrity,and synaptic function.Dys regulation of lipid metabolism contributes to neuroinflammation,impaired neuronal function,and disrupted blood-brain barrier integrity.Palmitic acid,a saturated fatty acid abundant in high-fat diets,serves as a key model for studying lipid-induced toxicity(lipotoxicity)in the brain.Palmitic acid disrupts autophagy and lysosomal function,mitochondrial function,trigge ring oxidative stress,contributing to neuroinflammation and neurodegeneration.These effects are particularly pronounced in neurons,which are highly susceptible to lipid-induced toxicity due to their high metabolic demands.Glial cells,including astrocytes,microglia,and oligodendrocytes,also exhibit distinct vulnerabilities and adaptive responses to lipid metabolism dysregulation,further contributing to neuroinflammation and demyelination.Therapeutic strategies,such as supplementation with polyunsaturated fatty acids,AMP-activated protein kinase activation,and lysosome-ta rgeted interventions,show promise in mitigating palmitic acid-induced lipotoxicity and restoring cellular homeostasis.This review comprehensively examines palmitic acid-induced lipotoxicity and its impact on autolysosomal dysfunction across various central nervous system cell types,including neurons,astrocytes,microglia,and oligodendrocytes.Additionally,it highlights therapeutic approaches to restore autolysosomal function under lipotoxic conditions.Advances in multi-omics technologies and a deeper unde rstanding of intercellular crosstalk offer new avenues for develo ping targeted the rapies to resto re autolysosomal function,and attenuate neuroinflammation and neurodegeneration.展开更多
Investigating structural and hydroxyl group effects in electrooxidation of alcohols to value-added products by solid-acid electrocatalysts is essential for upgrading biomass alcohols.Herein,we report efficient electro...Investigating structural and hydroxyl group effects in electrooxidation of alcohols to value-added products by solid-acid electrocatalysts is essential for upgrading biomass alcohols.Herein,we report efficient electrocatalytic oxidations of saturated alcohols(C_(1)-C_(6))to selectively form formate using Ni Co hydroxide(Ni Co-OH)derived Ni Co_(2)O_(4)solid-acid electrocatalysts with balanced Lewis acid(LASs)and Brønsted acid sites(BASs).Thermal treatment transforms BASs-rich(89.6%)Ni Co-OH into Ni Co_(2)O_(4)with nearly equal distribution of LASs(53.1%)and BASs(46.9%)which synergistically promote adsorption and activation of OH-and alcohol molecules for enhanced oxidation activity.In contrast,BASs-enriched Ni Co-OH facilitates formation of higher valence metal sites,beneficial for water oxidation.The combined experimental studies and theoretical calculation imply the oxidation ability of C1-C6alcohols increases as increased number of hydroxyl groups and decreased HOMO-LUMO gaps:methanol(C_(1))<ethylene glycol(C_(2))<glycerol(C3)<meso-erythritol(C4)<xylitol(C5)<sorbitol(C6),while the formate selectivity shows the opposite trend from 100 to 80%.This study unveils synergistic roles of LASs and BASs,as well as hydroxyl group effect in electro-upgrading of alcohols using solid-acid electrocatalysts.展开更多
A critical scientific gap exists in quantifying the intrinsic mechanisms of shale mechanical property degradation induced by the combined effects of perforation(impact)and acidization—two core techniques for shale re...A critical scientific gap exists in quantifying the intrinsic mechanisms of shale mechanical property degradation induced by the combined effects of perforation(impact)and acidization—two core techniques for shale reservoir permeability enhancement.To address this gap,this study proposed an innovative coupled experimental framework integrating dynamic-static cyclic loading(to simulate perforation impact)and acid erosion.Static uniaxial compression tests were performed on treated damaged shale samples,with microstructural characterization via X-ray diffraction(XRD)and scanning electron microscopy(SEM).Key findings include:(1)The damage factor(characterized by longitudinal wave velocity)showed a significant positive correlation with acid concentration;(2)Combined damage(impact+acidization)caused far more severe mechanical deterioration than single damage modes—for instance,samples under combined damage with 20%hydrochloric acid exhibited a strength reduction to 158.97 MPa,with sharp decreases in peak strength and elastic modulus;(3)Damage reduced total energy and elastic strain energy of samples while increasing dissipated energy proportion,leading to more developed internal fractures and severe failure in combined damage samples;(4)Acidization promoted sample fragmentation into smaller debris,resulting in significantly higher fractal dimensions of acidized shale than other damage types under the same acid concentration;(5)XRD and SEM analyses confirmed that high-concentration acid erosion reduced shale carbonate content,and the synergy of mechanical pre-damage and chemical dissolution in combined damage accelerated acid-rock reactions,significantly increasing micro-interfacial pores and degrading shale structural integrity.This study’s innovation lies in establishing a coupled experimental framework that reproduces the actual“perforation-acidization”sequence,quantitatively revealing the synergistic degradation mechanism of shale mechanical properties under combined damage—providing a novel theoretical basis for optimizing shale reservoir stimulation parameters.展开更多
Extracting rare earth elements(REEs)from coal refuse is challenging due to their low co ncentrations and poor leachability.Bioleaching has emerged as a sustainable technology to recover REEs from low-grade materials.I...Extracting rare earth elements(REEs)from coal refuse is challenging due to their low co ncentrations and poor leachability.Bioleaching has emerged as a sustainable technology to recover REEs from low-grade materials.In this study,a ferric sulfate bio acid(BA)with an acidity of~0.2 mol/L H^(+),generated through regulated pyrite bio-oxidation,was used to leach REEs and critical metals(CMs)from coal refuse(ground to<1 mm)after 20-min roasting at 600℃.The influences of solid/liquid(S/L)ratio(100-300 g/L)and leaching temperature(25-95℃)on the leaching performance were examined.The leaching mechanisms were investigated by conducting stepwise precipitation tests and spectroscopic characterization.Results show that raising the leaching temperature to≥65℃accelerated the REE leaching kinetics but causes the loss of light REEs(LREEs)after reaching peak values at 30-60 min.Stage precipitation tests reveal that the loss is due to the incorporation of REEs,especially for LREEs,by gypsum and schwertmannite.The peak total REE(TREE)recovery of the BA leaching reaches 24.9%after 30 min of leaching at75℃with a S/L ratio of 200 g/L.Implementing the three-stage counter-current leaching increases the overall TREE recovery to 31.8%by recovering the REEs incorporated in the Fe and Ca precipitates.Meanwhile,promising recovery values of Li(55.5%),Mn(74.6%),Ni(41.6%),and Co(35.3%)are also achieved.This method provides a sustainable approach to extract REEs and critical metals from coal waste materials with a high treatment capacity.展开更多
基金supported by the National Key R&D Program of China,No.2021YFC2501200(to PC).
文摘Short-chain fatty acids,metabolites produced by the fermentation of dietary fiber by gut microbiota,have garnered significant attention due to their correlation with neurodegenerative diseases,particularly Parkinson’s disease.In this review,we summarize the changes in short-chain fatty acid levels and the abundance of short-chain fatty acid-producing bacteria in various samples from patients with Parkinson’s disease,highlighting the critical role of gut homeostasis imbalance in the pathogenesis and progression of the disease.Focusing on the nervous system,we discuss the molecular mechanisms by which short-chain fatty acids influence the homeostasis of both the enteric nervous system and the central nervous system.We identify key processes,including the activation of G protein-coupled receptors and the inhibition of histone deacetylases by short-chain fatty acids.Importantly,structural or functional disruptions in the enteric nervous system mediated by these fatty acids may lead to abnormalα-synuclein expression and gastrointestinal dysmotility,which could serve as an initiating event in Parkinson’s disease.Furthermore,we propose that short-chain fatty acids help establish communication between the enteric nervous system and the central nervous system via the vagal nerve,immune circulation,and endocrine signaling.This communication may shed light on their potential role in the transmission ofα-synuclein from the gut to the brain.Finally,we elucidate novel treatment strategies for Parkinson’s disease that target short-chain fatty acids and examine the challenges associated with translating short-chain fatty acid-based therapies into clinical practice.In conclusion,this review emphasizes the pivotal role of short-chain fatty acids in regulating gut-brain axis integrity and their significance in the pathogenesis of Parkinson’s disease from the perspective of the nervous system.Moreover,it highlights the potential value of short-chain fatty acids in early intervention for Parkinson’s disease.Future research into the molecular mechanisms of short-chain fatty acids and their synergistic interactions with other gut metabolites is likely to advance the clinical translation of innovative short-chain fatty acid-based therapies for Parkinson’s disease.
文摘Three copper(Ⅱ),nickel and cadmium(Ⅱ)complexes,namely[Cu_(2)(μ-H2dbda)2(phen)2]·2H_(2)O(1),[Ni(μ-H2dbda)(μ-bpb)(H_(2)O)2]n(2),and[Cd(μ-H2dbda)(μ-bpa)]n(3),have been constructed hydrothermally using H4dbda(4,4'-dihydroxy-[1,1'-biphenyl]-3,3'-dicarboxylic acid),phen(1,10-phenanthroline),bpb(1,4-bis(pyrid-4-yl)benzene),bpa(bis(4-pyridyl)amine),and copper,nickel and cadmium chlorides at 160℃.The products were isolated as stable crystalline solids and were characterized by IR spectra,elemental analyses,thermogravimetric analyses,and singlecrystal X-ray diffraction analyses.Single-crystal X-ray diffraction analyses revealed that three complexes crystallize in the monoclinic P21/n,tetragonal I42d,and orthorhombic P21212 space groups.The complexes exhibit molecular dimers(1)or 2D metal-organic networks(2 and 3).The catalytic performances in the Knoevenagel reaction of these complexes were investigated.Complex 1 exhibits an effective catalytic activity and excellent reusability as a heterogeneous catalyst in the Knoevenagel reaction at room temperature.CCDC:2463800,1;2463801,2;2463802,3.
基金Supported by the Science and Technology Major Project of Liaoning Province(2024JH2,1025000892)the Fundamental Research Funds for the Universities of Liaoning Province(LJ232410143051)+1 种基金Liaoning Provincial Science and Technology Program 2023JHl/10400006Shenyang Science and Technology Program(24-213-3-09)。
文摘Methane dehydroaromatization(MDA)presents a promising carbon-neutral pathway for benzene,toluene,and xylene(BTX)production,alternative to petroleumderived routes.Elucidating the regulatory mechanisms of Brønsted acid site(BAS)strength on reaction pathways,alongside the spatial proximity effects between BAS and Mo active sites in bifunctional synergy,remains a critical scientific challenge in catalyst design.This study systematically tunes both BAS strength(via isomorphous metal substitution)and Mo-BAS spatial proximity in zeolites,integrating MDA catalytic evaluations with density functional theory(DFT)calculations to dissect their individual contributions.Strongly acidic BAS catalysts(compared to moderately acidic Fe/Ga-substituted counterparts)exhibit superior performance,evidenced by enhanced aromatic yields.Conversely,weakly acidic Bsubstituted zeolites demonstrate optimal mono-/bifunctional synergy,outperforming moderate-acid systems.DFT results reveal that acid strength dictates C−H activation mechanisms by modulating the energy barriers of rate-determining steps.While Al-zeolites deliver the highest activity,B-substituted systems display unique potential for mechanistic investigations.Spatial proximity analysis indicates that micrometer-scale Mo-BAS distances hinder effective synergy due to exceeding electron interaction and mass transfer limits,whereas nanometer-scale proximity enhances activity(via accelerated intermediate transport)and suppresses coke formation.These findings establish a theoretical framework for rationalizing zeolite catalyst optimization through BAS property engineering and spatial control of Mo-BAS cooperation,providing actionable guidelines for designing next-generation MDA catalysts.
文摘This study employed integrated multi-omics approaches to elucidate,from the perspective of amino acid metabolism,the adaptive mechanism of Penicillium digitatum under modified atmosphere packaging(MAP)conditions.Comparative analysis of natural air(Air),controlled atmosphere(CA),and MAP treatments revealed that MAP upregulated the expression of the hercynylcysteine S-oxide synthase(HCSOS),aldehyde dehydrogenase(ALDH),and monoamine oxidase(MAO)genes,thereby enhancing histidine-derived ergothioneine and methionine levels,and subsequently boosting glutathione-mediated redox homeostasis.Meanwhile,MAP induced the expression of the dihydroxyacid dehydratase(DHAD),saccharopine dehydrogenase(SDH),and arginosuccinate lyase(ASL)genes,redirecting valine,lysine,and arginine into the tricarboxylic acid(TCA)cycle to fuel ATP production.MAP also enhanced ASL-mediated arginine degradation and urea cycle activity,reducing arginine accumulation when compared to CA treatment.In contrast,while MAP induced upregulated expression of the pyrroline-5-carboxylate dehydrogenase(P5CDH)and D-amino acid oxidase(DAAO)genes,CA treatment promoted proline accumulation,reflecting stress-specific metabolic flexibility.Collectively,these findings demonstrate that MAP triggers transcriptional reprogramming of amino acid metabolism to coordinate oxidative defense,energy generation,and osmotic balance.By modulating these metabolic pathways and regulatory genes under MAP conditions,fungal adaptability can be disrupted.Hence,this study provides a promising strategy for suppressing green mold development,extending the postharvest shelf life,and improving the quality of fruits and vegetables.
基金Supported by National Natural Science Foundation(52374279)。
文摘The efficient extraction and separation of valuable metal elements from coal gasification fine slag(CGFS)are crucial for the comprehensive high-value utilization of its constituents.This study focused on the carbon-rich components of CGFS(CGFS-H)and systematically investigates the selective leaching behavior of Fe^(3+),Al^(3+)and Ca^(2+)using three organic acid extractants,i.e.,citric acid,tartaric acid,and tetrasodium iminodisuccinate.Additionally,the stepwise leaching of iron,aluminum and calcium from CGFS-H is explored.The selective dissolution mechanisms of these metals by different organic acids are elucidated through X-ray diffraction(XRD),X-ray fluorescence(XRF),and scanning electron microscopy(SEM)analyses.The results indicate that tetrasodium iminodisuccinate exhibits the highest leaching selectivity for Fe^(3+),while tartaric acid demonstrateds a comparable affinity for both Fe^(3+)and Al^(3+).In contrast citric acid shows superior selectivity toward Ca^(2+).The leaching yield of Fe^(3+),Al^(3+)and Ca^(2+)after sequential leaching with the three organic acids were 79.8%,65.08%and 78.6%,respectively.These findings confirm that effective and selective separation of Fe^(3+),Al^(3+)and Ca^(2+)from CGFS-H can be achieved via optimized organic acid-based leaching strategies.This advancement provides a critical foundation for developing Ca/Fe/Al hydrotalcite materials using CGFS-H as a sustainable feedstock,thereby facilitating the transformation of waste residue into high-value functional materials and promoting resourceefficient utilization of coal gasification fine slag.
文摘The hydrated tricyclohexyltin theophylline-7-acetic acid(tpH)complex[Sn(C_(6)H_(11))_(3)(tp)(H_(2)O)]was synthesized via an ethanol solvothermal method using tricyclohexyltin hydroxide and tpH in a 1∶1 molar ratio.The complex was characterized by IR,^(1)H(^(13)C)NMR,elemental analysis,and powder X-ray diffraction,and the crystal structure was determined by single-crystal X-ray diffraction.The crystal belongs to the orthorhombic system with space group Iba2,and the central tin atom is in a five-coordinated trigonal bipyramidal configuration.Quantum chemistry ab initio calculations were performed to investigate the stability,molecular orbital energy,and frontier molecular orbital characteristics of the complex.Additionally,its thermal stability,electrochemical properties,and in vitro anticancer activity were evaluated.CCDC:2380308.
基金supported by the National Natural Science Foundation of China(82204790)Shaanxi University of Chinese Medicine Science and Technology Program(2024-LJRC-04)+1 种基金Shaanxi Province Natural Science Foundation(2024JC-YBMS-759)Qinchuangyuan TCM Industry Innovation Project(L2024-QCY-ZYYJJQ-X29).
文摘Background:This study aimed to comprehensively elucidate the hepatotoxic effects of Dioscorea bulbifera L.(DBL)in mice and to explore the underlying molecular mechanisms.Methods:Mice were administered different doses of DBL via intragastric gavage for 21 days.Organ indices of multiple organs were then measured,and histopathological changes were systematically evaluated.Particular attention was paid to biochemical markers and hepatic histopathological alterations associated with liver injury.Subsequently,the composition of the gut microbiota in colonic contents was analyzed using 16S rDNA sequencing,and the levels of short-chain fatty acids(SCFAs)were determined by gas chromatography(GC).Furthermore,protein expression levels of YinYang 1(YY1),Farnesoid X Receptor(FXR),Nuclear factor erythroid 2-related factor 2(Nrf2),and bile acids(BAs)-related proteins in the liver were assessed by Western blot analysis.Results:Our findings revealed that DBL induced varying degrees of multi-organ toxicity in mice,with the most pronounced hepatotoxicity observed.Liver injury was induced in a dose-dependent manner,accompanied by increased hepatic inflammation and oxidative stress,as well as enhanced hepatocyte apoptosis.Furthermore,DBL may modulate the YY1-FXR/Nrf2 signaling pathway,leading to upregulation of BAs-related proteins such as cholesterol 7α-hydroxylase(CYP7A1),while downregulating other BAs-related proteins,including sodium taurocholate cotransporting polypeptide(NTCP),bile salt export pump(BSEP),multidrug resistance protein 2(MRP2),and P-glycoprotein(P-gp).These changes promoted excessive BAs synthesis and impaired the excretion and reabsorption of BAs,ultimately disturbing BAs homeostasis.Additionally,DBL induces gut microbiota dysbiosis and reduces SCFAs production,leading to intestinal damage and disruption of intestinal barrier integrity.Conclusion:DBL-induced hepatotoxicity may be associated with altered expression of the YY1-FXR/Nrf2 signaling pathway and BAs–related proteins,accompanied by increased hepatic BAs accumulation and gut microbiota dysbiosis.
基金supported by National Key R&D Program of China(2021YFD2100700).
文摘Both linoleic acid(18:2 n-6,LA)andα-linolenic acid(18:3 n-3,ALA)are essential fatty acids for infants.The contents of LA and ALA,and their ratio exhibited significant changes in human milk over the past 4 decades,which were not well summarized.Here,we summarized these values in 9898 human breast milk samples of 6664 mothers from 50 countries in 81 studies.A literature search was conducted using PubMed,Embase,and Web of Science between January 1980 and October 2023.The 95%confidence interval of LA/ALA ratio across lactation and gestation ranged from 14.24 to 31.26.The LA content was higher in China and Turkey(>20%)whereas the ALA content was below 1%in Africa.The LA/ALA ratio in countries along the Mediterranean coast exceeded 20 or even 30.LA and ALA contents increased significantly(P<0.01)while the ratio remained stable over the last 40 years.Multivariate meta-regression results showed that regions significantly(P<0.01)determined the LA,ALA,and LA/ALA ratio.Especially,maternal diet could definitely explain the variation while the effects of gestational age,lactation period was not significant.Clinical trials demonstrated that decreasing the LA/ALA ratio increased docosahexaenoic acid(22:6 n-3,DHA)status,reduced arachidonic acid(20:4 n-6,AA)contents,exerted no effect on the visual function of infants,and reached no consensus on growth.The current review aims to provide an overview on the LA and ALA contents and their ratio in human breast milk to raise concern in infant formula.
基金supported by the National Science Fund for Excellent Young Scholars(grant number 32422082)the Natural Science Basic Research Plan in Shaanxi Province(grant number 2025JC-QYXQ-009)。
文摘Background Ruminants and monogastric animals exhibit significant differences in gluconeogenic efficiency.In dairy cows,hepatic gluconeogenesis serves as the primary source of glucose.Metabolites modulate gluconeogenesis efficiency through allosteric regulation,redox state,and signal transduction pathways.However,the liver-enriched metabolites that regulate hepatic gluconeogenesis in dairy cows and their specific regulatory mechanisms remain incompletely characterized.Results Six Holstein dairy cows and six Duroc×(Landrace×Yorkshire)(DLY)crossbred pigs served as research subjects.Employing non-targeted and targeted metabolomics,we discovered that three bile acids—taurodeoxycholic acid(TDCA),taurocholic acid(TCA),and glycocholic acid(GCA)—were highly enriched in Holstein dairy cows'livers.In bovine hepatocytes,individual or combined stimulation of these bile acids significantly upregulated the expression of gluconeogenesis genes(FBP1,PCK1 and G6PC)and enhanced glucose production.In fasting mice with induced gluconeogenesis,TDCA,TCA,and GCA increased fasting blood glucose levels,and pyruvate tolerance tests further revealed their capacity to enhance hepatic gluconeogenesis,enabling more efficient glucose synthesis from pyruvate.Mechanistically,these bile acids activated Takeda G protein-coupled receptor 5(TGR5),elevated intracellular cAMP levels,and ultimately enhanced gluconeogenesis via the transcription factor cAMP-response element binding protein(CREB).Notably,a TGR5 inhibitor abrogated the stimulatory effects of TDCA,TCA,and GCA on hepatic gluconeogenesis in fasting mice.Conclusion TDCA,TCA,and GCA are key metabolites promoting hepatic gluconeogenesis in dairy cows,with TGR5 as the pivotal receptor and the cAMP/PKA/CREB pathway as the critical downstream mechanism.
基金supported by National Key R&D Program of China(2021YFD2100300)Pilot Research Project of Wuxi Industrial Innovation Research Institute(XD24019).
文摘In previous research,we demonstrated that long-term consumption of thermally oxidized oil leads to neuroinflammation and anxiety in mice.Therefore,in this study,we employed polar lipid components from thermo-induced oxidized oil to induce neurodamage.Behavioral assessments revealed that both the linoleic acid and AUDA(a classical inhibitor of soluble epoxide hydrolase)groups exhibited significantly reduced anxiety-like behaviors compared to the model group(P<0.05).Immunofluorescence analysis indicated that microglial activation in the hippocampus was attenuated in both the linoleic acid and AUDA groups relative to the model group,accompanied by a reduction in the m RNA expression of pro-inflammatory cytokines(IL-1β,IL-6,NOS2,TNF-α)and an upregulation of neuroprotective factors(IL-4,IL-10,BDNF).Lipidomic profiling of hippocampal tissue revealed that the lipid composition of the linoleic acid group closely resembled that of the AUDA group,with a significant downregulation of cardiolipin(CL)compared to the control group,consistent with alterations in the membrane potential channel receptor TRPC1.Both linoleic acid and AUDA inhibited the m RNA expression of EPHX2,leading to an increase in epoxyeicosatrienoic acids(EETs)levels.Furthermore,linoleic acid upregulated the expression of cytochrome P450 enzymes(CYP2J6)and lipoxygenase(LOX2S),which further upregulated the synthesis of EETs,and increased the content of 9-HODE and 13-HODE.These findings collectively suggest that linoleic acid alleviates neuroinflammation by modulating microglial differentiation and attenuates neurodegeneration induced by thermally oxidized oil through the regulation of arachidonic acid metabolism and the linoleic acid metabolic pathway,leading to the production of neuroprotective lipid mediators.Therefore,linoleic acid may serve as a potential neuro-nutrient for the treatment of anxiety disorders.This provided a scientific basis for the development of specialized medical foods aimed at protecting neural health.
基金supported by the Project of the National Science Foundation of China(Nos.52173033,51773044 and 51603047)Foshan Science and Technology Innovation Project(No.FS0AA-KJ919-4402-0145)。
文摘Although amide-and hydrazide-based nucleating agents have been extensively used to enhance the crystallization performance of poly(lactic acid)(PLA),structurally similar nucleating agents exhibit significant differences in their crystallization-promoting efficiency,and the underlying mechanism remains unclear.In this study,a series of nucleating agents,including N,N-diphenylterephthalamide(DPTA),N,N,N-triphenyl-1,3,5-benzenetricarboxamide(TPTA),N,N-diphenyl terephthalohydrazide(DBTA),and N,N,N-tribenzoyl-1,3,5-benzenetricarbohydrazide(TBTA),were designed and synthesized to investigate the differences in their effects on the crystallization performance of PLA.Density functional theory(DFT)and molecular dynamics(MD)simulations showed that DBTA had a smaller electrostatic potential difference(66.2 kcal/mol).During the cooling process,DBTA could stably form more intermolecular hydrogen bonds with PLA and exhibit a higher interaction energy,thus theoretically enabling more efficient promotion of PLA crystallization.Further differential scanning calorimetry(DSC)results revealed that at a 0.5wt%loading of DBTA,the crystallization peak temperature of the PLA-DBTA composite reached 118.1℃during cooling,whereas no distinct crystallization peak was observed for pure PLA under identical conditions.The crystallinity of the composite was significantly increased to 58.4%compared to 14.6%of pure PLA.Moreover,under isothermal crystallization at 130℃,DBTA reduced the half-crystallization time of PLA to 2.9min,while the half-crystallization time for pure PLA was 27.4 min.Time-resolved Fourier transform infrared spectroscopy(FTIR)results also confirmed that DBTA promoted the formation of gt conformational isomers of PLA during the crystallization process.This study elucidates the mechanism behind the performance differences between structurally similar nucleating agents in regulating PLA crystallization from the perspective of molecular electrostatic potential and hydrogen bonding interactions,providing a theoretical basis for the molecular design of efficient nucleating agents.
基金supported by the National Natural Science Foundation of China(No.51905543)。
文摘This study aimed to systematically regulate the performance of 4D printing composites by investigating the synergistic effects of dicumyl peroxide(DCP)and maleic anhydride-grafted polyethylene(MAH-g-PE)on a poly(lactic acid)/thermoplastic polyurethane(PLA/TPU)matrix.Specifically,using a 70 wt%/30 wt%PLA/TPU matrix and an L_(9)(3^(2))orthogonal design,composites were evaluated via morphology,shape memory,mechanical tests,and multi-criteria analysis.Moderate DCP enhanced crosslinking,improving storage modulus and thermal stability,while excessive DCP caused brittleness.Furthermore,MAH-g-PE effectively improved interfacial compatibility,and its synergy with DCP was dosage-dependent.Consequently,Sample 5 achieved optimal performance,exhibiting uniform fracture morphology,a shape fixation rate of98.8%with the fastest recovery,and balanced strength-ductility.Multi-criteria analysis identified elongation at break and recovery time as the top contributing factors,with consistent rankings validated by Spearman analysis(ρ=0.833,p<0.01).In summary,adjusting DCP and MAH-g-PE contents effectively modulates the crosslinking structure and interfacial properties of PLA/TPU composites,providing a viable strategy for developing high-performance,tunable 4D printing materials.
基金supported by the National Natural Science Foundation of China(No.81960199)Clinical Translational Innovation Cultivating Fund 550 Project of Hainan General Hospital,Joint Program on Health Science&Technology Innovation of Hainan Province(No.WSJK2024MS127)Academic Enhancement Support Program of Hainan Medical University(No.XSTS2025093).
文摘The application of DNA hybridization technology,grounded in Watson-Crick base pairing,has facilitated the rational design of framework nucleic acids(FNAs)featuring adaptable shapes and dimensions.These nanostructures exhibit remarkable stability and reproducibility,making them promising candidates for biomedical applications.Among various FNAs,tetrahedral FNAs(tFNAs),first introduced by Turberfield,are nanoscale assemblies of oligonucleotides that possess unique physical,chemical,and biological properties.Previous studies have demonstrated that tFNAs exhibit excellent cellular uptake,enhanced tissue permeability,and strong capabilities to promote cell migration,proliferation,and differentiation.Moreover,the intrinsic ability of tFNAs to efficiently penetrate cell membranes allows tFNAs to serve as versatile carriers for small-molecule drugs or functional oligonucleotides,thereby exerting significant anti-inflammatory,antioxidant,antibacterial,and immunomodulatory effects.These features highlight the therapeutic potential of tFNA-based complexes in skin,mucosal,and barrier tissue repair and regeneration.This review provides a comprehensive analysis of recent advances in the application of tFNAs for the prevention and treatment of skin,mucosal,and barrier tissue diseases,with a focus on their mechanisms of action and future prospects in regenerative medicine and targeted therapies.
基金supported by the Wencheng County Science and Technology Plan Project(2023NKY03)Earmarked Fund for Modern Agro-industry Technology Research System(Grant Number CARS-24-B04,CARS-23-B05)Additional support was provided by Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Vegetables),Ministry of Agriculture and Rural Affairs,China.
文摘To address the issue of residual pollution caused by polyethylene mulch,this study explored the effects of different mulching methods on the soil environment of the yam field,as well as on yam yield and quality.The experiment comprised six treatments in total:one non-mulched treatment served as the control(CK),along with five different film-mulched treatments,namely PE,FZS12,FZS15,FC12,and FC15.The degradation of these films and their effects on soil physicochemical properties,microbial community,yam yield and quality were compared.The results showed that the FZS12 treatment achieved grade 5 degradation by the end of the planting period.Compared with PE treatment,the total soluble sugar content and yield of yam treated with FZS12 were significantly increased by 35.78%and 74.97%,respectively(p<0.05).Compared with CK and PE treatments,FZS12 significantly increased soil available nitrogen by 31.62%and 6.20%,respectively(p<0.05),and significantly increased soil available phosphorus by 8.58%and 4.45%,respectively(p<0.05).Soil pH,available nitrogen,and available phosphorus were the main environmental factors affecting the soil bacterial community.The FZS12 treatment significantly increased the relative abundances of soil bacteria phylum including Acidobacteriota,Myxococcota,Patescibacteria,and Proteobacteria compared with the CK and PE treatments.Functional prediction using Picrust2 revealed that the FZS12 treatment had significantly higher levels of signal transduction and amino acid metabolism than the CK and PE treatments.In conclusion,covering with 12μm PBAT/PLA humic acid biodegradable film enhances yam yield and total soluble sugar content by shaping beneficial soil microbial communities,activating soil nutrients.
基金supported by the Natural Science Foundation of Jiangsu Province(No.BK20220409)the National Natural Science Foundation of China(No.22401153)+2 种基金the FWO[Fund for Scientific Research-Flanders(Belgium)]for financial support(recipient Erik V.Van der Eycken)the Research Council of the KU Leuven(recipient Erik V.Van der Eycken)the support of the"RUDN University Strategic Academic Leadership Program"(recipient Erik V.Van der Eycken).
文摘Peptides play important roles in chemistry,medicinal chemistry and life science,due to their high efficiency and specificity,unusual biological and therapeutic properties.As naturally occurring peptides often face with their intrinsic limitations including metabolic instability and low membrane permeability,the strategies for synthesizing unnatural amino acids and peptides are explored.Among the methods for modifying amino acids and peptides,chemo-and site-selective approaches are preferred because of the ability to fine-tuning structural features.Recently,transition metal-catalyzed C–H activation has been employed for the functionalization of amino acids and peptides.Through domino C–H activation/annulation,a series of structurally complex and diverse amino acids and peptides is constructed.This review highlights recent advances in the synthesis of unnatural amino acids and peptides via transition metal-catalyzed C–H activation/annulation.
基金supported by The Program of Science and Technology Development Plan of Jilin Province(20230202066NC)Graduate Innovation Research Project of Jilin University(2024CX151)。
文摘Background Heat stress(HS)can impair boar testicular function,leading to reproductive issues.However,chlorogenic acid(CGA)has been shown to mitigate HS-induced damage in various livestock and poultry species.Prepuberty is an important stage of testicular development in boars after birth.However,the protective effect of CGA on testicular HS injury during prepuberty boars and the underlying mechanisms are still not fully understood.Results In vivo,a total of 30 healthy boars with similar body weights and ages were obtained and randomly divided into 3 groups,which were fed a basal diet supplemented with CGA 0(the ND_TN group),0(the ND_HS group)or 1,000(the CGA_HS group)mg/kg.After being fed for 28 d,all the groups,except the ND_TN group,were treated with high temperature for 7 d,after which samples were collected from the boars and analysed.The results showed that CGA significantly mitigated the HS-induced reduction in T-AOC content in testicular tissue and sperm density.Mechanistically,multiomics analysis revealed that the genes differentially expressed by CGA and HS were predominantly associated with the glutathione metabolism pathway.The combined analysis of transcriptomics and proteomics revealed that only BLVRA was affected by both HS and CGA when the mRNA and protein levels of a gene showed differential expression with the same trend.In vitro studies confirmed that CGA modulated GPX3 expression via BLVRA,affected GPx activity,and attenuated HS-induced ROS accumulation.Conclusions In conclusion,prepubertal HS impairs the spermatogenic capacity of boars.BLVRA may mediate the testicular protective effect of CGA,although in vivo validation of this pathway is needed.This study contributes to elucidating the mechanisms underlying the effects of HS on prepubertal boar testicular development using multiomics approaches,laying a foundation for the potential utilization of CGA in swine production.
基金the Department of Biology at Syracuse University(to CHL)a start-up grant from the Department of Biomedical and Chemical Engineering at Syacuse University(to JZ)an NIH grant(R01DK141923)sub-contract to Co-Investigators JZ and CHL from the Principal Investigator Mark W.Grinstaff。
文摘Neurodegenerative disorde rs such as Alzheimer's and Parkinson s diseases are increasingly associated with metabolic dysfunction,including obesity,type 2 diabetes,and metabolic dysfunction-associated steatotic liver disease.Central to this connection is the dysregulation of lipid metabolism,which extends beyond peripheral tissues to the brain,defective autolysosomal function,oxidative stress,inflammation,and insulin resistance.Lipids,which constitute over half of dry weight of the brain,play critical roles in ene rgy provisio n,structural integrity,and synaptic function.Dys regulation of lipid metabolism contributes to neuroinflammation,impaired neuronal function,and disrupted blood-brain barrier integrity.Palmitic acid,a saturated fatty acid abundant in high-fat diets,serves as a key model for studying lipid-induced toxicity(lipotoxicity)in the brain.Palmitic acid disrupts autophagy and lysosomal function,mitochondrial function,trigge ring oxidative stress,contributing to neuroinflammation and neurodegeneration.These effects are particularly pronounced in neurons,which are highly susceptible to lipid-induced toxicity due to their high metabolic demands.Glial cells,including astrocytes,microglia,and oligodendrocytes,also exhibit distinct vulnerabilities and adaptive responses to lipid metabolism dysregulation,further contributing to neuroinflammation and demyelination.Therapeutic strategies,such as supplementation with polyunsaturated fatty acids,AMP-activated protein kinase activation,and lysosome-ta rgeted interventions,show promise in mitigating palmitic acid-induced lipotoxicity and restoring cellular homeostasis.This review comprehensively examines palmitic acid-induced lipotoxicity and its impact on autolysosomal dysfunction across various central nervous system cell types,including neurons,astrocytes,microglia,and oligodendrocytes.Additionally,it highlights therapeutic approaches to restore autolysosomal function under lipotoxic conditions.Advances in multi-omics technologies and a deeper unde rstanding of intercellular crosstalk offer new avenues for develo ping targeted the rapies to resto re autolysosomal function,and attenuate neuroinflammation and neurodegeneration.
基金the financial support from the National Natural Science Foundation of China(52172110,52472231,52311530113)Shanghai"Science and Technology Innovation Action Plan"intergovernmental international science and technology cooperation project(23520710600)+1 种基金Science and Technology Commission of Shanghai Municipality(22DZ1205600)the Central Guidance on Science and Technology Development Fund of Zhejiang Province(2024ZY01011)。
文摘Investigating structural and hydroxyl group effects in electrooxidation of alcohols to value-added products by solid-acid electrocatalysts is essential for upgrading biomass alcohols.Herein,we report efficient electrocatalytic oxidations of saturated alcohols(C_(1)-C_(6))to selectively form formate using Ni Co hydroxide(Ni Co-OH)derived Ni Co_(2)O_(4)solid-acid electrocatalysts with balanced Lewis acid(LASs)and Brønsted acid sites(BASs).Thermal treatment transforms BASs-rich(89.6%)Ni Co-OH into Ni Co_(2)O_(4)with nearly equal distribution of LASs(53.1%)and BASs(46.9%)which synergistically promote adsorption and activation of OH-and alcohol molecules for enhanced oxidation activity.In contrast,BASs-enriched Ni Co-OH facilitates formation of higher valence metal sites,beneficial for water oxidation.The combined experimental studies and theoretical calculation imply the oxidation ability of C1-C6alcohols increases as increased number of hydroxyl groups and decreased HOMO-LUMO gaps:methanol(C_(1))<ethylene glycol(C_(2))<glycerol(C3)<meso-erythritol(C4)<xylitol(C5)<sorbitol(C6),while the formate selectivity shows the opposite trend from 100 to 80%.This study unveils synergistic roles of LASs and BASs,as well as hydroxyl group effect in electro-upgrading of alcohols using solid-acid electrocatalysts.
基金funded by the National Natural Science Foundation of China(No.52474120)the National Key Research and Development Program of China—2023 Key Special Project(No.2023YFC2907400)+1 种基金the Fundamental Research Funds for the Central Universities(No.2023CXQD045)the Hunan Provincial Natural Science Foundation for Distinguished Young Scholars(No.2023JJ10072).
文摘A critical scientific gap exists in quantifying the intrinsic mechanisms of shale mechanical property degradation induced by the combined effects of perforation(impact)and acidization—two core techniques for shale reservoir permeability enhancement.To address this gap,this study proposed an innovative coupled experimental framework integrating dynamic-static cyclic loading(to simulate perforation impact)and acid erosion.Static uniaxial compression tests were performed on treated damaged shale samples,with microstructural characterization via X-ray diffraction(XRD)and scanning electron microscopy(SEM).Key findings include:(1)The damage factor(characterized by longitudinal wave velocity)showed a significant positive correlation with acid concentration;(2)Combined damage(impact+acidization)caused far more severe mechanical deterioration than single damage modes—for instance,samples under combined damage with 20%hydrochloric acid exhibited a strength reduction to 158.97 MPa,with sharp decreases in peak strength and elastic modulus;(3)Damage reduced total energy and elastic strain energy of samples while increasing dissipated energy proportion,leading to more developed internal fractures and severe failure in combined damage samples;(4)Acidization promoted sample fragmentation into smaller debris,resulting in significantly higher fractal dimensions of acidized shale than other damage types under the same acid concentration;(5)XRD and SEM analyses confirmed that high-concentration acid erosion reduced shale carbonate content,and the synergy of mechanical pre-damage and chemical dissolution in combined damage accelerated acid-rock reactions,significantly increasing micro-interfacial pores and degrading shale structural integrity.This study’s innovation lies in establishing a coupled experimental framework that reproduces the actual“perforation-acidization”sequence,quantitatively revealing the synergistic degradation mechanism of shale mechanical properties under combined damage—providing a novel theoretical basis for optimizing shale reservoir stimulation parameters.
文摘Extracting rare earth elements(REEs)from coal refuse is challenging due to their low co ncentrations and poor leachability.Bioleaching has emerged as a sustainable technology to recover REEs from low-grade materials.In this study,a ferric sulfate bio acid(BA)with an acidity of~0.2 mol/L H^(+),generated through regulated pyrite bio-oxidation,was used to leach REEs and critical metals(CMs)from coal refuse(ground to<1 mm)after 20-min roasting at 600℃.The influences of solid/liquid(S/L)ratio(100-300 g/L)and leaching temperature(25-95℃)on the leaching performance were examined.The leaching mechanisms were investigated by conducting stepwise precipitation tests and spectroscopic characterization.Results show that raising the leaching temperature to≥65℃accelerated the REE leaching kinetics but causes the loss of light REEs(LREEs)after reaching peak values at 30-60 min.Stage precipitation tests reveal that the loss is due to the incorporation of REEs,especially for LREEs,by gypsum and schwertmannite.The peak total REE(TREE)recovery of the BA leaching reaches 24.9%after 30 min of leaching at75℃with a S/L ratio of 200 g/L.Implementing the three-stage counter-current leaching increases the overall TREE recovery to 31.8%by recovering the REEs incorporated in the Fe and Ca precipitates.Meanwhile,promising recovery values of Li(55.5%),Mn(74.6%),Ni(41.6%),and Co(35.3%)are also achieved.This method provides a sustainable approach to extract REEs and critical metals from coal waste materials with a high treatment capacity.
