Background: Anesthetic agents are commonly utilized in the handling of non‐human primates for prevent the stress caused in physical exploration or physical restrain. For this reason, the objective of this work was to...Background: Anesthetic agents are commonly utilized in the handling of non‐human primates for prevent the stress caused in physical exploration or physical restrain. For this reason, the objective of this work was to describe the effect of age and dissociative anesthetics (ketamine and tiletamine), and their combinations with acepromazine, xylazine and zolazepam, on the physiological and blood biochemical parameters in Macaca mulatta. Methods: Eighty male Macaca mulatta were divided into four experimental groups depending on the anesthetic mixture applied. Each group of 20 males was divided into five sub‐groups according to age. Physiological parameters were recorded every five minutes during a 30‐minute period. A blood sample was drawn to analyze blood biochemistry. Results: Statistical analyses revealed significant differences in the physiological parameters between the ketamine‐acepromazine and ketamine‐xylazine groups compared to the control group. The analysis of blood biochemistry found significant differences by age and by anesthetic mixture among all groups. Conclusion: These findings contribute to standardizing this animal model in biological research.展开更多
Targeted protein degradation (TPD) revolutionizes drug discovery by taking advantages of event-driven mechanism of action (MOA). Among current proximity-inducing platforms for TPD, molecular glue degraders and proteol...Targeted protein degradation (TPD) revolutionizes drug discovery by taking advantages of event-driven mechanism of action (MOA). Among current proximity-inducing platforms for TPD, molecular glue degraders and proteolysis-targeting chimeras (PROTACs) are representative strategies that facilitate interactions between an E3 ligase and a specific protein target. This leads to ubiquitination and subsequent degradation of the target by the ubiquitin–proteasome system (UPS). Despite offering unprecedented opportunities for more effective and targeted therapies, current UPS-hijacking TPD still faces key challenges. For instance, although over 600 E3 ligases have been identified in human cells, only a handful, such as cereblon (CRBN) and von Hippel-Lindau (VHL), are accessible for developing degraders1. In addition, there are ongoing questions about how to further expand the chemically tractable target space beyond monomeric proteins with cytosolic domains.展开更多
The present study was conduct to compare different oral administration of medication to induce sedation in dogs. In the domestic dogs,the drugs of tiletamine-zolazepam and acepromazine (TZA) through oral administratio...The present study was conduct to compare different oral administration of medication to induce sedation in dogs. In the domestic dogs,the drugs of tiletamine-zolazepam and acepromazine (TZA) through oral administration were used to induce sedation in dogs. Three administration methods were in order directly feeding liquid drugs,encapsulated drugs and liquid drugs mixed with cooked gravy. In the domestic dogs (n = 6),the results showed that the time to sedation score of 4 (sternal recumbency,reluctant to stand) and the capture ratio of dogs in three administration methods were 23 ± 25 min and 83%,27 ± 20 min and 83%,56 ± 27 min and 100%. The time to sedation score of 5 (lateral recumbency,unable to maintain sternal recumbency) and the capture ratio of dogs in three administration methods were 53 ± 22 min and 67%,32 ± 20 min and 83%,730 ± 25 min and 100%,respectively. In the field dogs,17 of 27 dogs feed the TZA drug mixed with cooked gravy or packaged into aspic achieved sedation score of 4 and further successfully captured. In conclusion,the drugs of tiletamine-zolazepam and acepromazine (TZA) through oral administration could be safely and successfully captured the dog in field,and furthermore the palatability of drugs would apparently affect the capturing rates.展开更多
文摘Background: Anesthetic agents are commonly utilized in the handling of non‐human primates for prevent the stress caused in physical exploration or physical restrain. For this reason, the objective of this work was to describe the effect of age and dissociative anesthetics (ketamine and tiletamine), and their combinations with acepromazine, xylazine and zolazepam, on the physiological and blood biochemical parameters in Macaca mulatta. Methods: Eighty male Macaca mulatta were divided into four experimental groups depending on the anesthetic mixture applied. Each group of 20 males was divided into five sub‐groups according to age. Physiological parameters were recorded every five minutes during a 30‐minute period. A blood sample was drawn to analyze blood biochemistry. Results: Statistical analyses revealed significant differences in the physiological parameters between the ketamine‐acepromazine and ketamine‐xylazine groups compared to the control group. The analysis of blood biochemistry found significant differences by age and by anesthetic mixture among all groups. Conclusion: These findings contribute to standardizing this animal model in biological research.
文摘Targeted protein degradation (TPD) revolutionizes drug discovery by taking advantages of event-driven mechanism of action (MOA). Among current proximity-inducing platforms for TPD, molecular glue degraders and proteolysis-targeting chimeras (PROTACs) are representative strategies that facilitate interactions between an E3 ligase and a specific protein target. This leads to ubiquitination and subsequent degradation of the target by the ubiquitin–proteasome system (UPS). Despite offering unprecedented opportunities for more effective and targeted therapies, current UPS-hijacking TPD still faces key challenges. For instance, although over 600 E3 ligases have been identified in human cells, only a handful, such as cereblon (CRBN) and von Hippel-Lindau (VHL), are accessible for developing degraders1. In addition, there are ongoing questions about how to further expand the chemically tractable target space beyond monomeric proteins with cytosolic domains.
文摘The present study was conduct to compare different oral administration of medication to induce sedation in dogs. In the domestic dogs,the drugs of tiletamine-zolazepam and acepromazine (TZA) through oral administration were used to induce sedation in dogs. Three administration methods were in order directly feeding liquid drugs,encapsulated drugs and liquid drugs mixed with cooked gravy. In the domestic dogs (n = 6),the results showed that the time to sedation score of 4 (sternal recumbency,reluctant to stand) and the capture ratio of dogs in three administration methods were 23 ± 25 min and 83%,27 ± 20 min and 83%,56 ± 27 min and 100%. The time to sedation score of 5 (lateral recumbency,unable to maintain sternal recumbency) and the capture ratio of dogs in three administration methods were 53 ± 22 min and 67%,32 ± 20 min and 83%,730 ± 25 min and 100%,respectively. In the field dogs,17 of 27 dogs feed the TZA drug mixed with cooked gravy or packaged into aspic achieved sedation score of 4 and further successfully captured. In conclusion,the drugs of tiletamine-zolazepam and acepromazine (TZA) through oral administration could be safely and successfully captured the dog in field,and furthermore the palatability of drugs would apparently affect the capturing rates.
