Nitrogen narcosis is a neurological syndrome that manifests when humans or animals encounter hyperbaric nitrogen,resulting in a range of motor,emotional,and cognitive abnormalities.The anterior cingulate cortex(ACC)is...Nitrogen narcosis is a neurological syndrome that manifests when humans or animals encounter hyperbaric nitrogen,resulting in a range of motor,emotional,and cognitive abnormalities.The anterior cingulate cortex(ACC)is known for its significant involvement in regulating motivation,cognition,and action.However,its specific contribution to nitrogen narcosis-induced hyperlocomotion and the underlying mechanisms remain poorly understood.Here we report that exposure to hyperbaric nitrogen notably increased the locomotor activity of mice in a pressure-dependent manner.Concurrently,this exposure induced heightened activation among neurons in both the ACC and dorsal medial striatum(DMS).Notably,chemogenetic inhibition of ACC neurons effectively suppressed hyperlocomotion.Conversely,chemogenetic excitation lowered the hyperbaric pressure threshold required to induce hyperlocomotion.Moreover,both chemogenetic inhibition and genetic ablation of activity-dependent neurons within the ACC reduced the hyperlocomotion.Further investigation revealed that ACC neurons project to the DMS,and chemogenetic inhibition of ACC-DMS projections resulted in a reduction in hyperlocomotion.Finally,nitrogen narcosis led to an increase in local field potentials in the theta frequency band and a decrease in the alpha frequency band in both the ACC and DMS.These results collectively suggest that excitatory neurons within the ACC,along with their projections to the DMS,play a pivotal role in regulating the hyperlocomotion induced by exposure to hyperbaric nitrogen.展开更多
基金supported by the National Natural Science Foundation of China(32030048,82271256,and 32171000)the Natural Science Fund for Colleges and Universities in Jiangsu Province(22KJD340001)+1 种基金the Natural Science Foundation of Jiangsu Province(BK20211107)the Scientific Research Project of"226 Engineering"of Nantong Municipality(2020-9).
文摘Nitrogen narcosis is a neurological syndrome that manifests when humans or animals encounter hyperbaric nitrogen,resulting in a range of motor,emotional,and cognitive abnormalities.The anterior cingulate cortex(ACC)is known for its significant involvement in regulating motivation,cognition,and action.However,its specific contribution to nitrogen narcosis-induced hyperlocomotion and the underlying mechanisms remain poorly understood.Here we report that exposure to hyperbaric nitrogen notably increased the locomotor activity of mice in a pressure-dependent manner.Concurrently,this exposure induced heightened activation among neurons in both the ACC and dorsal medial striatum(DMS).Notably,chemogenetic inhibition of ACC neurons effectively suppressed hyperlocomotion.Conversely,chemogenetic excitation lowered the hyperbaric pressure threshold required to induce hyperlocomotion.Moreover,both chemogenetic inhibition and genetic ablation of activity-dependent neurons within the ACC reduced the hyperlocomotion.Further investigation revealed that ACC neurons project to the DMS,and chemogenetic inhibition of ACC-DMS projections resulted in a reduction in hyperlocomotion.Finally,nitrogen narcosis led to an increase in local field potentials in the theta frequency band and a decrease in the alpha frequency band in both the ACC and DMS.These results collectively suggest that excitatory neurons within the ACC,along with their projections to the DMS,play a pivotal role in regulating the hyperlocomotion induced by exposure to hyperbaric nitrogen.
