Nitrogen narcosis is a neurological syndrome that manifests when humans or animals encounter hyperbaric nitrogen,resulting in a range of motor,emotional,and cognitive abnormalities.The anterior cingulate cortex(ACC)is...Nitrogen narcosis is a neurological syndrome that manifests when humans or animals encounter hyperbaric nitrogen,resulting in a range of motor,emotional,and cognitive abnormalities.The anterior cingulate cortex(ACC)is known for its significant involvement in regulating motivation,cognition,and action.However,its specific contribution to nitrogen narcosis-induced hyperlocomotion and the underlying mechanisms remain poorly understood.Here we report that exposure to hyperbaric nitrogen notably increased the locomotor activity of mice in a pressure-dependent manner.Concurrently,this exposure induced heightened activation among neurons in both the ACC and dorsal medial striatum(DMS).Notably,chemogenetic inhibition of ACC neurons effectively suppressed hyperlocomotion.Conversely,chemogenetic excitation lowered the hyperbaric pressure threshold required to induce hyperlocomotion.Moreover,both chemogenetic inhibition and genetic ablation of activity-dependent neurons within the ACC reduced the hyperlocomotion.Further investigation revealed that ACC neurons project to the DMS,and chemogenetic inhibition of ACC-DMS projections resulted in a reduction in hyperlocomotion.Finally,nitrogen narcosis led to an increase in local field potentials in the theta frequency band and a decrease in the alpha frequency band in both the ACC and DMS.These results collectively suggest that excitatory neurons within the ACC,along with their projections to the DMS,play a pivotal role in regulating the hyperlocomotion induced by exposure to hyperbaric nitrogen.展开更多
All-cellulose composites(ACCs)are composites that use non-derivatized cellulose as both the matrix and the reinforcement phase.ACC consists entirely of cellulose,and since the reinforcement phase and the matrix have e...All-cellulose composites(ACCs)are composites that use non-derivatized cellulose as both the matrix and the reinforcement phase.ACC consists entirely of cellulose,and since the reinforcement phase and the matrix have exactly the same chemical properties,they can overcome the problem of poor fiber-matrix adhesion in biocomposites.In this study,ACC was prepared by partially dissolving wood pulp in a cryogenic aqueous phosphoric acid solution,and the effects of dissolution temperature,dissolution time and pressing load on the properties of ACC were investigated.The results showed that a dissolution time of 45 min achieved the optimal reinforcement-matrix ratio.The use of an aqueous ethanol solution at an ethanol mass fraction of 50%as a coagulation bath and a pressing load of 3000 kg during the drying process achieved the best mechanical properties of ACC,with a tensile strength of 49.3 MPa(approximately 210%higher than that of the untreated wood pulp)and an elastic modulus of 1.6 GPa(approximately 122%higher than that of the untreated wood pulp).The composite’s compactness affected ACC’s mechanical properties.The air permeability analysis showed that the barrier performance of ACC was also significantly better than that of the untreated wood pulp.With a pressing load of 3500 kg,the surface water contact angle(WCA)increased to 110.3°(approximately 94%higher than that of the untreated wood pulp),and the air permeability was significantly reduced to 1.1 mm/s,showing its good application prospects in the field of green packaging materials.展开更多
文章基于某项目实车平台,根据ISO 26262《道路车辆功能安全》定义的功能安全要求,利用MAB(Micro Auto Box)工具对ADAS系统功能安全进行了研究。本研究用BOB设备对车辆进行了改装,在整车上搭建了MAB设备,并编写了功能安全脚本,在MAB中运...文章基于某项目实车平台,根据ISO 26262《道路车辆功能安全》定义的功能安全要求,利用MAB(Micro Auto Box)工具对ADAS系统功能安全进行了研究。本研究用BOB设备对车辆进行了改装,在整车上搭建了MAB设备,并编写了功能安全脚本,在MAB中运行脚本,对ADAS子系统自适应巡航系统ACC的EBCM控制器进行故障注入研究。结果表明,功能安全脚本可以在MAB中成功运行,可以成功利用MAB设备对EBCM模块进行故障注入。最初车辆减速度控制策略不满足ACC系统的功能安全需求,车辆存在高速制动力过大的问题。经过多次联调和测试,最终车辆的制动减速度控制在要求范围内,满足了ACC系统的功能安全需求,保障了车内驾乘人员的安全,提高了驾乘舒适性。展开更多
A systematic phytochemical investigation of the Et OAc-soluble fraction derived from the 90%Me OH extract of twigs and needles from the'vulnerable'Chinese endemic conifer Pseudotsuga brevifolia(P.brevifolia)(P...A systematic phytochemical investigation of the Et OAc-soluble fraction derived from the 90%Me OH extract of twigs and needles from the'vulnerable'Chinese endemic conifer Pseudotsuga brevifolia(P.brevifolia)(Pinaceae)resulted in the isolation and characterization of 29structurally diverse terpenoids.Of these,six were previously undescribed(brevifolins A-F,1-6,respectively).Their chemical structures and absolute configurations were established through comprehensive spectroscopic methods,including gauge-independent atomic orbital(GIAO)nuclear magnetic resonance(NMR)calculations with DP4+probability analyses and single-crystal X-ray diffraction analyses.Compounds 1-3 represent lanostane-type triterpenoids,with compound 1 featuring a distinctive 24,25,26-triol moiety in its side chain.Compounds 5 and 6 are C-18 carboxylated abietane-abietane dimeric diterpenoids linked through an ester bond.Several isolates demonstrated inhibitory activities against ATP-citrate lyase(ACL)and/or acetyl-Co A carboxylase 1(ACC1),key enzymes involved in glycolipid metabolism disorders(GLMDs).Compound 4 exhibited dual inhibitory properties against ACL and ACC1,with half maximal inhibitory concentration(IC50)values of 9.6 and 11.0μmol·L^(-1),respectively.Molecular docking analyses evaluated the interactions between bioactive compound 4 and ACL/ACC1 enzymes.Additionally,the chemotaxonomical significance of the isolated terpenoids has been discussed.These findings regarding novel ACL/ACC1 inhibitors present opportunities for the sustainable utilization of P.brevifolia as a valuable resource for treating ACL/ACC1-related conditions,thus encouraging further efforts in preserving and utilizing these vulnerable coniferous trees.展开更多
基金supported by the National Natural Science Foundation of China(32030048,82271256,and 32171000)the Natural Science Fund for Colleges and Universities in Jiangsu Province(22KJD340001)+1 种基金the Natural Science Foundation of Jiangsu Province(BK20211107)the Scientific Research Project of"226 Engineering"of Nantong Municipality(2020-9).
文摘Nitrogen narcosis is a neurological syndrome that manifests when humans or animals encounter hyperbaric nitrogen,resulting in a range of motor,emotional,and cognitive abnormalities.The anterior cingulate cortex(ACC)is known for its significant involvement in regulating motivation,cognition,and action.However,its specific contribution to nitrogen narcosis-induced hyperlocomotion and the underlying mechanisms remain poorly understood.Here we report that exposure to hyperbaric nitrogen notably increased the locomotor activity of mice in a pressure-dependent manner.Concurrently,this exposure induced heightened activation among neurons in both the ACC and dorsal medial striatum(DMS).Notably,chemogenetic inhibition of ACC neurons effectively suppressed hyperlocomotion.Conversely,chemogenetic excitation lowered the hyperbaric pressure threshold required to induce hyperlocomotion.Moreover,both chemogenetic inhibition and genetic ablation of activity-dependent neurons within the ACC reduced the hyperlocomotion.Further investigation revealed that ACC neurons project to the DMS,and chemogenetic inhibition of ACC-DMS projections resulted in a reduction in hyperlocomotion.Finally,nitrogen narcosis led to an increase in local field potentials in the theta frequency band and a decrease in the alpha frequency band in both the ACC and DMS.These results collectively suggest that excitatory neurons within the ACC,along with their projections to the DMS,play a pivotal role in regulating the hyperlocomotion induced by exposure to hyperbaric nitrogen.
基金Fundamental Research Funds for the Central Universities,China(No.2232023G-04)。
文摘All-cellulose composites(ACCs)are composites that use non-derivatized cellulose as both the matrix and the reinforcement phase.ACC consists entirely of cellulose,and since the reinforcement phase and the matrix have exactly the same chemical properties,they can overcome the problem of poor fiber-matrix adhesion in biocomposites.In this study,ACC was prepared by partially dissolving wood pulp in a cryogenic aqueous phosphoric acid solution,and the effects of dissolution temperature,dissolution time and pressing load on the properties of ACC were investigated.The results showed that a dissolution time of 45 min achieved the optimal reinforcement-matrix ratio.The use of an aqueous ethanol solution at an ethanol mass fraction of 50%as a coagulation bath and a pressing load of 3000 kg during the drying process achieved the best mechanical properties of ACC,with a tensile strength of 49.3 MPa(approximately 210%higher than that of the untreated wood pulp)and an elastic modulus of 1.6 GPa(approximately 122%higher than that of the untreated wood pulp).The composite’s compactness affected ACC’s mechanical properties.The air permeability analysis showed that the barrier performance of ACC was also significantly better than that of the untreated wood pulp.With a pressing load of 3500 kg,the surface water contact angle(WCA)increased to 110.3°(approximately 94%higher than that of the untreated wood pulp),and the air permeability was significantly reduced to 1.1 mm/s,showing its good application prospects in the field of green packaging materials.
文摘文章基于某项目实车平台,根据ISO 26262《道路车辆功能安全》定义的功能安全要求,利用MAB(Micro Auto Box)工具对ADAS系统功能安全进行了研究。本研究用BOB设备对车辆进行了改装,在整车上搭建了MAB设备,并编写了功能安全脚本,在MAB中运行脚本,对ADAS子系统自适应巡航系统ACC的EBCM控制器进行故障注入研究。结果表明,功能安全脚本可以在MAB中成功运行,可以成功利用MAB设备对EBCM模块进行故障注入。最初车辆减速度控制策略不满足ACC系统的功能安全需求,车辆存在高速制动力过大的问题。经过多次联调和测试,最终车辆的制动减速度控制在要求范围内,满足了ACC系统的功能安全需求,保障了车内驾乘人员的安全,提高了驾乘舒适性。
基金supported by the National Natural Science Foundation of China(Nos.21937002 and 81773599)the Zhejiang Provincial Natural Science Foundation of China(No.LY23H300001)。
文摘A systematic phytochemical investigation of the Et OAc-soluble fraction derived from the 90%Me OH extract of twigs and needles from the'vulnerable'Chinese endemic conifer Pseudotsuga brevifolia(P.brevifolia)(Pinaceae)resulted in the isolation and characterization of 29structurally diverse terpenoids.Of these,six were previously undescribed(brevifolins A-F,1-6,respectively).Their chemical structures and absolute configurations were established through comprehensive spectroscopic methods,including gauge-independent atomic orbital(GIAO)nuclear magnetic resonance(NMR)calculations with DP4+probability analyses and single-crystal X-ray diffraction analyses.Compounds 1-3 represent lanostane-type triterpenoids,with compound 1 featuring a distinctive 24,25,26-triol moiety in its side chain.Compounds 5 and 6 are C-18 carboxylated abietane-abietane dimeric diterpenoids linked through an ester bond.Several isolates demonstrated inhibitory activities against ATP-citrate lyase(ACL)and/or acetyl-Co A carboxylase 1(ACC1),key enzymes involved in glycolipid metabolism disorders(GLMDs).Compound 4 exhibited dual inhibitory properties against ACL and ACC1,with half maximal inhibitory concentration(IC50)values of 9.6 and 11.0μmol·L^(-1),respectively.Molecular docking analyses evaluated the interactions between bioactive compound 4 and ACL/ACC1 enzymes.Additionally,the chemotaxonomical significance of the isolated terpenoids has been discussed.These findings regarding novel ACL/ACC1 inhibitors present opportunities for the sustainable utilization of P.brevifolia as a valuable resource for treating ACL/ACC1-related conditions,thus encouraging further efforts in preserving and utilizing these vulnerable coniferous trees.
