A cDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of m...A cDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of maize Call gene. Three truncated overlapping positive clones designated ABP9 were obtained and the full-length cDNA was isolated by 5’ RACE. Searching the database revealed that ABP9 protein belongs to a bZIP-type transcription factor family. ABP9 protein speciflcally binds to ABRE2 motif and activates the expression of downstream reporter gene in yeast cells. Our results strongly suggest that the ABP9 protein functions as a transcription activator.展开更多
目的分析儿童复发性眩晕(recurrent vertigo of children,RVC)患儿的各项相关客观检查结果,试图寻找出有意义的客观检查方法。方法选取RVC患儿50例(眩晕发作组29例,眩晕不发作组21例)和非RVC正常对照组儿童20例,所有入组人员行相关客观...目的分析儿童复发性眩晕(recurrent vertigo of children,RVC)患儿的各项相关客观检查结果,试图寻找出有意义的客观检查方法。方法选取RVC患儿50例(眩晕发作组29例,眩晕不发作组21例)和非RVC正常对照组儿童20例,所有入组人员行相关客观检查,包括位置试验、vHIT、头颅MRI、纯音测听或游戏测听、声导抗、高刺激率ABR、脑电图、脉搏氧及PSG检查,分析各项检查结果,比较RVC组及对照组之间的特征性差异。结果①RVC组脉搏氧异常率、高刺激率ABR异常率及AHI异常率均高于对照组,有统计学差异(均为P<0.05);②眩晕发作组脉搏氧异常率、高刺激率ABR异常率高于眩晕不发作组,有统计学差异(P<0.05);③RVC组和正常对照组的纯音听阈(或游戏测听)、声导抗、颅脑MRI、位置试验检查、vHIT均正常。结论连续睡眠脉搏氧、高刺激率ABR与RVC,尤其是眩晕发作期的RVC具有一定的相关性。AHI与RVC有一定相关性,但与眩晕是否处于发作期没有相关性。连续睡眠脉搏氧监测、PSG、高刺激率ABR可作为诊断RVC的辅助检查。展开更多
基金This work was supported by the National Key Basic Research Program (Grant No. G1999011703)the National Trans-genie Plants Research and Commercialization Program (Grant No. J99-A-028).
文摘A cDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of maize Call gene. Three truncated overlapping positive clones designated ABP9 were obtained and the full-length cDNA was isolated by 5’ RACE. Searching the database revealed that ABP9 protein belongs to a bZIP-type transcription factor family. ABP9 protein speciflcally binds to ABRE2 motif and activates the expression of downstream reporter gene in yeast cells. Our results strongly suggest that the ABP9 protein functions as a transcription activator.
文摘目的分析儿童复发性眩晕(recurrent vertigo of children,RVC)患儿的各项相关客观检查结果,试图寻找出有意义的客观检查方法。方法选取RVC患儿50例(眩晕发作组29例,眩晕不发作组21例)和非RVC正常对照组儿童20例,所有入组人员行相关客观检查,包括位置试验、vHIT、头颅MRI、纯音测听或游戏测听、声导抗、高刺激率ABR、脑电图、脉搏氧及PSG检查,分析各项检查结果,比较RVC组及对照组之间的特征性差异。结果①RVC组脉搏氧异常率、高刺激率ABR异常率及AHI异常率均高于对照组,有统计学差异(均为P<0.05);②眩晕发作组脉搏氧异常率、高刺激率ABR异常率高于眩晕不发作组,有统计学差异(P<0.05);③RVC组和正常对照组的纯音听阈(或游戏测听)、声导抗、颅脑MRI、位置试验检查、vHIT均正常。结论连续睡眠脉搏氧、高刺激率ABR与RVC,尤其是眩晕发作期的RVC具有一定的相关性。AHI与RVC有一定相关性,但与眩晕是否处于发作期没有相关性。连续睡眠脉搏氧监测、PSG、高刺激率ABR可作为诊断RVC的辅助检查。
