A cDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of m...A cDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of maize Call gene. Three truncated overlapping positive clones designated ABP9 were obtained and the full-length cDNA was isolated by 5’ RACE. Searching the database revealed that ABP9 protein belongs to a bZIP-type transcription factor family. ABP9 protein speciflcally binds to ABRE2 motif and activates the expression of downstream reporter gene in yeast cells. Our results strongly suggest that the ABP9 protein functions as a transcription activator.展开更多
随着视频流量在网络中所占的比重不断增加,用户对高体验质量的需求同步提升。如何在有限的条件下为用户提供高质量的体验服务,成为学界和产业界非常重视的问题。自适应比特率(Adaptive Bit Rate,ABR)技术能够使客户端的播放器获得与当...随着视频流量在网络中所占的比重不断增加,用户对高体验质量的需求同步提升。如何在有限的条件下为用户提供高质量的体验服务,成为学界和产业界非常重视的问题。自适应比特率(Adaptive Bit Rate,ABR)技术能够使客户端的播放器获得与当前网络带宽相匹配的视频片段,是目前网络视频传输普遍采用的技术。基于此,分析ABR技术的架构,将其分为基于带宽的算法、基于缓冲区的算法和基于体验质量(Quality of Experience,Qo E)的算法,分别介绍相应的代表算法,并总结这些算法各自的优缺点和适用场景。总结现有算法存在的问题,并提出未来可能的研究方向,希望能够为该领域的研究提供参考。展开更多
基金This work was supported by the National Key Basic Research Program (Grant No. G1999011703)the National Trans-genie Plants Research and Commercialization Program (Grant No. J99-A-028).
文摘A cDNA library was constructed using mRNA extracted from 17 days post-pollination (dpp) maize embryos and was screened by employing a yeast one-hybrid system for proteins specifically interacting with ABRE2 motif of maize Call gene. Three truncated overlapping positive clones designated ABP9 were obtained and the full-length cDNA was isolated by 5’ RACE. Searching the database revealed that ABP9 protein belongs to a bZIP-type transcription factor family. ABP9 protein speciflcally binds to ABRE2 motif and activates the expression of downstream reporter gene in yeast cells. Our results strongly suggest that the ABP9 protein functions as a transcription activator.
文摘随着视频流量在网络中所占的比重不断增加,用户对高体验质量的需求同步提升。如何在有限的条件下为用户提供高质量的体验服务,成为学界和产业界非常重视的问题。自适应比特率(Adaptive Bit Rate,ABR)技术能够使客户端的播放器获得与当前网络带宽相匹配的视频片段,是目前网络视频传输普遍采用的技术。基于此,分析ABR技术的架构,将其分为基于带宽的算法、基于缓冲区的算法和基于体验质量(Quality of Experience,Qo E)的算法,分别介绍相应的代表算法,并总结这些算法各自的优缺点和适用场景。总结现有算法存在的问题,并提出未来可能的研究方向,希望能够为该领域的研究提供参考。
