Lipids are an important energy source and are utilized as substrates for various physiological processes in insects.Comparative gene identification 58(CGI-58),also known asα/βhydrolase domain-containing 5(ABHD5),is ...Lipids are an important energy source and are utilized as substrates for various physiological processes in insects.Comparative gene identification 58(CGI-58),also known asα/βhydrolase domain-containing 5(ABHD5),is a highly conserved and multifunctional gene involved in regulating lipid metabolism and cellular energy balance in many organisms.However,the biological functions of ABHD5 in insects are poorly understood.In the current study,we describe the identification and characterization of the ABHD5 gene in the lepidopteran model insect,Bombyx mori.The tissue expression profile investigated using quantitative reverse transcription polymerase chain reaction(RT-qPCR)reveals that BmABHD5 is widely expressed in all tissues,with particularly high levels found in the midgut and testis.A binary transgenic CRISPR/Cas9 system was employed to conduct a functional analysis of BmABHD5,with the mutation of BmABHD5 leading to the dysregulation of lipid metabolism and excessive lipid accumulation in the larval midgut.Histological and physiological analysis further reveals a significant accumulation of lipid droplets in the midgut of mutant larvae.RNA-seq and RT-qPCR analysis showed that genes related to metabolic pathways were significantly affected by the absence of BmABHD5.Altogether,our data prove that BmABHD5 plays an important role in regulating tissue-specific lipid metabolism in the silkworm midgut.展开更多
基金supported by the National Science Foundation of China(31925007 and 32000333)the Natural Science Foundation of Jiangsu Province(BK20221292).
文摘Lipids are an important energy source and are utilized as substrates for various physiological processes in insects.Comparative gene identification 58(CGI-58),also known asα/βhydrolase domain-containing 5(ABHD5),is a highly conserved and multifunctional gene involved in regulating lipid metabolism and cellular energy balance in many organisms.However,the biological functions of ABHD5 in insects are poorly understood.In the current study,we describe the identification and characterization of the ABHD5 gene in the lepidopteran model insect,Bombyx mori.The tissue expression profile investigated using quantitative reverse transcription polymerase chain reaction(RT-qPCR)reveals that BmABHD5 is widely expressed in all tissues,with particularly high levels found in the midgut and testis.A binary transgenic CRISPR/Cas9 system was employed to conduct a functional analysis of BmABHD5,with the mutation of BmABHD5 leading to the dysregulation of lipid metabolism and excessive lipid accumulation in the larval midgut.Histological and physiological analysis further reveals a significant accumulation of lipid droplets in the midgut of mutant larvae.RNA-seq and RT-qPCR analysis showed that genes related to metabolic pathways were significantly affected by the absence of BmABHD5.Altogether,our data prove that BmABHD5 plays an important role in regulating tissue-specific lipid metabolism in the silkworm midgut.
