To detect the function of proteolipid protein (PLP) peptide (residues 56-70) sp ecific CD 4 + T cells in experimental allergic encephalomyelitis (EAE) in Bioz zi AB/H mice (H 2A g7 ) Methods Biozzi AB/H mice were immu...To detect the function of proteolipid protein (PLP) peptide (residues 56-70) sp ecific CD 4 + T cells in experimental allergic encephalomyelitis (EAE) in Bioz zi AB/H mice (H 2A g7 ) Methods Biozzi AB/H mice were immunized by synthetic PLP 56 70 peptide (DYEYLINVIH AFQYV) which was emulsified by sonication with complete Freund’s adjuvant, a EAE model proven histologically and clinically Murine splenocytes and spinal cord infiltrated (SCI) T cells were stimulated by PLP 56 70 , then the CD 4 + T cells were isolated by Dynabeads, and confirmed by staining with anti CD 4 antibody Finally, the IL2 bioassay and IFN γ/IL4 ELISA were done to detect T cell proliferation and cytokine secretion after PLP 56 70 stimulation Results The histology of murine spinal cord showed a great number of lymphocytes infiltr ated the spinal cord; the clinical signs showed high scores (4 3) on the peak, as well as a good EAE model After being isolated by Dynabea ds , CD 4 + T cells showed high purification (>99%) by staining with anti CD 4 antibody IL2 bioassay showed that those T cells were PLP 56 70 specific T cells ELISA showed that those T cells had high IFN γ/IL4 ratio , indicating that they are T helper 1 (Th1) cells Conclusions PLP 56 70 specific splenocytes and SCI CD 4 + T cells in EAE from Bioz zi AB/H mice were detected and showed that both of them were P LP 56 70 specific Th1 cells It is beneficial to understand what kind o f role these T cells play in the development of展开更多
生物大分子除脂肪外都是聚合物,它们和晶体类似,具有周期性结构。由于直接计算生物大分子,目前还受计算机容量限制,因而我们采用含 H 杂质的 Li 链模型进行模拟计算。在计算中用到 Hartree-Fock-Roothaan 方程,mulliken 集居数和基函数...生物大分子除脂肪外都是聚合物,它们和晶体类似,具有周期性结构。由于直接计算生物大分子,目前还受计算机容量限制,因而我们采用含 H 杂质的 Li 链模型进行模拟计算。在计算中用到 Hartree-Fock-Roothaan 方程,mulliken 集居数和基函数系。从计算结果看出蛋白质的半导性可能与杂质能级有关。展开更多
The ab initio method has been used to study the 1-3 H transfer reaction on formamidine substituted by halogen. The calculation results show that the substituted halogen has two effects on the 1-3 H transfer reaction...The ab initio method has been used to study the 1-3 H transfer reaction on formamidine substituted by halogen. The calculation results show that the substituted halogen has two effects on the 1-3 H transfer reaction: decreasing the activation energy and stabilizing the C=N double bond owing to the conjugative effect of p-π-p of products and transition states.展开更多
文摘To detect the function of proteolipid protein (PLP) peptide (residues 56-70) sp ecific CD 4 + T cells in experimental allergic encephalomyelitis (EAE) in Bioz zi AB/H mice (H 2A g7 ) Methods Biozzi AB/H mice were immunized by synthetic PLP 56 70 peptide (DYEYLINVIH AFQYV) which was emulsified by sonication with complete Freund’s adjuvant, a EAE model proven histologically and clinically Murine splenocytes and spinal cord infiltrated (SCI) T cells were stimulated by PLP 56 70 , then the CD 4 + T cells were isolated by Dynabeads, and confirmed by staining with anti CD 4 antibody Finally, the IL2 bioassay and IFN γ/IL4 ELISA were done to detect T cell proliferation and cytokine secretion after PLP 56 70 stimulation Results The histology of murine spinal cord showed a great number of lymphocytes infiltr ated the spinal cord; the clinical signs showed high scores (4 3) on the peak, as well as a good EAE model After being isolated by Dynabea ds , CD 4 + T cells showed high purification (>99%) by staining with anti CD 4 antibody IL2 bioassay showed that those T cells were PLP 56 70 specific T cells ELISA showed that those T cells had high IFN γ/IL4 ratio , indicating that they are T helper 1 (Th1) cells Conclusions PLP 56 70 specific splenocytes and SCI CD 4 + T cells in EAE from Bioz zi AB/H mice were detected and showed that both of them were P LP 56 70 specific Th1 cells It is beneficial to understand what kind o f role these T cells play in the development of
文摘生物大分子除脂肪外都是聚合物,它们和晶体类似,具有周期性结构。由于直接计算生物大分子,目前还受计算机容量限制,因而我们采用含 H 杂质的 Li 链模型进行模拟计算。在计算中用到 Hartree-Fock-Roothaan 方程,mulliken 集居数和基函数系。从计算结果看出蛋白质的半导性可能与杂质能级有关。
基金Supported by the Yunnan Provincial Science and Technology Department (2003A0003M)
文摘The ab initio method has been used to study the 1-3 H transfer reaction on formamidine substituted by halogen. The calculation results show that the substituted halogen has two effects on the 1-3 H transfer reaction: decreasing the activation energy and stabilizing the C=N double bond owing to the conjugative effect of p-π-p of products and transition states.
