Metabolites in skeletal muscles play an important role in their growth,development,immunity and other physiological activities.However,the genetic basis of metabolites in skeletal muscle remains poorly understood.Here...Metabolites in skeletal muscles play an important role in their growth,development,immunity and other physiological activities.However,the genetic basis of metabolites in skeletal muscle remains poorly understood.Here,we identified 247 candidate divergent regions containing 905 protein-coding genes closely related to metabolic pathways,including lysine degradation and fatty acid biosynthesis.We then profiled 3,060 metabolites in 246 skeletal muscle samples from F_2 segregating population generated by mallard×Pekin duck crosses using metabolomic approaches.We identified 2,044 significant metabolome-based GWAS signals and 21 candidate genes potentially modulating metabolite contents in skeletal muscle.Among them,the levels of 2-aminoadipic acid in skeletal muscle were significantly correlated with body weight and intramuscular fat content,determined by a 939-bp CR1 LINE insertion in AADAT.We further found that the CR1 LINE insertion most possibly led to a splice mutation in AADAT,resulting in the downregulation of the lysine degradation pathway in skeletal muscle.Moreover,intramuscular fat content and fatty acids biosynthesis pathway was significantly increased in individuals with CR1 LINE insertion.This study enhances our understanding of the genetic basis of skeletal muscle metabolic traits and promotes the efficient utilization of metabolite traits in the genetic improvement of animals.展开更多
基金supported by grants from the National Science Fund for Distinguished Young Scholars (32325047)the Innovation Program of Chinese Academy of Agricultural Sciences (CAAS-CS-202201)+1 种基金the China Agriculture Research System of MOF and MARA (CARS-42-05)the National Key R&D Program of China (2023YFD1300300,2022YFF1000100)。
文摘Metabolites in skeletal muscles play an important role in their growth,development,immunity and other physiological activities.However,the genetic basis of metabolites in skeletal muscle remains poorly understood.Here,we identified 247 candidate divergent regions containing 905 protein-coding genes closely related to metabolic pathways,including lysine degradation and fatty acid biosynthesis.We then profiled 3,060 metabolites in 246 skeletal muscle samples from F_2 segregating population generated by mallard×Pekin duck crosses using metabolomic approaches.We identified 2,044 significant metabolome-based GWAS signals and 21 candidate genes potentially modulating metabolite contents in skeletal muscle.Among them,the levels of 2-aminoadipic acid in skeletal muscle were significantly correlated with body weight and intramuscular fat content,determined by a 939-bp CR1 LINE insertion in AADAT.We further found that the CR1 LINE insertion most possibly led to a splice mutation in AADAT,resulting in the downregulation of the lysine degradation pathway in skeletal muscle.Moreover,intramuscular fat content and fatty acids biosynthesis pathway was significantly increased in individuals with CR1 LINE insertion.This study enhances our understanding of the genetic basis of skeletal muscle metabolic traits and promotes the efficient utilization of metabolite traits in the genetic improvement of animals.
