Acupuncture,a form of traditional Chinese medicine with a history of 2,000 years in China,has gained wider acceptance worldwide as a complementary therapy.Studies have examined its effectiveness in various health cond...Acupuncture,a form of traditional Chinese medicine with a history of 2,000 years in China,has gained wider acceptance worldwide as a complementary therapy.Studies have examined its effectiveness in various health conditions and it is commonly used alongside conventional medical treatments.With the development of artificial intelligence(AI)technology,new possibilities for improving the efficacy and precision of acupuncture have emerged.This study explored the combination of traditional acupuncture and AI technology from three perspectives:acupuncture diagnosis,prescription,and treatment evaluation.The study aimed to provide cutting-edge direction and theoretical assistance for the development of an acupuncture robot.展开更多
In the Medicago genus, triterpenic saponins are bioactive secondary metabolites constitutively synthesized in the aerial and subterranean parts of plants via the isoprenoid pathway. Exploitation of saponins as pharmac...In the Medicago genus, triterpenic saponins are bioactive secondary metabolites constitutively synthesized in the aerial and subterranean parts of plants via the isoprenoid pathway. Exploitation of saponins as pharmaceutics, agrochemicals and in the food and cosmetic industries has raised interest in identifying the enzymes involved in their synthesis. We have identified a cytochrome P450 （CYP72A67） involved in hemolytic sapogenin biosynthesis by a reverse genetic TILLING approach in a Medicago truncatula ethyl- methanesulfonate （EMS） mutagenized collection. Genetic and biochemical analyses, mutant complementation, and expression of the gene in a microsome yeast system showed that CYP72A67 is responsible for hydroxylation at the C-2 position downstream of oleanolic acid synthesis. The affinity of CYP72A67 for substrates with different substitutions at multiple carbon positions was investigated in the same in vitro yeast system, and in relation to two other CYP450s （CYP72A68） responsible for the production of medicagenic acid, the main sapogenin in M. truncatula leaves and roots. Full sib mutant and wild-type plants were compared for their sapogenin profile, expression patterns of the genes involved in sapogenin synthesis, and response to inoculation with Sinorhizobium meliloti. The results obtained allowed us to revise the hemolytic sapogenin pathway in M. truncatula and contribute to highlighting the tissue specificities （leaves/roots） of sapogenin synthesis.展开更多
基金supported by the National Natural Science Foundation of China (No.82305376)2021 Special Research Project of TCM Science and Technology Development Plan of Jiangsu Province (ZT202120)+1 种基金Luo Linxiu Teacher Development Funding Project (LLX202308)National Key Research and Development Plan Intelligent Robot (2022YFB4703100).
文摘Acupuncture,a form of traditional Chinese medicine with a history of 2,000 years in China,has gained wider acceptance worldwide as a complementary therapy.Studies have examined its effectiveness in various health conditions and it is commonly used alongside conventional medical treatments.With the development of artificial intelligence(AI)technology,new possibilities for improving the efficacy and precision of acupuncture have emerged.This study explored the combination of traditional acupuncture and AI technology from three perspectives:acupuncture diagnosis,prescription,and treatment evaluation.The study aimed to provide cutting-edge direction and theoretical assistance for the development of an acupuncture robot.
文摘In the Medicago genus, triterpenic saponins are bioactive secondary metabolites constitutively synthesized in the aerial and subterranean parts of plants via the isoprenoid pathway. Exploitation of saponins as pharmaceutics, agrochemicals and in the food and cosmetic industries has raised interest in identifying the enzymes involved in their synthesis. We have identified a cytochrome P450 （CYP72A67） involved in hemolytic sapogenin biosynthesis by a reverse genetic TILLING approach in a Medicago truncatula ethyl- methanesulfonate （EMS） mutagenized collection. Genetic and biochemical analyses, mutant complementation, and expression of the gene in a microsome yeast system showed that CYP72A67 is responsible for hydroxylation at the C-2 position downstream of oleanolic acid synthesis. The affinity of CYP72A67 for substrates with different substitutions at multiple carbon positions was investigated in the same in vitro yeast system, and in relation to two other CYP450s （CYP72A68） responsible for the production of medicagenic acid, the main sapogenin in M. truncatula leaves and roots. Full sib mutant and wild-type plants were compared for their sapogenin profile, expression patterns of the genes involved in sapogenin synthesis, and response to inoculation with Sinorhizobium meliloti. The results obtained allowed us to revise the hemolytic sapogenin pathway in M. truncatula and contribute to highlighting the tissue specificities （leaves/roots） of sapogenin synthesis.
