The anaerobic/anoxic/oxic（A2O） process is globally one of the widely used biological sewage treatment processes. This is the first report of a metagenomic analysis using Illumina sequencing of full-scale A2O sludge ...The anaerobic/anoxic/oxic（A2O） process is globally one of the widely used biological sewage treatment processes. This is the first report of a metagenomic analysis using Illumina sequencing of full-scale A2O sludge from a municipal sewage treatment plant.With more than 530,000 clean reads from different taxa and metabolic categories, the metagenome results allow us to gain insight into the functioning of the biological community of the A2O sludge. There are 51 phyla and nearly 900 genera identified from the A2O activated sludge ecosystem. Proteobacteria, Bacteroidetes, Nitrospirae and Chloroflexi are predominant phyla in the activated sludge, suggesting that these organisms play key roles in the biodegradation processes in the A2O sewage treatment system.Nitrospira, Thauera, Dechloromonas and Ignavibacterium, which have abilities to metabolize nitrogen and aromatic compounds, are most prevalent genera. The percent of nitrogen and phosphorus metabolism in the A2O sludge is 2.72% and 1.48%, respectively. In the current A2O sludge, the proportion of Candidatus Accumulibacter is 1.37%, which is several times more than that reported in a recent study of A2O sludge. Among the four processes of nitrogen metabolism, denitrification related genes had the highest number of sequences（76.74%）, followed by ammonification（15.77%）, nitrogen fixation（3.88%） and nitrification（3.61%）. In phylum Planctomycetes, four genera（Planctomyces, Pirellula, Gemmata and Singulisphaera） are included in the top 30 abundant genera, suggesting the key role of ANAMMOX in nitrogen metabolism in the A2O sludge.展开更多
以上海市某污水处理厂扩建工程项目为背景,分析了该污水处理厂的工艺流程并重点阐述了生物反应池这一核心工艺环节,开发了一套倒置A2O工艺的污水处理SCADA系统,在传统厌氧-缺氧-好氧(A2O)工艺的基础上设立预缺氧池并加置两个缺氧-好氧(...以上海市某污水处理厂扩建工程项目为背景,分析了该污水处理厂的工艺流程并重点阐述了生物反应池这一核心工艺环节,开发了一套倒置A2O工艺的污水处理SCADA系统,在传统厌氧-缺氧-好氧(A2O)工艺的基础上设立预缺氧池并加置两个缺氧-好氧(AO)池。SCADA系统的下位机选用Rockwell Control Logix系列以及Siemens S7-1200的PLC,人机界面采用组态软件KingSCADA3.7进行开发。整个自控系统通过工艺流程模块进行画面实时监控,可以实时记录运行参数、显示参数趋势曲线等。运行结果表明,该污水处理自控系统实现了污水处理厂的集散控制,满足工艺要求,提升了脱氮除磷效率和出水水质。展开更多
基金supported by the National Natural Science Foundation of China (No. 41430643)
文摘The anaerobic/anoxic/oxic（A2O） process is globally one of the widely used biological sewage treatment processes. This is the first report of a metagenomic analysis using Illumina sequencing of full-scale A2O sludge from a municipal sewage treatment plant.With more than 530,000 clean reads from different taxa and metabolic categories, the metagenome results allow us to gain insight into the functioning of the biological community of the A2O sludge. There are 51 phyla and nearly 900 genera identified from the A2O activated sludge ecosystem. Proteobacteria, Bacteroidetes, Nitrospirae and Chloroflexi are predominant phyla in the activated sludge, suggesting that these organisms play key roles in the biodegradation processes in the A2O sewage treatment system.Nitrospira, Thauera, Dechloromonas and Ignavibacterium, which have abilities to metabolize nitrogen and aromatic compounds, are most prevalent genera. The percent of nitrogen and phosphorus metabolism in the A2O sludge is 2.72% and 1.48%, respectively. In the current A2O sludge, the proportion of Candidatus Accumulibacter is 1.37%, which is several times more than that reported in a recent study of A2O sludge. Among the four processes of nitrogen metabolism, denitrification related genes had the highest number of sequences（76.74%）, followed by ammonification（15.77%）, nitrogen fixation（3.88%） and nitrification（3.61%）. In phylum Planctomycetes, four genera（Planctomyces, Pirellula, Gemmata and Singulisphaera） are included in the top 30 abundant genera, suggesting the key role of ANAMMOX in nitrogen metabolism in the A2O sludge.
文摘以上海市某污水处理厂扩建工程项目为背景,分析了该污水处理厂的工艺流程并重点阐述了生物反应池这一核心工艺环节,开发了一套倒置A2O工艺的污水处理SCADA系统,在传统厌氧-缺氧-好氧(A2O)工艺的基础上设立预缺氧池并加置两个缺氧-好氧(AO)池。SCADA系统的下位机选用Rockwell Control Logix系列以及Siemens S7-1200的PLC,人机界面采用组态软件KingSCADA3.7进行开发。整个自控系统通过工艺流程模块进行画面实时监控,可以实时记录运行参数、显示参数趋势曲线等。运行结果表明,该污水处理自控系统实现了污水处理厂的集散控制,满足工艺要求,提升了脱氮除磷效率和出水水质。
