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玉米自交系A28的选育应用及体会 被引量:4
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作者 郑丽敏 牛永锋 +4 位作者 王海莉 白峰 崔俊明 裴振群 刘智萍 《陕西农业科学》 2008年第5期73-76,共4页
A28(420)是由安阳市农科所运用相互交替回交亲本的育种技术于1999年育成的新自交系。我们先后用A28(420)组配安玉12、安玉13两个玉米杂交种通过省级及国家审定。该自交系选育过程中选用了高起点基础材料,采取了异地加代、早代测定、加... A28(420)是由安阳市农科所运用相互交替回交亲本的育种技术于1999年育成的新自交系。我们先后用A28(420)组配安玉12、安玉13两个玉米杂交种通过省级及国家审定。该自交系选育过程中选用了高起点基础材料,采取了异地加代、早代测定、加大选择压力、自交系选育和抗病性鉴定相结合等方法,保证了该自交系的优良性状和高配合力。 展开更多
关键词 玉米 自交系 a28 选育
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线粒体铁转运蛋白2(mitoferrin 2/SLC25A28)的原核表达及兔多克隆抗体制备 被引量:1
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作者 刘磊 聂雅婷 +3 位作者 丁兰 谷雨 袁媛 叶菁 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2023年第3期268-274,共7页
目的构建人线粒体铁转运蛋白2(mitoferrin 2/SLC25A28)的原核表达质粒,进行蛋白表达和纯化,制备兔抗多克隆抗体并进行初步鉴定。方法构建pET28a(+)-SLC25A28-His原核表达质粒,转化至BL21(DE3)大肠杆菌,采用异丙基β-D-硫代半乳糖苷(IPTG... 目的构建人线粒体铁转运蛋白2(mitoferrin 2/SLC25A28)的原核表达质粒,进行蛋白表达和纯化,制备兔抗多克隆抗体并进行初步鉴定。方法构建pET28a(+)-SLC25A28-His原核表达质粒,转化至BL21(DE3)大肠杆菌,采用异丙基β-D-硫代半乳糖苷(IPTG)诱导蛋白表达。提取蛋白包涵体,并用8 mol/L尿素溶解,His-NTA柱纯化。获得纯化SLC25A28蛋白免疫新西兰大白兔制备SLC25A28多克隆抗体,Western blot法鉴定SLC25A28抗体特异性。结果成功构建pET28a(+)-SLC25A28-His原核表达载体质粒,并在BL21(DE3)大肠杆菌中成功诱导表达SLC25A28蛋白,蛋白纯度约为90%,Western blot法证明制备的抗体能特异性识别小鼠睾丸组织中的SLC25A28蛋白。结论成功对人SLC25A28进行了原核表达,并制备了具有特异性的兔抗人SLC25A28多克隆抗体。 展开更多
关键词 线粒体铁转运蛋白2(mitoferrin 2/SLC25a28) 原核表达 蛋白纯化 多克隆抗体 免疫特异性
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床垫变时装 A28携手Sealy”游弋”上海时装周
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《中国标准化》 2016年第10X期12-13,共2页
时装周上,也许你看过模特穿睡衣走T台的,但你一定没见过身着'床垫'走秀的?而就在2017SS这一季的上海时装周里,高级女装成衣品牌A28和全球床垫品牌丝涟让我们见证了'奇迹'.
关键词 时装 a28 Sealy 服装 床垫 时装周 上海
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全金属外壳功放新款上市 Arcam FMJ A28合并式功放
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作者 本刊编辑部 《视听技术》 2009年第7期18-18,共5页
英国Arcam(雅骏)拥有悠久的放大器开发历史,多年来不断为追求完美音质的音乐爱好者们奉献着品质卓越的放大器产品。A28就是Arcam最新为音乐爱好者们奉献的一款中价位产品。A28的造型和外观尺寸与其上级产品A18完全一样,是一款在各方面... 英国Arcam(雅骏)拥有悠久的放大器开发历史,多年来不断为追求完美音质的音乐爱好者们奉献着品质卓越的放大器产品。A28就是Arcam最新为音乐爱好者们奉献的一款中价位产品。A28的造型和外观尺寸与其上级产品A18完全一样,是一款在各方面都非常成熟、充满个性品味的的优质产品。该放大器设计精巧、连接灵活,而且放大级功率达到了75W(8Ω),与A18的50W(8Ω)相比,提升了整整25W,这对于一向稳扎稳打的Arcam而言,已经是一个相当大的提升。该款产品不仅音质出众,而且规格一流。 展开更多
关键词 ARCAM FMJ a28 合并式 放大器设计 音乐爱好者 开发历史 品质卓越 超低噪声 总谐波失真
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DC导购
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《数字通信》 2004年第8期60-65,共6页
来去自如:影像功能必然继彩屏、 和弦之后成为又一手机功能热点。作为国内第一款双摄像头的手机,1小时有声视频连续录制功能再配上64MB超大内存容量,堪称强悍!但天文数字般的上市价,也不是一般人能够接受。
关键词 手机 选购 MOTOA890 KONKA a28 NEC N820
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A 28-Year Climatological Analysis of Size Parameters for Northwestern Pacific Tropical Cyclones 被引量:25
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作者 袁金南 王东晓 +1 位作者 万齐林 刘春霞 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2007年第1期24-34,共11页
A 28-year best track dataset containing size parameters that include the radii of the 15.4 m s^-1 winds (R15) and the 25.7 m s^-1 winds (R26) of tropical cyclones (TCs) in the Northwestern Pacific, the NCEP/ NCA... A 28-year best track dataset containing size parameters that include the radii of the 15.4 m s^-1 winds (R15) and the 25.7 m s^-1 winds (R26) of tropical cyclones (TCs) in the Northwestern Pacific, the NCEP/ NCAR reanalysis dataset and the Extended Reconstructed Sea Surface Temperature (ERSST) dataset are employed in this study. The climatology of size parameters for the tropical cyclones in the Northwestern Pacific from 1977 to 2004 is investigated in terms of the spatial and temporal distributions. The results show that the major activity of TCs in the Northwestern Pacific is from July to October. A majority of TCs lie over the ocean west of 150°E, and a few TCs can intensify to the Saffir-Simpson (S-S) categories 4, 5. Both R15 and R26 tend to increase as the tropical cyclones intensify. The values of R15 and R26 are larger for intense TCs in the Northwestern Pacific than in the North Atlantic generally. Both R15 and R26 peak in October, and before and after October, R15 and R26 decrease, which is different from the case in the North Atlantic. The smaller R15s and R26s occur in a large range over the Northwestern Pacific, while the larger R15s and R26s mainly lie in the eastern ocean from Taiwan Island to the Philippine Islands where many tropical cyclones develop in intense systems. The tropical cyclones with size parameters of R15 or R26 on average take a longer time to intensify than to weaken, and the weak tropical cyclones have faster weakening rates than intensification rates. From 1977 to 2004, the annual mean values of R15 increase basically with year; during the 28-year period, the value of R15 increases by 52.7 kin, but R26 does not change with year obviously. 展开更多
关键词 CLIMATOLOGY size parameters of tropical cyclones Northwestern Pacific a 28-year best track dataset
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Jackson type theorems on complex curves 被引量:3
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作者 TU TianLiang Deptartment of Mathematics and Informatics, North China Institute of Water Conservancy and Hydroelectric Power, Zhengzhou 450011, China 《Science China Mathematics》 SCIE 2009年第3期493-506,共14页
Let Γ be a closed smooth Jordan curve in the complex plane. In this paper, with the help of a class of fundamental functions of Hermite interpolation, the author introduces a continuous function interpolation which u... Let Γ be a closed smooth Jordan curve in the complex plane. In this paper, with the help of a class of fundamental functions of Hermite interpolation, the author introduces a continuous function interpolation which uniformly approximates to f(z) ∈ C(Γ ) with the same order of approximation as that in Jackson Theorem 1 on real interval [1, 1]. The accuracy of the order of approximation is proved. Using the method different from the early works, the author studies simultaneous approximation to function and its derivatives and the desired results analogues to that in Jackson Theorem 2 on real interval [1, 1] are obtained. 展开更多
关键词 complex curves INTERPOLATION uniform approximation order of approximation 30E05 30E10 41A05 41A25 41a28
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