The mortality rate ofAcinetobacter baumannii is as high as 47%,This is inseparable From its strong resistance to carbapenems,polymyxins,aminoglycosides,quinolone antibiotics.This article will analyze the resistance of...The mortality rate ofAcinetobacter baumannii is as high as 47%,This is inseparable From its strong resistance to carbapenems,polymyxins,aminoglycosides,quinolone antibiotics.This article will analyze the resistance of Acinetobacter baumannii to carbapenem antibiotics(meropenem and imipenem)and explore why Acinetobacter baumannii is so resistant.The data I used comes from the NCBI database,which includes six resistant groups(R)to meropenem and imipenem,and five sensitive groups(S).The level of the resistant group is complete genome,three sensitive groups are contig,and two are complete genome.Subsequently,I used Prokka in Galaxy to convert the Fasta.file into various forms such as Faa,gbk,ffn.After performing Fisher's test on all GFF files,I obtained the result chart,which includes the search for relevant domains and protein descriptions on Interpro using the chart.ProteinPlus also performed drug pocket prediction and constructed protein models using Swissmodel,as well as different multi sequence comparisons.Finally,I analyzed the functions of the proteins corresponding to the genes specific to the resistance group and the reasons for the development of drug resistance,making future research on the drug resistance of Acinetobacter baumannii easier.And further research can be conducted on the drug pocket in the protein ofAcinetobacter baumannii that has developed resistance,and drugs can be administered.展开更多
文摘The mortality rate ofAcinetobacter baumannii is as high as 47%,This is inseparable From its strong resistance to carbapenems,polymyxins,aminoglycosides,quinolone antibiotics.This article will analyze the resistance of Acinetobacter baumannii to carbapenem antibiotics(meropenem and imipenem)and explore why Acinetobacter baumannii is so resistant.The data I used comes from the NCBI database,which includes six resistant groups(R)to meropenem and imipenem,and five sensitive groups(S).The level of the resistant group is complete genome,three sensitive groups are contig,and two are complete genome.Subsequently,I used Prokka in Galaxy to convert the Fasta.file into various forms such as Faa,gbk,ffn.After performing Fisher's test on all GFF files,I obtained the result chart,which includes the search for relevant domains and protein descriptions on Interpro using the chart.ProteinPlus also performed drug pocket prediction and constructed protein models using Swissmodel,as well as different multi sequence comparisons.Finally,I analyzed the functions of the proteins corresponding to the genes specific to the resistance group and the reasons for the development of drug resistance,making future research on the drug resistance of Acinetobacter baumannii easier.And further research can be conducted on the drug pocket in the protein ofAcinetobacter baumannii that has developed resistance,and drugs can be administered.
