近日,黄河水利科学研究院(简称黄科院)正式获得ISO/I E C 27001:2022信息安全管理体系(ISMS)和ISO/IEC 20000-1:2018信息技术服务管理体系(ITSMS)认证证书。标志着黄科院在信息安全防护和信息技术服务领域管理水平已达到国际标准,为水...近日,黄河水利科学研究院(简称黄科院)正式获得ISO/I E C 27001:2022信息安全管理体系(ISMS)和ISO/IEC 20000-1:2018信息技术服务管理体系(ITSMS)认证证书。标志着黄科院在信息安全防护和信息技术服务领域管理水平已达到国际标准,为水利信息化业务高质量发展奠定了坚实的管理基础。展开更多
As the only naturally occurring stable derivative of L-ascorbic acid(AA;vitamin C),2-O-β-D-glucopyranosyl-L-ascorbic acid(AA-2βG)is hydrolyzed in vivo to release active AA.AA-2βG exhibits strong antioxidant and ant...As the only naturally occurring stable derivative of L-ascorbic acid(AA;vitamin C),2-O-β-D-glucopyranosyl-L-ascorbic acid(AA-2βG)is hydrolyzed in vivo to release active AA.AA-2βG exhibits strong antioxidant and antiphotoaging effects comparable to those of AA,and it plays a key role in maintaining organismal health.Owing to its superior stability and bioavailability,AA-2βG is considered as a promising,longer-lasting natural alternative to conventional vitamin C.It was first identified and is particularly abundant in Lycii Fructus(Gouqizi in Chinese)but has been detected in several crop plants.This review offers a comprehensive overview of recent advances in AA-2βG research,covering key aspects including discovery,structure,natural sources,extraction and detection methods,chemical and in vitro enzymatic synthesis,biosynthetic pathways,as well as applications in health care,skin care,and functional foods.Additionally,we highlight strategies for leveraging plant resources and enhancing AA-2βG biosynthesis,which are expected to accelerate future research and support the sustainable development and utilization of AA-2βG and other high-value natural products.展开更多
Objectives:The current treatment options and therapeutic targets for triple-negative breast cancer(TNBC),an aggressive subtype of breast cancer(BrCA),are limited.This study aimed to identify novel biomarkers and trans...Objectives:The current treatment options and therapeutic targets for triple-negative breast cancer(TNBC),an aggressive subtype of breast cancer(BrCA),are limited.This study aimed to identify novel biomarkers and transcriptional regulatory networks(TRN)inherent in TNBC samples.Methods:We analyzed pan-cancer BrCA datasets from The Cancer Genome Atlas(TCGA)to compare triple-positive breast cancer(TPBC)with TNBC.TRN algorithms and virtual inference of protein-enriched regulon(VIPER)were used to identify master regulators and their target genes.Utilizing TNBC cells(MDA-MB-231 and MDA-MB-468),we validated the relationship of nuclear factor erythroid 2-like 3(NFE2L3)and basic helix-loop-helix family member E 40(BHLHE40)by performing a luciferase assay.The expression levels of these targets were measured after transfections with plasmid and siRNA via qRT-PCR and western blots.The effect of these genes on cell proliferation and migration was studied using phenotypic assays.Results:Using computational approaches,we identified NFE2L3 as a master regulator with BHLHE40 as its target gene.NFE2L3 protein binds to the promoter region of BHLHE40 and regulates its transcriptional activity.Additionally,silencing and overexpressing NFE2L3 and BHLHE40 in TNBC cell lines MDA-MB-231 and MDA-MB-468 showed that NFE2L3 directly regulates BHLHE40 at both transcriptional and translational levels.We found that BHLHE40 requires NFE2L3 for cell proliferation and migration in TNBC.Conclusion:These findings underscore the significance of NFE2L3 and BHLHE40 in TNBC,highlighting NFE2L3’s role in regulating the oncogenic activity of BHLHE40 in TNBC cells.展开更多
文摘近日,黄河水利科学研究院(简称黄科院)正式获得ISO/I E C 27001:2022信息安全管理体系(ISMS)和ISO/IEC 20000-1:2018信息技术服务管理体系(ITSMS)认证证书。标志着黄科院在信息安全防护和信息技术服务领域管理水平已达到国际标准,为水利信息化业务高质量发展奠定了坚实的管理基础。
基金funded by the National Key Research and Development Program(Nos.2023YFC3504104 and 2024YFD2100700)the Fundamental Research Funds for the Central public welfare research institutes(No.ZZ13-YQ-101)Scientific and technological innovation project of China Academy of Chinese Medical Sciences(No.CI2023E002-Y-28).
文摘As the only naturally occurring stable derivative of L-ascorbic acid(AA;vitamin C),2-O-β-D-glucopyranosyl-L-ascorbic acid(AA-2βG)is hydrolyzed in vivo to release active AA.AA-2βG exhibits strong antioxidant and antiphotoaging effects comparable to those of AA,and it plays a key role in maintaining organismal health.Owing to its superior stability and bioavailability,AA-2βG is considered as a promising,longer-lasting natural alternative to conventional vitamin C.It was first identified and is particularly abundant in Lycii Fructus(Gouqizi in Chinese)but has been detected in several crop plants.This review offers a comprehensive overview of recent advances in AA-2βG research,covering key aspects including discovery,structure,natural sources,extraction and detection methods,chemical and in vitro enzymatic synthesis,biosynthetic pathways,as well as applications in health care,skin care,and functional foods.Additionally,we highlight strategies for leveraging plant resources and enhancing AA-2βG biosynthesis,which are expected to accelerate future research and support the sustainable development and utilization of AA-2βG and other high-value natural products.
文摘Objectives:The current treatment options and therapeutic targets for triple-negative breast cancer(TNBC),an aggressive subtype of breast cancer(BrCA),are limited.This study aimed to identify novel biomarkers and transcriptional regulatory networks(TRN)inherent in TNBC samples.Methods:We analyzed pan-cancer BrCA datasets from The Cancer Genome Atlas(TCGA)to compare triple-positive breast cancer(TPBC)with TNBC.TRN algorithms and virtual inference of protein-enriched regulon(VIPER)were used to identify master regulators and their target genes.Utilizing TNBC cells(MDA-MB-231 and MDA-MB-468),we validated the relationship of nuclear factor erythroid 2-like 3(NFE2L3)and basic helix-loop-helix family member E 40(BHLHE40)by performing a luciferase assay.The expression levels of these targets were measured after transfections with plasmid and siRNA via qRT-PCR and western blots.The effect of these genes on cell proliferation and migration was studied using phenotypic assays.Results:Using computational approaches,we identified NFE2L3 as a master regulator with BHLHE40 as its target gene.NFE2L3 protein binds to the promoter region of BHLHE40 and regulates its transcriptional activity.Additionally,silencing and overexpressing NFE2L3 and BHLHE40 in TNBC cell lines MDA-MB-231 and MDA-MB-468 showed that NFE2L3 directly regulates BHLHE40 at both transcriptional and translational levels.We found that BHLHE40 requires NFE2L3 for cell proliferation and migration in TNBC.Conclusion:These findings underscore the significance of NFE2L3 and BHLHE40 in TNBC,highlighting NFE2L3’s role in regulating the oncogenic activity of BHLHE40 in TNBC cells.
