Objective:To observe the effect of respiratory-function changes on urinary output and expression of aquaporin (AQP) in kidney tissue in bronchial asthma (BA) model mice.To explore the correlation between the lung cont...Objective:To observe the effect of respiratory-function changes on urinary output and expression of aquaporin (AQP) in kidney tissue in bronchial asthma (BA) model mice.To explore the correlation between the lung controlling breathing and the lung regulating the waterways,and observe regulation by the lung-diffusing herb platycodon root (Platycodon grandiflorus (JACQ.) A.DC.).Methods:Forty-five healthy female Balb/c mice were divided randomly into normal,model and platycodon root groups.The BA model was replicated by complex sensitization and stimulation with ovalbumin (OVA).Changes in airway resistance were detected using an AniRes2005 system,and 24-hour urine output collected by metabolic cages.Histopathologic changes in the lung and kidney were observed by H&rE staining.Expression of the mRNA of AQP1 and AQP2 was detected by reverse transcription-polymerase chain reaction,immunohistochemistry,and immunofluorescence.Results:Compared with the normal group,airway resistance in the inspiratory phase intensified in the model group (P <.01).Following the pathologic changes in lung tissue,but no significant change in kidney tissue,24-hour urinary output decreased significantly (P <.05),and levels of AQP1,AQP2 and their mRNA increased significantly in the model group (P <.01).Compared with the model group,airway resistance in the inspiratory phase was weakened(P <.01).The urinary output increased (P <.05),pathologic changes in lung tissues decreased,and renal expression of AQP1,AQP2 and their mRNA decreased significantly (P <.01) in the platycodon root group.Conclusion:Changes in respiratory function in BA model mice can affect how the lung regulates water pathways.Platycodon root diffusing the lung can ameliorate the respiratoryfunction and pathologic changes in the lung tissues of BA model mice,but also regulate urinary output and renal expression of AQP1 and AQP2.展开更多
通过春季、夏季、秋季不同季节红椎(Castanopsis hystrix A. DC.)造林对比实验,对红椎造林成活率、幼树生长(高径)、树木形质生长和健康状况进行调查评价。试验结果表明:红椎在春季造林成活率最高,夏季造林次之。迹地全光造林夏季红椎...通过春季、夏季、秋季不同季节红椎(Castanopsis hystrix A. DC.)造林对比实验,对红椎造林成活率、幼树生长(高径)、树木形质生长和健康状况进行调查评价。试验结果表明:红椎在春季造林成活率最高,夏季造林次之。迹地全光造林夏季红椎平均胸径和平均高等调查因子比春季高。秋季造林生长状况最差;与米老排林冠下更新补植相比,迹地全光种植红椎生长较快较好。展开更多
基金This study was supported by the National Natural Science Foundation of China(81373503).
文摘Objective:To observe the effect of respiratory-function changes on urinary output and expression of aquaporin (AQP) in kidney tissue in bronchial asthma (BA) model mice.To explore the correlation between the lung controlling breathing and the lung regulating the waterways,and observe regulation by the lung-diffusing herb platycodon root (Platycodon grandiflorus (JACQ.) A.DC.).Methods:Forty-five healthy female Balb/c mice were divided randomly into normal,model and platycodon root groups.The BA model was replicated by complex sensitization and stimulation with ovalbumin (OVA).Changes in airway resistance were detected using an AniRes2005 system,and 24-hour urine output collected by metabolic cages.Histopathologic changes in the lung and kidney were observed by H&rE staining.Expression of the mRNA of AQP1 and AQP2 was detected by reverse transcription-polymerase chain reaction,immunohistochemistry,and immunofluorescence.Results:Compared with the normal group,airway resistance in the inspiratory phase intensified in the model group (P <.01).Following the pathologic changes in lung tissue,but no significant change in kidney tissue,24-hour urinary output decreased significantly (P <.05),and levels of AQP1,AQP2 and their mRNA increased significantly in the model group (P <.01).Compared with the model group,airway resistance in the inspiratory phase was weakened(P <.01).The urinary output increased (P <.05),pathologic changes in lung tissues decreased,and renal expression of AQP1,AQP2 and their mRNA decreased significantly (P <.01) in the platycodon root group.Conclusion:Changes in respiratory function in BA model mice can affect how the lung regulates water pathways.Platycodon root diffusing the lung can ameliorate the respiratoryfunction and pathologic changes in the lung tissues of BA model mice,but also regulate urinary output and renal expression of AQP1 and AQP2.
文摘通过春季、夏季、秋季不同季节红椎(Castanopsis hystrix A. DC.)造林对比实验,对红椎造林成活率、幼树生长(高径)、树木形质生长和健康状况进行调查评价。试验结果表明:红椎在春季造林成活率最高,夏季造林次之。迹地全光造林夏季红椎平均胸径和平均高等调查因子比春季高。秋季造林生长状况最差;与米老排林冠下更新补植相比,迹地全光种植红椎生长较快较好。
