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我国首次发现由Alternaria arborescens引起的甘薯叶柄和茎秆枯萎病
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作者 张成玲 唐伟 +7 位作者 马居奎 董谢楠 陈晶伟 高方园 杨冬静 梁昭 佟聪 孙厚俊 《植物保护》 北大核心 2026年第2期206-211,共6页
2021年-2023年从山东、云南等地采集在叶片、叶柄及茎秆等部位产生椭圆形病斑的甘薯样品,通过组织分离法获得病原菌,标为Z4hei和YN2,采用形态学和分子生物学技术对其鉴定。结果表明,在PDA培养基上2个菌株菌落初为白色后变为墨绿色至黑... 2021年-2023年从山东、云南等地采集在叶片、叶柄及茎秆等部位产生椭圆形病斑的甘薯样品,通过组织分离法获得病原菌,标为Z4hei和YN2,采用形态学和分子生物学技术对其鉴定。结果表明,在PDA培养基上2个菌株菌落初为白色后变为墨绿色至黑色。分生孢子椭圆形或豆荚状,具1~6个横隔,0~2个纵隔。2个菌株的rDNA-ITS序列长度均为569 bp,一致性为100%,与NCBI上交链格孢Alternaria alternata(MK370642)和枝状链格孢A.arborescens(PP097783)一致性最高,为100%。为进一步确定病原菌种类,分别扩增菌株的RPB2与TEF1-α基因,2个菌株RPB2长度均为985 bp,一致性为100%,与A.arborescens(XM_028644303)一致性最高,为99.59%。2个菌株的TEF1-α基因序列长度均为280 bp,一致性为99.60%,与NCBI上A.arborescens(OK081882)一致性最高,分别为99.29%和98.93%。由此证实引起我国甘薯叶柄和茎秆枯萎病的病原菌是A.arborescens,为该病在我国发生的首次报道,本研究为其防治提供了理论依据。 展开更多
关键词 甘薯 叶柄和茎秆枯萎病 枝状链格孢 病原菌鉴定
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NaCl胁迫下钙对沙拐枣(Calligonum arborescens)株高及光合特性的影响 被引量:6
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作者 王静静 张文鹏 徐当会 《中国沙漠》 CSCD 北大核心 2015年第1期167-174,共8页
研究了在NaCl胁迫下添加钙盐对沙拐枣(Calligonum arborescens)株高、净光合速率、PSII光化学效率、叶绿素含量等的影响,探讨了外源钙盐对植物在NaCl胁迫下的缓解作用。结果表明:随着NaCl浓度的升高,在未添加钙盐的情况下,植株的株高、... 研究了在NaCl胁迫下添加钙盐对沙拐枣(Calligonum arborescens)株高、净光合速率、PSII光化学效率、叶绿素含量等的影响,探讨了外源钙盐对植物在NaCl胁迫下的缓解作用。结果表明:随着NaCl浓度的升高,在未添加钙盐的情况下,植株的株高、净光合速率、Fv/Fm、叶绿素含量均呈下降趋势;8g·L-1 NaCl浓度时,添加CaCl2后植株的净光合速率、Fv/Fm、叶绿素含量均高于同浓度Ca(NO3)2的处理,而16g·L-1 NaCl浓度时,Ca(NO3)2对NaCl胁迫的缓解作用好于CaCl2;当钙浓度为5mM时CaCl2对应的植株高度高于Ca(NO3)2,当钙浓度为10、15、20mM时Ca(NO3)2对株高的作用好。相对于生理指标而言,沙拐枣形态指标对NaCl胁迫不敏感;钙对NaCl胁迫的缓解作用与NaCl浓度有关,即存在一个最佳的钠/钙比值,高过或低于这个比值,钙盐的缓解作用就会下降;CaCl2和Ca(NO3)2对NaCl胁迫缓解作用取决于NaCl的浓度,当植物处于中等程度NaCl胁迫时,CaCl2对生理胁迫的缓解作用比Ca(NO3)2好,若植物所受NaCl胁迫程度比较严重,则Ca(NO3)2的缓解作用好。 展开更多
关键词 NaCL胁迫 钙盐 沙拐枣(Calligonum arborescens) 株高 光合速率 PSII光化学效率 叶绿素含量
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刺桐(Erythrina arborescens Roxb.)维管分生组织原形成层的发育 被引量:2
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作者 兰利琼 逯多 +1 位作者 傅华龙 包少康 《四川大学学报(自然科学版)》 CAS CSCD 北大核心 2001年第1期95-99,共5页
刺桐的一年生枝条 ,在距顶端约 180 μm处 ,由顶端分生组织分化出剩余分生组织 ;在距顶端约2 40 μm处 ,剩余分生组织分散形成原形成层束 .原形成层细胞向外、向内分别衍化出原生木质部和原生韧皮部 .由于原形成层细胞横分裂频率的不同 ... 刺桐的一年生枝条 ,在距顶端约 180 μm处 ,由顶端分生组织分化出剩余分生组织 ;在距顶端约2 40 μm处 ,剩余分生组织分散形成原形成层束 .原形成层细胞向外、向内分别衍化出原生木质部和原生韧皮部 .由于原形成层细胞横分裂频率的不同 ,产生长、短两类细胞 ,短细胞与束间细胞一样 ,含有淀粉粒 .原形成层束间的基本分生组织可以分裂产生新的维管束 . 展开更多
关键词 刺桐 原形成层 发育 豆科 维管 分生组织
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刺桐(Erythrina arborescens Roxb.)原形成层向维管形成层转化的研究 被引量:2
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作者 兰利琼 包少康 +2 位作者 李平 卿人韦 傅华龙 《四川大学学报(自然科学版)》 CAS CSCD 北大核心 2002年第3期528-533,共6页
作者对刺桐原形成层向维管形成层转化的研究表明 ,刺桐的维管形成层为叠生形成层 ,由分散的、排列成环的原形成层束转化而来 ,其转化过程是渐进的 .在初生生长的很早阶段就出现了长、短两类细胞 ,即原初的纺锤状原始细胞和原初的射线原... 作者对刺桐原形成层向维管形成层转化的研究表明 ,刺桐的维管形成层为叠生形成层 ,由分散的、排列成环的原形成层束转化而来 ,其转化过程是渐进的 .在初生生长的很早阶段就出现了长、短两类细胞 ,即原初的纺锤状原始细胞和原初的射线原始细胞 .它们分别发育转化为维管形成层中的纺锤状原始细胞和射线原始细胞 .在转化过程中 ,长细胞的端壁由平截转变为尖削 ,短细胞则由短的长方形转变成近等径的多边形 .原初的射线原始细胞和射线原始细胞皆含有淀粉粒 ,纺锤状原始细胞的叠生状态直到次生生长出现以后才由于连续的径向垂周分裂而获得 .在 1~ 4年生枝中 ,随着年龄的增长 ,射线高度减小 ,宽度增加 .射线原始细胞可以伸长而转变成纺锤状原始细胞 ,从而将轴向的高射线隔分成矮射线 ,纺锤状原始细胞也可以由于横分裂而产生新的射线 .纺锤状原始细胞的长度一般为 170~ 2 90 μm ;射线原始细胞的长度一般为 2 8~ 5 3μm . 展开更多
关键词 刺桐 原形成层 维管形成层 转化 发育
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Effects of deficit irrigation on daily and seasonal variations of trunk sap flow and its growth in Calligonum arborescens 被引量:6
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作者 LiShan SHAN Yi LI +1 位作者 RuiFeng ZHAO XiMing ZHANG 《Journal of Arid Land》 SCIE CSCD 2013年第2期233-243,共11页
Water deficit in arid and semiarid regions affects whole-plant sap flow and leaf-level water relations. The objectives of this study were to clarify how sap flow of Calligonum arborescens responds to different drought... Water deficit in arid and semiarid regions affects whole-plant sap flow and leaf-level water relations. The objectives of this study were to clarify how sap flow of Calligonum arborescens responds to different drought stress conditions and to understand its acclimation mechanism to drought environments. A field experiment was conducted for C. arborescens during the growing season to evaluate the effects of deficit irrigation on the daily and seasonal variations of trunk sap flow in the shelterbelt along the Tarim Desert Highway, Xinjiang, China. Three dif- ferent water regimes (2,380, 1,960 and 1,225 m3/hm2) were applied at different stages of plant growth. From 1 May to 30 October 2007, a heat-balance stem flow gauge was used to monitor the sap flow dynamics of C. arborescens under different water regimes. Atmospheric evaporation demand and soil moisture conditions for differentially irri- gated C. arborescens were also monitored. The result showed that sap flow exhibited a clear diurnal pattern re- gardless of treatments; the diurnal patterns of sap flow and vapour pressure deficit were very similar under different water regimes and growing seasons, while the slope of the linear regression of this correlation confirmed an in- creasing water regime. The sap flow decreased under reduced water regimes and there was nocturnal sap flow regardless of water regimes, which was mainly contributed to nocturnal transpiration and water recharge. The sap flow peaked before midnight and dropped afterwards with obviously higher values in summer than in other seasons. It is speculated that the water consumption of C. arborescens during the day can be supplemented through the sap flow at night, which increased with increasing irrigation amount. Net radiation was the most significant correlated factor that influenced sap flow velocity and transpiration under different water regimes (R2〉0.719). Compared with the commonly practiced water regime, the growth of C. arborescens was significantly slower in the stress deficit irrigation, but not significantly different from that in the moderate deficit irrigation. The moderate deficit irrigation would not affect the stability of the shelterbelt and was a more efficient use of water resources compared with the current watering amount. 展开更多
关键词 Calligonum arborescens sap flow environmental factor growth parameter Tarim Desert Highway
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Genetic Variation in Morphophysiological Characters of Seeds of Ceratoides arborescens,a Species Endemic to China
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作者 王普昶 赵丽丽 +3 位作者 易津 张锦华 张莉 陈莹 《Agricultural Science & Technology》 CAS 2011年第5期695-698,750,共5页
[Objective] The research aimed at investigating the variation in morpho-physiological characteristics of Ceratoides arborescens seeds from Inner Mongolia grassland.[Method] The seeds of six wild C.arborescens populati... [Objective] The research aimed at investigating the variation in morpho-physiological characteristics of Ceratoides arborescens seeds from Inner Mongolia grassland.[Method] The seeds of six wild C.arborescens populations were collected from Siwangziqi,Xilinhaote,Keshiketengqi and Wengniuteqi in Inner Mongolia grassland;then a total of eight indicators of seed morphology and germination traits were determined,respectively.[Result] There was wide variation in seed traits among different populations of C.arborescens.The variation within the populations was higher than that among the populations,which meant that C.arborescens had a strong adaptability to environment pressure.The seed traits shared a significant positive correlation with each other(r0.931,P0.01).The three germination-related indices including seed germination rate,germination index,vitality index had significant positive correlations with each other(r 0.836,P0.01)and with seed setting rate(r0.896,P0.01),but shared weak correlations with morphological traits.There were significant correlations between pappus length and longitude,rainfall,as well as between seed width and temperature,rainfall(r0.801,P0.01).[Conclusion] The research will provide reference for the seed breeding and collection of germplasm resource.We suggest that collecting seeds from various individuals from each populations will be the preferred strategy to initiate plant improvement program of the species and for gene conservation in seed banks. 展开更多
关键词 Ceratoides arborescen Seed variation Seed germination
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凤丹皮内生真菌Alternaria arborescens发酵产物中甾醇类化合物 被引量:1
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作者 翟晓东 孙磊 +1 位作者 吴德玲 许凤清 《中南药学》 CAS 2023年第5期1203-1207,共5页
目的研究从铜陵凤凰山牡丹根皮中分离得到的一株内生菌Alternaria arborescens的次生代谢产物。方法应用硅胶柱色谱结合半制备高效液相色谱分离技术对发酵液的乙酸乙酯提取部位进行分离纯化,并经核磁共振、质谱分析,与文献对比鉴定结构... 目的研究从铜陵凤凰山牡丹根皮中分离得到的一株内生菌Alternaria arborescens的次生代谢产物。方法应用硅胶柱色谱结合半制备高效液相色谱分离技术对发酵液的乙酸乙酯提取部位进行分离纯化,并经核磁共振、质谱分析,与文献对比鉴定结构。结果分离得到6个甾醇类化合物,分别鉴定为(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(1)、麦角甾醇(2)、5α,8α-环氧-(22E,24R)麦角甾-6,22-二烯-3β-醇(3),5α,8α-环氧-(22E,24R)麦角甾-6,9,22-三烯-3β-醇(4),麦角甾-7,22-二烯-3β-醇(5)和β-谷甾醇(6)。结论化合物1~6为首次从该内生菌的发酵液中分离得到。 展开更多
关键词 alternaria arborescens 甾醇 牡丹皮 内生菌
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New flavonoids from bioactive extract of Algerian medicinal plant Launeae arborescens
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作者 Khaled Sekkoum Nasser Belboukhari Abdelkrim Cheriti 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2014年第4期267-271,共5页
Objective:To investigate the butanol fraction of the water/acetone extract and isolate of the new flavonoids from Launeae arboescens.Methods:The compounds were isolated by liquid chromatographic methods and their stru... Objective:To investigate the butanol fraction of the water/acetone extract and isolate of the new flavonoids from Launeae arboescens.Methods:The compounds were isolated by liquid chromatographic methods and their structures were identified by using spectroscopic analysis.Results:The isolated compounds were identified as:7-0-[α-rhamnopyranosyl 4',5,6-Trihydroxy flavone 1,4',5'-Di-Methoxy 7-(5"-Me Hexan)1-oyl flavanone 2,3"-isopropyl pyrano[1":7,4":6]3',4',5',5-Tetrahydroxy flavanone 3,5,4',5'-Tri-Hydroxy 7-(3"-Me butan)-yl flavanone 4.5,7-Dihydroxy-2',4',5'-trimethoxy-isoflavanone 5,5,6,7,4'-tetrahydroxy flavonol 6,7-O-[α-rhamnopyranosyl-(1->6)-β-glucopyranosyl]-4',5,7-tri-hydroxy-flavanone 7,7-O-[α-rhamnopyranosyl-(1->6)-β-glucopyranosyl]3',5-Dihydroxy 4'-Methoxy flavanone 8.Conclusions:The presence of different types of bioactive flavonoids in Launeae arhoescens extract can explain the large ethnopharmacological uses and the potential activity of this medicinal plant. 展开更多
关键词 Launeae arborescens asteraceae FLaVaNONE Isoflavanone Glycosid FLaVaNONE
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Erythrinarbine,a Novel nor-A ring Erythrina Alkaloid from Erythrina arborescens
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作者 Dong Lei YU Jian GUO +1 位作者 Li Zhen XU Shi Lin YANG(Institute of Medicinal Plant Development, Chinese Academy of Medical Sciencesand Peking Union Medical College, Beijing 100094) 《Chinese Chemical Letters》 SCIE CAS CSCD 1999年第2期139-142,共4页
Erythrinarbine 2, a novel alkaloid was isolated from the stem of Erythrina arborescens Roxb. Its structure and stereochemistry were elucidated on the basis of the spectral analysis (C-13 - H-1 COSY, H-1-H-1 COSY, NOES... Erythrinarbine 2, a novel alkaloid was isolated from the stem of Erythrina arborescens Roxb. Its structure and stereochemistry were elucidated on the basis of the spectral analysis (C-13 - H-1 COSY, H-1-H-1 COSY, NOESY and HMBC). 展开更多
关键词 Erythrina arborescens erythrinarbine nor-a ring erythrina alkaloid
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Vascular bundle connection between seed stalk and seed coat of Caragana arborescens
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作者 Zhihui Luan Daowei Zhou Diankun Shao 《Journal of Forestry Research》 SCIE CAS CSCD 2018年第1期253-257,共5页
The purpose of this study was to investigate the morphology, transmission route of photosynthetic products in the seed stalk of Caragana arborescens Lam. at various stages after flowering and evaluate the role of the ... The purpose of this study was to investigate the morphology, transmission route of photosynthetic products in the seed stalk of Caragana arborescens Lam. at various stages after flowering and evaluate the role of the seed stalk in seed maturation using scanning electron microscopy. We found that the mature seed stalk mainly consisted of vessels,sclerenchymatous cells and parenchymatous cells. Elongated cells formed a ring at the junction between the seed stalk and seed, and a large hollow was produced by two layers of sclerenchymatous cells in the interior seed, which functions to sustain seed growth. The opening in the center of the ring was closed during an early stage of seed development, and the opening enlarged as the seed matured.There were two routes for material transport, which started from the seed stalk: one from the seed coat to the embryo,and another directly entering the embryo. Vessels, which are abundant in the seed stalk, are the pathway for absorption of nutrients by the seeds. The vessels were densely packed in the seed stalk, and secondary thickening of the cell wall of the vessels was characterized by net thread thickening, while the secondary thickening of the cell wall of the vessels within the seed was characterized by screw thread thickening. The morphological characteristics of the vessels were adaptive to its functions. 展开更多
关键词 Caragana arborescens Seed coat Seed stalk Vascular bundle connection
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New isoflavonoids from Erythrina arborescens and structure revision of anagyroidisoflavone A
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作者 Fei WANG Xu-Long LI +2 位作者 Guo-Zhu WEI Fu-Cai REN Ji-Kai LIU 《Natural Products and Bioprospecting》 CAS 2013年第5期238-242,共5页
Five hitherto unknown isoflavonoids,namely erythrinins D-H(1-5),were isolated from the ethanol extract of Erythrina arborescens.Their structures were elucidated on the basis of extensive spectroscopic studies.In addit... Five hitherto unknown isoflavonoids,namely erythrinins D-H(1-5),were isolated from the ethanol extract of Erythrina arborescens.Their structures were elucidated on the basis of extensive spectroscopic studies.In addition,the structure of anagyroidisoflavone A(6a)has been revised as 1″-O-methylerythrinin F(6)by re-analysis of the original spectroscopic data. 展开更多
关键词 Erythrina arborescens ISOFLaVONOID erythrinin structure revision
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Artificial Erythrina Alkaloids from Three Erythrina Plants,E. variegata,E. crista-galli and E. arborescens
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作者 Bing-Jie Zhang Jing Wu +2 位作者 Mei-Fen Bao Fang Wang Xiang-Hai Cai 《Natural Products and Bioprospecting》 CAS 2020年第2期57-66,共10页
Fourteen unprecedented artificial Erythrina alkaloids were isolated from the Erythrina variegata,E.crista-galli and E.arborescens(Fabaceae).The structures of these alkaloids were determined by spectroscopic analyses.T... Fourteen unprecedented artificial Erythrina alkaloids were isolated from the Erythrina variegata,E.crista-galli and E.arborescens(Fabaceae).The structures of these alkaloids were determined by spectroscopic analyses.Their possible forma-tions were proposed.All isolated compounds showed no cytotoxicity and hypoglycemic activity at cell screening bioassay. 展开更多
关键词 FaBaCEaE Erythrina variegata E.crista-galli E.arborescens artificial products Erythrina alkaloids
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Evaluation of the Anticonvulsant Activity of the Leaf Methanol Extract of Crassula arborescens (Mill.) Willd. (Crassulaceae) in Mice
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作者 George Jimboyeka Amabeoku Oluchi Nneka Mbamalu Tasneem Davids Samukelisiwe Fakude Anda Gqwaka Fiona Mbai Reighman Pieterse Aneesa Shaik 《Journal of Pharmacy and Pharmacology》 2014年第7期393-403,共11页
Crassula arborescens (Mill.) Willd. subsp. Arborescens is widely used for the treatment of various ailments including diarrhoea, corns, epilepsy and as a purgative. However, no information exists in any literature t... Crassula arborescens (Mill.) Willd. subsp. Arborescens is widely used for the treatment of various ailments including diarrhoea, corns, epilepsy and as a purgative. However, no information exists in any literature to verify the acclaimed effectiveness of C. arborescens in the treatment of the various ailments. The study, therefore, intended to investigate the anticonvulsant activity of the leaf methanol extract of C. arborescens in mice. Acute toxicity study and phytochemical qualitative analysis of the plant extracts were also carried out. Chemically-induced convulsion methods were used to assess the anticonvulsant activity of C. arborescens. Standard methods were used for the acute toxicity study and phytochemical analysis of the chemical components of the plant extract. PTZ (pentylenetetrazole), bicuculline, picrotoxin, NMDLA (N-methyl-DL-aspartic acid) or strychnine produced tonic convulsions in all the mice used. Leaf methanol extract of Crassula arborescens, muscimol, phenobarbitone or diazepam significantly antagonised PTZ, bicuculline or picrotoxin-induced convulsion. C. arborescens or LY233053 significantly antagonised NMDLA-induced tonic convulsion. C. arborescens or phenobarbitone significantly antagonised strychnine-elicited tonic convulsion. Phenytoin or DMSO (dimethylsulfoxide) did not significantly affect the tonic convulsion produced by PTZ, bicuculline, picrotoxin, NMDLA or strychnine. The LDso value obtained from intraperitoneal administration of C. arborescens was 781.6 mg/kg while that following oral administration of the plant extract was over 4,000 mg/kg. The phytochemical qualitative analysis done showed the presence of flavonoids, tannins, reducing sugar, saponins and triterpene steroids. The data obtained in the study show that the leaf methanol extract of Crassula arborescens has anticonvulsant activity which may be underpinned by GABAergic, glutaminergic and glycinergic mechanisms. The high LDso value obtained following the oral administration of the plant extract shows that the leaf methanol extract is non-toxic to animals. 展开更多
关键词 Crassula arborescens CRaSSULaCEaE anticonvulsant activity GaBaERGIC glutamatergic and glycinergic mechanisms mice.
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NaCl胁迫下苞片对华北驼绒藜种子萌发的影响 被引量:9
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作者 薛建国 韩建国 +3 位作者 柳小妮 邓波 王显国 张铁军 《草业学报》 CSCD 2008年第6期60-65,共6页
对去苞片和带苞片的华北驼绒藜种子在不同的盐浓度下进行处理,带苞片种子的发芽率、发芽指数、活力指数均高于去苞片的。在NaCl浓度为70,140,210,280和420 mmol/L时,带苞片种子的发芽率显著高于去苞片的(P<0.05),分别为90.00%,89.00%... 对去苞片和带苞片的华北驼绒藜种子在不同的盐浓度下进行处理,带苞片种子的发芽率、发芽指数、活力指数均高于去苞片的。在NaCl浓度为70,140,210,280和420 mmol/L时,带苞片种子的发芽率显著高于去苞片的(P<0.05),分别为90.00%,89.00%,79.75%,69.25%,53.75%和78.50%,81.50%,74.00%,62.25%和39.25%。用蒸馏水和490 mmol/L的NaCl溶液对带苞片和去苞片的华北驼绒藜的种子处理12 h,带苞片种子吸水率均低于去苞片的,苞片延缓了种子的吸水率。490 mmol/L NaCl处理华北驼绒藜种子1,3和7 d后,苞片中Na+含量显著增加(P<0.05)。说明华北驼绒藜种子的苞片可以吸附一部分Na+,减少对胚的伤害。苞片对华北驼绒藜在荒漠盐渍环境中的定植具有重要的意义。 展开更多
关键词 华北驼绒藜 NaCL胁迫 苞片 种子曲发
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NaCl和PEG对华北驼绒藜和梭梭种子萌发的影响 被引量:20
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作者 薛建国 韩建国 +3 位作者 王显国 邓波 张铁军 柳小妮 《草地学报》 CAS CSCD 2008年第5期470-474,共5页
采用不同浓度NaCl和等渗PEG 6000处理华北驼绒藜(Ceratoides arborescens(Losinsk.)Tsien et C.C.Ma)和梭梭(Haloxylon ammodendron(Mey.)Bunge)2种旱生植物种子。结果表明,盐胁迫和水分胁迫对其萌发均具有明显的抑制作用,降低种子萌发... 采用不同浓度NaCl和等渗PEG 6000处理华北驼绒藜(Ceratoides arborescens(Losinsk.)Tsien et C.C.Ma)和梭梭(Haloxylon ammodendron(Mey.)Bunge)2种旱生植物种子。结果表明,盐胁迫和水分胁迫对其萌发均具有明显的抑制作用,降低种子萌发率,推迟种子初始萌发时间、延长种子萌发时间;在700 mmol/L NaCl处理时,华北驼绒藜和梭梭种子萌发率分别为0和49.33%,说明梭梭比华北驼绒藜种子具有更强的耐盐性。PEG6000(-3.2MPa)溶液处理后,2种旱生植物种子萌发恢复率高于等渗NaCl溶液处理;在NaCl(-3.2MPa)溶液中处理10 h后,华北驼绒藜和梭梭种子中Na+含量增加,K+含量减少,表明种子中Na+的大量累积,能够抑制种子的萌发。 展开更多
关键词 NaCL PEG 华北驼绒藜 梭梭 萌发率
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FAAS法测定芦荟中的微量元素 被引量:1
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作者 甄攀 王爽 +3 位作者 王煜 董慧 王清云 成蔚 《河北北方学院学报(自然科学版)》 2011年第2期27-30,共4页
目的利用火焰原子吸收光谱法(FAAS)测定不同品种芦荟中微量元素的含量.方法以浓硝酸:高氯酸(体积比4∶1)为消化液,在常压微沸条件下对芦荟样品进行消化.采用空气-乙炔火焰原子吸收光谱法测定几种芦荟中微量元素含量.结果测定了五种芦荟... 目的利用火焰原子吸收光谱法(FAAS)测定不同品种芦荟中微量元素的含量.方法以浓硝酸:高氯酸(体积比4∶1)为消化液,在常压微沸条件下对芦荟样品进行消化.采用空气-乙炔火焰原子吸收光谱法测定几种芦荟中微量元素含量.结果测定了五种芦荟样品中的Ca、Mg、Zn、Fe、Cu、Mn、Cd等7种微量元素,这几种芦荟中均含有丰富的Ca、Mg元素,Cd元素均未检出.木立芦荟和不夜城芦荟中这几种微量元素的含量较高.除Ca、Mg元素外,华芦荟中Zn的含量最高;库拉索芦荟和海虎兰中Fe的含量最高;木立芦荟中Fe、Zn的含量都较高,而且凝胶中的含量高于其叶皮中的含量;不夜城芦荟中Fe、Zn的含量都较高,但其叶皮中的含量高于凝胶中的含量.结论不同品种的芦荟中微量元素含量不同;芦荟叶皮与其凝胶汁液中微量元素含量不同. 展开更多
关键词 华芦荟 库拉索芦荟 木立芦荟 不夜城芦荟 海虎兰 微量元素 火焰原子吸收光谱法
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基于ITS2序列的海桐皮及其混伪品DNA分子鉴定 被引量:10
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作者 田荣 吴云 +4 位作者 谷巍 单鸣秋 谷宇琛 马丽杰 裴凌峰 《中草药》 CAS CSCD 北大核心 2021年第1期211-218,共8页
目的应用ITS2条形码技术,对海桐皮ErythrinaeCortex及其混伪品进行分子鉴定。方法通过提取31份海桐皮药材基原植物刺桐E.variegata、乔木刺桐E.arborescens及其混伪品的基因组DNA,扩增ITS2序列并测序;同时从GenBank下载混伪品序列15种38... 目的应用ITS2条形码技术,对海桐皮ErythrinaeCortex及其混伪品进行分子鉴定。方法通过提取31份海桐皮药材基原植物刺桐E.variegata、乔木刺桐E.arborescens及其混伪品的基因组DNA,扩增ITS2序列并测序;同时从GenBank下载混伪品序列15种38条,运用MEGA7.0软件进行序列比对,进行种间序列差异分析比较,并构建Neighbor-jioning(NJ)系统进化树,预测ITS2二级结构。结果在海桐皮2种基原中,刺桐的ITS2序列长度为232bp,乔木刺桐的ITS2序列长度为230bp,均为单倍型,可以明显区分;同时与其他刺桐属及易混品之间遗传距离较远。NJ树结果显示刺桐、乔木刺桐及其他易混品均单独聚为一支,表现出良好的单系性;依据ITS2二级结构,刺桐、乔木刺桐及其混伪品在4个螺旋区的茎环数目、大小、位置以及螺旋发出时的角度均有明显差异,可以直观地将刺桐与乔木刺桐,以及其与易混品进行区分。结论ITS2序列作为DNA条形码能稳定、准确鉴别海桐皮药材,为保障安全用药提供了新的技术手段。 展开更多
关键词 海桐皮 刺桐 乔木刺桐 易混品 ITS2 物种鉴定
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木立芦荟中BACE抑制活性成分的研究 被引量:2
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作者 高博 姚春所 +2 位作者 周金云 陈若芸 方唯硕 《药学学报》 CAS CSCD 北大核心 2006年第10期1000-1003,共4页
目的从木立芦荟(Aloe arborescens)中寻找新的BACE(β-分泌酶)抑制活性成分。方法用硅胶正相色谱及反相色谱技术进行活性成分的分离纯化,根据理化性质、光谱资料进行结构鉴定。结果从木立芦荟95%乙醇提取物的乙酸乙酯部分和正丁醇部分,... 目的从木立芦荟(Aloe arborescens)中寻找新的BACE(β-分泌酶)抑制活性成分。方法用硅胶正相色谱及反相色谱技术进行活性成分的分离纯化,根据理化性质、光谱资料进行结构鉴定。结果从木立芦荟95%乙醇提取物的乙酸乙酯部分和正丁醇部分,分离得到了8个化合物:异丁酰基芦荟宁A(1),芦荟宁A(2),芦荟大黄素(3),(E)-2-acetonyl-8-(2′-O-feru loxyl)-β-D-glucopyranosyl-7-m ethoxy-5-m ethyl-chromone(4),7-O-m ethyl aloeresin A(5),芦荟苷A(6),elgon ica-d im er A(7)和elgon ica-d im er B(8)。结论化合物1为新化合物,化合物4为首次从该植物中分离得到。活性测试结果表明,化合物2,4,5,6对BACE有一定的抑制活性。 展开更多
关键词 木立芦荟 BaCE抑制活性 芦荟宁衍生物
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Synovial lipomatosis: A rare cause of knee pain in an adolescent female 被引量:1
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作者 Nicholas Miladore Mary A Childs Vani J Sabesan 《World Journal of Orthopedics》 2015年第3期369-373,共5页
Synovial lipomatosis is a benign proliferative disease of the subsynovial adipose which can lead to a variety of presentations. Cases of synovial lipomatosis in children or adolescents are rare. This case report descr... Synovial lipomatosis is a benign proliferative disease of the subsynovial adipose which can lead to a variety of presentations. Cases of synovial lipomatosis in children or adolescents are rare. This case report describes an adolescent patient with a rare bilateral presentation of synovial lipomatosis. She had been treated for years prior to her presentation for juvenile idiopathic arthritis. She presented with chronic bilateral knee pain, swelling, and mechanical symptoms. Bilateral MR imaging demonstrated effusions, hypertrophy of the synovium, and polyp-like projections of tissue with the same signal intensity as fat which is pathognomonic for synovial lipomatosis. Arthroscopic synovectomy and extensive debridement of polyp like fat projections of the right knee was performed. Histopathology was consistent with the synovial lipomatosis diagnosis. Postoperatively, the patient was satisfied with her outcome with improved pain relief and function in her right knee. 展开更多
关键词 SYNOVIaL LIPOMaTOSIS LIPOMaTOSIS KNEE aRTHROSCOPY LIPOMa arborescens aRTHROSCOPY VILLOUS lipomatous proliferation of the SYNOVIaL membrane KNEE pain
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PEG与NaCl等渗胁迫对华北驼绒藜种子萌发及幼苗生长影响的差异 被引量:9
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作者 高嵩 王俊锋 敖云娜 《中国草地学报》 CSCD 北大核心 2021年第5期115-120,共6页
设置不同等渗梯度(0MPa、-0.4MPa、-0.6MPa、-0.8MPa、-1.0MPa)模拟研究生理干旱(采用NaCl模拟)与土壤干旱(采用PEG模拟)对华北驼绒藜种子萌发和幼苗生长的影响。结果表明:与对照(CK,0MPa)相比,轻度NaCl胁迫(-0.4MPa)对种子发芽率、发... 设置不同等渗梯度(0MPa、-0.4MPa、-0.6MPa、-0.8MPa、-1.0MPa)模拟研究生理干旱(采用NaCl模拟)与土壤干旱(采用PEG模拟)对华北驼绒藜种子萌发和幼苗生长的影响。结果表明:与对照(CK,0MPa)相比,轻度NaCl胁迫(-0.4MPa)对种子发芽率、发芽指数和幼苗活力指数无显著影响,中度胁迫(-0.6MPa)使三者均大幅增加,重度胁迫(-0.8~-1.0MPa)使种子平均发芽率、发芽指数显著降低但对幼苗活力指数无明显影响;轻度和中度PEG胁迫(-0.4~-0.6MPa)对发芽率、发芽指数无显著影响,但显著提高了幼苗活力指数,重度PEG胁迫(-1.0MPa)使发芽率和发芽指数均显著降低。对幼苗而言,NaCl胁迫对其无明显影响,但轻度、中度、重度PEG胁迫使根长度均显著增加。总而言之,两种胁迫对种子萌发的影响极为不同,轻度、中度生理干旱促进种子萌发,重度生理干旱抑制种子萌发,而土壤干旱胁迫对萌发无明显影响;两种胁迫对幼苗生长的影响主要与二者能否促进根系生长有关,生理干旱不影响根系生长,土壤干旱促进根生长。 展开更多
关键词 华北驼绒藜 种子萌发 幼苗 NaCL胁迫 PEG胁迫
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