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A Learning-Driven Visual Servoing Framework for Latency Compensation in Image-Guided Teleoperation
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作者 Junmin Lyu Feng Bao +4 位作者 Guangyu Xu Siyu Lu Bo Yang Yuxin Liu Wenfeng Zheng 《Computer Modeling in Engineering & Sciences》 2026年第2期831-847,共17页
Robust teleoperation in image-guided interventions faces critical challenges from latency,deformation,and the quasi-periodic nature of physiological motion.This paper presents a fully integrated,latency-aware visual s... Robust teleoperation in image-guided interventions faces critical challenges from latency,deformation,and the quasi-periodic nature of physiological motion.This paper presents a fully integrated,latency-aware visual servoing system leveraging stereo vision,hand–eye calibration,and learning-based prediction for motion-compensated teleoperation.The system combines a calibrated binocular camera setup,dual robotic arms,and a predictive control loop incorporating Long Short-Term Memory(LSTM)and Temporal Convolutional Network(TCN)models.Through experiments using both in vivo and phantom datasets,we quantitatively assess the prediction accuracy and motion-compensation performance of both models.Results show that TCNs deliver more stable and precise tracking,especially on regular trajectories,while LSTMs exhibit robustness under quasi-periodic dynamics.By matching prediction horizons to system latency,the approach significantly reduces peak and steady-state tracking errors,demonstrating practical feasibility for deploying prediction-augmented servoing in teleoperated surgical. 展开更多
关键词 Teleoperation system motion prediction surgical robot visual servoing learning-based control
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Offset fabrication of off-axis aspherical surfaces with large off-axis amounts by slow tool servo single-point diamond turning
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作者 Menghui Lan Bing Li +1 位作者 Xiang Wei Xiuyuan Wu 《Chinese Journal of Mechanical Engineering》 2026年第1期179-190,共12页
Off-axis aspherical mirrors are widely used in optical systems and precision measuring instruments,whereas off-axis aspherical mirrors with large sizes and off-axis are used in large optical systems such as astronomic... Off-axis aspherical mirrors are widely used in optical systems and precision measuring instruments,whereas off-axis aspherical mirrors with large sizes and off-axis are used in large optical systems such as astronomical telescopes and radio telescopes.However,if the off-axis amount of an off-axis aspherical mirror exceeds the capability of the machine tool,traditional rotary-turning machining methods are not applicable,and advanced computerized numerical control(CNC)machining methods,such as the slow-tool-servo method,must be im-plemented.This article proposes a non-conventional offset(NCO)fabrication method based on slow-tool-servo single-point diamond turning for machining off-axis aspherical surfaces with large off-axis amounts.This method is theoretically applicable to the machining of off-axis aspherical surfaces with any off-axis amount.NCO fab-rication is a simpler and more efficient path-planning solution for machining individual off-axis parabolic sur-faces.In addition,corresponding solutions for other types of aspherical surfaces are proposed using the NCO method.The turning depths of workpieces with different off-axis amounts at the same machining position are analyzed and compared.A specific measurement scheme for the NCO method is presented,and the experimental results indicate that the PV and RMS form errors are 0.658μm and 60 nm,respectively.This work demonstrates that the NCO method can effectively deal with the machining challenges of off-axis aspherical structures with large off-axis amounts. 展开更多
关键词 Offset fabrication Off-axis aspheric Slow tool servo Single-point diamond turning
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Highly enhanced scandium extraction and back-extraction efficiencies using a new C272–iso-octanol synergistic system
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作者 Ziyun Zhang Yanlin Zhang +5 位作者 Wenyu Shen Dapeng Guo Hongbo Wang Duo Wang Fang Zhou Chao Yang 《Chinese Journal of Chemical Engineering》 2026年第1期198-207,共10页
This article presents a new synergistic extraction system composed of Cyanex 272(C272,bis(2,4,4-trimethylpentyl)phosphinic acid)and iso-octanol for Sc_(3+) separation.The proposed synergistic system possessed an Sc^(3... This article presents a new synergistic extraction system composed of Cyanex 272(C272,bis(2,4,4-trimethylpentyl)phosphinic acid)and iso-octanol for Sc_(3+) separation.The proposed synergistic system possessed an Sc^(3+) extraction efficiency of 93.5%and a back-extraction efficiency of 82.7%,with selectivity coefficients of β_(Sc/Fe)=459 and β_(Sc/Al)=4241,which are considerably higher as compared to the current extraction systems.The extraction mechanism was studied and interpreted.The enhanced extraction efficiency is attributed to the increased hydrophobicity of the ternary complex,whereas the back-extraction efficiency can be ascribed to the attenuated stability of the complex.C272 and C272–iso-octanol systems also possess considerable surface activity,which is beneficial for the phase separation in solvent extraction.Based on the solvent extraction results,a preliminary study was conducted on polymer inclusion membranes(PIMs)using the binary system for Sc^(3+) separation to avoid the formation of the third phase,achieving an optimal initial flux of PIM of 6.71×10^(−4)mol·m^(−2)·h^(−1).Our results provide valuable information on highly efficient Sc^(3+) separation,and the study on PIM extraction has shown a green alternative to solvent extraction. 展开更多
关键词 ScaNDIUM Solvent extraction Polymer inclusion membrane c272 Iso-octanol
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The centennial legacy of disturbances on sedimentation and carbon burial in an estuarine mangrove ecosystem,South China
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作者 Juan SU Yaobei LIN +5 位作者 Penghui LI Zhangcai QIN Luhua XIE Fan WANG Weiguang LI Jinghong ZHANG 《Journal of Oceanology and Limnology》 2026年第1期109-124,共16页
Both natural and human-induced disturbances affect the normal functioning and services of mangrove ecosystems.To address the consequences of intense human and climatic disturbances on sedimentation and carbon burial,s... Both natural and human-induced disturbances affect the normal functioning and services of mangrove ecosystems.To address the consequences of intense human and climatic disturbances on sedimentation and carbon burial,sediment cores from the last remaining mangrove Kandelia obovata forest and an adjacent mudflat in the densely populated and typhoon-prone Zhujiang(Pearl)River estuary of China,were analyzed using methods including^(210)Pb dating andδ^(13)C analysis.Results indicate that after damming in the 1950s,during 1960-1980,the natural establishment of K.obovata forests initiated the insitu sedimentation.As these forests matured during 1980-1990,they significantly boosted siltation in the region on mudflat.During 1990-2015,the invasion of Spartina alterniflora and land reclamation for aquaculture caused infiltration of coarse sediments and the impacts of typhoons were recorded within the K.obovata forest,while no clear typhoon record was observed on the mudflat.Since 2015,reforestation efforts with S.apetala that began in 1999 have reversed the effects of earlier deforestation.Over time,mangroves established a rapid autochthonous carbon burial that grew as the forests age,potentially surpassing the influx of allochthonous carbon due to deforestation.The reforestation also immediately improved carbon burial on the mudflat,which stabilized after a decade due to the rapid growth and high biomass of S.apetala.Overall,the K.obovata forest demonstrated a stronger sedimentation and carbon burial capabilities than the mudflat,with a surplus of 35.2 Mg C/hm^(2)in soil organic carbon stock and 1.0 Mg C/(hm^(2)·a)in burial rate.Organic matter dissolved in soil was mainly humus-like components,and mangrove inputs likely increased the degree of humification.This study offered direct evidence regarding the impact of multiple disturbances on local and regional sedimentation and carbon burial,and future management strategies. 展开更多
关键词 MaNGROVE DEFORESTaTION REFORESTaTION allochthonous source 210Pb dating δ^(13)c
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Smooth switching mechanism-based adaptive integral terminal SMC for PMSM servo system with stator voltage saturation and unknown disturbances 被引量:1
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作者 Xiangxiang Meng Haisheng Yu +1 位作者 Jie Zhang Qing Yang 《Control Theory and Technology》 2025年第2期294-309,共16页
This article investigates the anti-disturbance and stabilization problems for the nonlinear uncertain permanent magnet synchronous motor(PMSM)with stator voltage saturation and unknown load.A smooth switching mechanis... This article investigates the anti-disturbance and stabilization problems for the nonlinear uncertain permanent magnet synchronous motor(PMSM)with stator voltage saturation and unknown load.A smooth switching mechanism is presented to structure the adaptive integral terminal sliding mode control(SMC)strategy.The control design consists of compensation control and nominal control,which improves the rapidity and accuracy of trajectory tracking.The smooth saturation model based on the error function is applied to approximate the voltage saturation phenomenon.Additionally,to deal with the adverse effects of various unknown disturbances,including model parameter uncertainties and unknown external load disturbances,an improved disturbance observer(DO)is proposed.This observer effectively suppresses the fluctuations caused by fixed gain during the starting period of the system.Finally,the experimental results under different conditions show that the proposed strategy has good tracking and disturbance suppression performances. 展开更多
关键词 Smooth switching mechanism Integral terminal SMc PMSM servo system Disturbance suppression Stator voltage saturation
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Dynamic Analysis of Horizontal Servo System in Suspension Gravity Compensation System
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作者 WANG Wanqing WANG Qingxia +2 位作者 YANG Shuai LI Na HUANG Shunzhou 《Journal of Donghua University(English Edition)》 2025年第1期29-40,共12页
A new suspension gravity compensation system has been developed to alleviate the gravity effects on a two-dimensional(2D)deployable mechanism for ground verification.Considering the rigid-flexible coupling of both the... A new suspension gravity compensation system has been developed to alleviate the gravity effects on a two-dimensional(2D)deployable mechanism for ground verification.Considering the rigid-flexible coupling of both the rotating servo and the suspension system,a multi-body dynamic model simulating their integration is established using Lagrange’s equation.To mitigate instantaneous impact forces due to significant non-plumb effects from passive following in the horizontal direction,an elastic element is added in series with the rope in the vertical suspension system.The dynamic response of this elastic element relative to the rotating servo system is analyzed by the ADAMS software.Simulation results show that the compensating error decreases significantly from 45%to 0.31%when incorporating elastic elements compared to scenarios without such elements.Additionally,low-stiffness elastic elements demonstrate a higher compensating error than high-stiffness ones.A spring with a stiffness coefficient of 6 N/mm is selected in the experiment,ensuring that compensating error meets the design specification of 5%. 展开更多
关键词 suspension gravity compensation passive servo system flexible-rigid coupling model series elastic element
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Parameter identification and high order active disturbance rejection control of electro-hydraulic servo motor system
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作者 WANG Xiaojing GAO Wentao +1 位作者 ZHANG Yuxuan SUN Yuwei 《High Technology Letters》 2025年第3期280-287,共8页
An enhanced least mean square(LMS)error identification algorithm integrated with Kalman filtering is proposed to resolve accuracy degradation induced by nonlinear dynamics and parameter uncertainties in continuous rot... An enhanced least mean square(LMS)error identification algorithm integrated with Kalman filtering is proposed to resolve accuracy degradation induced by nonlinear dynamics and parameter uncertainties in continuous rotary electro-hydraulic servo systems.This enhancement accelerates convergence and improves accuracy compared with traditional LMS.A fifth-order identification mod-el is developed based on valve-controlled hydraulic motors,with parameters identified using Kalman filter state estimation and gradient smoothing.The results indicate that the improved LMS effectively enhances parameter identification.An advanced disturbance rejection controller(ADRC)is de-signed,and its performance is compared with an optimal proportional integral derivative(PID)con-troller through Simulink simulations.The results show that the ADRC fulfills the control specifications and expands the system’s operational bandwidth. 展开更多
关键词 electro-hydraulic servo system tracking differentiator filter minimum mean square error identification advanced disturbance rejection controller nonlinear feedback control law extended state observer parameter optimal proportional integral derivative control
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知识图谱驱动的C++课程BOPPPS+ADCD教学模式探索 被引量:1
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作者 王云艳 《计算机教育》 2026年第1期233-239,共7页
针对传统教学中自主学习和迁移学习不足、高阶计算思维培养不足、评价体系动态反馈不足等问题,提出知识图谱驱动的BOPPPS+ADCD线上线下混合教学模式,以C++程序设计课程为例,阐述如何通过知识图谱引领学生自主学习,并在BOPPPS参与式教学... 针对传统教学中自主学习和迁移学习不足、高阶计算思维培养不足、评价体系动态反馈不足等问题,提出知识图谱驱动的BOPPPS+ADCD线上线下混合教学模式,以C++程序设计课程为例,阐述如何通过知识图谱引领学生自主学习,并在BOPPPS参与式教学环节融入ADCD四阶段强化高阶计算思维训练,构建“教学—评价—改进”智慧评价的动态教学闭环以实现教学动态优化,最后说明教学实践效果。 展开更多
关键词 c++程序设计 知识图谱 BOPPPS+aDcD教学模式 智慧评价
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m^(5)C RNA甲基化修饰与前庭功能异常和老年性耳聋的相关性研究
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作者 周旭 司峰志 《河北医学》 2026年第1期117-122,共6页
目的:探讨m^(5)C RNA甲基化修饰在前庭功能和老年性耳聋中的相关性。方法:总计24只近交系C57BL/6J小鼠,5月龄(成年小鼠组)、12月龄(中年小鼠组)、20月龄(老年小鼠组)各8只。听觉脑干反应阈值分析评价小鼠听力。DCFH-DA(二氯二氢荧光素-... 目的:探讨m^(5)C RNA甲基化修饰在前庭功能和老年性耳聋中的相关性。方法:总计24只近交系C57BL/6J小鼠,5月龄(成年小鼠组)、12月龄(中年小鼠组)、20月龄(老年小鼠组)各8只。听觉脑干反应阈值分析评价小鼠听力。DCFH-DA(二氯二氢荧光素-乙酰乙酸酯)法测定耳蜗和前庭组织ROS水平。TAMRA探针法测定其中组织线粒体DNA的完整性。m^(5)C RNA甲基化修饰免疫共沉淀法分析小鼠耳蜗和前庭组织GRM7 mRNA m^(5)C水平。Western blot法测定其中NSUN2的水平。结果:与成年小鼠组和中年小鼠组相比,老年小鼠组在8、16和32 kHz时的平均ABR阈值升高(P<0.05);耳蜗组织和前庭组织ROS相对水平均升高(P<0.05);含CD mtDNA占比(%)均降低(P<0.05);NSUN2相对表达水平均降低(P<0.05);GRM7 Input mRNA相对表达水平均降低(P<0.05);GRM7 m^(5)C mRNA相对表达水平降低(P<0.05)。老年小鼠组,与耳蜗组织组相比,前庭组织组ROS相对水平较低(P<0.05),GRM7 Input mRNA和GRM7 m^(5)C mRNA相对表达水平较高(P<0.05)。结论:m^(5)C甲基化修饰RNA水平下调及NSUN2表达水平降低与前庭功能和老年性耳聋相关。 展开更多
关键词 老年性耳聋 前庭功能 c57BL/6J小鼠 m^(5)c RNa甲基化修饰 NOP2/Sun RNa甲基转移酶2
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我国2018-2021年A群C群脑膜炎球菌多糖疫苗有效组分回顾性质量趋势分析
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作者 王珊珊 杨越 +4 位作者 刘茹凤 刘倩 李茂光 王斌 徐颖华 《中国医药生物技术》 2026年第2期145-148,共4页
目的 为了进一步评估我国A群C群脑膜炎球菌多糖疫苗(MPV-AC)的质量,对2018-2021年国内两个企业送检MPV-AC关键质量参数进行回顾性质量趋势分析。方法收集2018-2021年国内两个企业送检的MPV-AC批签发资料,对疫苗产品的关键质量参数--有... 目的 为了进一步评估我国A群C群脑膜炎球菌多糖疫苗(MPV-AC)的质量,对2018-2021年国内两个企业送检MPV-AC关键质量参数进行回顾性质量趋势分析。方法收集2018-2021年国内两个企业送检的MPV-AC批签发资料,对疫苗产品的关键质量参数--有效组分A群和C群多糖含量进行趋势分析,同时每年度由批签发承检机构随机抽取一定比例批次疫苗进行A群和C群多糖含量测定,并进行不同年度环比分析。结果 2018-2021年送检两个企业所有批次MPV-AC的生产及检验记录摘要经审查符合标准要求。每年度每个企业送检批次超过100批,合计每年度签发的MPV-AC单次人用疫苗剂量数量均超过3千万。趋势分析结果显示,两个企业在不同年度生产的MPV-AC产品A群和C群多糖含量检测结果趋势整体平稳,几乎全在警戒限(平均值±2SD)以内波动。不同年度的环比统计分析发现,2018-2021年期间在两个企业不同年度之间A群和C群多糖含量结果,以及不同年度随机抽取检测MPV-AC中A群和C群多糖含量检测结果与其企业自检结果之间均无显著性差异(P> 0.05)。结论 两个送检企业的MPV-AC产品中多糖含量稳定,产品质量一致性良好,这些产品的顺利签发不仅为国家免疫规划成功实施奠定了物质基础,也为未来相关疫苗质控的研究提供数据支持。 展开更多
关键词 ac群脑膜炎球菌多糖疫苗 多糖含量 批签发 趋势分析
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慢性萎缩性胃炎患者血清LDH、ApoC1、CHE水平及与疾病严重程度、Hp感染的关系
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作者 张征波 许沁芸 +1 位作者 田秋实 林黎 《检验医学与临床》 2026年第5期640-645,共6页
目的探究慢性萎缩性胃炎(CAG)患者血清乳酸脱氢酶(LDH)、载脂蛋白(Apo)C1、胆碱酯酶(CHE)水平及与患者疾病严重程度、幽门螺旋杆菌(Hp)感染的关系。方法选取2021年6月至2023年6月该院收治的98例CAG患者作为CAG组,根据可操作的与胃癌风... 目的探究慢性萎缩性胃炎(CAG)患者血清乳酸脱氢酶(LDH)、载脂蛋白(Apo)C1、胆碱酯酶(CHE)水平及与患者疾病严重程度、幽门螺旋杆菌(Hp)感染的关系。方法选取2021年6月至2023年6月该院收治的98例CAG患者作为CAG组,根据可操作的与胃癌风险联系的肠上皮化生评估系统将CAG患者分为轻度组、中度组及重度组;根据CAG患者是否发生Hp感染分为Hp感染组及非Hp感染组。另选取同期在该院体检的98例健康体检者作为对照组。收集所有研究对象基线资料。检测所有研究对象血清LDH、APOC1、CHE水平。绘制受试者工作特征(ROC)曲线分析血清LDH、ApoC1、CHE单独及联合检测对CAG患者病情重度的诊断价值。采用多因素Logistic回归分析CAG患者发生Hp感染的影响因素。结果CAG组血清LDH、ApoC1水平均高于对照组,血清CHE水平低于对照组,差异均有统计学意义(P<0.05)。轻度组24例,中度组40例,重度组34例。轻度组、中度组、重度组Hp感染率比较,差异有统计学意义(P<0.05)。轻度组血清LDH、ApoC1水平均低于中度组和重度组,且中度组血清LDH、ApoC1水平均低于重度组,轻度组血清CHE水平高于中度组和重度组,且中度组血清CHE水平高于重度组,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,血清LDH、ApoC1、CHE联合诊断CAG患者病情重度的曲线下面积(AUC)大于LDH、ApoC1、CHE单独诊断的AUC(Z=3.308、2.178、3.462,P=0.001)。Hp感染组65例,非Hp感染组33例。Hp感染组血清LDH、ApoC1水平均高于非Hp感染组,血清CHE水平低于非Hp感染组,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,血清LDH、ApoC1水平升高是CAG患者发生Hp感染的危险因素(P<0.05),血清CHE水平升高是CAG患者发生Hp感染的保护因素(P<0.05)。结论CAG患者血清LDH、APOC1水平均升高,CHE水平降低,可用于评估CAG患者病情,且与Hp感染有关。 展开更多
关键词 慢性萎缩性胃炎 乳酸脱氢酶 载脂蛋白c1 胆碱酯酶 幽门螺杆菌
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儿童肺炎患者外周血白细胞形态分析联合CRP、SAA测定的临床意义
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作者 杨柳 黄洁池 +1 位作者 曹丽坤 李若林 《吉林医学》 2026年第1期60-63,共4页
目的:研究儿童肺炎患者外周血白细胞形态分析联合C反应蛋白(CRP)、血清淀粉样蛋白A(SAA)测定的临床意义。方法:选取2023年7月~2024年6月临床诊断为细菌性及病毒肺炎的200例患儿为研究组观察对象,按病原学分为细菌感染组及病毒感染组,各... 目的:研究儿童肺炎患者外周血白细胞形态分析联合C反应蛋白(CRP)、血清淀粉样蛋白A(SAA)测定的临床意义。方法:选取2023年7月~2024年6月临床诊断为细菌性及病毒肺炎的200例患儿为研究组观察对象,按病原学分为细菌感染组及病毒感染组,各100例。选取同期就诊的100例健康体检儿童为对照组,对所有患儿进行血常规、外周血细胞形态、CRP、SAA检测。实验选取三组患儿一般资料、白细胞计数(WBC)、中性粒细胞比例、淋巴细胞比例、单核细胞比例、血小板(PLT)、炎性指标(CRP、SAA)之间的差异,分析儿童肺炎患者的临床特点及血清实验室检查指标的意义。分析外周血白细胞形态与CRP、SAA测定之间的相关性,对比外周血白细胞形态分析、CRP、SAA测定联合检测与单独检测结果。结果:三组患儿中性粒细胞比例、淋巴细胞比例、单核细胞比例、CRP、SAA差异有统计学意义(P<0.05),外周血白细胞形态分析联合CRP、SAA测定诊断价值高于单独诊断。结果:外周血白细胞形态分析联合CRP、SAA测定在儿童肺炎疾病诊断中有显著价值,有助于临床医师对儿童肺炎进行鉴别。 展开更多
关键词 儿童肺炎 外周血白细胞形态分析 c反应蛋白 血清淀粉样蛋白a 诊断价值
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AKR1C3通过PD1/PD-L1信号通路对乳腺癌细胞恶性生物学行为的干预作用
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作者 宋晶晶 熊伟 +2 位作者 姚淑辉 刘爽 张静 《昆明医科大学学报》 2026年第1期31-38,共8页
目的探索酮还原酶家族1成员C3(aldo-keto reductase family 1 member C3,AKR1C3)对乳腺癌恶性细胞生物学行为的干预作用及对程序性细胞死亡蛋白/程序性死亡-配体1(programmed cell death protein1/programmed death-ligand1,PD-1/PD-L)... 目的探索酮还原酶家族1成员C3(aldo-keto reductase family 1 member C3,AKR1C3)对乳腺癌恶性细胞生物学行为的干预作用及对程序性细胞死亡蛋白/程序性死亡-配体1(programmed cell death protein1/programmed death-ligand1,PD-1/PD-L)通路的影响。方法把MCF-7人乳腺癌细胞中NC组和AKR1C3组分别转染空质粒和AKR1C3质粒,采用MTT法检测转染后24 h、48 h、72 h细胞活力;采用流式细胞技术测定各组细胞的存活率以及早期、晚期凋亡比例;通过Transwell实验对各组细胞的迁移和侵袭能力进行检测;通过Western blot检测各组细胞PD-1、PD-L1、蛋白激酶B(protein kinase b,AKT)蛋白表达水平。使用C57BL/6小鼠构建荷瘤模型,将采用人乳腺癌MCF-7细胞转染NC质粒和AKR1C3质粒进行细胞荷瘤,每3 d测量瘤体积,持续21 d,绘制两组小鼠肿瘤生长曲线,并于实验终点测量肿瘤质量。结果相较于NC组,AKR1C3组细胞活力降低(P<0.05),并且具有时间依赖效应(P<0.05),迁移和侵袭能力降低(P<0.05),早期凋亡和晚期凋亡比例升高(P<0.05),PD-1、PD-L1、AKT蛋白表达水平降低(P<0.05)。动物实验表明,AKR1C3组小鼠肿瘤体积降低,肿瘤质量下降(P<0.05)。结论AKR1C3可以抑制人乳腺癌细胞恶性生物学行为,抑制PD-1/PDL1信号通路蛋白表达。 展开更多
关键词 aKR1c3 人乳腺癌 凋亡 迁移 PD-1/PD-L1通路 侵袭 aKT PPR5
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IGF2BP2通过GPX4/SLC7A11/ACSL4信号轴介导OGD/R诱导的H9c2细胞铁死亡的作用机制
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作者 郭道通 张宗雷 +1 位作者 孟凡华 程云涛 《中国医药导报》 2026年第5期67-73,共7页
目的探究胰岛素样生长因子2-mRNA结合蛋白2(IGF2BP2)在氧糖剥夺/再灌注(OGD/R)诱导的环境下对H9c2细胞铁死亡的保护作用及其机制。方法通过OGD/R诱导建立H9c2细胞损伤模型,并将细胞分为对照组、OGD/R组、OGD/R+si-NC组和OGD/R+si-IGF2BP... 目的探究胰岛素样生长因子2-mRNA结合蛋白2(IGF2BP2)在氧糖剥夺/再灌注(OGD/R)诱导的环境下对H9c2细胞铁死亡的保护作用及其机制。方法通过OGD/R诱导建立H9c2细胞损伤模型,并将细胞分为对照组、OGD/R组、OGD/R+si-NC组和OGD/R+si-IGF2BP2组。通过CCK8评估细胞增殖,流式细胞术检测细胞凋亡和周期,生化试剂盒检测还原型谷胱甘肽(GSH)、丙二醛(MDA)和亚铁离子(Fe^(2+))含量,透射电镜观察细胞线粒体形态变化,Western blot法检测IGF2BP2蛋白及铁死亡相关蛋白谷胱甘肽过氧化物酶4(GPX4)、溶质载体家族7成员11(SLC7A11)和长链酰基辅酶A合成酶4(ACSL4)水平。结果与对照组比较,OGD/R组细胞增殖活性下降且细胞凋亡率升高,细胞中GSH含量下降而MDA和Fe^(2+)含量升高(P<0.05);线粒体出现体积缩小、嵴的数量急剧减少、结构模糊甚至完全消失及外膜破裂等现象;细胞中GPX4、SLC7A11蛋白表达降低,而IGF2BP2和ACSL4蛋白水平升高(P<0.05)。与OGD/R组比较,OGD/R+si-IGF2BP2组细胞增殖活性升高而凋亡率下降,细胞中GSH含量升高而MDA和Fe^(2+)含量下降(P<0.05);线粒体结构恢复正常;细胞中GPX4、SLC7A11蛋白表达升高,而IGF2BP2和ACSL4蛋白水平下降(P<0.05)。结论IGF2BP2下调可能通过调节GPX4/SLC7A11/ACSL4信号轴抑制细胞铁死亡,从而减轻OGD/R诱导的H9c2细胞损伤。 展开更多
关键词 IGF2BP2 氧糖剥夺/再灌注 H9c2细胞 铁死亡
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PARAMETER IDENTIFICATION OF LUGRE FRICTION MODEL FOR FLIGHT SIMULATION SERVO SYSTEM BASED ON ANT COLONY ALGORITHM 被引量:4
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作者 段海滨 王道波 +1 位作者 朱家强 黄向华 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2004年第3期179-183,共5页
In light of the high nonlinearity of LuGre friction model, a novel method based on ant colony algorithm(ACA) for identifying the friction parameters of flight simulation servo system is proposed. ACA is a parallelized... In light of the high nonlinearity of LuGre friction model, a novel method based on ant colony algorithm(ACA) for identifying the friction parameters of flight simulation servo system is proposed. ACA is a parallelized bionic optimization algorithm inspired from the behavior of real ants, and a kind of positive feedback mechanism is adopted in ACA. On the basis of brief introduction of LuGre friction model, a method for identifying the static LuGre friction parameters and the dynamic LuGre friction parameters using ACA is derived. Finally, this new friction parameter identification scheme is applied to a electric-driven flight simulation servo system with high precision. Simulation and application results verify the feasibility and the effectiveness of the scheme. It provides a new way to identify the friction parameters of LuGre model. 展开更多
关键词 parameter identification LuGre friction mo-del flight simulation servo system
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A Kind of PWM Pneumatic Servo Control System——Modulation Methods and Dynamic Responses 被引量:2
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作者 姚晓先 陈汉超 《Journal of Beijing Institute of Technology》 EI CAS 1992年第1期50-58,共9页
Vibrations or dither's are features of the PWM servo control system in their steady outputs. On the grounds of analyses and experiments of a PWM pneumatic servo control system, the paper puts forward four varietie... Vibrations or dither's are features of the PWM servo control system in their steady outputs. On the grounds of analyses and experiments of a PWM pneumatic servo control system, the paper puts forward four varieties of PWM modulation methods, and concludes on the relationship between dithers and the different methods, and then discusses the influence of friction to the dithers. Results from experiments regarding the dynamic and static responses on the given system support the theories presented. 展开更多
关键词 pulse width modulation pneumatic piston actuating mechanisms servo control servo valves FRIcTION vibration effect
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北斗三号MEO卫星B1C/B2a信号单系统精密产品性能评估
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作者 王乐 陆文龙 +3 位作者 解世超 冯瑞瑞 张奋 崔博斌 《测绘地理信息》 2026年第1期157-166,共10页
本文系统评估了北斗三号(BDS-3)MEO卫星B1C/B2a信号在单系统模式下的精密产品性能,从产品生成与应用两个层面,对比分析了BDS-3 MEO单系统与BDS-3MEO/GPS双系统的表现。结果显示:在定轨模糊度固定方面,BDS-3 MEO单系统的非差宽巷与窄巷... 本文系统评估了北斗三号(BDS-3)MEO卫星B1C/B2a信号在单系统模式下的精密产品性能,从产品生成与应用两个层面,对比分析了BDS-3 MEO单系统与BDS-3MEO/GPS双系统的表现。结果显示:在定轨模糊度固定方面,BDS-3 MEO单系统的非差宽巷与窄巷固定率分别为97.97%和90.28%,接近双系统水平;单系统轨道的1D RMS为51.2 mm,略低于双系统,而钟差拟合残差平均值为0.14 ns,序列平滑性优于双系统;在地球自转参数(ERP)方面,X、Y极坐标的RMS分别为39.2μas和27.8μas,X、Y极坐标速率的RMS分别为230.0μas/d和262.2μas/d,日长变化的RMS为203.2μs/d,部分指标略低于双系统,但整体仍满足精密产品要求;单系统天顶总延迟(ZTD)的平均RMSE为6.7 mm;单系统网解PPP的非差模糊度宽巷固定率超过98%,窄巷平均固定率为83%;静态精密单点定位(PPP)中,单系统收敛时间为27.89 min,E、N、U三个方向的精度分别为1.71 cm、2.34 cm和1.25 cm;在框架一致性比对中,单系统的平均尺度变化为0.065 ppb,优于双系统的0.188 ppb,表明在BDS天线对齐至ITRF 2020后单系统产品框架一致性表现良好。研究证实,BDS-3单系统具备独立生成高精度产品的能力,为北斗系统实现自主运行提供了有力支撑。 展开更多
关键词 北斗三号 MEO B1c/B2a 单系统性能评估
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Application of Maximum Probability Approach to the Fault Diagnosis of a Servo System 被引量:3
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作者 马东升 胡佑德 戴凤智 《Journal of Beijing Institute of Technology》 EI CAS 2002年第1期29-32,共4页
In an actual control system, it is often difficult to find out where the faults are if only based on the outside fault phenomena, acquired frequently from a fault system. So the fault diagnosis by outside fault phenom... In an actual control system, it is often difficult to find out where the faults are if only based on the outside fault phenomena, acquired frequently from a fault system. So the fault diagnosis by outside fault phenomena is considered. Based on the theory of fuzzy recognition and fault diagnosis, this method only depends on experience and statistical data to set up fuzzy query relationship between the outside phenomena (fault characters) and the fault sources (fault patterns). From this relationship the most probable fault sources can be obtained, to attain the goal of quick diagnosis. Based on the above approach, the standard fuzzy relationship matrix is stored in the computer as a system database. And experiment data are given to show the fault diagnosis results. The important parameters can be on line sampled and analyzed, and when faults occur, faults can be found, the alarm is given and the controller output is regulated. 展开更多
关键词 maximum probability approach fault diagnosis fault tree servo system
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基于牛病毒性腹泻病毒核心蛋白C间接ELISA抗体检测方法的建立
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作者 贾东鹭 阮武营 +11 位作者 刘飞飞 李星仪 杨澳飞 尹波 陈慧敏 陈建 魏颖 何雷 余祖华 马雪连 丁轲 陈松彪 《中国兽医科学》 北大核心 2026年第1期63-70,共8页
为建立基于牛病毒性腹泻病毒(BVDV)核心蛋白C的间接ELISA检测方法,通过PCR扩增出BVDV C基因,克隆至原核表达载体pET-32a,构建重组质粒pET-32a-C,经IPTG诱导表达纯化后免疫BABL/c小鼠制备多克隆抗体,以C蛋白为包被抗原,建立间接ELISA检... 为建立基于牛病毒性腹泻病毒(BVDV)核心蛋白C的间接ELISA检测方法,通过PCR扩增出BVDV C基因,克隆至原核表达载体pET-32a,构建重组质粒pET-32a-C,经IPTG诱导表达纯化后免疫BABL/c小鼠制备多克隆抗体,以C蛋白为包被抗原,建立间接ELISA检测方法。结果显示,pET-32a-C重组质粒构建成功,重组C蛋白大小约为30 k Da,能够以可溶形式表达,30℃、0.8 mmol/L IPTG诱导6 h可溶性表达最佳;该蛋白免疫BABL/c小鼠后制备血清抗体效价为1∶128000,能够特异性识别C蛋白。该ELISA检测方法的最适条件为:0.5μg/m L抗原37℃包被2 h,10 g/L BSA溶液37℃封闭3 h,1∶16000稀释血清孵育2 h,二抗孵育40 min,避光显色10 min,阴阳性临界值判定标准为0.224,具有良好的特异性和重复性。利用本方法对未免疫接种的疑似BVDV感染的牛血清进行检测,与RT-qPCR核酸检测方法相比,二者阳性符合率达94.73%。上述结果表明,基于C蛋白建立的间接ELISA抗体检测方法特异性强、灵敏度高、重复性好,为BVDV临床诊断及开发基于C蛋白的间接ELISA检测试剂盒奠定了基础。 展开更多
关键词 牛病毒性腹泻病毒 核心蛋白c 原核表达 多克隆抗体 间接ELISa
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Backlash Nonlinear Compensation of Servo Systems Using Backpropagation Neural Networks 被引量:2
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作者 何超 徐立新 张宇河 《Journal of Beijing Institute of Technology》 EI CAS 1999年第3期300-305,共6页
Aim To eliminate the influences of backlash nonlinear characteristics generally existing in servo systems, a nonlinear compensation method using backpropagation neural networks(BPNN) is presented. Methods Based on s... Aim To eliminate the influences of backlash nonlinear characteristics generally existing in servo systems, a nonlinear compensation method using backpropagation neural networks(BPNN) is presented. Methods Based on some weapon tracking servo system, a three layer BPNN was used to off line identify the backlash characteristics, then a nonlinear compensator was designed according to the identification results. Results The simulation results show that the method can effectively get rid of the sustained oscillation(limit cycle) of the system caused by the backlash characteristics, and can improve the system accuracy. Conclusion The method is effective on sloving the problems produced by the backlash characteristics in servo systems, and it can be easily accomplished in engineering. 展开更多
关键词 servo system backlash nonlinear characteristics limit cycle backpropagation neural networks(BPNN) compensation methods
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