Background Uterine aging is a key factor contributing to the deterioration of egg quality and reproductive performance in laying hens.Despite its importance,the molecular mechanisms underlying uterine aging remain poo...Background Uterine aging is a key factor contributing to the deterioration of egg quality and reproductive performance in laying hens.Despite its importance,the molecular mechanisms underlying uterine aging remain poorly defined.This study aimed to characterize gene expression and regulatory changes associated with uterine aging in hens at different life stages.Results Transcriptomic Analysis of uterine tissue from hens aged 350,500,And 700 d revealed dynamic changes in gene expression patterns during aging.A significant upregulation of genes involved in cellular senescence was observed,including increased expression of the p53 signaling pathway And markers associated with inflammation And cell cycle arrest.The most notable changes occurred between 350 And 500 d of age,suggesting this as a critical window for the onset of uterine aging.MicroRNA sequencing identified miR-210a-5p as significantly reduced with age.Target prediction and experimental validation showed that miR-210a-5p directly suppresses the expression of RASL11B,a Ras-like small GTPase that activates the MAPK signaling pathway.In primary uterine epithelial cells,reduced miR-210a-5p levels led to elevated RASL11B expression,increased activation of B-Raf,MEK,and ERK proteins,and enhanced expression of aging-related genes and inflammatory factors.In contrast,overexpression of miR-210a-5p or inhibition of the MAPK pathway delayed senescence and reduced inflammatory signaling.RASL11B overexpression was sufficient to induce aging phenotypes,confirming its central role in promoting uterine cellular aging.Conclusions This study identifies a novel regulatory pathway in which miR-210a-5p modulates uterine aging through the RASL11B-MAPK signaling cascade.The findings provide mechanistic insight into age-related reproductive decline in hens and suggest that targeting this pathway may offer new strategies for maintaining uterine function and extending reproductive lifespan in poultry.展开更多
TGM2A-S钢(/%:0.85C、0.27Si、0.24Mn、0.026P、0.007S、3.98Cr、4.76Mo、6.09W、1.83V、0.12Nb、0.03RE)是在高速钢TGM2A的基础上添加微量铌和稀土开发的新型丝锥用高速钢。TGM2A-S钢的生产工艺流程为25 t EAF-30 t LF-VD(微合金化)-铸...TGM2A-S钢(/%:0.85C、0.27Si、0.24Mn、0.026P、0.007S、3.98Cr、4.76Mo、6.09W、1.83V、0.12Nb、0.03RE)是在高速钢TGM2A的基础上添加微量铌和稀土开发的新型丝锥用高速钢。TGM2A-S钢的生产工艺流程为25 t EAF-30 t LF-VD(微合金化)-铸锭(700 kg)二火锻造(85 mm方)-连轧(Φ8 mm)-冷拉(Φ6.6 mm)-加工丝锥(M6)。结果表明,原工艺:3 t中频感应炉-ESR(280 kg锭)-二火锻造(85 mm方)-连轧(Φ8 mm)-冷拉(Φ6.6 mm)-加工丝锥(M6)生产的TGM2A钢中的O和N含量分别为35.4×10^(-6)和123.6×10^(-6),而改进工艺生产的TGM2A-S钢的O和N含量分别为15.7×10^(-6)和87.7×10^(-6)。TGM2A-S钢的丝锥切削寿命较电渣工艺生产的TGM2A钢提高20%;TGM2A-S钢的淬火晶粒为10.5级,电渣工艺生产的TGM2A钢的晶粒度为10级。展开更多
基金funded by the National Key Research and Development Program of China,grant number 2021YFD1300600Sichuan Science and Technology Program(2024YFNH0025,2022YFYZ0005,2021YFYZ0031)China Agriculture Research System of MOF and MARA(CARS-40).
文摘Background Uterine aging is a key factor contributing to the deterioration of egg quality and reproductive performance in laying hens.Despite its importance,the molecular mechanisms underlying uterine aging remain poorly defined.This study aimed to characterize gene expression and regulatory changes associated with uterine aging in hens at different life stages.Results Transcriptomic Analysis of uterine tissue from hens aged 350,500,And 700 d revealed dynamic changes in gene expression patterns during aging.A significant upregulation of genes involved in cellular senescence was observed,including increased expression of the p53 signaling pathway And markers associated with inflammation And cell cycle arrest.The most notable changes occurred between 350 And 500 d of age,suggesting this as a critical window for the onset of uterine aging.MicroRNA sequencing identified miR-210a-5p as significantly reduced with age.Target prediction and experimental validation showed that miR-210a-5p directly suppresses the expression of RASL11B,a Ras-like small GTPase that activates the MAPK signaling pathway.In primary uterine epithelial cells,reduced miR-210a-5p levels led to elevated RASL11B expression,increased activation of B-Raf,MEK,and ERK proteins,and enhanced expression of aging-related genes and inflammatory factors.In contrast,overexpression of miR-210a-5p or inhibition of the MAPK pathway delayed senescence and reduced inflammatory signaling.RASL11B overexpression was sufficient to induce aging phenotypes,confirming its central role in promoting uterine cellular aging.Conclusions This study identifies a novel regulatory pathway in which miR-210a-5p modulates uterine aging through the RASL11B-MAPK signaling cascade.The findings provide mechanistic insight into age-related reproductive decline in hens and suggest that targeting this pathway may offer new strategies for maintaining uterine function and extending reproductive lifespan in poultry.
