[Objective] The research aimed to isolate the glyphosate-degraded strain and study its degradation characteristics.[Method] A glyphosate-degraded fungal strain A-F02 was isolated from sludge in an aeration tank of a g...[Objective] The research aimed to isolate the glyphosate-degraded strain and study its degradation characteristics.[Method] A glyphosate-degraded fungal strain A-F02 was isolated from sludge in an aeration tank of a glyphosate manufacture.The fungal strain A-F02 was identified according to morphological characteristics and internal transcribed spacer（ITS）region of nuclear ribosomal DNA sequence analysis.The glyphosate-biodegraded characteristics of strain A-F02 and the influencing factors were studied.[Result] The fungal strain A-F02 was identified as Aspergillus oryzae sp..The glyphosate-biodegraded rate was 86.82% in the mineral salt medium with 1 000 mg/L of glyphosate as the sole source of carbon,after being incubated at 30 ℃ and 150 rpm for 7 d.The biodegradation rates and biomass of the A-F02 were the highest under the culture conditions with glucose（0.5%,w/v）,pH 7.5,30 ℃ and glyphosate（1 500 mg/L）.[Conclusion] The research provided the experimental basis for glyphosate-biodegraded enzyme purification.展开更多
本文旨在研究大蒜素(allitridum,All)对SCN5A-F1473S突变的HEK293细胞钠电流降低的逆转作用,为筛选治疗Brugada综合征的新药提供理论依据。采用瞬时转染的方法,将SCN5A-F1473S通道质粒转入HEK293细胞,采用细胞外灌流和共培养模式的方法...本文旨在研究大蒜素(allitridum,All)对SCN5A-F1473S突变的HEK293细胞钠电流降低的逆转作用,为筛选治疗Brugada综合征的新药提供理论依据。采用瞬时转染的方法,将SCN5A-F1473S通道质粒转入HEK293细胞,采用细胞外灌流和共培养模式的方法将All急性和慢性给药,使其作用浓度为30μmol·L^(-1)。采用全细胞膜片钳技术在电压钳模式下记录电流和门控动力学,采用共聚焦显微镜技术和蛋白质免疫印迹技术检测通道蛋白在细胞膜表达,探讨All对SCN5A-F1473S峰钠电流降低的逆转作用。发现All 30μmol·L^(-1)组的HEK293细胞峰钠电流(269.8±16.6 p A/p F)显著增加(P<0.01),几乎接近对照组电流密度(298.2±17.5 p A/p F,P<0.01)。All可使通道稳态失活向更正的方向移动(V_(1/2,inact)恢复至-79.5±2.4 m V,P<0.01),导致失活减慢,并且使通道中间态失活减缓(延长至598.1±22.6 ms,P<0.01)。同时,All增加通道蛋白在细胞膜的分布和表达(与F1473S相比,P<0.01)。All使SCN5A-F1473S突变的细胞电流增加,其主要机制可能与此药物能减慢通道失活及改善突变通道迁移障碍有关。展开更多
We describes a controllable synthesis procedure for growing a-Ee2O3 and Ee3O4 nanowires. High magnetic hematite a-Fe2O3 nanowires are successfully grown on Fe0.5Ni0.5 alloy substrates via an oxide assisted vapor-solid...We describes a controllable synthesis procedure for growing a-Ee2O3 and Ee3O4 nanowires. High magnetic hematite a-Fe2O3 nanowires are successfully grown on Fe0.5Ni0.5 alloy substrates via an oxide assisted vapor-solid process. Experimental results also indicate that previous immersion of the substrates in a solution of oxalic acid causes the grown nanowires to convert gradually into magnetite （Fe3O4） nanowires. Additionally, the saturated state of Fe3O4 nanowires is achieved as the oxalic acid concentration reaches 0.75 mol/L. The average diameter and length of nanowires expands with an increasing operation temperature and the growth density of nanowires accumulates with an increasing gas flux in the vapor-solid process. The growth mechanism of a-Fe2O3 and Fe3O4 nanowires is also discussed. The results demonstrate that the entire synthesis of nanowires can be completed within 2 h.展开更多
文摘[Objective] The research aimed to isolate the glyphosate-degraded strain and study its degradation characteristics.[Method] A glyphosate-degraded fungal strain A-F02 was isolated from sludge in an aeration tank of a glyphosate manufacture.The fungal strain A-F02 was identified according to morphological characteristics and internal transcribed spacer（ITS）region of nuclear ribosomal DNA sequence analysis.The glyphosate-biodegraded characteristics of strain A-F02 and the influencing factors were studied.[Result] The fungal strain A-F02 was identified as Aspergillus oryzae sp..The glyphosate-biodegraded rate was 86.82% in the mineral salt medium with 1 000 mg/L of glyphosate as the sole source of carbon,after being incubated at 30 ℃ and 150 rpm for 7 d.The biodegradation rates and biomass of the A-F02 were the highest under the culture conditions with glucose（0.5%,w/v）,pH 7.5,30 ℃ and glyphosate（1 500 mg/L）.[Conclusion] The research provided the experimental basis for glyphosate-biodegraded enzyme purification.
文摘本文旨在研究大蒜素(allitridum,All)对SCN5A-F1473S突变的HEK293细胞钠电流降低的逆转作用,为筛选治疗Brugada综合征的新药提供理论依据。采用瞬时转染的方法,将SCN5A-F1473S通道质粒转入HEK293细胞,采用细胞外灌流和共培养模式的方法将All急性和慢性给药,使其作用浓度为30μmol·L^(-1)。采用全细胞膜片钳技术在电压钳模式下记录电流和门控动力学,采用共聚焦显微镜技术和蛋白质免疫印迹技术检测通道蛋白在细胞膜表达,探讨All对SCN5A-F1473S峰钠电流降低的逆转作用。发现All 30μmol·L^(-1)组的HEK293细胞峰钠电流(269.8±16.6 p A/p F)显著增加(P<0.01),几乎接近对照组电流密度(298.2±17.5 p A/p F,P<0.01)。All可使通道稳态失活向更正的方向移动(V_(1/2,inact)恢复至-79.5±2.4 m V,P<0.01),导致失活减慢,并且使通道中间态失活减缓(延长至598.1±22.6 ms,P<0.01)。同时,All增加通道蛋白在细胞膜的分布和表达(与F1473S相比,P<0.01)。All使SCN5A-F1473S突变的细胞电流增加,其主要机制可能与此药物能减慢通道失活及改善突变通道迁移障碍有关。
文摘We describes a controllable synthesis procedure for growing a-Ee2O3 and Ee3O4 nanowires. High magnetic hematite a-Fe2O3 nanowires are successfully grown on Fe0.5Ni0.5 alloy substrates via an oxide assisted vapor-solid process. Experimental results also indicate that previous immersion of the substrates in a solution of oxalic acid causes the grown nanowires to convert gradually into magnetite （Fe3O4） nanowires. Additionally, the saturated state of Fe3O4 nanowires is achieved as the oxalic acid concentration reaches 0.75 mol/L. The average diameter and length of nanowires expands with an increasing operation temperature and the growth density of nanowires accumulates with an increasing gas flux in the vapor-solid process. The growth mechanism of a-Fe2O3 and Fe3O4 nanowires is also discussed. The results demonstrate that the entire synthesis of nanowires can be completed within 2 h.
